Evaluation of effects of Tempol on testicular ischemia/reperfusion injury
Tempol, a synthetic antioxidant compound, has received significant attention for its potential therapeutic applications in recent years, especially against ischemia/reperfusion (I/R) injury. The aim of the present research was to assess the protective effects of Tempol on testicular I/R injury cause...
Gespeichert in:
| Veröffentlicht in: | The American journal of emergency medicine 2024-08, Vol.82, p.107-116 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 116 |
|---|---|
| container_issue | |
| container_start_page | 107 |
| container_title | The American journal of emergency medicine |
| container_volume | 82 |
| creator | Ganjiani, Vahid Meimandi-Parizi, Abdolhamid Ahmadi, Nasrollah Sharifiyazdi, Hassan Divar, Mohammad-Reza |
| description | Tempol, a synthetic antioxidant compound, has received significant attention for its potential therapeutic applications in recent years, especially against ischemia/reperfusion (I/R) injury. The aim of the present research was to assess the protective effects of Tempol on testicular I/R injury caused by testicular torsion and detorsion (T/D) in rats.
The subjects were divided into five groups: sham, testicular T/D, testicular T/D with Tempol treatment at 50 and 100 mg/kg, and healthy rats treated with Tempol at 100 mg/kg. Testicular torsion was induced by rotating the left testicles for 2 h, followed by detorsion for 24 h. Testicular tissues were evaluated for gene expression, oxidative stress markers, and histopathology, epididymal sperms were stained and analyzed, and blood serum samples were collected to measure the testosterone hormone.
The results showed that testicular I/R caused a significant decrease in sperm velocity parameters, viability, and count, as well as an increase in abnormal sperms (p |
| doi_str_mv | 10.1016/j.ajem.2024.06.009 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3070838393</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0735675724002742</els_id><sourcerecordid>3070838393</sourcerecordid><originalsourceid>FETCH-LOGICAL-c265t-449b021a1f4d3dcc52d7c462308c1c51d43b1d2a9981775be24e65114cc84ddd3</originalsourceid><addsrcrecordid>eNp9kMtKAzEUhoMoWqsv4EIKbtzMNCeXuYAbKfUCghtdhzQ5gxnmUpOZQt_eDFUXLlwlhO8_-c9HyBXQFChkyzrVNbYpo0ykNEspLY_IDCRnSQE5HJMZzblMslzmZ-Q8hJpSACHFKTnjRUmBc5iR5_VON6MeXN8t-mqBVYVmCNP1Ddtt3yzi-4BhcGZstF-4YD6wdXrpcYu-GsOUc109-v0FOal0E_Dy-5yT94f12-opeXl9fF7dvySGZXJIhCg3lIGGSlhujZHM5kZkjNPCgJFgBd-AZbos4xK53CATmMlY3JhCWGv5nNwe5m59_znGaqqNrbBpdIf9GBSnOS14wUse0Zs_aN2PvovtIlUAAI__RoodKOP7EDxWautdq_1eAVWTaFWrSbSaRCuaqSg6hq6_R4-bFu1v5MdsBO4OAEYXO4deBeOwM2idj4qV7d1_878AtFeN2w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3081113623</pqid></control><display><type>article</type><title>Evaluation of effects of Tempol on testicular ischemia/reperfusion injury</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>ProQuest Central UK/Ireland</source><creator>Ganjiani, Vahid ; Meimandi-Parizi, Abdolhamid ; Ahmadi, Nasrollah ; Sharifiyazdi, Hassan ; Divar, Mohammad-Reza</creator><creatorcontrib>Ganjiani, Vahid ; Meimandi-Parizi, Abdolhamid ; Ahmadi, Nasrollah ; Sharifiyazdi, Hassan ; Divar, Mohammad-Reza</creatorcontrib><description><![CDATA[Tempol, a synthetic antioxidant compound, has received significant attention for its potential therapeutic applications in recent years, especially against ischemia/reperfusion (I/R) injury. The aim of the present research was to assess the protective effects of Tempol on testicular I/R injury caused by testicular torsion and detorsion (T/D) in rats.
The subjects were divided into five groups: sham, testicular T/D, testicular T/D with Tempol treatment at 50 and 100 mg/kg, and healthy rats treated with Tempol at 100 mg/kg. Testicular torsion was induced by rotating the left testicles for 2 h, followed by detorsion for 24 h. Testicular tissues were evaluated for gene expression, oxidative stress markers, and histopathology, epididymal sperms were stained and analyzed, and blood serum samples were collected to measure the testosterone hormone.
