Microfluidic preparation and optimization of (Kollicoat ® IR-b-PCL) polymersome for co-delivery of Nisin-Curcumin in breast cancer application
[Display omitted] This work aimed to develop amphiphilic nanocarriers such as polymersome based diblock copolymer of Kollicoat ® IR −block-poly(ε-caprolactone) (Kollicoat ® IR-b-PCL) for potential co-delivery of Nisin (Ni) and Curcumin (CUR) for treatment of breast cancer. To generate multi-layered...
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| Veröffentlicht in: | International journal of pharmaceutics 2024-07, Vol.660, p.124371, Article 124371 |
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| container_title | International journal of pharmaceutics |
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| creator | Salehi, Sahar Boddohi, Soheil Adel Ghiass, Mohammad Behmanesh, Mehrdad |
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This work aimed to develop amphiphilic nanocarriers such as polymersome based diblock copolymer of Kollicoat ® IR −block-poly(ε-caprolactone) (Kollicoat ® IR-b-PCL) for potential co-delivery of Nisin (Ni) and Curcumin (CUR) for treatment of breast cancer. To generate multi-layered nanocarriers of uniform size and morphology, microfluidics was used as a new technology. In order to characterise and optimize polymersome, design of experiments (Design-Expert) software with three levels full factorial design (3-FFD) method was used. Finally, the optimized polymersome was produced with a spherical morphology, small particle size (dH |
| doi_str_mv | 10.1016/j.ijpharm.2024.124371 |
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This work aimed to develop amphiphilic nanocarriers such as polymersome based diblock copolymer of Kollicoat ® IR −block-poly(ε-caprolactone) (Kollicoat ® IR-b-PCL) for potential co-delivery of Nisin (Ni) and Curcumin (CUR) for treatment of breast cancer. To generate multi-layered nanocarriers of uniform size and morphology, microfluidics was used as a new technology. In order to characterise and optimize polymersome, design of experiments (Design-Expert) software with three levels full factorial design (3-FFD) method was used. Finally, the optimized polymersome was produced with a spherical morphology, small particle size (dH < 200 nm), uniform size distribution (PDI < 0.2), and high drug loading efficiency (Ni 78 % and CUR 93 %). Furthermore, the maximum release of Ni and CUR was found to be roughly 60 % and 80 % in PBS, respectively. Cytotoxicity assays showed a slight cytotoxicity of Ni and CUR −loaded polymersome (N- Ni /CUR) towards normal cells while demonstrating inhibitory activity against cancer cells compared to the free drugs. Also, the apoptosis assays and cellular uptake confirmed the obtained results from cytotoxic analysis. In general, this study demonstrated a microfluidic approach for preparation and optimization of polymersome for co-delivery of two drugs into cancer cells.</description><identifier>ISSN: 0378-5173</identifier><identifier>ISSN: 1873-3476</identifier><identifier>EISSN: 1873-3476</identifier><identifier>DOI: 10.1016/j.ijpharm.2024.124371</identifier><identifier>PMID: 38908809</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Antineoplastic Agents - administration & dosage ; Antineoplastic Agents - chemistry ; Antineoplastic Agents - pharmacokinetics ; Antineoplastic Agents - pharmacology ; Apoptosis - drug effects ; Breast cancer ; Breast Neoplasms - drug therapy ; Cell Line, Tumor ; Cell Survival - drug effects ; Curcumin ; Curcumin - administration & dosage ; Curcumin - chemistry ; Curcumin - pharmacokinetics ; Curcumin - pharmacology ; Drug Carriers - chemistry ; Drug Delivery Systems ; Drug Liberation ; Female ; Full factorial design ; Humans ; MCF-7 Cells ; Microfluidics ; Microfluidics - methods ; Nisin ; Nisin - administration & dosage ; Nisin - chemistry ; Nisin - pharmacology ; Particle Size ; Polyesters - chemistry ; Polymersome ; Polyvinyls - chemistry</subject><ispartof>International journal of pharmaceutics, 2024-07, Vol.660, p.124371, Article 124371</ispartof><rights>2024</rights><rights>Copyright © 2024. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c243t-6c0c75eebbf71f4434d5edad7326ffee595b540b9d6c71e7ddcb07ed3c0fcd2b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378517324006057$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38908809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salehi, Sahar</creatorcontrib><creatorcontrib>Boddohi, Soheil</creatorcontrib><creatorcontrib>Adel Ghiass, Mohammad</creatorcontrib><creatorcontrib>Behmanesh, Mehrdad</creatorcontrib><title>Microfluidic preparation and optimization of (Kollicoat ® IR-b-PCL) polymersome for co-delivery of Nisin-Curcumin in breast cancer application</title><title>International journal of pharmaceutics</title><addtitle>Int J Pharm</addtitle><description>[Display omitted]
