Silk fibroin/vitreous humor hydrogel scaffold modified by a carbodiimide crosslinker for wound healing
Natural‐derived biomaterials can be used as substrates for the growth, proliferation, and differentiation of cells. In this study, bovine vitreous humor as a biological material was cross‐linked to silk fibroin with different concentration ratios to design a suitable substrate for corneal tissue reg...
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| Veröffentlicht in: | Biopolymers 2024-11, Vol.115 (6), p.e23612-n/a |
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| creator | Rafiei, Sepideh Ghanbari‐Abdolmaleki, Marjan Zeinali, Reza Heidari‐Keshel, Saeed Rahimi, Azam Royanian, Farima Zaeifi, Davood Taheri, Kiana Pourtaghi, Kimia Khaleghi, Maryam Biazar, Esmaeil |
| description | Natural‐derived biomaterials can be used as substrates for the growth, proliferation, and differentiation of cells. In this study, bovine vitreous humor as a biological material was cross‐linked to silk fibroin with different concentration ratios to design a suitable substrate for corneal tissue regeneration. The cross‐linked samples were evaluated with different analyses such as structural, physical (optical, swelling, and degradation), mechanical, and biological (viability, cell adhesion) assays. The results showed that all samples had excellent transparency, especially those with higher silk fibroin content. Increasing the ratio of vitreous humor to silk fibroin decreased mechanical strength and increased swelling and degradation, respectively. There was no significant difference in the toxicity of the samples, and with the increase in vitreous humor ratio, adhesion and cell proliferation increased. Generally, silk fibroin with vitreous humor can provide desirable characteristics as a transparent film for corneal wound healing. |
| doi_str_mv | 10.1002/bip.23612 |
| format | Article |
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In this study, bovine vitreous humor as a biological material was cross‐linked to silk fibroin with different concentration ratios to design a suitable substrate for corneal tissue regeneration. The cross‐linked samples were evaluated with different analyses such as structural, physical (optical, swelling, and degradation), mechanical, and biological (viability, cell adhesion) assays. The results showed that all samples had excellent transparency, especially those with higher silk fibroin content. Increasing the ratio of vitreous humor to silk fibroin decreased mechanical strength and increased swelling and degradation, respectively. There was no significant difference in the toxicity of the samples, and with the increase in vitreous humor ratio, adhesion and cell proliferation increased. Generally, silk fibroin with vitreous humor can provide desirable characteristics as a transparent film for corneal wound healing.</description><identifier>ISSN: 0006-3525</identifier><identifier>ISSN: 1097-0282</identifier><identifier>EISSN: 1097-0282</identifier><identifier>DOI: 10.1002/bip.23612</identifier><identifier>PMID: 38994706</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Animals ; Biocompatibility ; Biocompatible Materials - chemistry ; Biological materials ; Biomaterials ; Biomedical materials ; Carbodiimide ; carbodiimide cross‐linker ; Carbodiimides - chemistry ; Cattle ; Cell adhesion ; Cell Adhesion - drug effects ; cell assays ; Cell differentiation ; Cell proliferation ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cornea ; cornea wound healing ; Cross-Linking Reagents - chemistry ; Degradation ; Differentiation (biology) ; Fibroins - chemistry ; Hydrogels - chemistry ; Mechanical properties ; physical and mechanical properties ; Regeneration (physiology) ; Silk ; Silk fibroin ; Swelling ; Tissue engineering ; Tissue Scaffolds - chemistry ; Toxicity ; Vitreous Body - chemistry ; Vitreous humor ; Vitreous humour ; Wound healing ; Wound Healing - drug effects</subject><ispartof>Biopolymers, 2024-11, Vol.115 (6), p.e23612-n/a</ispartof><rights>2024 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2432-9b70c81aecfaa645f398b43b5734502ec4d9e8ada24b200c9125455764cbfd813</cites><orcidid>0000-0002-2367-2841</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbip.23612$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbip.23612$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38994706$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rafiei, Sepideh</creatorcontrib><creatorcontrib>Ghanbari‐Abdolmaleki, Marjan</creatorcontrib><creatorcontrib>Zeinali, Reza</creatorcontrib><creatorcontrib>Heidari‐Keshel, Saeed</creatorcontrib><creatorcontrib>Rahimi, Azam</creatorcontrib><creatorcontrib>Royanian, Farima</creatorcontrib><creatorcontrib>Zaeifi, Davood</creatorcontrib><creatorcontrib>Taheri, Kiana</creatorcontrib><creatorcontrib>Pourtaghi, Kimia</creatorcontrib><creatorcontrib>Khaleghi, Maryam</creatorcontrib><creatorcontrib>Biazar, Esmaeil</creatorcontrib><title>Silk