A three-dimensional cell culture approach to investigate cytotoxic effects and production of inflammatory mediators by epoxy resin-based and calcium silicate-based endodontic sealer
Objectives The aim of the present study was to assess the cytocompatibility of epoxy resin-based AH Plus Jet (Dentsply De Trey, Konstanz, Germany), Sealer Plus (MK Life, Porto Alegre, Brazil), calcium silicate-based Bio-C Sealer (Angelus, Londrina, PR, Brazil), Sealer Plus BC (MK Life) and AH Plus B...
Gespeichert in:
| Veröffentlicht in: | Clinical oral investigations 2024-05, Vol.28 (6), p.344, Article 344 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 6 |
| container_start_page | 344 |
| container_title | Clinical oral investigations |
| container_volume | 28 |
| creator | Scelza, Miriam F.Z. Tavares, Sandro J.O. Scelza, Pantaleo Ramos, Gabriel S. Lima Aboud, Lilian Rachel de Piasecki, Lucila Leite, Paulo Emílio C. Silva, Jéssica Dornelas da Soares-Lima, Sheila Coelho Alves, Gutemberg G. |
| description | Objectives
The aim of the present study was to assess the cytocompatibility of epoxy resin-based AH Plus Jet (Dentsply De Trey, Konstanz, Germany), Sealer Plus (MK Life, Porto Alegre, Brazil), calcium silicate-based Bio-C Sealer (Angelus, Londrina, PR, Brazil), Sealer Plus BC (MK Life) and AH Plus BC (Dentsply) through a tridimensional (3D) culture model of human osteoblast-like cells.
Methods
Spheroids of MG-63 cells were produced and exposed to fresh root canal sealers extracts by 24 h, and the cytotoxicity was assessed by the Lactate Dehydrogenase assay (LDH). The distribution of dead cells within the microtissue was assessed by fluorescence microscopy, and morphological effects were investigated by histological analysis. The secreted inflammatory mediators were detected in cell supernatants through flow luminometry (XMap Luminex).
Results
Cells incubated with AH Plus Jet, AH Plus BC, Sealer Plus BC and Bio-C Sealer extracts showed high rates of cell viability, while the Sealer Plus induced a significant reduction of cell viability, causing reduction on the spheroid structure. Sealer Plus and Seaker Plus BC caused alterations on 3D microtissue morphology. The AH Plus BC extract was associated with the downregulation of secretion of pro-inflammatory cytokines IL-5, IL-7, IP-10 and RANTES.
Conclusions
The new AH Plus BC calcium silicate-based endodontic sealer did not reduce cell viability in vitro, while led to the downregulation of pro-inflammatory cytokines.
Clinical significance
Choosing the appropriate endodontic sealer is a crucial step. AH Plus BC demonstrated high cell viability and downregulation of pro-inflammatory cytokines, appearing reliable for clinical use, while Sealer Plus presented lower cytocompatibility. |
