Photoelectrochemical immunoassay of cardiac troponin I based on ZnTCPP/CdIn2S4 type-II heterojunction and co-catalyzed precipitation biolabeling
CdIn 2 S 4 and zinc tetrakis(4-carboxyphenyl)porphyrin (ZnTCPP) were synthesized by hydrothermal method, and an organic dye-sensitized inorganic semiconductor ZnTCPP/CdIn 2 S 4 type II heterojunction was constructed on a fluorine-doped tin oxide (FTO) substrate electrode. A sandwich immunostructure...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2024-07, Vol.191 (7), p.364, Article 364 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Li, Jiahui Tang, Fengci Xie, Qingji Zeng, Xingyu He, Fang Xie, Qingji |
| description | CdIn
2
S
4
and zinc tetrakis(4-carboxyphenyl)porphyrin (ZnTCPP) were synthesized by hydrothermal method, and an organic dye-sensitized inorganic semiconductor ZnTCPP/CdIn
2
S
4
type II heterojunction was constructed on a fluorine-doped tin oxide (FTO) substrate electrode. A sandwich immunostructure for signal-attenuation photoelectrochemical (PEC) detection of cardiac troponin I (cTnI) was constructed using the ZnTCPP/CdIn
2
S
4
/FTO photoanode and a horseradish peroxidase (HRP)-ZnFe
2
O
4
-Ab
2
-bovine serum albumin (BSA) immunolabeling complex. The bioenzyme HRP and the HRP-like nanozyme ZnFe
2
O
4
can co-catalyze the oxidation of 4-chloro-1-naphthol (4-CN) by H
2
O
2
to produce an insoluble precipitate on the photoanode, thus notably reducing the anodic photocurrent for quantitative determination of cTnI. Under the optimal conditions, the photocurrent at 0 V vs. SCE in 0.1 M phosphate buffer solution (pH 7.40) containing 0.1 M ascorbic acid was linear with the logarithm of cTnI concentration from 500 fg mL
−1
to 50.0 ng mL
−1
, and the limit of detection (LOD,
S
/
N
= 3) is 0.15 pg mL
−1
. Spiked recoveries were 95.1% ~ 104% for assay of cTnI in human serum samples.
Graphical Abstract |
| doi_str_mv | 10.1007/s00604-024-06436-8 |
| format | Article |
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2
S
4
and zinc tetrakis(4-carboxyphenyl)porphyrin (ZnTCPP) were synthesized by hydrothermal method, and an organic dye-sensitized inorganic semiconductor ZnTCPP/CdIn
2
S
4
type II heterojunction was constructed on a fluorine-doped tin oxide (FTO) substrate electrode. A sandwich immunostructure for signal-attenuation photoelectrochemical (PEC) detection of cardiac troponin I (cTnI) was constructed using the ZnTCPP/CdIn
2
S
4
/FTO photoanode and a horseradish peroxidase (HRP)-ZnFe
2
O
4
-Ab
2
-bovine serum albumin (BSA) immunolabeling complex. The bioenzyme HRP and the HRP-like nanozyme ZnFe
2
O
4
can co-catalyze the oxidation of 4-chloro-1-naphthol (4-CN) by H
2
O
2
to produce an insoluble precipitate on the photoanode, thus notably reducing the anodic photocurrent for quantitative determination of cTnI. Under the optimal conditions, the photocurrent at 0 V vs. SCE in 0.1 M phosphate buffer solution (pH 7.40) containing 0.1 M ascorbic acid was linear with the logarithm of cTnI concentration from 500 fg mL
−1
to 50.0 ng mL
−1
, and the limit of detection (LOD,
S
/
N
= 3) is 0.15 pg mL
−1
. Spiked recoveries were 95.1% ~ 104% for assay of cTnI in human serum samples.
