Isolation, Expansion, and Nucleofection of Neural Stem Cells from Adult Murine Subventricular Zone

Isolation and expansion of neural stem cells (NSCs) from the subventricular zone (SVZ) of the adult mouse brain can be achieved in a medium supplemented with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) as mitogens, producing clonal aggregates known as neurospheres. This i...

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Veröffentlicht in:	Journal of visualized experiments 2024-06 (208)
Hauptverfasser:	Jiménez-Villalba, Esteban, Lázaro-Carot, Laura, Mateos-Martínez, Carmen M, Díaz-Moncho, Jennifer, Samper-Llavador, Daniel, Igual-López, Marta, Planells, Jordi, Ferrón, Sacri R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cytological Techniques - methods Lateral Ventricles - cytology Mice Neural Stem Cells - cytology Neural Stem Cells - metabolism Transfection - methods
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