Transcriptomics Reveals the Mechanism of Purpureocillium lilacinum GZAC18-2JMP in Degrading Keratin Material
Microbial degradation of keratin is characterized by its inherent safety, remarkable efficiency, and the production of copious degradation products. All these attributes contribute to the effective management of waste materials at high value-added and in a sustainable manner. Microbial degradation o...
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| Veröffentlicht in: | Current microbiology 2024-08, Vol.81 (8), p.227, Article 227 |
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| creator | Han, Shumei Lu, Yingxia Peng, Lan Dong, Xuan Zhu, Liping Han, Yanfeng |
| description | Microbial degradation of keratin is characterized by its inherent safety, remarkable efficiency, and the production of copious degradation products. All these attributes contribute to the effective management of waste materials at high value-added and in a sustainable manner. Microbial degradation of keratin materials remains unclear, however, with variations observed in the degradation genes and pathways among different microorganisms. In this study, we sequenced the transcriptome of
Purpureocillium lilacinum
GZAC18-2JMP mycelia on control medium and the medium containing 1% feather powder, analyzed the differentially expressed genes, and revealed the degradation mechanism of chicken feathers by
P
.
lilacinum
GZAC18-2JMP. The results showed that the chicken feather degradation rate of
P
.
lilacinum
GZAC18-2JMP reached 64% after 216 h of incubation in the fermentation medium, reaching a peak value of 148.9 μg·mL
−1
at 192 h, and the keratinase enzyme activity reached a peak value of 211 U·mL
−1
at 168 h, which revealed that
P
.
lilacinum
GZAC18-2JMP had a better keratin degradation effect. A total of 1001 differentially expressed genes (DEGs) were identified from the transcriptome database, including 475 upregulated genes and 577 downregulated genes. Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis of the DEGs revealed that the metabolic pathways related to keratin degradation were mainly sulfur metabolism, ABC transporters, and amino acid metabolism. Therefore, the results of this study provide an opportunity to gain further insight into keratin degradation and promote the biotransformation of feather wastes. |
| doi_str_mv | 10.1007/s00284-024-03757-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3068757690</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3068631367</sourcerecordid><originalsourceid>FETCH-LOGICAL-c256t-64b89788b4d58ffc109aa94de36041fadd1fc7b7839d3f6c72b70f3a0b27df1e3</originalsourceid><addsrcrecordid>eNp9kU9P3DAQxa2qVdlSvgAHZKmXXlLGduI_R7RtaQurIgQXLpbj2IuRkyx2Umm_fV0WqMSBgzWW5zdvrPcQOiTwhQCI4wxAZV0BLYeJRlTbN2hBakYrUIq8RQtgNaskb8ge-pDzHQChCsh7tMekFEo2zQLFq2SGbFPYTGMfbMaX7o8zMePp1uGVs7dmCLnHo8cXc9rMyY02xBjmHscQjQ1DuZ3enCyJrOiv1QUOA_7q1sl0YVjjM5fMVF5WZnIpmPgRvfNF2x081n10_f3b1fJHdf779Ofy5LyytOFTxetWKiFlW3eN9N4SUMaounOMQ0286TrirWiFZKpjnltBWwGeGWip6DxxbB993ulu0ng_uzzpPmTrYjSDG-esGXBZ_OIKCvrpBXo3zmkov3ugOCOMi0LRHWXTmHNyXm9S6E3aagL6XxZ6l4UuWeiHLPS2DB09Ss9t77rnkSfzC8B2QC6tYe3S_92vyP4FxMiVQQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3068631367</pqid></control><display><type>article</type><title>Transcriptomics Reveals the Mechanism of Purpureocillium lilacinum GZAC18-2JMP in Degrading Keratin Material</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Han, Shumei ; Lu, Yingxia ; Peng, Lan ; Dong, Xuan ; Zhu, Liping ; Han, Yanfeng</creator><creatorcontrib>Han, Shumei ; Lu, Yingxia ; Peng, Lan ; Dong, Xuan ; Zhu, Liping ; Han, Yanfeng</creatorcontrib><description>Microbial degradation of keratin is characterized by its inherent safety, remarkable efficiency, and the production of copious degradation products. All these attributes contribute to the effective management of waste materials at high value-added and in a sustainable manner. Microbial degradation of keratin materials remains unclear, however, with variations observed in the degradation genes and pathways among different microorganisms. In this study, we sequenced the transcriptome of
Purpureocillium lilacinum
GZAC18-2JMP mycelia on control medium and the medium containing 1% feather powder, analyzed the differentially expressed genes, and revealed the degradation mechanism of chicken feathers by
P
.