The results showed that testicular I/R caused a significant decrease in sperm velocity parameters, viability, and count, as well as an increase in abnormal sperms (p < 0.05). However, treatment with Tempol significantly improved these parameters (p < 0.05). Histopathological analysis revealed severe damage to the testicular tissues, but treatment with Tempol improved the structural integrity of the seminiferous tubules. Testicular I/R also resulted in increased oxidative stress index and decreased testosterone levels significantly (p < 0.05), but Tempol administration mitigated these effects significantly (p < 0.05). Furthermore, the expression of Bax and Bcl2, genes associated with apoptosis, were significantly altered by testicular I/R (p < 0.05), but Tempol prevented these changes significantly (p < 0.05).
These findings provide strong evidence that Tempol can effectively prevent testicular I/R injury.]]></description><identifier>ISSN: 0735-6757</identifier><identifier>ISSN: 1532-8171</identifier><identifier>EISSN: 1532-8171</identifier><identifier>DOI: 10.1016/j.ajem.2024.06.009</identifier><identifier>PMID: 38901331</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antioxidants ; Antioxidants - pharmacology ; Antioxidants - therapeutic use ; Apoptosis ; BAX protein ; Bcl-2 protein ; Biomarkers ; Cyclic N-Oxides - pharmacology ; Cyclic N-Oxides - therapeutic use ; Disease Models, Animal ; Gene expression ; Histopathology ; Ischemia ; ischemia/reperfusion injury ; Male ; Oxidative stress ; Oxidative Stress - drug effects ; Rats ; Rats, Sprague-Dawley ; Reperfusion ; Reperfusion Injury - prevention & control ; Sperm ; Spermatic Cord Torsion - complications ; Spermatic Cord Torsion - drug therapy ; Spin Labels ; Stains & staining ; Tempol ; Testes ; Testis - blood supply ; Testis - drug effects ; Testis - pathology ; Testosterone ; Therapeutic applications ; Torsion ; Tubules</subject><ispartof>The American journal of emergency medicine, 2024-08, Vol.82, p.107-116</ispartof><rights>2024</rights><rights>Copyright © 2024. Published by Elsevier Inc.</rights><rights>Copyright Elsevier Limited Aug 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c265t-449b021a1f4d3dcc52d7c462308c1c51d43b1d2a9981775be24e65114cc84ddd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/3081113623?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,64361,64363,64365,65309,72215</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38901331$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ganjiani, Vahid</creatorcontrib><creatorcontrib>Meimandi-Parizi, Abdolhamid</creatorcontrib><creatorcontrib>Ahmadi, Nasrollah</creatorcontrib><creatorcontrib>Sharifiyazdi, Hassan</creatorcontrib><creatorcontrib>Divar, Mohammad-Reza</creatorcontrib><title>Evaluation of effects of Tempol on testicular ischemia/reperfusion injury</title><title>The American journal of emergency medicine</title><addtitle>Am J Emerg Med</addtitle><description><![CDATA[Tempol, a synthetic antioxidant compound, has received significant attention for its potential therapeutic applications in recent years, especially against ischemia/reperfusion (I/R) injury. The aim of the present research was to assess the protective effects of Tempol on testicular I/R injury caused by testicular torsion and detorsion (T/D) in rats.
The subjects were divided into five groups: sham, testicular T/D, testicular T/D with Tempol treatment at 50 and 100 mg/kg, and healthy rats treated with Tempol at 100 mg/kg. Testicular torsion was induced by rotating the left testicles for 2 h, followed by detorsion for 24 h. Testicular tissues were evaluated for gene expression, oxidative stress markers, and histopathology, epididymal sperms were stained and analyzed, and blood serum samples were collected to measure the testosterone hormone.
The results showed that testicular I/R caused a significant decrease in sperm velocity parameters, viability, and count, as well as an increase in abnormal sperms (p < 0.05). However, treatment with Tempol significantly improved these parameters (p < 0.05). Histopathological analysis revealed severe damage to the testicular tissues, but treatment with Tempol improved the structural integrity of the seminiferous tubules. Testicular I/R also resulted in increased oxidative stress index and decreased testosterone levels significantly (p < 0.05), but Tempol administration mitigated these effects significantly (p < 0.05). Furthermore, the expression of Bax and Bcl2, genes associated with apoptosis, were significantly altered by testicular I/R (p < 0.05), but Tempol prevented these changes significantly (p < 0.05).
