Differential polyvalent passive immune protection of egg yolk antibodies (IgY) against live and inactivated Vibrio fluvialis in fish
Egg yolk antibodies (IgY) can be prepared in large quantities and economically, and have potential value as polyvalent passive vaccines (against multiple bacteria) in aquaculture. This study prepared live and inactivated Vibrio fluvialis IgY and immunized Carassius auratus prior to infection with V....
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| Veröffentlicht in: | Fish & shellfish immunology 2024-08, Vol.151, p.109751, Article 109751 |
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| container_title | Fish & shellfish immunology |
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| creator | Liu, Xiang Xiao, Huihui Cui, Pan Chen, Jing Chao, Jia Wu, Xiaoqing Lu, Juan Zhang, Xiaoying Xu, Gaoxiao Liu, Yong |
| description | Egg yolk antibodies (IgY) can be prepared in large quantities and economically, and have potential value as polyvalent passive vaccines (against multiple bacteria) in aquaculture. This study prepared live and inactivated Vibrio fluvialis IgY and immunized Carassius auratus prior to infection with V. fluvialis and Aeromonas hydrophila. The results showed that the two IgY antibodies hold effective passive protective rates against V. fluvialis and A. hydrophila in C. auratus. Further, the serum of C. auratus recognized the two bacteria in vitro, with a decrease in the bacteria content of the kidney. The phagocytic activity of C. auratus plasma was enhanced, with a decrease in the expression of inflammatory and antioxidant factors. Pathological sections showed that the kidney, spleen, and intestinal tissue structures were intact, and apoptosis and DNA damage decreased in kidney cells. Moreover, the immunoprotection conferred by the live V. fluvialis IgY was higher than that of the inactivated IgY. Addition, live V. fluvialis immunity induced IgY antibodies against outer membrane proteins of V. fluvialis were more than inactivated V. fluvialis immunity. Furthermore, heterologous immune bacteria will not cause infection, so V. fluvialis can be used to immunize chickens to obtain a large amount of IgY antibody. These findings suggest that the passive immunization effect of live bacterial IgY antibody on fish is significantly better than that of inactivated bacterial antibody, and the live V. fluvialis IgY hold potential value as polyvalent passive vaccines in aquaculture.
•The IgY antibodies against live and inactivated Vibrio fluvialis were prepared.•The two IgY antibodies can activate the innate immune responses in Carassius auratus.•The two IgY antibodies have the passive and passive cross-protective abilities to C. auratus.•The IgY antibody against live V. fluvialis can be considered polyvalent passive vaccine candidates in aquaculture. |
| doi_str_mv | 10.1016/j.fsi.2024.109751 |
| format | Article |
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•The IgY antibodies against live and inactivated Vibrio fluvialis were prepared.•The two IgY antibodies can activate the innate immune responses in Carassius auratus.•The two IgY antibodies have the passive and passive cross-protective abilities to C. auratus.•The IgY antibody against live V. fluvialis can be considered polyvalent passive vaccine candidates in aquaculture.</description><identifier>ISSN: 1050-4648</identifier><identifier>ISSN: 1095-9947</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2024.109751</identifier><identifier>PMID: 38971349</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Aquaculture ; Egg yolk antibody ; Passive immune vaccine ; Polyvalent vaccine ; Vibrio fluvialis</subject><ispartof>Fish & shellfish immunology, 2024-08, Vol.151, p.109751, Article 109751</ispartof><rights>2024 Elsevier Ltd</rights><rights>Copyright © 2024. Published by Elsevier Ltd.