Four-Dimensional Label-Free Quantitative Proteomics of Ginsenoside Rg2 Ameliorated Scopolamine-Induced Memory Impairment in Mice through the Lysosomal Pathway
Alzheimer’s disease (AD) is a neurodegenerative disease. Ginsenoside Rg2 has shown potential in treating AD, but the underlying protein regulatory mechanisms associated with ginsenoside Rg2 treatment for AD remain unclear. This study utilized scopolamine to induce memory impairment in mice, and prot...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2024-07, Vol.72 (26), p.14640-14652 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Yin, Pei Wang, Heyu Xue, Tingfang Yu, Xiaoran Meng, Xingjian Mi, Qianwen Song, Shixin Xiong, Boyu Bi, Yunfeng Yu, Lei |
| description | Alzheimer’s disease (AD) is a neurodegenerative disease. Ginsenoside Rg2 has shown potential in treating AD, but the underlying protein regulatory mechanisms associated with ginsenoside Rg2 treatment for AD remain unclear. This study utilized scopolamine to induce memory impairment in mice, and proteomics methods were employed to investigate the potential molecular mechanism of ginsenoside Rg2 in treating AD model mice. The Morris water maze, hematoxylin and eosin staining, and Nissl staining results indicated that ginsenoside Rg2 enhanced cognitive ability and decreased neuronal damage in AD mice. Proteomics, western blot, and immunofluorescence results showed that ginsenoside Rg2 primarily improved AD mice by downregulating the expression of LGMN, LAMP1, and PSAP proteins through the regulation of the lysosomal pathway. Transmission electron microscopy and network pharmacology prediction results showed a potential connection between the mechanism of ginsenoside Rg2 treatment for AD mice and lysosomes. The comprehensive results indicated that ginsenoside Rg2 may improve AD by downregulating LGMN, LAMP1, and PSAP through the regulation of the lysosomal pathway. |
| doi_str_mv | 10.1021/acs.jafc.4c00181 |
| format | Article |
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Ginsenoside Rg2 has shown potential in treating AD, but the underlying protein regulatory mechanisms associated with ginsenoside Rg2 treatment for AD remain unclear. This study utilized scopolamine to induce memory impairment in mice, and proteomics methods were employed to investigate the potential molecular mechanism of ginsenoside Rg2 in treating AD model mice. The Morris water maze, hematoxylin and eosin staining, and Nissl staining results indicated that ginsenoside Rg2 enhanced cognitive ability and decreased neuronal damage in AD mice. Proteomics, western blot, and immunofluorescence results showed that ginsenoside Rg2 primarily improved AD mice by downregulating the expression of LGMN, LAMP1, and PSAP proteins through the regulation of the lysosomal pathway. Transmission electron microscopy and network pharmacology prediction results showed a potential connection between the mechanism of ginsenoside Rg2 treatment for AD mice and lysosomes. The comprehensive results indicated that ginsenoside Rg2 may improve AD by downregulating LGMN, LAMP1, and PSAP through the regulation of the lysosomal pathway.</description><identifier>ISSN: 0021-8561</identifier><identifier>ISSN: 1520-5118</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.4c00181</identifier><language>eng</language><publisher>American Chemical Society</publisher><subject>Bioactive Constituents, Metabolites, and Functions ; cognition ; eosin ; fluorescent antibody technique ; food chemistry ; ginsenosides ; lysosomes ; memory disorders ; neurodegenerative diseases ; neurons ; pharmacology ; prediction ; proteomics ; scopolamine ; transmission electron microscopy ; Western blotting</subject><ispartof>Journal of agricultural and food chemistry, 2024-07, Vol.72 (26), p.14640-14652</ispartof><rights>2024 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0009-0006-4166-2424 ; 