Osteogenic potential of adipose stem cells on hydroxyapatite-functionalized decellularized amniotic membrane
Amniotic membrane (AM) is an attractive source for bone tissue engineering because of its low immunogenicity, contains biomolecules and proteins, and osteogenic differentiation properties. Hydroxyapatite is widely used as bone scaffolds due to its biocompatibility and bioactivity properties. The aim...
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| creator | Firouzeh, Arezoo Shabani, Iman Karimi-Soflou, Reza Shabani, Azadeh |
| description | Amniotic membrane (AM) is an attractive source for bone tissue engineering because of its low immunogenicity, contains biomolecules and proteins, and osteogenic differentiation properties. Hydroxyapatite is widely used as bone scaffolds due to its biocompatibility and bioactivity properties. The aim of this study is to design and fabricate scaffold based on hydroxyapatite-coated decellularized amniotic membrane (DAM-HA) for bone tissue engineering purpose. So human amniotic membranes were collected from healthy donors and decellularized (DAM). Then a hydroxyapatite-coating was created by immersion in 10X SBF, under variable parameters of pH and incubation time. Hydroxyapatite-coating was characterized and the optimal sample was selected. Human adipose-derived mesenchymal stem cell behaviors were assessed on control, amniotic membrane, and coated amniotic membrane. The results of the SEM, MTT assay, and Live-Dead staining showed that DAM and DAM-HA support cell adhesion, viability and proliferation. Osteogenic differentiation was evaluated by assessment of alkaline phosphatase activity and expression of osteogenic markers. Maximum gene expression values compared to control occurred in 14 days for alkalin phosphatase, while the highest values for osteocalcin and osteopontin in 21 days. These gene expression values in DAM and DAM-HA for alkalin phosphatase is 6.41 and 8.47, for osteocalcin is 3.95 and 5.94 and for osteopontin is 5.59 and 9.9 respectively. The results of this study indicated DAM supports the survival and growth of stem cells. Also, addition of hydroxyapatite component to DAM promotes osteogenic differentiation while maintaining viability. Therefore, hydroxyapatite-coated decellularized amniotic membrane can be a promising choice for bone tissue engineering applications.
[Display omitted]
•Amniotic membrane is a suitable source for bone tissue engineering application.•Prepared a homogeneous hydroxyapatite coating through immersion in 10X SBF.•Hydroxyapatite coated amniotic membrane promoted the viability of the cells.•Hydroxyapatite coated amniotic membrane promotes osteogenic differentiation. |
| doi_str_mv | 10.1016/j.colsurfb.2024.113974 |
| format | Article |
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[Display omitted]
•Amniotic membrane is a suitable source for bone tissue engineering application.•Prepared a homogeneous hydroxyapatite coating through immersion in 10X SBF.•Hydroxyapatite coated amniotic membrane promoted the viability of the cells.•Hydroxyapatite coated amniotic membrane promotes osteogenic differentiation.</description><identifier>ISSN: 0927-7765</identifier><identifier>ISSN: 1873-4367</identifier><identifier>EISSN: 1873-4367</identifier><identifier>DOI: 10.1016/j.colsurfb.2024.113974</identifier><identifier>PMID: 38810465</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>10X SBF ; Adipose Tissue - cytology ; Alkaline Phosphatase - metabolism ; Amnion - chemistry ; Amnion - cytology ; Amnion - metabolism ; Amniotic membrane ; Bone tissue engineering ; Cell Adhesion - drug effects ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Coated amniotic membrane ; Decellularization ; Durapatite - chemistry ; Durapatite - pharmacology ; Humans ; Hydroxyapatite ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - metabolism ; Osteogenesis - drug effects ; Stem Cells - cytology ; Stem Cells - metabolism ; Tissue Engineering ; Tissue Scaffolds - chemistry</subject><ispartof>Colloids and surfaces, B, Biointerfaces, 2024-08, Vol.240, p.113974, Article 113974</ispartof><rights>2024</rights><rights>Copyright © 2024. