Differences in enteric pathogens and intestinal microbiota between diarrheic weaned piglets and healthy penmates
Postweaning diarrhea (PWD) is a multifactorial disease caused by different aetiological agents, like viruses or bacteria and where the role of the microbiota remains unclear. The aim of this study was to assess differences between healthy and diarrheic weaned pigs concerning the prevalence of pathog...
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| Veröffentlicht in: | Veterinary microbiology 2024-08, Vol.295, p.110162, Article 110162 |
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| creator | Garcias, Biel Migura-Garcia, Lourdes Giler, Noemí Martín, Marga Darwich, Laila |
| description | Postweaning diarrhea (PWD) is a multifactorial disease caused by different aetiological agents, like viruses or bacteria and where the role of the microbiota remains unclear. The aim of this study was to assess differences between healthy and diarrheic weaned pigs concerning the prevalence of pathogens and changes in the intestinal microbiota. Eighteen farms with PWD were selected and 277 fecal samples were collected (152 diarrheic vs 125 healthy). Presence of Rotavirus A (RVA), B (RVB), C (RVC) and Porcine Epidemic Diarrhea Virus (PEDV), virulence factors of Escherichia coli and Clostridioides difficile were analyzed by PCR. Finally, the microbiota composition was also study by 16 S rRNA sequencing on 148 samples (102 diarrheic vs 46 healthy). RVA (53.95 % vs 36 %, p=0.04) and RVB (49.67 % vs 28.8 %, p |
| doi_str_mv | 10.1016/j.vetmic.2024.110162 |
| format | Article |
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•Rotavirus A (RVA) was the main pathogen implicated in postweaning diarrhea.•Classical Escherichia coli virulence factors were not linked to diarrhea, indicating the need to update this information.•Ruminococcaceae was more abundant in healthy animals, while Fusobacteriota was more frequent in those with diarrhea.•Lactobacillus was associated with lower prevalence of RVA, indicating a possible protective action.</description><identifier>ISSN: 0378-1135</identifier><identifier>ISSN: 1873-2542</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2024.110162</identifier><identifier>PMID: 38941767</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>16 S ; Animals ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Clostridioides difficile - genetics ; Clostridioides difficile - isolation & purification ; Diarrhea - microbiology ; Diarrhea - veterinary ; Diarrhea - virology ; Escherichia coli ; Escherichia coli - isolation & purification ; Feces - microbiology ; Feces - virology ; Gastrointestinal Microbiome ; Infectious diseases ; Lactobacillus ; Microbiota ; Porcine epidemic diarrhea virus - genetics ; Porcine epidemic diarrhea virus - isolation & purification ; Postweaning Diarrhea ; RNA, Ribosomal, 16S - genetics ; Rotavirus ; Rotavirus - isolation & purification ; Swine ; Swine Diseases - microbiology ; Swine Diseases - virology ; Virulence Factors - genetics ; Weaning</subject><ispartof>Veterinary microbiology, 2024-08, Vol.295, p.110162, Article 110162</ispartof><rights>2024 The Authors</rights><rights>Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c287t-74b7cc9e9bd82d28f89dbd25b27e275ce42b252483dc42eddd6f1dcf396bff373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetmic.2024.110162$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38941767$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garcias, Biel</creatorcontrib><creatorcontrib>Migura-Garcia, Lourdes</creatorcontrib><creatorcontrib>Giler, Noemí</creatorcontrib><creatorcontrib>Martín, Marga</creatorcontrib><creatorcontrib>Darwich, Laila</creatorcontrib><title>Differences in enteric pathogens and intestinal microbiota between diarrheic weaned piglets and healthy penmates</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Postweaning diarrhea (PWD) is a multifactorial disease caused by different aetiological agents, like viruses or bacteria and where the role of the microbiota remains unclear. The aim of this study was to assess differences between healthy and diarrheic weaned pigs concerning the prevalence of pathogens and changes in the intestinal microbiota. Eighteen farms with PWD were selected and 277 fecal samples were collected (152 diarrheic vs 125 healthy). Presence of Rotavirus A (RVA), B (RVB), C (RVC) and Porcine Epidemic Diarrhea Virus (PEDV), virulence factors of Escherichia coli and Clostridioides difficile were analyzed by PCR. Finally, the microbiota composition was also study by 16 S rRNA sequencing on 148 samples (102 diarrheic vs 46 healthy). RVA (53.95 % vs 36 %, p=0.04) and RVB (49.67 % vs 28.8 %, p<0.001) were more frequent in diarrheic animals. Furthermore, RVA viral load was higher in diseased animals. VT2 toxin was significantly associated with diarrhea, whereas other virulence factors were not. Presence of C. difficile and PEDV was almost negligible. Regarding microbiota changes, Fusobacteriota phylum was more frequent in diarrheic samples and Ruminococcaceae family in healthy penmates. During the first week postweaning, Enterobacteriace and Campylobacteria were enriched in animals presenting diarrhea. Furthermore, Lactobacillus was detected in those individuals with no RVA infection. In conclusion, RVA seems to play a primary role in PWD. Classic E. coli virulence factors were not associated with diarrhea, indicating the need for revising their implication in disease. Moreover, Lactobacillus was found frequently in animals negative for RVA, suggesting some protective effect.
