Heparin-Functionalized Bioactive Glass to Harvest Endogenous Growth Factors for Pulp Regeneration
Pulp and periapical diseases can lead to the cessation of tooth development, resulting in compromised tooth structure and functions. Despite numerous efforts to induce pulp regeneration, effective strategies are still lacking. Growth factors (GFs) hold considerable promise in pulp regeneration due t...
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description | Pulp and periapical diseases can lead to the cessation of tooth development, resulting in compromised tooth structure and functions. Despite numerous efforts to induce pulp regeneration, effective strategies are still lacking. Growth factors (GFs) hold considerable promise in pulp regeneration due to their diverse cellular regulatory properties. However, the limited half-lives and susceptibility to degradation of exogenous GFs necessitate the administration of supra-physiological doses, leading to undesirable side effects. In this research, a heparin-functionalized bioactive glass (CaO–P2O5–SiO2–Heparin, abbreviated as PSC-Heparin) with strong bioactivity and a stable neutral pH is developed as a promising candidate to addressing challenges in pulp regeneration. Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis reveal the successful synthesis of PSC-Heparin. Scanning electron microscopy and X-ray diffraction show the hydroxyapatite formation can be observed on the surface of PSC-Heparin after soaking in simulated body fluid for 12 h. PSC-Heparin is capable of harvesting various endogenous GFs and sustainably releasing them over an extended duration by the enzyme-linked immunosorbent assay. Cytological experiments show that developed PSC-Heparin can facilitate the adhesion, migration, proliferation, and odontogenic differentiation of stem cells from apical papillae. Notably, the histological analysis of subcutaneous implantation in nude mice demonstrates PSC-Heparin is capable of promoting the odontoblast-like layers and pulp-dentin complex formation without the addition of exogenous GFs, which is vital for clinical applications. This work highlights an effective strategy of harvesting endogenous GFs and avoiding the involvement of exogenous GFs to achieve pulp-dentin complex regeneration, which may open a new horizon for regenerative endodontic therapy. |
doi_str_mv | 10.1021/acsami.4c03118 |
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Despite numerous efforts to induce pulp regeneration, effective strategies are still lacking. Growth factors (GFs) hold considerable promise in pulp regeneration due to their diverse cellular regulatory properties. However, the limited half-lives and susceptibility to degradation of exogenous GFs necessitate the administration of supra-physiological doses, leading to undesirable side effects. In this research, a heparin-functionalized bioactive glass (CaO–P2O5–SiO2–Heparin, abbreviated as PSC-Heparin) with strong bioactivity and a stable neutral pH is developed as a promising candidate to addressing challenges in pulp regeneration. Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis reveal the successful synthesis of PSC-Heparin. Scanning electron microscopy and X-ray diffraction show the hydroxyapatite formation can be observed on the surface of PSC-Heparin after soaking in simulated body fluid for 12 h. PSC-Heparin is capable of harvesting various endogenous GFs and sustainably releasing them over an extended duration by the enzyme-linked immunosorbent assay. Cytological experiments show that developed PSC-Heparin can facilitate the adhesion, migration, proliferation, and odontogenic differentiation of stem cells from apical papillae. Notably, the histological analysis of subcutaneous implantation in nude mice demonstrates PSC-Heparin is capable of promoting the odontoblast-like layers and pulp-dentin complex formation without the addition of exogenous GFs, which is vital for clinical applications. This work highlights an effective strategy of harvesting endogenous GFs and avoiding the involvement of exogenous GFs to achieve pulp-dentin complex regeneration, which may open a new horizon for regenerative endodontic therapy.</description><identifier>ISSN: 1944-8244</identifier><identifier>ISSN: 1944-8252</identifier><identifier>EISSN: 1944-8252</identifier><identifier>DOI: 10.1021/acsami.4c03118</identifier><identifier>PMID: 38833722</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Biological and Medical Applications of Materials and Interfaces ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Dental Pulp - cytology ; Dental Pulp - drug effects ; Dental Pulp - metabolism ; Glass - chemistry ; Heparin - chemistry ; Heparin - pharmacology ; Humans ; Intercellular Signaling Peptides and Proteins - chemistry ; Intercellular Signaling Peptides and Proteins - pharmacology ; Mice ; Mice, Nude ; Regeneration - drug effects ; Stem Cells - cytology ; Stem Cells - drug effects ; Stem Cells - metabolism</subject><ispartof>ACS applied materials & interfaces, 2024-06, Vol.16 (24), p.30715-30727</ispartof><rights>2024 