Simultaneous SERS-decoding detection of multiple pathogens in drinking water with home-made portable double-layer filtration and concentration device
The engineering of a home-made portable double-layer filtration and concentration device with the common syringe for rapid analysis of water samples is reported. The core elements of the device were two installed filtration membranes with different pore sizes for respective functions. The upper filt...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2024-07, Vol.191 (7), p.429, Article 429 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Wu, Huqi Gao, Yan Chen, Qi Yao, Li Yao, Bangben Yang, Jielin Chen, Wei |
| description | The engineering of a home-made portable double-layer filtration and concentration device with the common syringe for rapid analysis of water samples is reported. The core elements of the device were two installed filtration membranes with different pore sizes for respective functions. The upper filtration membrane was used for preliminary intercepting large interfering impurities (
interception membrane
), while the lower filtration membrane was used for collecting multiple target pathogens (
enrichment membrane
) for determination. This combination can make the contaminated environmental water, exemplified by surface water, filtrated quickly through the device and just retained the target bacteria of
Escherichia coli
O157:H7,
Staphylococcus aureus
, and
Listeria monocytogenes
on the lower enrichment membrane. Integrating with surface-enhanced Raman spectra (SERS) platform to decode the SERS-Tags (SERS-Tag
CVa
, SERS-Tag
R6G
, and SERS-Tag
MB
) already labeled on each of the enriched bacteria based the antibody-mediated immuno-recognition effect, fast separation, concentration, and detection of multiple pathogenic bacteria from the bulk of contaminated environmental water were realized. Results show that within 30 min, all target bacteria in the lake water can be simultaneously and accurately measured in the range from 10
1
to 10
6
CFU mL
−1
with detection limit of 10.0 CFU mL
−1
without any pre-culture procedures. This work highlights the simplicity, rapidness, cheapness, selectivity, and the robustness of the constructed method for simultaneous detecting multiple pathogens in aqueous samples. This protocol opens a new avenue for facilitating the development of versatile analytical tools for drinking water and food safety monitoring in underdeveloped or developing countries.
Graphical abstract |
| doi_str_mv | 10.1007/s00604-024-06492-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3073653969</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3077092798</sourcerecordid><originalsourceid>FETCH-LOGICAL-c256t-9dc03b44a8bb39857dd574308d9c0f2788f056d67b3c3159176aec2a5695a5b23</originalsourceid><addsrcrecordid>eNp9kcFuFSEUhonR2Gv1BVwYEjdu0DMwwLA0Ta0mTUy8up4wwNxLnYErMG36IH1fuZ1WExcuyEk43__DOT9Crxt43wDIDxlAQEuA1iNaRQk8QZumZYJwkOwp2gBQQZiQ9AS9yPkKoJGCts_RCetUS1XDN-hu6-dlKjq4uGS8Pf-2JdaZaH3YYeuKM8XHgOOIj5Q_TA4fdNnHnQsZ-4Bt8uHnkb3RxSV848se7-PsyKxtRWMqeqgaG5dayKRvKzT6qSR976uDxSYG48LjjXXX3riX6Nmop-xePdRT9OPT-fezz-Ty68WXs4-XxFAuClHWABvaVnfDwFTHpbVctgw6qwyMVHbdCFxYIQdmWMNVHV87QzUXims-UHaK3q2-hxR_LS6XfvbZuGla99GzukfBmRKqom__Qa_ikkL93ZGSoKhUXaXoSpkUc05u7A_Jzzrd9g30x9D6NbS-htbfh9ZDFb15sF6G2dk_kseUKsBWINdW2Ln09-3_2P4G_6ukXQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3077092798</pqid></control><display><type>article</type><title>Simultaneous SERS-decoding detection of multiple pathogens in drinking water with home-made portable double-layer filtration and concentration device</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Wu, Huqi ; Gao, Yan ; Chen, Qi ; Yao, Li ; Yao, Bangben ; Yang, Jielin ; Chen, Wei</creator><creatorcontrib>Wu, Huqi ; Gao, Yan ; Chen, Qi ; Yao, Li ; Yao, Bangben ; Yang, Jielin ; Chen, Wei</creatorcontrib><description>The engineering of a home-made portable double-layer filtration and concentration device with the common syringe for rapid analysis of water samples is reported. The core elements of the device were two installed filtration membranes with different pore sizes for respective functions. The upper filtration membrane was used for preliminary intercepting large interfering impurities (
interception membrane
), while the lower filtration membrane was used for collecting multiple target pathogens (
enrichment membrane
) for determination. This combination can make the contaminated environmental water, exemplified by surface water, filtrated quickly through the device and just retained the target bacteria of
Escherichia coli
O157:H7,
Staphylococcus aureus
, and
Listeria monocytogenes