</rights><rights>Copyright © 2024 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c235t-c5ea83f7e46d361f99b1348821d47e42aabd5723b255a9c223af4ac68ce957ff3</cites><orcidid>0000-0002-8208-0571</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fsi.2024.109751$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38971349$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Xiang</creatorcontrib><creatorcontrib>Xiao, Huihui</creatorcontrib><creatorcontrib>Cui, Pan</creatorcontrib><creatorcontrib>Chen, Jing</creatorcontrib><creatorcontrib>Chao, Jia</creatorcontrib><creatorcontrib>Wu, Xiaoqing</creatorcontrib><creatorcontrib>Lu, Juan</creatorcontrib><creatorcontrib>Zhang, Xiaoying</creatorcontrib><creatorcontrib>Xu, Gaoxiao</creatorcontrib><creatorcontrib>Liu, Yong</creatorcontrib><title>Differential polyvalent passive immune protection of egg yolk antibodies (IgY) against live and inactivated Vibrio fluvialis in fish</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>Egg yolk antibodies (IgY) can be prepared in large quantities and economically, and have potential value as polyvalent passive vaccines (against multiple bacteria) in aquaculture. This study prepared live and inactivated Vibrio fluvialis IgY and immunized Carassius auratus prior to infection with V. fluvialis and Aeromonas hydrophila. The results showed that the two IgY antibodies hold effective passive protective rates against V. fluvialis and A. hydrophila in C. auratus. Further, the serum of C. auratus recognized the two bacteria in vitro, with a decrease in the bacteria content of the kidney. The phagocytic activity of C. auratus plasma was enhanced, with a decrease in the expression of inflammatory and antioxidant factors. Pathological sections showed that the kidney, spleen, and intestinal tissue structures were intact, and apoptosis and DNA damage decreased in kidney cells. Moreover, the immunoprotection conferred by the live V. fluvialis IgY was higher than that of the inactivated IgY. Addition, live V. fluvialis immunity induced IgY antibodies against outer membrane proteins of V. fluvialis were more than inactivated V. fluvialis immunity. Furthermore, heterologous immune bacteria will not cause infection, so V. fluvialis can be used to immunize chickens to obtain a large amount of IgY antibody. These findings suggest that the passive immunization effect of live bacterial IgY antibody on fish is significantly better than that of inactivated bacterial antibody, and the live V. fluvialis IgY hold potential value as polyvalent passive vaccines in aquaculture.
•The IgY antibodies against live and inactivated Vibrio fluvialis were prepared.•The two IgY antibodies can activate the innate immune responses in Carassius auratus.•The two IgY antibodies have the passive and passive cross-protective abilities to C. auratus.•The IgY antibody against live V. fluvialis can be considered polyvalent passive vaccine candidates in aquaculture.</description><subject>Aquaculture</subject><subject>Egg yolk antibody</subject><subject>Passive immune vaccine</subject><subject>Polyvalent vaccine</subject><subject>Vibrio fluvialis</subject><issn>1050-4648</issn><issn>1095-9947</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kDtvFDEUhS1ERELgB9Agl6GYjV_zsKhQwiNSJJoEicry2NfLXWbGiz0z0vb8cLzaQEnle-1zjnU-Qt5wtuGMN9e7Tci4EUyosuu25s_IRRnqSmvVPj_ONatUo7pz8jLnHWOskQ17Qc5lp1sulb4gv28xBEgwzWgHuo_DYbVD2eje5owrUBzHZQK6T3EGN2OcaAwUtlt6iMNPaouvjx4h06u77fd31G4tTnmmw9FrJ09xssW22hk8_YZ9wkjDsKzlN8zlkQbMP16Rs2CHDK-fzkvy-Onjw82X6v7r57ubD_eVE7KeK1eD7WRoQTVeNjxo3ZcSXSe4V-VSWNv7uhWyF3VttRNC2qCsazoHum5DkJfk6pRb2vxaIM9mxOxgGOwEcclGsrZRQmjGi5SfpC7FnBMEs0842nQwnJkjfLMzBb45wjcn-MXz9il-6Ufw_xx_aRfB-5MASskVIZnsECYHHlOBa3zE_8T_Aca0lxw</recordid><startdate>20240801</startdate><enddate>20240801</enddate><creator>Liu, Xiang</creator><creator>Xiao, Huihui</creator><creator>Cui, Pan</creator><creator>Chen, Jing</creator><creator>Chao, Jia</creator><creator>Wu, Xiaoqing</creator><creator>Lu, Juan</creator><creator>Zhang, Xiaoying</creator><creator>Xu, Gaoxiao</creator><creator>Liu, Yong</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8208-0571</orcidid></search><sort><creationdate>20240801</creationdate><title>Differential polyvalent passive immune protection of egg yolk antibodies (IgY) against live and inactivated Vibrio