0000-0002-6934-1319</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.4c00181$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.4c00181$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,777,781,27057,27905,27906,56719,56769</link.rule.ids></links><search><creatorcontrib>Yin, Pei</creatorcontrib><creatorcontrib>Wang, Heyu</creatorcontrib><creatorcontrib>Xue, Tingfang</creatorcontrib><creatorcontrib>Yu, Xiaoran</creatorcontrib><creatorcontrib>Meng, Xingjian</creatorcontrib><creatorcontrib>Mi, Qianwen</creatorcontrib><creatorcontrib>Song, Shixin</creatorcontrib><creatorcontrib>Xiong, Boyu</creatorcontrib><creatorcontrib>Bi, Yunfeng</creatorcontrib><creatorcontrib>Yu, Lei</creatorcontrib><title>Four-Dimensional Label-Free Quantitative Proteomics of Ginsenoside Rg2 Ameliorated Scopolamine-Induced Memory Impairment in Mice through the Lysosomal Pathway</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Alzheimer’s disease (AD) is a neurodegenerative disease. Ginsenoside Rg2 has shown potential in treating AD, but the underlying protein regulatory mechanisms associated with ginsenoside Rg2 treatment for AD remain unclear. This study utilized scopolamine to induce memory impairment in mice, and proteomics methods were employed to investigate the potential molecular mechanism of ginsenoside Rg2 in treating AD model mice. The Morris water maze, hematoxylin and eosin staining, and Nissl staining results indicated that ginsenoside Rg2 enhanced cognitive ability and decreased neuronal damage in AD mice. Proteomics, western blot, and immunofluorescence results showed that ginsenoside Rg2 primarily improved AD mice by downregulating the expression of LGMN, LAMP1, and PSAP proteins through the regulation of the lysosomal pathway. Transmission electron microscopy and network pharmacology prediction results showed a potential connection between the mechanism of ginsenoside Rg2 treatment for AD mice and lysosomes. The comprehensive results indicated that ginsenoside Rg2 may improve AD by downregulating LGMN, LAMP1, and PSAP through the regulation of the lysosomal pathway.</description><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>cognition</subject><subject>eosin</subject><subject>fluorescent antibody technique</subject><subject>food chemistry</subject><subject>ginsenosides</subject><subject>lysosomes</subject><subject>memory disorders</subject><subject>neurodegenerative diseases</subject><subject>neurons</subject><subject>pharmacology</subject><subject>prediction</subject><subject>proteomics</subject><subject>scopolamine</subject><subject>transmission electron microscopy</subject><subject>Western blotting</subject><issn>0021-8561</issn><issn>1520-5118</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkU1P4zAQhq0VSFsK9z36uAdSZhK7cY8IKFupiLLAOXKdSesqsUvsgPpn9reu-bhzGunVo5nR-zD2C2GCkOOFNmGy042ZCAOACn-wEcocMomojtgIEpMpOcWf7CSEHQAoWcKI_Zv7oc-ubUcuWO90y5d6TW0274n4w6BdtFFH-0p81ftIvrMmcN_wW-sCOR9sTfzvJueXHbXW9zpSzR-N3_tWd9ZRtnD1YFJ2R53vD3zR7bXt07HIreN31hCP294Pm22axJeH4IPv0hcrHbdv-nDKjhvdBjr7mmP2PL95uvqTLe9vF1eXy0yjUjFbCzOTs8aYQgDhWkhSupAo6qkqUchSSwFrxGkuVF1IA6IpKLUmgahphKJizH5_7t33_mWgEKvOBkNtqx35IVQFymIqcszF9yiUUM5yEJDQ8080yal2qejUb6gQqndj1UeYjFVfxor_nyOM9Q</recordid><startdate>20240703</startdate><enddate>20240703</enddate><creator>Yin, Pei</creator><creator>Wang, Heyu</creator><creator>Xue, Tingfang</creator><creator>Yu, Xiaoran</creator><creator>Meng, Xingjian</creator><creator>Mi, Qianwen</creator><creator>Song, Shixin</creator><creator>Xiong, Boyu</creator><creator>Bi, Yunfeng</creator><creator>Yu, Lei</creator><general>American Chemical Society</general><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0009-0006-4166-2424</orcidid><orcidid>https://orcid.org/0000-0002-6934-1319</orcidid></search><sort><creationdate>20240703</creationdate><title>Four-Dimensional Label-Free Quantitative Proteomics of Ginsenoside Rg2 Ameliorated Scopolamine-Induced Memory Impairment in Mice through