on the lower enrichment membrane. Integrating with surface-enhanced Raman spectra (SERS) platform to decode the SERS-Tags (SERS-Tag
CVa
, SERS-Tag
R6G
, and SERS-Tag
MB
) already labeled on each of the enriched bacteria based the antibody-mediated immuno-recognition effect, fast separation, concentration, and detection of multiple pathogenic bacteria from the bulk of contaminated environmental water were realized. Results show that within 30 min, all target bacteria in the lake water can be simultaneously and accurately measured in the range from 10
1
to 10
6
CFU mL
−1
with detection limit of 10.0 CFU mL
−1
without any pre-culture procedures. This work highlights the simplicity, rapidness, cheapness, selectivity, and the robustness of the constructed method for simultaneous detecting multiple pathogens in aqueous samples. This protocol opens a new avenue for facilitating the development of versatile analytical tools for drinking water and food safety monitoring in underdeveloped or developing countries.
Graphical abstract</description><identifier>ISSN: 0026-3672</identifier><identifier>ISSN: 1436-5073</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-024-06492-0</identifier><identifier>PMID: 38942915</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Analytical Chemistry ; Bacteria ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Coliforms ; Decoding ; Developing countries ; Drinking water ; Drinking Water - microbiology ; E coli ; Enrichment ; Escherichia coli O157 - isolation & purification ; Filtration ; Filtration - instrumentation ; Interception ; LDCs ; Limit of Detection ; Listeria monocytogenes - isolation & purification ; Membranes ; Metal Nanoparticles - chemistry ; Microengineering ; Nanochemistry ; Nanotechnology ; Original Paper ; Pathogens ; Portable equipment ; Raman spectra ; Spectrum Analysis, Raman - methods ; Staphylococcus aureus - isolation & purification ; Surface water ; Water Microbiology ; Water sampling</subject><ispartof>Mikrochimica acta (1966), 2024-07, Vol.191 (7), p.429, Article 429</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c256t-9dc03b44a8bb39857dd574308d9c0f2788f056d67b3c3159176aec2a5695a5b23</cites><orcidid>0000-0003-3763-1183</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-024-06492-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-024-06492-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38942915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Huqi</creatorcontrib><creatorcontrib>Gao, Yan</creatorcontrib><creatorcontrib>Chen, Qi</creatorcontrib><creatorcontrib>Yao, Li</creatorcontrib><creatorcontrib>Yao, Bangben</creatorcontrib><creatorcontrib>Yang, Jielin</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><title>Simultaneous SERS-decoding detection of multiple pathogens in drinking water with home-made portable double-layer filtration and concentration device</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>The engineering of a home-made portable double-layer filtration and concentration device with the common syringe for rapid analysis of water samples is reported. The core elements of the device were two installed filtration membranes with different pore sizes for respective functions. The upper filtration membrane was used for preliminary intercepting large interfering impurities (
interception membrane
), while the lower filtration membrane was used for collecting multiple target pathogens (
enrichment membrane
) for determination. This combination can make the contaminated environmental water, exemplified by surface water, filtrated quickly through the device and just retained the target bacteria of
Escherichia coli
O157:H7,
Staphylococcus aureus
, and
Listeria monocytogenes
on the lower enrichment membrane. Integrating with surface-enhanced Raman spectra (SERS) platform to decode the SERS-Tags (SERS-Tag
CVa
, SERS-Tag
R6G
, and SERS-Tag
MB
) already labeled on each of the enriched bacteria based the antibody-mediated immuno-recognition effect, fast separation, concentration, and detection of multiple pathogenic bacteria from the bulk of contaminated environmental water were realized. Results show that within 30 min, all target bacteria in the lake water can be simultaneously and accurately measured in the range from 10
1
to 10
6
CFU mL
−1
with detection limit of 10.0 CFU mL
−1
without any pre-culture procedures. This work highlights the simplicity, rapidness, cheapness, selectivity, and the robustness of the constructed method for simultaneous detecting multiple pathogens in aqueous samples. This protocol opens a new avenue for facilitating the development of versatile analytical tools for drinking water and food safety monitoring in underdeveloped or developing countries.
