Comprehensive transcriptome profiling between balding and non-balding scalp of female pattern hair loss in Asian
While many gene expression studies have focused on male pattern baldness (MPB), few studies have investigated the genetic differences between bald and non-bald hair follicles in female pattern hair loss (FPHL). This study aimed to identify molecular biomarkers associated with FPHL through genetic an...
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| Veröffentlicht in: | Archives of dermatological research 2024-06, Vol.316 (7), p.360, Article 360 |
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| description | While many gene expression studies have focused on male pattern baldness (MPB), few studies have investigated the genetic differences between bald and non-bald hair follicles in female pattern hair loss (FPHL). This study aimed to identify molecular biomarkers associated with FPHL through genetic analysis of paired bald and non-bald hair follicles from 18 FPHL patients, using next-generation sequencing (NGS) techniques. RNA transcriptome analysis was performed to identify differentially expressed genes (DEGs) between bald and non-bald hair follicles in FPHL. The DEGs were validated using real-time PCR, and protein expression was confirmed through immunohistochemistry and western blot analysis. Our findings suggest that HOXB13, SFRP2, PTGDS, CXCR3, SFRP4, SOD3, and DCN are significantly upregulated in bald hair follicles compared to non-bald hair follicles in FPHL. SFRP2 and PTGDS were found to be consistently highly expressed in bald hair follicles in all 18 samples. Additionally, elevated protein levels of SFRP2 and PTGDS were confirmed through western blot and immunohistochemical analysis. Our study identified SFRP2 and PTGDS as potential biomarkers for FPHL and suggests that they may play a role in inducing hair loss in this condition. These findings provide a foundation for further research on the pathogenesis of FPHL and potential therapeutic targets. |
| doi_str_mv | 10.1007/s00403-024-03114-w |
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This study aimed to identify molecular biomarkers associated with FPHL through genetic analysis of paired bald and non-bald hair follicles from 18 FPHL patients, using next-generation sequencing (NGS) techniques. RNA transcriptome analysis was performed to identify differentially expressed genes (DEGs) between bald and non-bald hair follicles in FPHL. The DEGs were validated using real-time PCR, and protein expression was confirmed through immunohistochemistry and western blot analysis. Our findings suggest that HOXB13, SFRP2, PTGDS, CXCR3, SFRP4, SOD3, and DCN are significantly upregulated in bald hair follicles compared to non-bald hair follicles in FPHL. SFRP2 and PTGDS were found to be consistently highly expressed in bald hair follicles in all 18 samples. Additionally, elevated protein levels of SFRP2 and PTGDS were confirmed through western blot and immunohistochemical analysis. Our study identified SFRP2 and PTGDS as potential biomarkers for FPHL and suggests that they may play a role in inducing hair loss in this condition. These findings provide a foundation for further research on the pathogenesis of FPHL and potential therapeutic targets.</description><identifier>ISSN: 1432-069X</identifier><identifier>ISSN: 0340-3696</identifier><identifier>EISSN: 1432-069X</identifier><identifier>DOI: 10.1007/s00403-024-03114-w</identifier><identifier>PMID: 38850442</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adult ; Alopecia - genetics ; Alopecia - pathology ; Asian People - genetics ; Biomarkers ; CXCR3 protein ; Dermatology ; Female ; Follicles ; Gene expression ; Gene Expression Profiling ; Genetic analysis ; Hair ; Hair Follicle - metabolism ; Hair Follicle - pathology ; Hair loss ; High-Throughput Nucleotide Sequencing ; Humans ; Immunohistochemistry ; Medicine ; Medicine & Public Health ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Middle Aged ; Next-generation sequencing ; Proto-Oncogene Proteins ; Scalp - pathology ; Study Protocol ; Therapeutic targets ; Transcriptome ; Transcriptomes ; Young Adult</subject><ispartof>Archives of dermatological research, 2024-06, Vol.316 (7), p.360, Article 360</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c256t-9ff51b6f77e77c46b8ba27867890ebb0b61d7397a9ef25a649e5d242bd4ea4a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00403-024-03114-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00403-024-03114-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38850442$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Soon