fluvialis in fish</title><author>Liu, Xiang ; Xiao, Huihui ; Cui, Pan ; Chen, Jing ; Chao, Jia ; Wu, Xiaoqing ; Lu, Juan ; Zhang, Xiaoying ; Xu, Gaoxiao ; Liu, Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c235t-c5ea83f7e46d361f99b1348821d47e42aabd5723b255a9c223af4ac68ce957ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aquaculture</topic><topic>Egg yolk antibody</topic><topic>Passive immune vaccine</topic><topic>Polyvalent vaccine</topic><topic>Vibrio fluvialis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Xiang</creatorcontrib><creatorcontrib>Xiao, Huihui</creatorcontrib><creatorcontrib>Cui, Pan</creatorcontrib><creatorcontrib>Chen, Jing</creatorcontrib><creatorcontrib>Chao, Jia</creatorcontrib><creatorcontrib>Wu, Xiaoqing</creatorcontrib><creatorcontrib>Lu, Juan</creatorcontrib><creatorcontrib>Zhang, Xiaoying</creatorcontrib><creatorcontrib>Xu, Gaoxiao</creatorcontrib><creatorcontrib>Liu, Yong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Xiang</au><au>Xiao, Huihui</au><au>Cui, Pan</au><au>Chen, Jing</au><au>Chao, Jia</au><au>Wu, Xiaoqing</au><au>Lu, Juan</au><au>Zhang, Xiaoying</au><au>Xu, Gaoxiao</au><au>Liu, Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential polyvalent passive immune protection of egg yolk antibodies (IgY) against live and inactivated Vibrio fluvialis in fish</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2024-08-01</date><risdate>2024</risdate><volume>151</volume><spage>109751</spage><pages>109751-</pages><artnum>109751</artnum><issn>1050-4648</issn><issn>1095-9947</issn><eissn>1095-9947</eissn><abstract>Egg yolk antibodies (IgY) can be prepared in large quantities and economically, and have potential value as polyvalent passive vaccines (against multiple bacteria) in aquaculture. This study prepared live and inactivated Vibrio fluvialis IgY and immunized Carassius auratus prior to infection with V. fluvialis and Aeromonas hydrophila. The results showed that the two IgY antibodies hold effective passive protective rates against V. fluvialis and A. hydrophila in C. auratus. Further, the serum of C. auratus recognized the two bacteria in vitro, with a decrease in the bacteria content of the kidney. The phagocytic activity of C. auratus plasma was enhanced, with a decrease in the expression of inflammatory and antioxidant factors. Pathological sections showed that the kidney, spleen, and intestinal tissue structures were intact, and apoptosis and DNA damage decreased in kidney cells. Moreover, the immunoprotection conferred by the live V. fluvialis IgY was higher than that of the inactivated IgY. Addition, live V. fluvialis immunity induced IgY antibodies against outer membrane proteins of V. fluvialis were more than inactivated V. fluvialis immunity. Furthermore, heterologous immune bacteria will not cause infection, so V. fluvialis can be used to immunize chickens to obtain a large amount of IgY antibody. These findings suggest that the passive immunization effect of live bacterial IgY antibody on fish is significantly better than that of inactivated bacterial antibody, and the live V. fluvialis IgY hold potential value as polyvalent passive vaccines in aquaculture.
•The IgY antibodies against live and inactivated Vibrio fluvialis were prepared.•The two IgY antibodies can activate the innate immune responses in Carassius auratus.•The two IgY antibodies have the passive and passive cross-protective abilities to C. auratus.•The IgY antibody against live V. fluvialis can be considered polyvalent passive vaccine candidates in aquaculture.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>38971349</pmid><doi>10.1016/j.fsi.2024.109751</doi><orcidid>https://orcid.org/0000-0002-8208-0571</orcidid></addata></record> |
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| subjects | Aquaculture Egg yolk antibody Passive immune vaccine Polyvalent vaccine Vibrio fluvialis |
| title | Differential polyvalent passive immune protection of egg yolk antibodies (IgY) against live and inactivated Vibrio fluvialis in fish |
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