lilacinum
GZAC18-2JMP. The results showed that the chicken feather degradation rate of
P
.
lilacinum
GZAC18-2JMP reached 64% after 216 h of incubation in the fermentation medium, reaching a peak value of 148.9 μg·mL
−1
at 192 h, and the keratinase enzyme activity reached a peak value of 211 U·mL
−1
at 168 h, which revealed that
P
.
lilacinum
GZAC18-2JMP had a better keratin degradation effect. A total of 1001 differentially expressed genes (DEGs) were identified from the transcriptome database, including 475 upregulated genes and 577 downregulated genes. Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis of the DEGs revealed that the metabolic pathways related to keratin degradation were mainly sulfur metabolism, ABC transporters, and amino acid metabolism. Therefore, the results of this study provide an opportunity to gain further insight into keratin degradation and promote the biotransformation of feather wastes.</description><identifier>ISSN: 0343-8651</identifier><identifier>ISSN: 1432-0991</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-024-03757-y</identifier><identifier>PMID: 38879855</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Amino acids ; Animals ; Biodegradation ; Biodegradation, Environmental ; Biomedical and Life Sciences ; Biotechnology ; Biotransformation ; Chickens ; Degradation ; Degradation products ; Enzymatic activity ; Enzyme activity ; Feathers ; Feathers - metabolism ; Fermentation ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Expression Profiling ; Genes ; Hypocreales - genetics ; Hypocreales - metabolism ; Keratin ; Keratinase ; Keratins - metabolism ; Life Sciences ; Metabolic pathways ; Metabolism ; Microbial degradation ; Microbiology ; Microorganisms ; Mycelium - genetics ; Mycelium - growth & development ; Mycelium - metabolism ; Peptide Hydrolases - genetics ; Peptide Hydrolases - metabolism ; Purpureocillium lilacinum ; Sulfur ; Transcriptome ; Transcriptomes ; Transcriptomics ; Waste management ; Waste materials</subject><ispartof>Current microbiology, 2024-08, Vol.81 (8), p.227, Article 227</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c256t-64b89788b4d58ffc109aa94de36041fadd1fc7b7839d3f6c72b70f3a0b27df1e3</cites><orcidid>0000-0002-8646-3975</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00284-024-03757-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00284-024-03757-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38879855$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Shumei</creatorcontrib><creatorcontrib>Lu, Yingxia</creatorcontrib><creatorcontrib>Peng, Lan</creatorcontrib><creatorcontrib>Dong, Xuan</creatorcontrib><creatorcontrib>Zhu, Liping</creatorcontrib><creatorcontrib>Han, Yanfeng</creatorcontrib><title>Transcriptomics Reveals the Mechanism of Purpureocillium lilacinum GZAC18-2JMP in Degrading Keratin Material</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>Microbial degradation of keratin is characterized by its inherent safety, remarkable efficiency, and the production of copious degradation products. All these attributes contribute to the effective management of waste materials at high value-added and in a sustainable manner. Microbial degradation of keratin materials remains unclear, however, with variations observed in the degradation genes and pathways among different microorganisms. In this study, we sequenced the transcriptome of
Purpureocillium lilacinum
GZAC18-2JMP mycelia on control medium and the medium containing 1% feather powder, analyzed the differentially expressed genes, and revealed the degradation mechanism of chicken feathers by
P
.
lilacinum
GZAC18-2JMP. The results showed that the chicken feather degradation rate of
P
.
