Postmodification with Polycations Enhances Key Properties of Alginate-Based Multicomponent Microcapsules
Postmodification of alginate-based microspheres with polyelectrolytes (PEs) is commonly used in the cell encapsulation field to control microsphere stability and permeability. However, little is known about how different applied PEs shape the microsphere morphology and properties, particularly in vi...
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Veröffentlicht in: | Biomacromolecules 2024-07, Vol.25 (7), p.4118-4138 |
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creator | Dorchei, Faeze Heydari, Abolfazl Kroneková, Zuzana Kronek, Juraj Pelach, Michal Cseriová, Zuzana Chorvát, Dušan Zúñiga-Navarrete, Fernando Rios, Peter D. McGarrigle, James Ghani, Sofia Isa, Douglas Joshi, Ira Vasuthas, Kalaiyarasi Rokstad, Anne Mari A. Oberholzer, José Raus, Vladimír Lacík, Igor |
description | Postmodification of alginate-based microspheres with polyelectrolytes (PEs) is commonly used in the cell encapsulation field to control microsphere stability and permeability. However, little is known about how different applied PEs shape the microsphere morphology and properties, particularly in vivo. Here, we addressed this question using model multicomponent alginate-based microcapsules postmodified with PEs of different charge and structure. We found that the postmodification can enhance or impair the mechanical resistance and biocompatibility of microcapsules implanted into a mouse model, with polycations surprisingly providing the best results. Confocal Raman microscopy and confocal laser scanning microscopy (CLSM) analyses revealed stable interpolyelectrolyte complex layers within the parent microcapsule, hindering the access of higher molar weight PEs into the microcapsule core. All microcapsules showed negative surface zeta potential, indicating that the postmodification PEs get hidden within the microcapsule membrane, which agrees with CLSM data. Human whole blood assay revealed complex behavior of microcapsules regarding their inflammatory and coagulation potential. Importantly, most of the postmodification PEs, including polycations, were found to be benign toward the encapsulated model cells. |
doi_str_mv | 10.1021/acs.biomac.4c00222 |
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However, little is known about how different applied PEs shape the microsphere morphology and properties, particularly in vivo. Here, we addressed this question using model multicomponent alginate-based microcapsules postmodified with PEs of different charge and structure. We found that the postmodification can enhance or impair the mechanical resistance and biocompatibility of microcapsules implanted into a mouse model, with polycations surprisingly providing the best results. Confocal Raman microscopy and confocal laser scanning microscopy (CLSM) analyses revealed stable interpolyelectrolyte complex layers within the parent microcapsule, hindering the access of higher molar weight PEs into the microcapsule core. All microcapsules showed negative surface zeta potential, indicating that the postmodification PEs get hidden within the microcapsule membrane, which agrees with CLSM data. Human whole blood assay revealed complex behavior of microcapsules regarding their inflammatory and coagulation potential. 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Human whole blood assay revealed complex behavior of microcapsules regarding their inflammatory and coagulation potential. 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title | Postmodification with Polycations Enhances Key Properties of Alginate-Based Multicomponent Microcapsules |
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