These findings provide strong evidence that Tempol can effectively prevent testicular I/R injury.]]></description><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Antioxidants - therapeutic use</subject><subject>Apoptosis</subject><subject>BAX protein</subject><subject>Bcl-2 protein</subject><subject>Biomarkers</subject><subject>Cyclic N-Oxides - pharmacology</subject><subject>Cyclic N-Oxides - therapeutic use</subject><subject>Disease Models, Animal</subject><subject>Gene expression</subject><subject>Histopathology</subject><subject>Ischemia</subject><subject>ischemia/reperfusion injury</subject><subject>Male</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Sperm</subject><subject>Spermatic Cord Torsion - complications</subject><subject>Spermatic Cord Torsion - drug therapy</subject><subject>Spin Labels</subject><subject>Stains & staining</subject><subject>Tempol</subject><subject>Testes</subject><subject>Testis - blood supply</subject><subject>Testis - drug effects</subject><subject>Testis - pathology</subject><subject>Testosterone</subject><subject>Therapeutic applications</subject><subject>Torsion</subject><subject>Tubules</subject><issn>0735-6757</issn><issn>1532-8171</issn><issn>1532-8171</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kMtKAzEUhoMoWqsv4EIKbtzMNCeXuYAbKfUCghtdhzQ5gxnmUpOZQt_eDFUXLlwlhO8_-c9HyBXQFChkyzrVNbYpo0ykNEspLY_IDCRnSQE5HJMZzblMslzmZ-Q8hJpSACHFKTnjRUmBc5iR5_VON6MeXN8t-mqBVYVmCNP1Ddtt3yzi-4BhcGZstF-4YD6wdXrpcYu-GsOUc109-v0FOal0E_Dy-5yT94f12-opeXl9fF7dvySGZXJIhCg3lIGGSlhujZHM5kZkjNPCgJFgBd-AZbos4xK53CATmMlY3JhCWGv5nNwe5m59_znGaqqNrbBpdIf9GBSnOS14wUse0Zs_aN2PvovtIlUAAI__RoodKOP7EDxWautdq_1eAVWTaFWrSbSaRCuaqSg6hq6_R4-bFu1v5MdsBO4OAEYXO4deBeOwM2idj4qV7d1_878AtFeN2w</recordid><startdate>202408</startdate><enddate>202408</enddate><creator>Ganjiani, Vahid</creator><creator>Meimandi-Parizi, Abdolhamid</creator><creator>Ahmadi, Nasrollah</creator><creator>Sharifiyazdi, Hassan</creator><creator>Divar, Mohammad-Reza</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>202408</creationdate><title>Evaluation of effects of Tempol on testicular ischemia/reperfusion injury</title><author>Ganjiani, Vahid ; Meimandi-Parizi, Abdolhamid ; Ahmadi, Nasrollah ; Sharifiyazdi, Hassan ; Divar, Mohammad-Reza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c265t-449b021a1f4d3dcc52d7c462308c1c51d43b1d2a9981775be24e65114cc84ddd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Antioxidants - therapeutic use</topic><topic>Apoptosis</topic><topic>BAX protein</topic><topic>Bcl-2 protein</topic><topic>Biomarkers</topic><topic>Cyclic N-Oxides - pharmacology</topic><topic>Cyclic N-Oxides - therapeutic use</topic><topic>Disease Models, Animal</topic><topic>Gene expression</topic><topic>Histopathology</topic><topic>Ischemia</topic><topic>ischemia/reperfusion injury</topic><topic>Male</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion</topic><topic>Reperfusion Injury - prevention & control</topic><topic>Sperm</topic><topic>Spermatic Cord Torsion - complications</topic><topic>Spermatic Cord Torsion - drug therapy</topic><topic>Spin Labels</topic><topic>Stains & staining</topic><topic>Tempol</topic><topic>Testes</topic><topic>Testis - blood supply</topic><topic>Testis - drug effects</topic><topic>Testis - pathology</topic><topic>Testosterone</topic><topic>Therapeutic applications</topic><topic>Torsion</topic><topic>Tubules</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ganjiani, Vahid</creatorcontrib><creatorcontrib>Meimandi-Parizi, Abdolhamid</creatorcontrib><creatorcontrib>Ahmadi, Nasrollah</creatorcontrib><creatorcontrib>Sharifiyazdi, Hassan</creatorcontrib><creatorcontrib>Divar, Mohammad-Reza</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of emergency medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ganjiani, Vahid</au><au>Meimandi-Parizi, Abdolhamid</au><au>Ahmadi, Nasrollah</au><au>Sharifiyazdi, Hassan</au><au>Divar, Mohammad-Reza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of effects of Tempol on testicular ischemia/reperfusion injury</atitle><jtitle>The American journal of emergency medicine</jtitle><addtitle>Am J Emerg Med</addtitle><date>2024-08</date><risdate>2024</risdate><volume>82</volume><spage>107</spage><epage>116</epage><pages>107-116</pages><issn>0735-6757</issn><issn>1532-8171</issn><eissn>1532-8171</eissn><abstract><![CDATA[Tempol, a synthetic antioxidant compound, has received significant attention for its potential therapeutic applications in recent years, especially against ischemia/reperfusion (I/R) injury. The aim of the present research was to assess the protective effects of Tempol on testicular I/R injury caused by testicular torsion and detorsion (T/D) in rats.