lilacinum
GZAC18-2JMP reached 64% after 216 h of incubation in the fermentation medium, reaching a peak value of 148.9 μg·mL
−1
at 192 h, and the keratinase enzyme activity reached a peak value of 211 U·mL
−1
at 168 h, which revealed that
P
.
lilacinum
GZAC18-2JMP had a better keratin degradation effect. A total of 1001 differentially expressed genes (DEGs) were identified from the transcriptome database, including 475 upregulated genes and 577 downregulated genes. Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis of the DEGs revealed that the metabolic pathways related to keratin degradation were mainly sulfur metabolism, ABC transporters, and amino acid metabolism. Therefore, the results of this study provide an opportunity to gain further insight into keratin degradation and promote the biotransformation of feather wastes.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Biodegradation</subject><subject>Biodegradation, Environmental</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Biotransformation</subject><subject>Chickens</subject><subject>Degradation</subject><subject>Degradation products</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Feathers</subject><subject>Feathers - metabolism</subject><subject>Fermentation</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Genes</subject><subject>Hypocreales - genetics</subject><subject>Hypocreales - metabolism</subject><subject>Keratin</subject><subject>Keratinase</subject><subject>Keratins - metabolism</subject><subject>Life Sciences</subject><subject>Metabolic pathways</subject><subject>Metabolism</subject><subject>Microbial degradation</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Mycelium - genetics</subject><subject>Mycelium - growth & development</subject><subject>Mycelium - metabolism</subject><subject>Peptide Hydrolases - genetics</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Purpureocillium lilacinum</subject><subject>Sulfur</subject><subject>Transcriptome</subject><subject>Transcriptomes</subject><subject>Transcriptomics</subject><subject>Waste management</subject><subject>Waste materials</subject><issn>0343-8651</issn><issn>1432-0991</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9P3DAQxa2qVdlSvgAHZKmXXlLGduI_R7RtaQurIgQXLpbj2IuRkyx2Umm_fV0WqMSBgzWW5zdvrPcQOiTwhQCI4wxAZV0BLYeJRlTbN2hBakYrUIq8RQtgNaskb8ge-pDzHQChCsh7tMekFEo2zQLFq2SGbFPYTGMfbMaX7o8zMePp1uGVs7dmCLnHo8cXc9rMyY02xBjmHscQjQ1DuZ3enCyJrOiv1QUOA_7q1sl0YVjjM5fMVF5WZnIpmPgRvfNF2x081n10_f3b1fJHdf779Ofy5LyytOFTxetWKiFlW3eN9N4SUMaounOMQ0286TrirWiFZKpjnltBWwGeGWip6DxxbB993ulu0ng_uzzpPmTrYjSDG-esGXBZ_OIKCvrpBXo3zmkov3ugOCOMi0LRHWXTmHNyXm9S6E3aagL6XxZ6l4UuWeiHLPS2DB09Ss9t77rnkSfzC8B2QC6tYe3S_92vyP4FxMiVQQ</recordid><startdate>20240801</startdate><enddate>20240801</enddate><creator>Han, Shumei</creator><creator>Lu, Yingxia</creator><creator>Peng, Lan</creator><creator>Dong, Xuan</creator><creator>Zhu, Liping</creator><creator>Han, Yanfeng</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8646-3975</orcidid></search><sort><creationdate>20240801</creationdate><title>Transcriptomics Reveals the Mechanism of Purpureocillium lilacinum GZAC18-2JMP in Degrading Keratin Material</title><author>Han, Shumei ; Lu, Yingxia ; Peng, Lan ; Dong, Xuan ; Zhu, Liping ; Han, Yanfeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-64b89788b4d58ffc109aa94de36041fadd1fc7b7839d3f6c72b70f3a0b27df1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Amino acids</topic><topic>Animals</topic><topic>Biodegradation</topic><topic>Biodegradation, Environmental</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Biotransformation</topic><topic>Chickens</topic><topic>Degradation</topic><topic>Degradation products</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Feathers</topic><topic>Feathers - metabolism</topic><topic>Fermentation</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Expression Profiling</topic><topic>Genes</topic><topic>Hypocreales - genetics</topic><topic>Hypocreales - metabolism</topic><topic>Keratin</topic><topic>Keratinase</topic><topic>Keratins - metabolism</topic><topic>Life Sciences</topic><topic>Metabolic pathways</topic><topic>Metabolism</topic><topic>Microbial