All-RNA-mediated targeted gene integration in mammalian cells with rationally engineered R2 retrotransposons

All-RNA-mediated targeted gene integration methods, rendering reduced immunogenicity, effective deliverability with non-viral vehicles, and a low risk of random mutagenesis, are urgently needed for next-generation gene addition technologies. Naturally occurring R2 retrotransposons hold promise in th...

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Veröffentlicht in:	Cell 2024-08, Vol.187 (17), p.4674-4689.e18
Hauptverfasser:	Chen, Yangcan, Luo, Shengqiu, Hu, Yanping, Mao, Bangwei, Wang, Xinge, Lu, Zongbao, Shan, Qingtong, Zhang, Jin, Wang, Siqi, Feng, Guihai, Wang, Chenxin, Liang, Chen, Tang, Na, Niu, Rui, Wang, Jiaqiang, Han, Jiabao, Yang, Ning, Wang, Haoyi, Zhou, Qi, Li, Wei
Format:	Artikel
Sprache:	eng
Schlagworte:	AlphaFold2 Animals gene editing gene insertion gene integration Gene Targeting - methods Genetic Engineering - methods HEK293 Cells Humans mammalian cells Mice protein engineering R2 elements Retroelements - genetics retrotransposon RNA - genetics RNA - metabolism RNA donor
Online-Zugang:	Volltext
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