Graphical Abstract</description><identifier>ISSN: 0026-3672</identifier><identifier>ISSN: 1436-5073</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-024-06436-8</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Analytical Chemistry ; Ascorbic acid ; Buffer solutions ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Fluorine ; Heterojunctions ; Hydrogen peroxide ; Immunoassay ; Microengineering ; Nanochemistry ; Nanotechnology ; Original Paper ; Oxidation ; Peroxidase ; Photoanodes ; Photoelectric effect ; Photoelectric emission ; Porphyrins ; Serum albumin ; Substrates ; Tin oxides ; Zinc ferrites</subject><ispartof>Mikrochimica acta (1966), 2024-07, Vol.191 (7), p.364, Article 364</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1488-5c11a3bb8adca9b448623db56c8e7b69de38044b41fd4cb187da1cd304c52fce3</cites><orcidid>0000-0001-7860-446X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-024-06436-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-024-06436-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Li, Jiahui</creatorcontrib><creatorcontrib>Tang, Fengci</creatorcontrib><creatorcontrib>Xie, Qingji</creatorcontrib><creatorcontrib>Zeng, Xingyu</creatorcontrib><creatorcontrib>He, Fang</creatorcontrib><creatorcontrib>Xie, Qingji</creatorcontrib><title>Photoelectrochemical immunoassay of cardiac troponin I based on ZnTCPP/CdIn2S4 type-II heterojunction and co-catalyzed precipitation biolabeling</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><description>CdIn
2
S
4
and zinc tetrakis(4-carboxyphenyl)porphyrin (ZnTCPP) were synthesized by hydrothermal method, and an organic dye-sensitized inorganic semiconductor ZnTCPP/CdIn
2
S
4
type II heterojunction was constructed on a fluorine-doped tin oxide (FTO) substrate electrode. A sandwich immunostructure for signal-attenuation photoelectrochemical (PEC) detection of cardiac troponin I (cTnI) was constructed using the ZnTCPP/CdIn
2
S
4
/FTO photoanode and a horseradish peroxidase (HRP)-ZnFe
2
O
4
-Ab
2
-bovine serum albumin (BSA) immunolabeling complex. The bioenzyme HRP and the HRP-like nanozyme ZnFe
2
O
4
can co-catalyze the oxidation of 4-chloro-1-naphthol (4-CN) by H
2
O
2
to produce an insoluble precipitate on the photoanode, thus notably reducing the anodic photocurrent for quantitative determination of cTnI. Under the optimal conditions, the photocurrent at 0 V vs. SCE in 0.1 M phosphate buffer solution (pH 7.40) containing 0.1 M ascorbic acid was linear with the logarithm of cTnI concentration from 500 fg mL
−1
to 50.0 ng mL
−1
, and the limit of detection (LOD,
S
/
N
= 3) is 0.15 pg mL
−1
. Spiked recoveries were 95.1% ~ 104% for assay of cTnI in human serum samples.
Graphical Abstract</description><subject>Analytical Chemistry</subject><subject>Ascorbic acid</subject><subject>Buffer solutions</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Fluorine</subject><subject>Heterojunctions</subject><subject>Hydrogen peroxide</subject><subject>Immunoassay</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Oxidation</subject><subject>Peroxidase</subject><subject>Photoanodes</subject><subject>Photoelectric effect</subject><subject>Photoelectric emission</subject><subject>Porphyrins</subject><subject>Serum albumin</subject><subject>Substrates</subject><subject>Tin oxides</subject><subject>Zinc ferrites</subject><issn>0026-3672</issn><issn>1436-5073</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kU2LFDEQhoMoOO76BzwFvHjp3Xx1OnuUwY-GBQdcL15CUknvZOhO2qT7MP6K_clmZhYEDx6KKqjnfSnqRegdJTeUkO62ECKJaAirJQWXjXqBNvQ0tKTjL9GGECYbLjv2Gr0p5UAI7SQTG_S026cl-dHDkhPs_RTAjDhM0xqTKcUccRowmOyCAVyROcUQcY-tKd7hFPHP-LDd7W63ro_su8DLcfZN3-O9X3xOhzXCEiplosOQGjCLGY-_q3LOHsIcFnNe25BGY_0Y4uM1ejWYsfi3z_0K_fj86WH7tbn_9qXffrxvgAqlmhYoNdxaZRyYOyuEkow720pQvrPyznmuiBBW0MEJsFR1zlBwnAho2QCeX6EPF985p1-rL4ueQgE_jib6tBbN6x9bRRjtKvr-H_SQ1hzrdWeKKirJiWIXCnIqJftBzzlMJh81JfoUkr6EpGtI-hySVlXEL6JS4fjo81_r_6j-AOYOlvA</recordid><startdate>20240701</startdate><enddate>20240701</enddate><creator>Li, Jiahui</creator><creator>Tang, Fengci</creator><creator>Xie, Qingji</creator><creator>Zeng, Xingyu</creator><creator>He, Fang</creator><creator>Xie, Qingji</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7860-446X</orcidid></search><sort><creationdate>20240701</creationdate><title>Photoelectrochemical