Graphical abstract</description><subject>Analytical Chemistry</subject><subject>Bacteria</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Coliforms</subject><subject>Decoding</subject><subject>Developing countries</subject><subject>Drinking water</subject><subject>Drinking Water - microbiology</subject><subject>E coli</subject><subject>Enrichment</subject><subject>Escherichia coli O157 - isolation & purification</subject><subject>Filtration</subject><subject>Filtration - instrumentation</subject><subject>Interception</subject><subject>LDCs</subject><subject>Limit of Detection</subject><subject>Listeria monocytogenes - isolation & purification</subject><subject>Membranes</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Pathogens</subject><subject>Portable equipment</subject><subject>Raman spectra</subject><subject>Spectrum Analysis, Raman - methods</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Surface water</subject><subject>Water Microbiology</subject><subject>Water sampling</subject><issn>0026-3672</issn><issn>1436-5073</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFuFSEUhonR2Gv1BVwYEjdu0DMwwLA0Ta0mTUy8up4wwNxLnYErMG36IH1fuZ1WExcuyEk43__DOT9Crxt43wDIDxlAQEuA1iNaRQk8QZumZYJwkOwp2gBQQZiQ9AS9yPkKoJGCts_RCetUS1XDN-hu6-dlKjq4uGS8Pf-2JdaZaH3YYeuKM8XHgOOIj5Q_TA4fdNnHnQsZ-4Bt8uHnkb3RxSV848se7-PsyKxtRWMqeqgaG5dayKRvKzT6qSR976uDxSYG48LjjXXX3riX6Nmop-xePdRT9OPT-fezz-Ty68WXs4-XxFAuClHWABvaVnfDwFTHpbVctgw6qwyMVHbdCFxYIQdmWMNVHV87QzUXims-UHaK3q2-hxR_LS6XfvbZuGla99GzukfBmRKqom__Qa_ikkL93ZGSoKhUXaXoSpkUc05u7A_Jzzrd9g30x9D6NbS-htbfh9ZDFb15sF6G2dk_kseUKsBWINdW2Ln09-3_2P4G_6ukXQ</recordid><startdate>20240701</startdate><enddate>20240701</enddate><creator>Wu, Huqi</creator><creator>Gao, Yan</creator><creator>Chen, Qi</creator><creator>Yao, Li</creator><creator>Yao, Bangben</creator><creator>Yang, Jielin</creator><creator>Chen, Wei</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3763-1183</orcidid></search><sort><creationdate>20240701</creationdate><title>Simultaneous SERS-decoding detection of multiple pathogens in drinking water with home-made portable double-layer filtration and concentration device</title><author>Wu, Huqi ; Gao, Yan ; Chen, Qi ; Yao, Li ; Yao, Bangben ; Yang, Jielin ; Chen, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-9dc03b44a8bb39857dd574308d9c0f2788f056d67b3c3159176aec2a5695a5b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Analytical Chemistry</topic><topic>Bacteria</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Coliforms</topic><topic>Decoding</topic><topic>Developing countries</topic><topic>Drinking water</topic><topic>Drinking Water - microbiology</topic><topic>E coli</topic><topic>Enrichment</topic><topic>Escherichia coli O157 - isolation & purification</topic><topic>Filtration</topic><topic>Filtration - instrumentation</topic><topic>Interception</topic><topic>LDCs</topic><topic>Limit of Detection</topic><topic>Listeria monocytogenes - isolation & purification</topic><topic>Membranes</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Pathogens</topic><topic>Portable equipment</topic><topic>Raman spectra</topic><topic>Spectrum Analysis, Raman - methods</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Surface water</topic><topic>Water Microbiology</topic><topic>Water sampling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Huqi</creatorcontrib><creatorcontrib>Gao, Yan</creatorcontrib><creatorcontrib>Chen, Qi</creatorcontrib><creatorcontrib>Yao, Li</creatorcontrib><creatorcontrib>Yao, Bangben</creatorcontrib><creatorcontrib>Yang, Jielin</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Huqi</au><au>Gao, Yan</au><au>Chen, Qi</au><au>Yao, Li</au><au>Yao, Bangben</au><au>Yang, Jielin</au><au>Chen, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous SERS-decoding detection of multiple pathogens in drinking water with home-made portable double-layer filtration and concentration device</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2024-07-01</date><risdate>2024</risdate><volume>191</volume><issue>7</issue><spage>429</spage><pages>429-</pages><artnum>429</artnum><issn>0026-3672</issn><issn>1436-5073</issn><eissn>1436-5073</eissn><abstract>The engineering of a home-made portable double-layer filtration and concentration device with the common syringe for rapid analysis of water samples is reported. The core elements of the device were two installed filtration membranes with different pore sizes for respective functions. The upper filtration membrane was used for preliminary intercepting large interfering impurities (
interception membrane
), while the lower filtration membrane was used for collecting multiple target pathogens (
enrichment membrane
) for determination. This combination can make the contaminated environmental water, exemplified by surface water, filtrated quickly through the device and just retained the target bacteria of
Escherichia coli
O157:H7,
Staphylococcus aureus
, and
Listeria monocytogenes
on the lower enrichment membrane. Integrating with surface-enhanced Raman spectra (SERS) platform to decode the SERS-Tags (SERS-Tag
CVa
, SERS-Tag
R6G
, and SERS-Tag
MB
) already labeled on each of the enriched bacteria based the antibody-mediated immuno-recognition effect, fast separation, concentration, and detection of multiple pathogenic bacteria from the bulk of contaminated environmental water were realized. Results show that within 30 min, all target bacteria in the lake water can be simultaneously and accurately measured in the range from 10
1
to 10
6
CFU mL
−1
with detection limit of 10.0 CFU mL
−1
without any pre-culture procedures. This work highlights the simplicity, rapidness, cheapness, selectivity, and the robustness of the constructed method for simultaneous detecting multiple pathogens in aqueous samples. This protocol opens a new avenue for facilitating the development of versatile analytical tools for drinking water and food safety monitoring in underdeveloped or developing countries.
Graphical abstract</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>38942915</pmid><doi>10.1007/s00604-024-06492-0</doi><orcidid>https://orcid.org/0000-0003-3763-1183</orcidid></addata></record> |
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| ispartof | Mikrochimica acta (1966), 2024-07, Vol.191 (7), p.429, Article 429 |
| issn | 0026-3672 1436-5073 1436-5073 |
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| subjects | Analytical Chemistry Bacteria Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Coliforms Decoding Developing countries Drinking water Drinking Water - microbiology E coli Enrichment Escherichia coli O157 - isolation & purification Filtration Filtration - instrumentation Interception LDCs Limit of Detection Listeria monocytogenes - isolation & purification Membranes Metal Nanoparticles - chemistry Microengineering Nanochemistry Nanotechnology Original Paper Pathogens Portable equipment Raman spectra Spectrum Analysis, Raman - methods Staphylococcus aureus - isolation & purification Surface water Water Microbiology Water sampling |
| title | Simultaneous SERS-decoding detection of multiple pathogens in drinking water with home-made portable double-layer filtration and concentration device |
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