Self‐Replication Without Hydrogen‐Bonds: An Exobiotic Design

Life on Earth uses DNA as the central template for self‐replication, genetic encoding, and information transfer. However, there are no physical laws precluding life's existence elsewhere in space, and alternative life forms may not need DNA. In the search for exobiology, knowing what to look for as a biosignature remains a challenge – especially if it is not from the obvious list of biologic building blocks. Clues from chemicals recently discovered on Mars and in the Taurus Molecular Cloud 1 (TMC‐1), show that intriguing organic compounds exist beyond Earth, which could provide a starting point for unconventional exobiotic designs. Here we present a new potential self‐replicating system with structural similarities to recently discovered compounds on Mars and TMC‐1. Rather than using DNA's hydrogen‐bonding motif for reliable base‐paring, our design employs sulfur‐nitrogen interactions to selectively template unique benzothiadiazole units in sequence. We synthesized and studied two versions of this system, one reversible and the other irreversible, and found experimental evidence of self‐replication in d‐chloroform solvent. These results are part of a larger pursuit in our lab for developing a basis for a potential exobiological system using starting blocks closely related to these cosmic compounds. Exobiotic self‐replication: When sulfur is positioned between the two nitrogens of benzothiadiazole, it can engage in edge‐on molecular recognition events. We present a new potential self‐replicating system based on this interaction, and evidence for autocatalysis. The designs have implications for exobiology, due to their cosmic similarities and potential for genetic capacity.