immunoassay of cardiac troponin I based on ZnTCPP/CdIn2S4 type-II heterojunction and co-catalyzed precipitation biolabeling</title><author>Li, Jiahui ; Tang, Fengci ; Xie, Qingji ; Zeng, Xingyu ; He, Fang ; Xie, Qingji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1488-5c11a3bb8adca9b448623db56c8e7b69de38044b41fd4cb187da1cd304c52fce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Analytical Chemistry</topic><topic>Ascorbic acid</topic><topic>Buffer solutions</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Fluorine</topic><topic>Heterojunctions</topic><topic>Hydrogen peroxide</topic><topic>Immunoassay</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Oxidation</topic><topic>Peroxidase</topic><topic>Photoanodes</topic><topic>Photoelectric effect</topic><topic>Photoelectric emission</topic><topic>Porphyrins</topic><topic>Serum albumin</topic><topic>Substrates</topic><topic>Tin oxides</topic><topic>Zinc ferrites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jiahui</creatorcontrib><creatorcontrib>Tang, Fengci</creatorcontrib><creatorcontrib>Xie, Qingji</creatorcontrib><creatorcontrib>Zeng, Xingyu</creatorcontrib><creatorcontrib>He, Fang</creatorcontrib><creatorcontrib>Xie, Qingji</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jiahui</au><au>Tang, Fengci</au><au>Xie, Qingji</au><au>Zeng, Xingyu</au><au>He, Fang</au><au>Xie, Qingji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photoelectrochemical immunoassay of cardiac troponin I based on ZnTCPP/CdIn2S4 type-II heterojunction and co-catalyzed precipitation biolabeling</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><date>2024-07-01</date><risdate>2024</risdate><volume>191</volume><issue>7</issue><spage>364</spage><pages>364-</pages><artnum>364</artnum><issn>0026-3672</issn><issn>1436-5073</issn><eissn>1436-5073</eissn><abstract>CdIn
2
S
4
and zinc tetrakis(4-carboxyphenyl)porphyrin (ZnTCPP) were synthesized by hydrothermal method, and an organic dye-sensitized inorganic semiconductor ZnTCPP/CdIn
2
S
4
type II heterojunction was constructed on a fluorine-doped tin oxide (FTO) substrate electrode. A sandwich immunostructure for signal-attenuation photoelectrochemical (PEC) detection of cardiac troponin I (cTnI) was constructed using the ZnTCPP/CdIn
2
S
4
/FTO photoanode and a horseradish peroxidase (HRP)-ZnFe
2
O
4
-Ab
2
-bovine serum albumin (BSA) immunolabeling complex. The bioenzyme HRP and the HRP-like nanozyme ZnFe
2
O
4
can co-catalyze the oxidation of 4-chloro-1-naphthol (4-CN) by H
2
O
2
to produce an insoluble precipitate on the photoanode, thus notably reducing the anodic photocurrent for quantitative determination of cTnI. Under the optimal conditions, the photocurrent at 0 V vs. SCE in 0.1 M phosphate buffer solution (pH 7.40) containing 0.1 M ascorbic acid was linear with the logarithm of cTnI concentration from 500 fg mL
−1
to 50.0 ng mL
−1
, and the limit of detection (LOD,
S
/
N
= 3) is 0.15 pg mL
−1
. Spiked recoveries were 95.1% ~ 104% for assay of cTnI in human serum samples.
Graphical Abstract</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><doi>10.1007/s00604-024-06436-8</doi><orcidid>https://orcid.org/0000-0001-7860-446X</orcidid></addata></record> |
| fulltext | fulltext |
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| language | eng |
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| source | SpringerNature Journals |
| subjects | Analytical Chemistry Ascorbic acid Buffer solutions Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Fluorine Heterojunctions Hydrogen peroxide Immunoassay Microengineering Nanochemistry Nanotechnology Original Paper Oxidation Peroxidase Photoanodes Photoelectric effect Photoelectric emission Porphyrins Serum albumin Substrates Tin oxides Zinc ferrites |
| title | Photoelectrochemical immunoassay of cardiac troponin I based on ZnTCPP/CdIn2S4 type-II heterojunction and co-catalyzed precipitation biolabeling |
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