This work aimed to develop amphiphilic nanocarriers such as polymersome based diblock copolymer of Kollicoat ® IR −block-poly(ε-caprolactone) (Kollicoat ® IR-b-PCL) for potential co-delivery of Nisin (Ni) and Curcumin (CUR) for treatment of breast cancer. To generate multi-layered nanocarriers of uniform size and morphology, microfluidics was used as a new technology. In order to characterise and optimize polymersome, design of experiments (Design-Expert) software with three levels full factorial design (3-FFD) method was used. Finally, the optimized polymersome was produced with a spherical morphology, small particle size (dH < 200 nm), uniform size distribution (PDI < 0.2), and high drug loading efficiency (Ni 78 % and CUR 93 %). Furthermore, the maximum release of Ni and CUR was found to be roughly 60 % and 80 % in PBS, respectively. Cytotoxicity assays showed a slight cytotoxicity of Ni and CUR −loaded polymersome (N- Ni /CUR) towards normal cells while demonstrating inhibitory activity against cancer cells compared to the free drugs. Also, the apoptosis assays and cellular uptake confirmed the obtained results from cytotoxic analysis. In general, this study demonstrated a microfluidic approach for preparation and optimization of polymersome for co-delivery of two drugs into cancer cells.</description><subject>Antineoplastic Agents - administration & dosage</subject><subject>Antineoplastic Agents - chemistry</subject><subject>Antineoplastic Agents - pharmacokinetics</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - drug therapy</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Curcumin</subject><subject>Curcumin - administration & dosage</subject><subject>Curcumin - chemistry</subject><subject>Curcumin - pharmacokinetics</subject><subject>Curcumin - pharmacology</subject><subject>Drug Carriers - chemistry</subject><subject>Drug Delivery Systems</subject><subject>Drug Liberation</subject><subject>Female</subject><subject>Full factorial design</subject><subject>Humans</subject><subject>MCF-7 Cells</subject><subject>Microfluidics</subject><subject>Microfluidics - methods</subject><subject>Nisin</subject><subject>Nisin - administration & dosage</subject><subject>Nisin - chemistry</subject><subject>Nisin - pharmacology</subject><subject>Particle Size</subject><subject>Polyesters - chemistry</subject><subject>Polymersome</subject><subject>Polyvinyls - chemistry</subject><issn>0378-5173</issn><issn>1873-3476</issn><issn>1873-3476</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUduKFDEUDOLizq5-gpLH9SGzSae70_MkMui6OF4QfQ65nGCG7k5Muhdmf2L_xI_wy8zYo6_CgQOHqlNUFULPGV0zytrr_drv43eVhnVFq3rNqpoL9gitWCc44bVoH6MV5aIjDRP8HF3kvKeUthXjT9A57za06-hmhR4-eJOC62dvvcExQVRJTT6MWI0Whzj5wd8vh-Dw1fvQ994ENeFfP_HtF6LJ5-3uJY6hPwyQchgAu5CwCcRC7-8gHY60jz77kWznZObBj7iMTqDyhI0aDSSsYixf_6g8RWdO9RmenfYl-vb2zdftO7L7dHO7fb0jphidSGuoEQ2A1k4wV9e8tg1YZQWvWucAmk2jm5rqjW2NYCCsNZoKsNxQZ2yl-SW6Wv7GFH7MkCc5-Gyg79UIYc6SU8GqruRYF2izQEtQOSdwMiY_qHSQjMpjF3IvT13IYxdy6aLwXpwkZj2A_cf6G34BvFoAUIzeeUgyGw8lEesTmEna4P8j8RvkiKGI</recordid><startdate>20240720</startdate><enddate>20240720</enddate><creator>Salehi, Sahar</creator><creator>Boddohi, Soheil</creator><creator>Adel Ghiass, Mohammad</creator><creator>Behmanesh, Mehrdad</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20240720</creationdate><title>Microfluidic preparation and optimization of (Kollicoat ® IR-b-PCL) polymersome for co-delivery of Nisin-Curcumin in breast cancer application</title><author>Salehi, Sahar ; Boddohi, Soheil ; Adel Ghiass, Mohammad ; Behmanesh, Mehrdad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c243t-6c0c75eebbf71f4434d5edad7326ffee595b540b9d6c71e7ddcb07ed3c0fcd2b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antineoplastic