fibroin/vitreous humor hydrogel scaffold modified by a carbodiimide crosslinker for wound healing</title><title>Biopolymers</title><addtitle>Biopolymers</addtitle><description>Natural‐derived biomaterials can be used as substrates for the growth, proliferation, and differentiation of cells. In this study, bovine vitreous humor as a biological material was cross‐linked to silk fibroin with different concentration ratios to design a suitable substrate for corneal tissue regeneration. The cross‐linked samples were evaluated with different analyses such as structural, physical (optical, swelling, and degradation), mechanical, and biological (viability, cell adhesion) assays. The results showed that all samples had excellent transparency, especially those with higher silk fibroin content. Increasing the ratio of vitreous humor to silk fibroin decreased mechanical strength and increased swelling and degradation, respectively. There was no significant difference in the toxicity of the samples, and with the increase in vitreous humor ratio, adhesion and cell proliferation increased. Generally, silk fibroin with vitreous humor can provide desirable characteristics as a transparent film for corneal wound healing.</description><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biological materials</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Carbodiimide</subject><subject>carbodiimide cross‐linker</subject><subject>Carbodiimides - chemistry</subject><subject>Cattle</subject><subject>Cell adhesion</subject><subject>Cell Adhesion - drug effects</subject><subject>cell assays</subject><subject>Cell differentiation</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cornea</subject><subject>cornea wound healing</subject><subject>Cross-Linking Reagents - chemistry</subject><subject>Degradation</subject><subject>Differentiation (biology)</subject><subject>Fibroins - chemistry</subject><subject>Hydrogels - chemistry</subject><subject>Mechanical properties</subject><subject>physical and mechanical properties</subject><subject>Regeneration (physiology)</subject><subject>Silk</subject><subject>Silk fibroin</subject><subject>Swelling</subject><subject>Tissue engineering</subject><subject>Tissue Scaffolds - chemistry</subject><subject>Toxicity</subject><subject>Vitreous Body - chemistry</subject><subject>Vitreous humor</subject><subject>Vitreous humour</subject><subject>Wound healing</subject><subject>Wound Healing - drug effects</subject><issn>0006-3525</issn><issn>1097-0282</issn><issn>1097-0282</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtPGzEURq2qFUmBRf9AZakbWExy_ZiHly2CFgkJJGBt-UlMZsapzRTl32MS2gVSV1f6dO6new9CXwgsCABd6rBZUNYQ-gHNCYi2AtrRj2gOAE3FalrP0OecHwE4ZwQO0Ix1QvAWmjnyt6FfYx90imFc_glPycUp49U0xIRXW5vig-txNsr72Fs8RBt8cBbrLVbYqKRLEIZgHTYp5tyHce0S9mX5OU6jxSunSvZwhD551Wd3_DYP0f3F-d3Zr-rq-ufl2ferylDOaCV0C6YjyhmvVMNrz0SnOdN1y3gN1BluheuUVZRrCmAEoTWv67bhRnvbEXaITva9mxR_Ty4_ySFk4_peja9_SQatIC0TTVvQb-_QxzilsVwnGSmaKAPCC3W6p3bvJeflJoVBpa0kIF_lyyJf7uQX9utb46QHZ_-Rf20XYLkHnkPvtv9vkj8ub_aVL1znjjk</recordid><startdate>202411</startdate><enddate>202411</enddate><creator>Rafiei, Sepideh</creator><creator>Ghanbari‐Abdolmaleki, Marjan</creator><creator>Zeinali, Reza</creator><creator>Heidari‐Keshel, Saeed</creator><creator>Rahimi, Azam</creator><creator>Royanian, Farima</creator><creator>Zaeifi, Davood</creator><creator>Taheri, Kiana</creator><creator>Pourtaghi, Kimia</creator><creator>Khaleghi, Maryam</creator><creator>Biazar, Esmaeil</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2367-2841</orcidid></search><sort><creationdate>202411</creationdate><title>Silk fibroin/vitreous humor hydrogel scaffold modified by a carbodiimide crosslinker for wound healing</title><author>Rafiei, Sepideh ; Ghanbari‐Abdolmaleki, Marjan ; Zeinali, Reza ; Heidari‐Keshel, Saeed ; Rahimi, Azam ; Royanian, Farima ; Zaeifi, Davood ; Taheri, Kiana ; Pourtaghi, Kimia ; Khaleghi, Maryam ; Biazar, Esmaeil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2432-9b70c81aecfaa645f398b43b5734502ec4d9e8ada24b200c9125455764cbfd813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biological