•Rotavirus A (RVA) was the main pathogen implicated in postweaning diarrhea.•Classical Escherichia coli virulence factors were not linked to diarrhea, indicating the need to update this information.•Ruminococcaceae was more abundant in healthy animals, while Fusobacteriota was more frequent in those with diarrhea.•Lactobacillus was associated with lower prevalence of RVA, indicating a possible protective action.</description><subject>16 S</subject><subject>Animals</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Clostridioides difficile - genetics</subject><subject>Clostridioides difficile - isolation & purification</subject><subject>Diarrhea - microbiology</subject><subject>Diarrhea - veterinary</subject><subject>Diarrhea - virology</subject><subject>Escherichia coli</subject><subject>Escherichia coli - isolation & purification</subject><subject>Feces - microbiology</subject><subject>Feces - virology</subject><subject>Gastrointestinal Microbiome</subject><subject>Infectious diseases</subject><subject>Lactobacillus</subject><subject>Microbiota</subject><subject>Porcine epidemic diarrhea virus - genetics</subject><subject>Porcine epidemic diarrhea virus - isolation & purification</subject><subject>Postweaning Diarrhea</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Rotavirus</subject><subject>Rotavirus - isolation & purification</subject><subject>Swine</subject><subject>Swine Diseases - microbiology</subject><subject>Swine Diseases - virology</subject><subject>Virulence Factors - genetics</subject><subject>Weaning</subject><issn>0378-1135</issn><issn>1873-2542</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtv1TAQhS0Eorct_wAhL9nk4lfiZIOEWlqQKrGha8uPca-vEifYvq3673GUliWrkWbOmTPzIfSRkj0ltPty3D9CmYLdM8LEnq499gbtaC95w1rB3qId4bJvKOXtGTrP-UgIEUNH3qMz3g-Cyk7u0HIdvIcE0ULGIWKIBVKweNHlMD9AzFhHVwcFcglRj7gmptmEuWhsoDwBROyCTukA1fUEOoLDS3gYoWzWA-ixHJ7xAnHSdcsleuf1mOHDS71A9zfff1_9aO5-3f68-nbXWNbL0khhpLUDDMb1zLHe94MzjrWGSWCytSCYYS0TPXdWMHDOdZ466_nQGe-55Bfo87Z3SfOfU71eTSFbGMd64XzKihPJu5azdpWKTVo_yzmBV0sKk07PihK1clVHtbFWK2u1sa62Ty8JJzOB-2d6hVsFXzcB1D8fAySVbVhJu5DAFuXm8P-Ev9gylNY</recordid><startdate>202408</startdate><enddate>202408</enddate><creator>Garcias, Biel</creator><creator>Migura-Garcia, Lourdes</creator><creator>Giler, Noemí</creator><creator>Martín, Marga</creator><creator>Darwich, Laila</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202408</creationdate><title>Differences in enteric pathogens and intestinal microbiota between diarrheic weaned piglets and healthy penmates</title><author>Garcias, Biel ; Migura-Garcia, Lourdes ; Giler, Noemí ; Martín, Marga ; Darwich, Laila</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c287t-74b7cc9e9bd82d28f89dbd25b27e275ce42b252483dc42eddd6f1dcf396bff373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>16 S</topic><topic>Animals</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Clostridioides difficile - genetics</topic><topic>Clostridioides difficile - isolation & purification</topic><topic>Diarrhea - microbiology</topic><topic>Diarrhea - veterinary</topic><topic>Diarrhea - virology</topic><topic>Escherichia coli</topic><topic>Escherichia coli - isolation & purification</topic><topic>Feces - microbiology</topic><topic>Feces - virology</topic><topic>Gastrointestinal Microbiome</topic><topic>Infectious diseases</topic><topic>Lactobacillus</topic><topic>Microbiota</topic><topic>Porcine epidemic diarrhea virus - genetics</topic><topic>Porcine epidemic diarrhea virus - isolation & purification</topic><topic>Postweaning Diarrhea</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Rotavirus</topic><topic>Rotavirus - isolation & purification</topic><topic>Swine</topic><topic>Swine Diseases - microbiology</topic><topic>Swine Diseases - virology</topic><topic>Virulence Factors - genetics</topic><topic>Weaning</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garcias, Biel</creatorcontrib><creatorcontrib>Migura-Garcia, Lourdes</creatorcontrib><creatorcontrib>Giler, Noemí</creatorcontrib><creatorcontrib>Martín, Marga</creatorcontrib><creatorcontrib>Darwich, Laila</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garcias, Biel</au><au>Migura-Garcia, Lourdes</au><au>Giler, Noemí</au><au>Martín, Marga</au><au>Darwich, Laila</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in enteric pathogens and intestinal microbiota between diarrheic weaned piglets and healthy penmates</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2024-08</date><risdate>2024</risdate><volume>295</volume><spage>110162</spage><pages>110162-</pages><artnum>110162</artnum><issn>0378-1135</issn><issn>1873-2542</issn><eissn>1873-2542</eissn><abstract>Postweaning diarrhea (PWD) is a multifactorial disease caused by different aetiological agents, like viruses or bacteria and where the role of the microbiota remains unclear. The aim of this study was to assess differences between healthy and diarrheic weaned pigs concerning the prevalence of pathogens and changes in the intestinal microbiota. Eighteen farms with PWD were selected and 277 fecal samples were collected (152 diarrheic vs 125 healthy). Presence of Rotavirus A (RVA), B (RVB), C (RVC) and Porcine Epidemic Diarrhea Virus (PEDV), virulence factors of Escherichia coli and Clostridioides difficile were analyzed by PCR. Finally, the microbiota composition was also study by 16 S rRNA sequencing on 148 samples (102 diarrheic vs 46 healthy). RVA (53.95 % vs 36 %, p=0.04) and RVB (49.67 % vs 28.8 %, p<0.001) were more frequent in diarrheic animals. Furthermore, RVA viral load was higher in diseased animals. VT2 toxin was significantly associated with diarrhea, whereas other virulence factors were not. Presence of C. difficile and PEDV was almost negligible. Regarding microbiota changes, Fusobacteriota phylum was more frequent in diarrheic samples and Ruminococcaceae family in healthy penmates. During the first week postweaning, Enterobacteriace and Campylobacteria were enriched in animals presenting diarrhea. Furthermore, Lactobacillus was detected in those individuals with no RVA infection. In conclusion, RVA seems to play a primary role in PWD. Classic E. coli virulence factors were not associated with diarrhea, indicating the need for revising their implication in disease. Moreover, Lactobacillus was found frequently in animals negative for RVA, suggesting some protective effect.
•Rotavirus A (RVA) was the main pathogen implicated in postweaning diarrhea.•Classical Escherichia coli virulence factors were not linked to diarrhea, indicating the need to update this information.•Ruminococcaceae was more abundant in healthy animals, while Fusobacteriota was more frequent in those with diarrhea.•Lactobacillus was associated with lower prevalence of RVA, indicating a possible protective action.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>38941767</pmid><doi>10.1016/j.vetmic.2024.110162</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 16 S Animals Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Clostridioides difficile - genetics Clostridioides difficile - isolation & purification Diarrhea - microbiology Diarrhea - veterinary Diarrhea - virology Escherichia coli Escherichia coli - isolation & purification Feces - microbiology Feces - virology Gastrointestinal Microbiome Infectious diseases Lactobacillus Microbiota Porcine epidemic diarrhea virus - genetics Porcine epidemic diarrhea virus - isolation & purification Postweaning Diarrhea RNA, Ribosomal, 16S - genetics Rotavirus Rotavirus - isolation & purification Swine Swine Diseases - microbiology Swine Diseases - virology Virulence Factors - genetics Weaning |
| title | Differences in enteric pathogens and intestinal microbiota between diarrheic weaned piglets and healthy penmates |
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