| doi_str_mv | 10.1007/s00784-024-05743-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3061784215</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3061784215</sourcerecordid><originalsourceid>FETCH-LOGICAL-c256t-bcb93d7d64b627529b7297d02e59c7276f1dcb9211586c9628a04f5ad838c3a23</originalsourceid><addsrcrecordid>eNp9kctu1TAQhiNERUvLC7BAltiwCfUtdrKsqnKRKrGha8uxJ62rJD7YDjp5MN6POc3hIhYsPB5pvvlHM39VvWb0PaNUX2YMrawpx9doKer9s-qMSaFqoTV7_ld-Wr3M-ZFSJpUWL6pT0ba0k1KeVT-uSHlIALUPE8w5xNmOxMGIYRnLkoDY3S5F6x5IiSTM3yGXcG8LELeWWOI-OALDAK5kYmdPkPWLK6hD4oD8MNppsiWmlUzgwyHLpF8J7OJ-JQlymOveZvBP3c6OLiwTyWEMDoccSzD76ONccFYGO0K6qE4GO2Z4dfzPq7sPN1-vP9W3Xz5-vr66rR1vVKl713fCa69kr7hueNdr3mlPOTSd01yrgXlEOGNNq1yneGupHBrrW9E6Ybk4r95turjWtwVXN1PIh-vYGeKSjaCKoQWcNYi-_Qd9jEvCa25Ug461FCm-US7FnBMMZpfCZNNqGDUHU81mqkFTzZOpZo9Nb47SS49H_N3yy0UExAZkLM33kP7M_o_sT1VnsZA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3061510080</pqid></control><display><type>article</type><title>A three-dimensional cell culture approach to investigate cytotoxic effects and production of inflammatory mediators by epoxy resin-based and calcium silicate-based endodontic sealer</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Scelza, Miriam F.Z. ; Tavares, Sandro J.O. ; Scelza, Pantaleo ; Ramos, Gabriel S. ; Lima Aboud, Lilian Rachel de ; Piasecki, Lucila ; Leite, Paulo Emílio C. ; Silva, Jéssica Dornelas da ; Soares-Lima, Sheila Coelho ; Alves, Gutemberg G.</creator><creatorcontrib>Scelza, Miriam F.Z. ; Tavares, Sandro J.O. ; Scelza, Pantaleo ; Ramos, Gabriel S. ; Lima Aboud, Lilian Rachel de ; Piasecki, Lucila ; Leite, Paulo Emílio C. ; Silva, Jéssica Dornelas da ; Soares-Lima, Sheila Coelho ; Alves, Gutemberg G.</creatorcontrib><description>Objectives
The aim of the present study was to assess the cytocompatibility of epoxy resin-based AH Plus Jet (Dentsply De Trey, Konstanz, Germany), Sealer Plus (MK Life, Porto Alegre, Brazil), calcium silicate-based Bio-C Sealer (Angelus, Londrina, PR, Brazil), Sealer Plus BC (MK Life) and AH Plus BC (Dentsply) through a tridimensional (3D) culture model of human osteoblast-like cells.
Methods
Spheroids of MG-63 cells were produced and exposed to fresh root canal sealers extracts by 24 h, and the cytotoxicity was assessed by the Lactate Dehydrogenase assay (LDH). The distribution of dead cells within the microtissue was assessed by fluorescence microscopy, and morphological effects were investigated by histological analysis. The secreted inflammatory mediators were detected in cell supernatants through flow luminometry (XMap Luminex).
Results
Cells incubated with AH Plus Jet, AH Plus BC, Sealer Plus BC and Bio-C Sealer extracts showed high rates of cell viability, while the Sealer Plus induced a significant reduction of cell viability, causing reduction on the spheroid structure. Sealer Plus and Seaker Plus BC caused alterations on 3D microtissue morphology. The AH Plus BC extract was associated with the downregulation of secretion of pro-inflammatory cytokines IL-5, IL-7, IP-10 and RANTES.
Conclusions
The new AH Plus BC calcium silicate-based endodontic sealer did not reduce cell viability in vitro, while led to the downregulation of pro-inflammatory cytokines.