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c315t-99867f142cfedb7ea6e07d42e508c8956e755644dd49adb5b57991da377f7bbf3</cites><orcidid>0000-0002-9666-712X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.colsurfb.2024.113974$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38810465$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Firouzeh, Arezoo</creatorcontrib><creatorcontrib>Shabani, Iman</creatorcontrib><creatorcontrib>Karimi-Soflou, Reza</creatorcontrib><creatorcontrib>Shabani, Azadeh</creatorcontrib><title>Osteogenic potential of adipose stem cells on hydroxyapatite-functionalized decellularized amniotic membrane</title><title>Colloids and surfaces, B, Biointerfaces</title><addtitle>Colloids Surf B Biointerfaces</addtitle><description>Amniotic membrane (AM) is an attractive source for bone tissue engineering because of its low immunogenicity, contains biomolecules and proteins, and osteogenic differentiation properties. Hydroxyapatite is widely used as bone scaffolds due to its biocompatibility and bioactivity properties. The aim of this study is to design and fabricate scaffold based on hydroxyapatite-coated decellularized amniotic membrane (DAM-HA) for bone tissue engineering purpose. So human amniotic membranes were collected from healthy donors and decellularized (DAM). Then a hydroxyapatite-coating was created by immersion in 10X SBF, under variable parameters of pH and incubation time. Hydroxyapatite-coating was characterized and the optimal sample was selected. Human adipose-derived mesenchymal stem cell behaviors were assessed on control, amniotic membrane, and coated amniotic membrane. The results of the SEM, MTT assay, and Live-Dead staining showed that DAM and DAM-HA support cell adhesion, viability and proliferation. Osteogenic differentiation was evaluated by assessment of alkaline phosphatase activity and expression of osteogenic markers. Maximum gene expression values compared to control occurred in 14 days for alkalin phosphatase, while the highest values for osteocalcin and osteopontin in 21 days. These gene expression values in DAM and DAM-HA for alkalin phosphatase is 6.41 and 8.47, for osteocalcin is 3.95 and 5.94 and for osteopontin is 5.59 and 9.9 respectively. The results of this study indicated DAM supports the survival and growth of stem cells. Also, addition of hydroxyapatite component to DAM promotes osteogenic differentiation while maintaining viability. Therefore, hydroxyapatite-coated decellularized amniotic membrane can be a promising choice for bone tissue engineering applications.
[Display omitted]
•Amniotic membrane is a suitable source for bone tissue engineering application.•Prepared a homogeneous hydroxyapatite coating through immersion in 10X SBF.•Hydroxyapatite coated amniotic membrane promoted the viability of the cells.•Hydroxyapatite coated amniotic membrane promotes osteogenic differentiation.</description><subject>10X SBF</subject><subject>Adipose Tissue - cytology</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Amnion - chemistry</subject><subject>Amnion - cytology</subject><subject>Amnion - metabolism</subject><subject>Amniotic membrane</subject><subject>Bone tissue engineering</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Coated amniotic membrane</subject><subject>Decellularization</subject><subject>Durapatite - chemistry</subject><subject>Durapatite - pharmacology</subject><subject>Humans</subject><subject>Hydroxyapatite</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>Osteogenesis - drug effects</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds - chemistry</subject><issn>0927-7765</issn><issn>1873-4367</issn><issn>1873-4367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMlu2zAQQIkiReO6-YVAx1zkkuIm3RIE2YAAvrRngiKHKQ1JVEiqqPv1pWu7154Gg3mzPYSuCd4QTMTX3caEIS3R9ZsGN2xDCO0k-4BWpJW0ZlTIC7TCXSNrKQW_RJ9T2mFcSCI_oUvatgQzwVdo2KYM4Q0mb6o5ZJiy10MVXKWtn0OCqpTHysAwpCpM1Y-9jeHXXs86-wy1WyaTfZj04H-DrSwcwGXQ8W-qx8mHXAaPMPZRT_AFfXR6SHB1imv0_fHh2_1z_bp9erm_e60NJTzXXdcK6QhrjAPbS9ACsLSsAY5b03ZcgORcMGYt67Ttec9l1xGrqZRO9r2ja3RznDvH8L5Aymr06XBauSEsSVEsGk6bYqeg4oiaGFKK4NQc_ajjXhGsDqbVTp1Nq4NpdTRdGq9PO5Z-BPuv7ay2ALdHAMqnPz1ElYyHyYD1EUxWNvj_7fgD3VyWJg</recordid><startdate>202408</startdate><enddate>202408</enddate><creator>Firouzeh, Arezoo</creator><creator>Shabani, Iman</creator><creator>Karimi-Soflou, Reza</creator><creator>Shabani, Azadeh</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9666-712X</orcidid></search><sort><creationdate>202408</creationdate><title>Osteogenic potential of adipose stem cells on hydroxyapatite-functionalized decellularized amniotic membrane</title><author>Firouzeh, Arezoo ; Shabani, Iman ; Karimi-Soflou, Reza ; Shabani, Azadeh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-99867f142cfedb7ea6e07d42e508c8956e755644dd49adb5b57991da377f7bbf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>10X SBF</topic><topic>Adipose Tissue - cytology</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Amnion - chemistry</topic><topic>Amnion - cytology</topic><topic>Amnion - metabolism</topic><topic>Amniotic membrane</topic><topic>Bone tissue engineering</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Coated amniotic membrane</topic><topic>Decellularization</topic><topic>Durapatite - chemistry</topic><topic>Durapatite - pharmacology</topic><topic>Humans</topic><topic>Hydroxyapatite</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>Osteogenesis - drug effects</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Tissue Engineering</topic><topic>Tissue Scaffolds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Firouzeh, Arezoo</creatorcontrib><creatorcontrib>Shabani, Iman</creatorcontrib><creatorcontrib>Karimi-Soflou, Reza</creatorcontrib><creatorcontrib>Shabani, Azadeh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Firouzeh, Arezoo</au><au>Shabani, Iman</au><au>Karimi-Soflou, Reza</au><au>Shabani, Azadeh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osteogenic potential of adipose stem cells on hydroxyapatite-functionalized decellularized amniotic membrane</atitle><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle><addtitle>Colloids Surf B Biointerfaces</addtitle><date>2024-08</date><risdate>2024</risdate><volume>240</volume><spage>113974</spage><pages>113974-</pages><artnum>113974</artnum><issn>0927-7765</issn><issn>1873-4367</issn><eissn>1873-4367</eissn><abstract>Amniotic membrane (AM) is an attractive source for bone tissue engineering because of its low immunogenicity, contains biomolecules and proteins, and osteogenic differentiation properties. Hydroxyapatite is widely used as bone scaffolds due to its biocompatibility and bioactivity properties. The aim of this study is to design and fabricate scaffold based on hydroxyapatite-coated decellularized amniotic membrane (DAM-HA) for bone tissue engineering purpose. So human amniotic membranes were collected from healthy donors and decellularized (DAM). Then a hydroxyapatite-coating was created by immersion in 10X SBF, under variable parameters of pH and incubation time. Hydroxyapatite-coating was characterized and the optimal sample was selected. Human adipose-derived mesenchymal stem cell behaviors were assessed on control, amniotic membrane, and coated amniotic membrane. The results of the SEM, MTT assay, and Live-Dead staining showed that DAM and DAM-HA support cell adhesion, viability and proliferation. Osteogenic differentiation was evaluated by assessment of alkaline phosphatase activity and expression of osteogenic markers. Maximum gene expression values compared to control occurred in 14 days for alkalin phosphatase, while the highest values for osteocalcin and osteopontin in 21 days. These gene expression values in DAM and DAM-HA for alkalin phosphatase is 6.41 and 8.47, for osteocalcin is 3.95 and 5.94 and for osteopontin is 5.59 and 9.9 respectively. The results of this study indicated DAM supports the survival and growth of stem cells. Also, addition of hydroxyapatite component to DAM promotes osteogenic differentiation while maintaining viability. Therefore, hydroxyapatite-coated decellularized amniotic membrane can be a promising choice for bone tissue engineering applications.
[Display omitted]
•Amniotic membrane is a suitable source for bone tissue engineering application.•Prepared a homogeneous hydroxyapatite coating through immersion in 10X SBF.•Hydroxyapatite coated amniotic membrane promoted the viability of the cells.•Hydroxyapatite coated amniotic membrane promotes osteogenic differentiation.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>38810465</pmid><doi>10.1016/j.colsurfb.2024.113974</doi><orcidid>https://orcid.org/0000-0002-9666-712X</orcidid></addata></record> |
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| subjects | 10X SBF Adipose Tissue - cytology Alkaline Phosphatase - metabolism Amnion - chemistry Amnion - cytology Amnion - metabolism Amniotic membrane Bone tissue engineering Cell Adhesion - drug effects Cell Differentiation - drug effects Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured Coated amniotic membrane Decellularization Durapatite - chemistry Durapatite - pharmacology Humans Hydroxyapatite Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - metabolism Osteogenesis - drug effects Stem Cells - cytology Stem Cells - metabolism Tissue Engineering Tissue Scaffolds - chemistry |
| title | Osteogenic potential of adipose stem cells on hydroxyapatite-functionalized decellularized amniotic membrane |
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