Re</creatorcontrib><creatorcontrib>Kim, Yun Ji</creatorcontrib><creatorcontrib>Kim, Ju-Hee</creatorcontrib><creatorcontrib>Kim, Su Na</creatorcontrib><creatorcontrib>Park, Won Serk</creatorcontrib><creatorcontrib>Kim, Se Hwan</creatorcontrib><creatorcontrib>Chung, Juhyun</creatorcontrib><creatorcontrib>Choi, Mi soo</creatorcontrib><creatorcontrib>Kim, MyungHwa</creatorcontrib><creatorcontrib>Park, Byung Cheol</creatorcontrib><title>Comprehensive transcriptome profiling between balding and non-balding scalp of female pattern hair loss in Asian</title><title>Archives of dermatological research</title><addtitle>Arch Dermatol Res</addtitle><addtitle>Arch Dermatol Res</addtitle><description>While many gene expression studies have focused on male pattern baldness (MPB), few studies have investigated the genetic differences between bald and non-bald hair follicles in female pattern hair loss (FPHL). This study aimed to identify molecular biomarkers associated with FPHL through genetic analysis of paired bald and non-bald hair follicles from 18 FPHL patients, using next-generation sequencing (NGS) techniques. RNA transcriptome analysis was performed to identify differentially expressed genes (DEGs) between bald and non-bald hair follicles in FPHL. The DEGs were validated using real-time PCR, and protein expression was confirmed through immunohistochemistry and western blot analysis. Our findings suggest that HOXB13, SFRP2, PTGDS, CXCR3, SFRP4, SOD3, and DCN are significantly upregulated in bald hair follicles compared to non-bald hair follicles in FPHL. SFRP2 and PTGDS were found to be consistently highly expressed in bald hair follicles in all 18 samples. Additionally, elevated protein levels of SFRP2 and PTGDS were confirmed through western blot and immunohistochemical analysis. Our study identified SFRP2 and PTGDS as potential biomarkers for FPHL and suggests that they may play a role in inducing hair loss in this condition. These findings provide a foundation for further research on the pathogenesis of FPHL and potential therapeutic targets.</description><subject>Adult</subject><subject>Alopecia - genetics</subject><subject>Alopecia - pathology</subject><subject>Asian People - genetics</subject><subject>Biomarkers</subject><subject>CXCR3 protein</subject><subject>Dermatology</subject><subject>Female</subject><subject>Follicles</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Genetic analysis</subject><subject>Hair</subject><subject>Hair Follicle - metabolism</subject><subject>Hair Follicle - pathology</subject><subject>Hair loss</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Middle Aged</subject><subject>Next-generation sequencing</subject><subject>Proto-Oncogene Proteins</subject><subject>Scalp - pathology</subject><subject>Study Protocol</subject><subject>Therapeutic targets</subject><subject>Transcriptome</subject><subject>Transcriptomes</subject><subject>Young Adult</subject><issn>1432-069X</issn><issn>0340-3696</issn><issn>1432-069X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtLxTAQhYMovv-ACwm4cVPNq0m7lIsvENwouAtJO9FIm9ak14v_3lyvL1y4Sob5zklmDkIHlJxQQtRpIkQQXhAmCsIpFcViDW1TwVlBZP2w_uu-hXZSeiZZpCq6ibZ4VZVECLaNxtnQjxGeICT_CniKJqQm-nEaesBjHJzvfHjEFqYFQMDWdO2yNqHFYQjFV50a0414cNhBb7qsNNMEMeAn4yPuhpSwD_gseRP20IYzXYL9z3MX3V-c382uipvby-vZ2U3RsFJORe1cSa10SoFSjZC2soapSqqqJmAtsZK2itfK1OBYaaSooWyZYLYVYIRRfBcdr3zzEC9zSJPufWqg60yAYZ40J7KsVcXoEj36gz4P8xjy7zSnebtc1oxmiq2oJuZ5Ijg9Rt-b-KYp0cs89CoPnRX6Iw-9yKLDT-u57aH9lnwFkAG-AlJuhUeIP2__Y_sOWnSXjg</recordid><startdate>20240608</startdate><enddate>20240608</enddate><creator>Kim, Soon Re</creator><creator>Kim, Yun Ji</creator><creator>Kim, Ju-Hee</creator><creator>Kim, Su Na</creator><creator>Park, Won Serk</creator><creator>Kim, Se Hwan</creator><creator>Chung, Juhyun</creator><creator>Choi, Mi soo</creator><creator>Kim, MyungHwa</creator><creator>Park, Byung Cheol</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20240608</creationdate><title>Comprehensive transcriptome profiling between balding and non-balding scalp of female pattern hair loss in Asian</title><author>Kim, Soon Re ; Kim, Yun Ji ; Kim, Ju-Hee ; Kim, Su Na ; Park, Won Serk ; Kim, Se Hwan ; Chung, Juhyun ; Choi, Mi soo ; Kim, MyungHwa ; Park, Byung