Seamless knockins in Drosophila via CRISPR-triggered single-strand annealing

CRISPR-Cas greatly facilitated the integration of exogenous sequences into specific loci. However, knockin generation in multicellular animals remains challenging, partially due to the complexity of insertion screening. Here, we describe SEED/Harvest, a method to generate knockins in Drosophila, bas...

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Veröffentlicht in:	Developmental cell 2024-10, Vol.59 (19), p.2672-2686.e5
Hauptverfasser:	Aguilar, Gustavo, Bauer, Milena, Vigano, M. Alessandra, Schnider, Sophie T., Brügger, Lukas, Jiménez-Jiménez, Carlos, Guerrero, Isabel, Affolter, Markus
Format:	Artikel
Sprache:	eng
Schlagworte:	ALFA Cas9 conditionally stable CRISPR Drosophila knockin nanobody SEED SSA tissue specific
Online-Zugang:	Volltext
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