Agents - administration & dosage</topic><topic>Antineoplastic Agents - chemistry</topic><topic>Antineoplastic Agents - pharmacokinetics</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - drug therapy</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Curcumin</topic><topic>Curcumin - administration & dosage</topic><topic>Curcumin - chemistry</topic><topic>Curcumin - pharmacokinetics</topic><topic>Curcumin - pharmacology</topic><topic>Drug Carriers - chemistry</topic><topic>Drug Delivery Systems</topic><topic>Drug Liberation</topic><topic>Female</topic><topic>Full factorial design</topic><topic>Humans</topic><topic>MCF-7 Cells</topic><topic>Microfluidics</topic><topic>Microfluidics - methods</topic><topic>Nisin</topic><topic>Nisin - administration & dosage</topic><topic>Nisin - chemistry</topic><topic>Nisin - pharmacology</topic><topic>Particle Size</topic><topic>Polyesters - chemistry</topic><topic>Polymersome</topic><topic>Polyvinyls - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salehi, Sahar</creatorcontrib><creatorcontrib>Boddohi, Soheil</creatorcontrib><creatorcontrib>Adel Ghiass, Mohammad</creatorcontrib><creatorcontrib>Behmanesh, Mehrdad</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of pharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salehi, Sahar</au><au>Boddohi, Soheil</au><au>Adel Ghiass, Mohammad</au><au>Behmanesh, Mehrdad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microfluidic preparation and optimization of (Kollicoat ® IR-b-PCL) polymersome for co-delivery of Nisin-Curcumin in breast cancer application</atitle><jtitle>International journal of pharmaceutics</jtitle><addtitle>Int J Pharm</addtitle><date>2024-07-20</date><risdate>2024</risdate><volume>660</volume><spage>124371</spage><pages>124371-</pages><artnum>124371</artnum><issn>0378-5173</issn><issn>1873-3476</issn><eissn>1873-3476</eissn><abstract>[Display omitted]
This work aimed to develop amphiphilic nanocarriers such as polymersome based diblock copolymer of Kollicoat ® IR −block-poly(ε-caprolactone) (Kollicoat ® IR-b-PCL) for potential co-delivery of Nisin (Ni) and Curcumin (CUR) for treatment of breast cancer. To generate multi-layered nanocarriers of uniform size and morphology, microfluidics was used as a new technology. In order to characterise and optimize polymersome, design of experiments (Design-Expert) software with three levels full factorial design (3-FFD) method was used. Finally, the optimized polymersome was produced with a spherical morphology, small particle size (dH < 200 nm), uniform size distribution (PDI < 0.2), and high drug loading efficiency (Ni 78 % and CUR 93 %). Furthermore, the maximum release of Ni and CUR was found to be roughly 60 % and 80 % in PBS, respectively. Cytotoxicity assays showed a slight cytotoxicity of Ni and CUR −loaded polymersome (N- Ni /CUR) towards normal cells while demonstrating inhibitory activity against cancer cells compared to the free drugs. Also, the apoptosis assays and cellular uptake confirmed the obtained results from cytotoxic analysis. In general, this study demonstrated a microfluidic approach for preparation and optimization of polymersome for co-delivery of two drugs into cancer cells.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>38908809</pmid><doi>10.1016/j.ijpharm.2024.124371</doi></addata></record> |
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| subjects | Antineoplastic Agents - administration & dosage Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacokinetics Antineoplastic Agents - pharmacology Apoptosis - drug effects Breast cancer Breast Neoplasms - drug therapy Cell Line, Tumor Cell Survival - drug effects Curcumin Curcumin - administration & dosage Curcumin - chemistry Curcumin - pharmacokinetics Curcumin - pharmacology Drug Carriers - chemistry Drug Delivery Systems Drug Liberation Female Full factorial design Humans MCF-7 Cells Microfluidics Microfluidics - methods Nisin Nisin - administration & dosage Nisin - chemistry Nisin - pharmacology Particle Size Polyesters - chemistry Polymersome Polyvinyls - chemistry |
| title | Microfluidic preparation and optimization of (Kollicoat ® IR-b-PCL) polymersome for co-delivery of Nisin-Curcumin in breast cancer application |
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