Cheol</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-9ff51b6f77e77c46b8ba27867890ebb0b61d7397a9ef25a649e5d242bd4ea4a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adult</topic><topic>Alopecia - genetics</topic><topic>Alopecia - pathology</topic><topic>Asian People - genetics</topic><topic>Biomarkers</topic><topic>CXCR3 protein</topic><topic>Dermatology</topic><topic>Female</topic><topic>Follicles</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Genetic analysis</topic><topic>Hair</topic><topic>Hair Follicle - metabolism</topic><topic>Hair Follicle - pathology</topic><topic>Hair loss</topic><topic>High-Throughput Nucleotide Sequencing</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Middle Aged</topic><topic>Next-generation sequencing</topic><topic>Proto-Oncogene Proteins</topic><topic>Scalp - pathology</topic><topic>Study Protocol</topic><topic>Therapeutic targets</topic><topic>Transcriptome</topic><topic>Transcriptomes</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Soon Re</creatorcontrib><creatorcontrib>Kim, Yun Ji</creatorcontrib><creatorcontrib>Kim, Ju-Hee</creatorcontrib><creatorcontrib>Kim, Su Na</creatorcontrib><creatorcontrib>Park, Won Serk</creatorcontrib><creatorcontrib>Kim, Se Hwan</creatorcontrib><creatorcontrib>Chung, Juhyun</creatorcontrib><creatorcontrib>Choi, Mi soo</creatorcontrib><creatorcontrib>Kim, MyungHwa</creatorcontrib><creatorcontrib>Park, Byung Cheol</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of dermatological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Soon Re</au><au>Kim, Yun Ji</au><au>Kim, Ju-Hee</au><au>Kim, Su Na</au><au>Park, Won Serk</au><au>Kim, Se Hwan</au><au>Chung, Juhyun</au><au>Choi, Mi soo</au><au>Kim, MyungHwa</au><au>Park, Byung Cheol</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comprehensive transcriptome profiling between balding and non-balding scalp of female pattern hair loss in Asian</atitle><jtitle>Archives of dermatological research</jtitle><stitle>Arch Dermatol Res</stitle><addtitle>Arch Dermatol Res</addtitle><date>2024-06-08</date><risdate>2024</risdate><volume>316</volume><issue>7</issue><spage>360</spage><pages>360-</pages><artnum>360</artnum><issn>1432-069X</issn><issn>0340-3696</issn><eissn>1432-069X</eissn><abstract>While many gene expression studies have focused on male pattern baldness (MPB), few studies have investigated the genetic differences between bald and non-bald hair follicles in female pattern hair loss (FPHL). This study aimed to identify molecular biomarkers associated with FPHL through genetic analysis of paired bald and non-bald hair follicles from 18 FPHL patients, using next-generation sequencing (NGS) techniques. RNA transcriptome analysis was performed to identify differentially expressed genes (DEGs) between bald and non-bald hair follicles in FPHL. The DEGs were validated using real-time PCR, and protein expression was confirmed through immunohistochemistry and western blot analysis. Our findings suggest that HOXB13, SFRP2, PTGDS, CXCR3, SFRP4, SOD3, and DCN are significantly upregulated in bald hair follicles compared to non-bald hair follicles in FPHL. SFRP2 and PTGDS were found to be consistently highly expressed in bald hair follicles in all 18 samples. Additionally, elevated protein levels of SFRP2 and PTGDS were confirmed through western blot and immunohistochemical analysis. Our study identified SFRP2 and PTGDS as potential biomarkers for FPHL and suggests that they may play a role in inducing hair loss in this condition. These findings provide a foundation for further research on the pathogenesis of FPHL and potential therapeutic targets.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38850442</pmid><doi>10.1007/s00403-024-03114-w</doi></addata></record> |
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| subjects | Adult Alopecia - genetics Alopecia - pathology Asian People - genetics Biomarkers CXCR3 protein Dermatology Female Follicles Gene expression Gene Expression Profiling Genetic analysis Hair Hair Follicle - metabolism Hair Follicle - pathology Hair loss High-Throughput Nucleotide Sequencing Humans Immunohistochemistry Medicine Medicine & Public Health Membrane Proteins - genetics Membrane Proteins - metabolism Middle Aged Next-generation sequencing Proto-Oncogene Proteins Scalp - pathology Study Protocol Therapeutic targets Transcriptome Transcriptomes Young Adult |
| title | Comprehensive transcriptome profiling between balding and non-balding scalp of female pattern hair loss in Asian |
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