The subjects were divided into five groups: sham, testicular T/D, testicular T/D with Tempol treatment at 50 and 100 mg/kg, and healthy rats treated with Tempol at 100 mg/kg. Testicular torsion was induced by rotating the left testicles for 2 h, followed by detorsion for 24 h. Testicular tissues were evaluated for gene expression, oxidative stress markers, and histopathology, epididymal sperms were stained and analyzed, and blood serum samples were collected to measure the testosterone hormone.
The results showed that testicular I/R caused a significant decrease in sperm velocity parameters, viability, and count, as well as an increase in abnormal sperms (p < 0.05). However, treatment with Tempol significantly improved these parameters (p < 0.05). Histopathological analysis revealed severe damage to the testicular tissues, but treatment with Tempol improved the structural integrity of the seminiferous tubules. Testicular I/R also resulted in increased oxidative stress index and decreased testosterone levels significantly (p < 0.05), but Tempol administration mitigated these effects significantly (p < 0.05). Furthermore, the expression of Bax and Bcl2, genes associated with apoptosis, were significantly altered by testicular I/R (p < 0.05), but Tempol prevented these changes significantly (p < 0.05).
These findings provide strong evidence that Tempol can effectively prevent testicular I/R injury.]]></abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>38901331</pmid><doi>10.1016/j.ajem.2024.06.009</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0735-6757 |
| ispartof | The American journal of emergency medicine, 2024-08, Vol.82, p.107-116 |
| issn | 0735-6757 1532-8171 1532-8171 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3070838393 |
| source | MEDLINE; Elsevier ScienceDirect Journals; ProQuest Central UK/Ireland |
| subjects | Animals Antioxidants Antioxidants - pharmacology Antioxidants - therapeutic use Apoptosis BAX protein Bcl-2 protein Biomarkers Cyclic N-Oxides - pharmacology Cyclic N-Oxides - therapeutic use Disease Models, Animal Gene expression Histopathology Ischemia ischemia/reperfusion injury Male Oxidative stress Oxidative Stress - drug effects Rats Rats, Sprague-Dawley Reperfusion Reperfusion Injury - prevention & control Sperm Spermatic Cord Torsion - complications Spermatic Cord Torsion - drug therapy Spin Labels Stains & staining Tempol Testes Testis - blood supply Testis - drug effects Testis - pathology Testosterone Therapeutic applications Torsion Tubules |
| title | Evaluation of effects of Tempol on testicular ischemia/reperfusion injury |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T07%3A28%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20effects%20of%20Tempol%20on%20testicular%20ischemia/reperfusion%20injury&rft.jtitle=The%20American%20journal%20of%20emergency%20medicine&rft.au=Ganjiani,%20Vahid&rft.date=2024-08&rft.volume=82&rft.spage=107&rft.epage=116&rft.pages=107-116&rft.issn=0735-6757&rft.eissn=1532-8171&rft_id=info:doi/10.1016/j.ajem.2024.06.009&rft_dat=%3Cproquest_cross%3E3070838393%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3081113623&rft_id=info:pmid/38901331&rft_els_id=S0735675724002742&rfr_iscdi=true |