the Lysosomal Pathway</title><author>Yin, Pei ; Wang, Heyu ; Xue, Tingfang ; Yu, Xiaoran ; Meng, Xingjian ; Mi, Qianwen ; Song, Shixin ; Xiong, Boyu ; Bi, Yunfeng ; Yu, Lei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a188t-b4c959fcc340e1b45e8a3514d6871457a540b116248d35c04f3e10250eeff48e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>cognition</topic><topic>eosin</topic><topic>fluorescent antibody technique</topic><topic>food chemistry</topic><topic>ginsenosides</topic><topic>lysosomes</topic><topic>memory disorders</topic><topic>neurodegenerative diseases</topic><topic>neurons</topic><topic>pharmacology</topic><topic>prediction</topic><topic>proteomics</topic><topic>scopolamine</topic><topic>transmission electron microscopy</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yin, Pei</creatorcontrib><creatorcontrib>Wang, Heyu</creatorcontrib><creatorcontrib>Xue, Tingfang</creatorcontrib><creatorcontrib>Yu, Xiaoran</creatorcontrib><creatorcontrib>Meng, Xingjian</creatorcontrib><creatorcontrib>Mi, Qianwen</creatorcontrib><creatorcontrib>Song, Shixin</creatorcontrib><creatorcontrib>Xiong, Boyu</creatorcontrib><creatorcontrib>Bi, Yunfeng</creatorcontrib><creatorcontrib>Yu, Lei</creatorcontrib><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Pei</au><au>Wang, Heyu</au><au>Xue, Tingfang</au><au>Yu, Xiaoran</au><au>Meng, Xingjian</au><au>Mi, Qianwen</au><au>Song, Shixin</au><au>Xiong, Boyu</au><au>Bi, Yunfeng</au><au>Yu, Lei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Four-Dimensional Label-Free Quantitative Proteomics of Ginsenoside Rg2 Ameliorated Scopolamine-Induced Memory Impairment in Mice through the Lysosomal Pathway</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2024-07-03</date><risdate>2024</risdate><volume>72</volume><issue>26</issue><spage>14640</spage><epage>14652</epage><pages>14640-14652</pages><issn>0021-8561</issn><issn>1520-5118</issn><eissn>1520-5118</eissn><abstract>Alzheimer’s disease (AD) is a neurodegenerative disease. Ginsenoside Rg2 has shown potential in treating AD, but the underlying protein regulatory mechanisms associated with ginsenoside Rg2 treatment for AD remain unclear. This study utilized scopolamine to induce memory impairment in mice, and proteomics methods were employed to investigate the potential molecular mechanism of ginsenoside Rg2 in treating AD model mice. The Morris water maze, hematoxylin and eosin staining, and Nissl staining results indicated that ginsenoside Rg2 enhanced cognitive ability and decreased neuronal damage in AD mice. Proteomics, western blot, and immunofluorescence results showed that ginsenoside Rg2 primarily improved AD mice by downregulating the expression of LGMN, LAMP1, and PSAP proteins through the regulation of the lysosomal pathway. Transmission electron microscopy and network pharmacology prediction results showed a potential connection between the mechanism of ginsenoside Rg2 treatment for AD mice and lysosomes. The comprehensive results indicated that ginsenoside Rg2 may improve AD by downregulating LGMN, LAMP1, and PSAP through the regulation of the lysosomal pathway.</abstract><pub>American Chemical Society</pub><doi>10.1021/acs.jafc.4c00181</doi><tpages>13</tpages><orcidid>https://orcid.org/0009-0006-4166-2424</orcidid><orcidid>https://orcid.org/0000-0002-6934-1319</orcidid></addata></record> |
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| subjects | Bioactive Constituents, Metabolites, and Functions cognition eosin fluorescent antibody technique food chemistry ginsenosides lysosomes memory disorders neurodegenerative diseases neurons pharmacology prediction proteomics scopolamine transmission electron microscopy Western blotting |
| title | Four-Dimensional Label-Free Quantitative Proteomics of Ginsenoside Rg2 Ameliorated Scopolamine-Induced Memory Impairment in Mice through the Lysosomal Pathway |
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