Clinical significance
Choosing the appropriate endodontic sealer is a crucial step. AH Plus BC demonstrated high cell viability and downregulation of pro-inflammatory cytokines, appearing reliable for clinical use, while Sealer Plus presented lower cytocompatibility.</description><identifier>ISSN: 1436-3771</identifier><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-024-05743-x</identifier><identifier>PMID: 38809444</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Calcium ; Calcium Compounds - pharmacology ; Cell culture ; Cell Culture Techniques, Three Dimensional - methods ; Cell Survival - drug effects ; Cell viability ; Cytokines ; Cytotoxicity ; Dentistry ; Down-regulation ; Endodontics ; Epoxy Resins ; Fluorescence microscopy ; Humans ; Inflammation ; Inflammation Mediators - metabolism ; IP-10 protein ; L-Lactate dehydrogenase ; Materials Testing ; Medicine ; Microscopy, Fluorescence ; Osteoblasts - drug effects ; RANTES ; Root Canal Filling Materials - pharmacology ; Root canals ; Sealing compounds ; Silicates - pharmacology ; Spheroids</subject><ispartof>Clinical oral investigations, 2024-05, Vol.28 (6), p.344, Article 344</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c256t-bcb93d7d64b627529b7297d02e59c7276f1dcb9211586c9628a04f5ad838c3a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-024-05743-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-024-05743-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38809444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Scelza, Miriam F.Z.</creatorcontrib><creatorcontrib>Tavares, Sandro J.O.</creatorcontrib><creatorcontrib>Scelza, Pantaleo</creatorcontrib><creatorcontrib>Ramos, Gabriel S.</creatorcontrib><creatorcontrib>Lima Aboud, Lilian Rachel de</creatorcontrib><creatorcontrib>Piasecki, Lucila</creatorcontrib><creatorcontrib>Leite, Paulo Emílio C.</creatorcontrib><creatorcontrib>Silva, Jéssica Dornelas da</creatorcontrib><creatorcontrib>Soares-Lima, Sheila Coelho</creatorcontrib><creatorcontrib>Alves, Gutemberg G.</creatorcontrib><title>A three-dimensional cell culture approach to investigate cytotoxic effects and production of inflammatory mediators by epoxy resin-based and calcium silicate-based endodontic sealer</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
The aim of the present study was to assess the cytocompatibility of epoxy resin-based AH Plus Jet (Dentsply De Trey, Konstanz, Germany), Sealer Plus (MK Life, Porto Alegre, Brazil), calcium silicate-based Bio-C Sealer (Angelus, Londrina, PR, Brazil), Sealer Plus BC (MK Life) and AH Plus BC (Dentsply) through a tridimensional (3D) culture model of human osteoblast-like cells.
Methods
Spheroids of MG-63 cells were produced and exposed to fresh root canal sealers extracts by 24 h, and the cytotoxicity was assessed by the Lactate Dehydrogenase assay (LDH). The distribution of dead cells within the microtissue was assessed by fluorescence microscopy, and morphological effects were investigated by histological analysis. The secreted inflammatory mediators were detected in cell supernatants through flow luminometry (XMap Luminex).
Results
Cells incubated with AH Plus Jet, AH Plus BC, Sealer Plus BC and Bio-C Sealer extracts showed high rates of cell viability, while the Sealer Plus induced a significant reduction of cell viability, causing reduction on the spheroid structure. Sealer Plus and Seaker Plus BC caused alterations on 3D microtissue morphology. The AH Plus BC extract was associated with the downregulation of secretion of pro-inflammatory cytokines IL-5, IL-7, IP-10 and RANTES.
Conclusions
The new AH Plus BC calcium silicate-based endodontic sealer did not reduce cell viability in vitro, while led to the downregulation of pro-inflammatory cytokines.
Clinical significance
Choosing the appropriate endodontic sealer is a crucial step. AH Plus BC demonstrated high cell viability and downregulation of pro-inflammatory cytokines, appearing reliable for clinical use, while Sealer Plus presented lower cytocompatibility.