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a215t-f92d5e86f0dd36ba326354afd633db34c1e420fb010461bd7a9ba52afd0b4cf73</cites><orcidid>0000-0001-6320-0913 ; 0000-0002-2287-6084 ; 0000-0002-2749-7788</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acsami.4c03118$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acsami.4c03118$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38833722$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Jilin</creatorcontrib><creatorcontrib>Li, Jingyi</creatorcontrib><creatorcontrib>Mao, Sicong</creatorcontrib><creatorcontrib>Li, Baokui</creatorcontrib><creatorcontrib>Zhu, Lin</creatorcontrib><creatorcontrib>Jia, Peipei</creatorcontrib><creatorcontrib>Huang, Guibin</creatorcontrib><creatorcontrib>Yang, Xule</creatorcontrib><creatorcontrib>Xu, Liju</creatorcontrib><creatorcontrib>Qiu, Dong</creatorcontrib><creatorcontrib>Wang, Sainan</creatorcontrib><creatorcontrib>Dong, Yanmei</creatorcontrib><title>Heparin-Functionalized Bioactive Glass to Harvest Endogenous Growth Factors for Pulp Regeneration</title><title>ACS applied materials & interfaces</title><addtitle>ACS Appl. Mater. Interfaces</addtitle><description>Pulp and periapical diseases can lead to the cessation of tooth development, resulting in compromised tooth structure and functions. Despite numerous efforts to induce pulp regeneration, effective strategies are still lacking. Growth factors (GFs) hold considerable promise in pulp regeneration due to their diverse cellular regulatory properties. However, the limited half-lives and susceptibility to degradation of exogenous GFs necessitate the administration of supra-physiological doses, leading to undesirable side effects. In this research, a heparin-functionalized bioactive glass (CaO–P2O5–SiO2–Heparin, abbreviated as PSC-Heparin) with strong bioactivity and a stable neutral pH is developed as a promising candidate to addressing challenges in pulp regeneration. Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis reveal the successful synthesis of PSC-Heparin. Scanning electron microscopy and X-ray diffraction show the hydroxyapatite formation can be observed on the surface of PSC-Heparin after soaking in simulated body fluid for 12 h. PSC-Heparin is capable of harvesting various endogenous GFs and sustainably releasing them over an extended duration by the enzyme-linked immunosorbent assay. Cytological experiments show that developed PSC-Heparin can facilitate the adhesion, migration, proliferation, and odontogenic differentiation of stem cells from apical papillae. Notably, the histological analysis of subcutaneous implantation in nude mice demonstrates PSC-Heparin is capable of promoting the odontoblast-like layers and pulp-dentin complex formation without the addition of exogenous GFs, which is vital for clinical applications. This work highlights an effective strategy of harvesting endogenous GFs and avoiding the involvement of exogenous GFs to achieve pulp-dentin complex regeneration, which may open a new horizon for regenerative endodontic therapy.</description><subject>Animals</subject><subject>Biological and Medical Applications of Materials and Interfaces</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - drug effects</subject><subject>Dental Pulp - metabolism</subject><subject>Glass - chemistry</subject><subject>Heparin - chemistry</subject><subject>Heparin - pharmacology</subject><subject>Humans</subject><subject>Intercellular Signaling Peptides and Proteins - chemistry</subject><subject>Intercellular Signaling Peptides and Proteins - pharmacology</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Regeneration - drug effects</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - metabolism</subject><issn>1944-8244</issn><issn>1944-8252</issn><issn>1944-8252</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1Lw0AQhhdRbK1ePcoeRUjdr2yTo5Z-CAVF9LxMshtNSbJxN6nor3dLqjdPMwPP-zI8CF1SMqWE0VvIPdTlVOSEU5ocoTFNhYgSFrPjv12IETrzfkuI5IzEp2jEk4TzGWNjBGvTgiubaNk3eVfaBqry22h8X1oI987gVQXe487iNbid8R1eNNq-mcb2Hq-c_eze8TKg1nlcWIef-qrFzyYAxsG-8BydFFB5c3GYE_S6XLzM19HmcfUwv9tEwGjcRUXKdGwSWRCtucyAM8ljAYWWnOuMi5wawUiREUqEpJmeQZpBzAJAMpEXMz5B10Nv6-xHHx5VdelzU1XQmPCr4kSKlBFJSECnA5o7670zhWpdWYP7UpSovVY1aFUHrSFwdejus9roP_zXYwBuBiAE1db2Lnj0_7X9AEnMg7s</recordid><startdate>20240619</startdate><enddate>20240619</enddate><creator>Wu, Jilin</creator><creator>Li, Jingyi</creator><creator>Mao, Sicong</creator><creator>Li, Baokui</creator><creator>Zhu, Lin</creator><creator>Jia, Peipei</creator><creator>Huang, Guibin</creator><creator>Yang, Xule</creator><creator>Xu, Liju</creator><creator>Qiu, Dong</creator><creator>Wang, Sainan</creator><creator>Dong, Yanmei</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6320-0913</orcidid><orcidid>https://orcid.org/0000-0002-2287-6084</orcidid><orcidid>https://orcid.org/0000-0002-2749-7788</orcidid></search><sort><creationdate>20240619</creationdate><title>Heparin-Functionalized Bioactive Glass to Harvest Endogenous Growth Factors for Pulp Regeneration</title><author>Wu, Jilin ; Li, Jingyi ; Mao, Sicong ; Li, Baokui ; Zhu, Lin ; Jia, Peipei ; Huang, Guibin ; Yang, Xule ; Xu, Liju ; Qiu, Dong ; Wang, Sainan ; Dong, Yanmei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a215t-f92d5e86f0dd36ba326354afd633db34c1e420fb010461bd7a9ba52afd0b4cf73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Biological and Medical Applications of Materials and Interfaces</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Dental Pulp - cytology</topic><topic>Dental Pulp - drug effects</topic><topic>Dental Pulp - metabolism</topic><topic>Glass - chemistry</topic><topic>Heparin - chemistry</topic><topic>Heparin - pharmacology</topic><topic>Humans</topic><topic>Intercellular Signaling Peptides and Proteins - chemistry</topic><topic>Intercellular Signaling Peptides and Proteins - pharmacology</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Regeneration - drug effects</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Jilin</creatorcontrib><creatorcontrib>Li, Jingyi</creatorcontrib><creatorcontrib>Mao, Sicong</creatorcontrib><creatorcontrib>Li, Baokui</creatorcontrib><creatorcontrib>Zhu, Lin</creatorcontrib><creatorcontrib>Jia, Peipei</creatorcontrib><creatorcontrib>Huang, Guibin</creatorcontrib><creatorcontrib>Yang, Xule</creatorcontrib><creatorcontrib>Xu, Liju</creatorcontrib><creatorcontrib>Qiu, Dong</creatorcontrib><creatorcontrib>Wang, Sainan</creatorcontrib><creatorcontrib>Dong, Yanmei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS applied materials & interfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Jilin</au><au>Li, Jingyi</au><au>Mao, Sicong</au><au>Li, Baokui</au><au>Zhu, Lin</au><au>Jia, Peipei</au><au>Huang, Guibin</au><au>Yang, Xule</au><au>Xu, Liju</au><au>Qiu, Dong</au><au>Wang, Sainan</au><au>Dong, Yanmei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heparin-Functionalized Bioactive Glass to Harvest Endogenous Growth Factors for Pulp Regeneration</atitle><jtitle>ACS applied materials & interfaces</jtitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><date>2024-06-19</date><risdate>2024</risdate><volume>16</volume><issue>24</issue><spage>30715</spage><epage>30727</epage><pages>30715-30727</pages><issn>1944-8244</issn><issn>1944-8252</issn><eissn>1944-8252</eissn><abstract>Pulp and periapical diseases can lead to the cessation of tooth development, resulting in compromised tooth structure and functions. Despite numerous efforts to induce pulp regeneration, effective strategies are still lacking. Growth factors (GFs) hold considerable promise in pulp regeneration due to their diverse cellular regulatory properties. However, the limited half-lives and susceptibility to degradation of exogenous GFs necessitate the administration of supra-physiological doses, leading to undesirable side effects. In this research, a heparin-functionalized bioactive glass (CaO–P2O5–SiO2–Heparin, abbreviated as PSC-Heparin) with strong bioactivity and a stable neutral pH is developed as a promising candidate to addressing challenges in pulp regeneration. Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis reveal the successful synthesis of PSC-Heparin. Scanning electron microscopy and X-ray diffraction show the hydroxyapatite formation can be observed on the surface of PSC-Heparin after soaking in simulated body fluid for 12 h. PSC-Heparin is capable of harvesting various endogenous GFs and sustainably releasing them over an extended duration by the enzyme-linked immunosorbent assay. Cytological experiments show that developed PSC-Heparin can facilitate the adhesion, migration, proliferation, and odontogenic differentiation of stem cells from apical papillae. Notably, the histological analysis of subcutaneous implantation in nude mice demonstrates PSC-Heparin is capable of promoting the odontoblast-like layers and pulp-dentin complex formation without the addition of exogenous GFs, which is vital for clinical applications. This work highlights an effective strategy of harvesting endogenous GFs and avoiding the involvement of exogenous GFs to achieve pulp-dentin complex regeneration, which may open a new horizon for regenerative endodontic therapy.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>38833722</pmid><doi>10.1021/acsami.4c03118</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-6320-0913</orcidid><orcidid>https://orcid.org/0000-0002-2287-6084</orcidid><orcidid>https://orcid.org/0000-0002-2749-7788</orcidid></addata></record> |
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subjects | Animals Biological and Medical Applications of Materials and Interfaces Cell Differentiation - drug effects Cell Proliferation - drug effects Dental Pulp - cytology Dental Pulp - drug effects Dental Pulp - metabolism Glass - chemistry Heparin - chemistry Heparin - pharmacology Humans Intercellular Signaling Peptides and Proteins - chemistry Intercellular Signaling Peptides and Proteins - pharmacology Mice Mice, Nude Regeneration - drug effects Stem Cells - cytology Stem Cells - drug effects Stem Cells - metabolism |
title | Heparin-Functionalized Bioactive Glass to Harvest Endogenous Growth Factors for Pulp Regeneration |
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