degradation</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Mycelium - genetics</topic><topic>Mycelium - growth & development</topic><topic>Mycelium - metabolism</topic><topic>Peptide Hydrolases - genetics</topic><topic>Peptide Hydrolases - metabolism</topic><topic>Purpureocillium lilacinum</topic><topic>Sulfur</topic><topic>Transcriptome</topic><topic>Transcriptomes</topic><topic>Transcriptomics</topic><topic>Waste management</topic><topic>Waste materials</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Shumei</creatorcontrib><creatorcontrib>Lu, Yingxia</creatorcontrib><creatorcontrib>Peng, Lan</creatorcontrib><creatorcontrib>Dong, Xuan</creatorcontrib><creatorcontrib>Zhu, Liping</creatorcontrib><creatorcontrib>Han, Yanfeng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Shumei</au><au>Lu, Yingxia</au><au>Peng, Lan</au><au>Dong, Xuan</au><au>Zhu, Liping</au><au>Han, Yanfeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptomics Reveals the Mechanism of Purpureocillium lilacinum GZAC18-2JMP in Degrading Keratin Material</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2024-08-01</date><risdate>2024</risdate><volume>81</volume><issue>8</issue><spage>227</spage><pages>227-</pages><artnum>227</artnum><issn>0343-8651</issn><issn>1432-0991</issn><eissn>1432-0991</eissn><abstract>Microbial degradation of keratin is characterized by its inherent safety, remarkable efficiency, and the production of copious degradation products. All these attributes contribute to the effective management of waste materials at high value-added and in a sustainable manner. Microbial degradation of keratin materials remains unclear, however, with variations observed in the degradation genes and pathways among different microorganisms. In this study, we sequenced the transcriptome of
Purpureocillium lilacinum
GZAC18-2JMP mycelia on control medium and the medium containing 1% feather powder, analyzed the differentially expressed genes, and revealed the degradation mechanism of chicken feathers by
P
.
lilacinum
GZAC18-2JMP. The results showed that the chicken feather degradation rate of
P
.
lilacinum
GZAC18-2JMP reached 64% after 216 h of incubation in the fermentation medium, reaching a peak value of 148.9 μg·mL
−1
at 192 h, and the keratinase enzyme activity reached a peak value of 211 U·mL
−1
at 168 h, which revealed that
P
.
lilacinum
GZAC18-2JMP had a better keratin degradation effect. A total of 1001 differentially expressed genes (DEGs) were identified from the transcriptome database, including 475 upregulated genes and 577 downregulated genes. Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis of the DEGs revealed that the metabolic pathways related to keratin degradation were mainly sulfur metabolism, ABC transporters, and amino acid metabolism. Therefore, the results of this study provide an opportunity to gain further insight into keratin degradation and promote the biotransformation of feather wastes.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>38879855</pmid><doi>10.1007/s00284-024-03757-y</doi><orcidid>https://orcid.org/0000-0002-8646-3975</orcidid></addata></record> |
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| subjects | Amino acids Animals Biodegradation Biodegradation, Environmental Biomedical and Life Sciences Biotechnology Biotransformation Chickens Degradation Degradation products Enzymatic activity Enzyme activity Feathers Feathers - metabolism Fermentation Fungal Proteins - genetics Fungal Proteins - metabolism Gene Expression Profiling Genes Hypocreales - genetics Hypocreales - metabolism Keratin Keratinase Keratins - metabolism Life Sciences Metabolic pathways Metabolism Microbial degradation Microbiology Microorganisms Mycelium - genetics Mycelium - growth & development Mycelium - metabolism Peptide Hydrolases - genetics Peptide Hydrolases - metabolism Purpureocillium lilacinum Sulfur Transcriptome Transcriptomes Transcriptomics Waste management Waste materials |
| title | Transcriptomics Reveals the Mechanism of Purpureocillium lilacinum GZAC18-2JMP in Degrading Keratin Material |
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