</description><subject>Calcium</subject><subject>Calcium Compounds - pharmacology</subject><subject>Cell culture</subject><subject>Cell Culture Techniques, Three Dimensional - methods</subject><subject>Cell Survival - drug effects</subject><subject>Cell viability</subject><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>Dentistry</subject><subject>Down-regulation</subject><subject>Endodontics</subject><subject>Epoxy Resins</subject><subject>Fluorescence microscopy</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Inflammation Mediators - metabolism</subject><subject>IP-10 protein</subject><subject>L-Lactate dehydrogenase</subject><subject>Materials Testing</subject><subject>Medicine</subject><subject>Microscopy, Fluorescence</subject><subject>Osteoblasts - drug effects</subject><subject>RANTES</subject><subject>Root Canal Filling Materials - pharmacology</subject><subject>Root canals</subject><subject>Sealing compounds</subject><subject>Silicates - pharmacology</subject><subject>Spheroids</subject><issn>1436-3771</issn><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1TAQhiNERUvLC7BAltiwCfUtdrKsqnKRKrGha8uxJ62rJD7YDjp5MN6POc3hIhYsPB5pvvlHM39VvWb0PaNUX2YMrawpx9doKer9s-qMSaFqoTV7_ld-Wr3M-ZFSJpUWL6pT0ba0k1KeVT-uSHlIALUPE8w5xNmOxMGIYRnLkoDY3S5F6x5IiSTM3yGXcG8LELeWWOI-OALDAK5kYmdPkPWLK6hD4oD8MNppsiWmlUzgwyHLpF8J7OJ-JQlymOveZvBP3c6OLiwTyWEMDoccSzD76ONccFYGO0K6qE4GO2Z4dfzPq7sPN1-vP9W3Xz5-vr66rR1vVKl713fCa69kr7hueNdr3mlPOTSd01yrgXlEOGNNq1yneGupHBrrW9E6Ybk4r95turjWtwVXN1PIh-vYGeKSjaCKoQWcNYi-_Qd9jEvCa25Ug461FCm-US7FnBMMZpfCZNNqGDUHU81mqkFTzZOpZo9Nb47SS49H_N3yy0UExAZkLM33kP7M_o_sT1VnsZA</recordid><startdate>20240529</startdate><enddate>20240529</enddate><creator>Scelza, Miriam F.Z.</creator><creator>Tavares, Sandro J.O.</creator><creator>Scelza, Pantaleo</creator><creator>Ramos, Gabriel S.</creator><creator>Lima Aboud, Lilian Rachel de</creator><creator>Piasecki, Lucila</creator><creator>Leite, Paulo Emílio C.</creator><creator>Silva, Jéssica Dornelas da</creator><creator>Soares-Lima, Sheila Coelho</creator><creator>Alves, Gutemberg G.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20240529</creationdate><title>A three-dimensional cell culture approach to investigate cytotoxic effects and production of inflammatory mediators by epoxy resin-based and calcium silicate-based endodontic sealer</title><author>Scelza, Miriam F.Z. ; Tavares, Sandro J.O. ; Scelza, Pantaleo ; Ramos, Gabriel S. ; Lima Aboud, Lilian Rachel de ; Piasecki, Lucila ; Leite, Paulo Emílio C. ; Silva, Jéssica Dornelas da ; Soares-Lima, Sheila Coelho ; Alves, Gutemberg G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-bcb93d7d64b627529b7297d02e59c7276f1dcb9211586c9628a04f5ad838c3a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Calcium</topic><topic>Calcium Compounds - pharmacology</topic><topic>Cell culture</topic><topic>Cell Culture Techniques, Three Dimensional - methods</topic><topic>Cell Survival - drug effects</topic><topic>Cell viability</topic><topic>Cytokines</topic><topic>Cytotoxicity</topic><topic>Dentistry</topic><topic>Down-regulation</topic><topic>Endodontics</topic><topic>Epoxy Resins</topic><topic>Fluorescence microscopy</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Inflammation Mediators - metabolism</topic><topic>IP-10 protein</topic><topic>L-Lactate dehydrogenase</topic><topic>Materials Testing</topic><topic>Medicine</topic><topic>Microscopy, Fluorescence</topic><topic>Osteoblasts - drug effects</topic><topic>RANTES</topic><topic>Root Canal Filling Materials - pharmacology</topic><topic>Root canals</topic><topic>Sealing compounds</topic><topic>Silicates - pharmacology</topic><topic>Spheroids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scelza, Miriam F.Z.</creatorcontrib><creatorcontrib>Tavares, Sandro J.O.</creatorcontrib><creatorcontrib>Scelza, Pantaleo</creatorcontrib><creatorcontrib>Ramos, Gabriel S.</creatorcontrib><creatorcontrib>Lima Aboud, Lilian Rachel de</creatorcontrib><creatorcontrib>Piasecki, Lucila</creatorcontrib><creatorcontrib>Leite, Paulo Emílio C.</creatorcontrib><creatorcontrib>Silva, Jéssica Dornelas da</creatorcontrib><creatorcontrib>Soares-Lima, Sheila Coelho</creatorcontrib><creatorcontrib>Alves, Gutemberg G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scelza, Miriam F.Z.</au><au>Tavares, Sandro J.O.</au><au>Scelza, Pantaleo</au><au>Ramos, Gabriel S.</au><au>Lima Aboud, Lilian Rachel de</au><au>Piasecki, Lucila</au><au>Leite, Paulo Emílio C.</au><au>Silva, Jéssica Dornelas da</au><au>Soares-Lima, Sheila Coelho</au><au>Alves, Gutemberg G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A three-dimensional cell culture approach to investigate cytotoxic effects and production of inflammatory mediators by epoxy resin-based and calcium silicate-based endodontic sealer</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2024-05-29</date><risdate>2024</risdate><volume>28</volume><issue>6</issue><spage>344</spage><pages>344-</pages><artnum>344</artnum><issn>1436-3771</issn><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
The aim of the present study was to assess the cytocompatibility of epoxy resin-based AH Plus Jet (Dentsply De Trey, Konstanz, Germany), Sealer Plus (MK Life, Porto Alegre, Brazil), calcium silicate-based Bio-C Sealer (Angelus, Londrina, PR, Brazil), Sealer Plus BC (MK Life) and AH Plus BC (Dentsply) through a tridimensional (3D) culture model of human osteoblast-like cells.