materials</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Carbodiimide</topic><topic>carbodiimide cross‐linker</topic><topic>Carbodiimides - chemistry</topic><topic>Cattle</topic><topic>Cell adhesion</topic><topic>Cell Adhesion - drug effects</topic><topic>cell assays</topic><topic>Cell differentiation</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cornea</topic><topic>cornea wound healing</topic><topic>Cross-Linking Reagents - chemistry</topic><topic>Degradation</topic><topic>Differentiation (biology)</topic><topic>Fibroins - chemistry</topic><topic>Hydrogels - chemistry</topic><topic>Mechanical properties</topic><topic>physical and mechanical properties</topic><topic>Regeneration (physiology)</topic><topic>Silk</topic><topic>Silk fibroin</topic><topic>Swelling</topic><topic>Tissue engineering</topic><topic>Tissue Scaffolds - chemistry</topic><topic>Toxicity</topic><topic>Vitreous Body - chemistry</topic><topic>Vitreous humor</topic><topic>Vitreous humour</topic><topic>Wound healing</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rafiei, Sepideh</creatorcontrib><creatorcontrib>Ghanbari‐Abdolmaleki, Marjan</creatorcontrib><creatorcontrib>Zeinali, Reza</creatorcontrib><creatorcontrib>Heidari‐Keshel, Saeed</creatorcontrib><creatorcontrib>Rahimi, Azam</creatorcontrib><creatorcontrib>Royanian, Farima</creatorcontrib><creatorcontrib>Zaeifi, Davood</creatorcontrib><creatorcontrib>Taheri, Kiana</creatorcontrib><creatorcontrib>Pourtaghi, Kimia</creatorcontrib><creatorcontrib>Khaleghi, Maryam</creatorcontrib><creatorcontrib>Biazar, Esmaeil</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biopolymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rafiei, Sepideh</au><au>Ghanbari‐Abdolmaleki, Marjan</au><au>Zeinali, Reza</au><au>Heidari‐Keshel, Saeed</au><au>Rahimi, Azam</au><au>Royanian, Farima</au><au>Zaeifi, Davood</au><au>Taheri, Kiana</au><au>Pourtaghi, Kimia</au><au>Khaleghi, Maryam</au><au>Biazar, Esmaeil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silk fibroin/vitreous humor hydrogel scaffold modified by a carbodiimide crosslinker for wound healing</atitle><jtitle>Biopolymers</jtitle><addtitle>Biopolymers</addtitle><date>2024-11</date><risdate>2024</risdate><volume>115</volume><issue>6</issue><spage>e23612</spage><epage>n/a</epage><pages>e23612-n/a</pages><issn>0006-3525</issn><issn>1097-0282</issn><eissn>1097-0282</eissn><abstract>Natural‐derived biomaterials can be used as substrates for the growth, proliferation, and differentiation of cells. In this study, bovine vitreous humor as a biological material was cross‐linked to silk fibroin with different concentration ratios to design a suitable substrate for corneal tissue regeneration. The cross‐linked samples were evaluated with different analyses such as structural, physical (optical, swelling, and degradation), mechanical, and biological (viability, cell adhesion) assays. The results showed that all samples had excellent transparency, especially those with higher silk fibroin content. Increasing the ratio of vitreous humor to silk fibroin decreased mechanical strength and increased swelling and degradation, respectively. There was no significant difference in the toxicity of the samples, and with the increase in vitreous humor ratio, adhesion and cell proliferation increased. Generally, silk fibroin with vitreous humor can provide desirable characteristics as a transparent film for corneal wound healing.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>38994706</pmid><doi>10.1002/bip.23612</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-2367-2841</orcidid></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Biocompatibility Biocompatible Materials - chemistry Biological materials Biomaterials Biomedical materials Carbodiimide carbodiimide cross‐linker Carbodiimides - chemistry Cattle Cell adhesion Cell Adhesion - drug effects cell assays Cell differentiation Cell proliferation Cell Proliferation - drug effects Cell Survival - drug effects Cornea cornea wound healing Cross-Linking Reagents - chemistry Degradation Differentiation (biology) Fibroins - chemistry Hydrogels - chemistry Mechanical properties physical and mechanical properties Regeneration (physiology) Silk Silk fibroin Swelling Tissue engineering Tissue Scaffolds - chemistry Toxicity Vitreous Body - chemistry Vitreous humor Vitreous humour Wound healing Wound Healing - drug effects |
| title | Silk fibroin/vitreous humor hydrogel scaffold modified by a carbodiimide crosslinker for wound healing |
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