Surface display provides an efficient expression system for production of recombinant proteins and bacterial whole cell biosensor in E. coli

A novel bacterial display vector based on Escherichia coli has been engineered for recombinant protein production and purification. Accordingly, a construct harboring the enhanced green ﬂuorescent protein (EGFP) and the ice nucleation protein (INP) was designed to produce EGFP via the surface displa...

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Veröffentlicht in:	Analytical biochemistry 2024-11, Vol.694, p.115599, Article 115599
Hauptverfasser:	Ramezani Khorsand, Fereshteh, Hakimi Naeini, Saghi, Molakarimi, Maryam, Dehnavi, Ehsan, Zeinoddini, Mehdi, Sajedi, Reza H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Outer Membrane Proteins - genetics Bacterial Outer Membrane Proteins - metabolism Biosensing Techniques - methods Cell surface display Copper Cu2+ detection EGFP Escherichia coli - genetics Escherichia coli - metabolism Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - metabolism
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