Methods
Spheroids of MG-63 cells were produced and exposed to fresh root canal sealers extracts by 24 h, and the cytotoxicity was assessed by the Lactate Dehydrogenase assay (LDH). The distribution of dead cells within the microtissue was assessed by fluorescence microscopy, and morphological effects were investigated by histological analysis. The secreted inflammatory mediators were detected in cell supernatants through flow luminometry (XMap Luminex).
Results
Cells incubated with AH Plus Jet, AH Plus BC, Sealer Plus BC and Bio-C Sealer extracts showed high rates of cell viability, while the Sealer Plus induced a significant reduction of cell viability, causing reduction on the spheroid structure. Sealer Plus and Seaker Plus BC caused alterations on 3D microtissue morphology. The AH Plus BC extract was associated with the downregulation of secretion of pro-inflammatory cytokines IL-5, IL-7, IP-10 and RANTES.
Conclusions
The new AH Plus BC calcium silicate-based endodontic sealer did not reduce cell viability in vitro, while led to the downregulation of pro-inflammatory cytokines.
Clinical significance
Choosing the appropriate endodontic sealer is a crucial step. AH Plus BC demonstrated high cell viability and downregulation of pro-inflammatory cytokines, appearing reliable for clinical use, while Sealer Plus presented lower cytocompatibility.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38809444</pmid><doi>10.1007/s00784-024-05743-x</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1436-3771 |
| ispartof | Clinical oral investigations, 2024-05, Vol.28 (6), p.344, Article 344 |
| issn | 1436-3771 1432-6981 1436-3771 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3061784215 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Calcium Calcium Compounds - pharmacology Cell culture Cell Culture Techniques, Three Dimensional - methods Cell Survival - drug effects Cell viability Cytokines Cytotoxicity Dentistry Down-regulation Endodontics Epoxy Resins Fluorescence microscopy Humans Inflammation Inflammation Mediators - metabolism IP-10 protein L-Lactate dehydrogenase Materials Testing Medicine Microscopy, Fluorescence Osteoblasts - drug effects RANTES Root Canal Filling Materials - pharmacology Root canals Sealing compounds Silicates - pharmacology Spheroids |
| title | A three-dimensional cell culture approach to investigate cytotoxic effects and production of inflammatory mediators by epoxy resin-based and calcium silicate-based endodontic sealer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T13%3A30%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20three-dimensional%20cell%20culture%20approach%20to%20investigate%20cytotoxic%20effects%20and%20production%20of%20inflammatory%20mediators%20by%20epoxy%20resin-based%20and%20calcium%20silicate-based%20endodontic%20sealer&rft.jtitle=Clinical%20oral%20investigations&rft.au=Scelza,%20Miriam%20F.Z.&rft.date=2024-05-29&rft.volume=28&rft.issue=6&rft.spage=344&rft.pages=344-&rft.artnum=344&rft.issn=1436-3771&rft.eissn=1436-3771&rft_id=info:doi/10.1007/s00784-024-05743-x&rft_dat=%3Cproquest_cross%3E3061784215%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3061510080&rft_id=info:pmid/38809444&rfr_iscdi=true |