Microbiota-induced inflammatory responses in bladder tumors promote epithelial-mesenchymal transition and enhanced immune infiltration

The intratumoral microbiota can modulate the tumor immune microenvironment (TIME); however, the underlying mechanism by which intratumoral microbiota influences the TIME in urothelial carcinoma of the bladder (UCB) remains unclear. To address this, we collected samples from 402 patients with UCB, in...

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Veröffentlicht in:Physiological genomics 2024-08, Vol.56 (8), p.544-554
Hauptverfasser: Li, Qiang, Sun, Yichao, Zhai, Kun, Geng, Bingzhi, Dong, Zhenkun, Ji, Lei, Chen, Hui, Cui, Yan
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container_end_page 554
container_issue 8
container_start_page 544
container_title Physiological genomics
container_volume 56
creator Li, Qiang
Sun, Yichao
Zhai, Kun
Geng, Bingzhi
Dong, Zhenkun
Ji, Lei
Chen, Hui
Cui, Yan
description The intratumoral microbiota can modulate the tumor immune microenvironment (TIME); however, the underlying mechanism by which intratumoral microbiota influences the TIME in urothelial carcinoma of the bladder (UCB) remains unclear. To address this, we collected samples from 402 patients with UCB, including paired host transcriptome and tumor microbiome data, from The Cancer Genome Atlas (TCGA). We found that the intratumoral microbiome profiles were significantly correlated with the expression pattern of epithelial-mesenchymal transition (EMT)-related genes. Furthermore, we detected that the genera and in tumors could indirectly promote the EMT program by inducing an inflammatory response. Moreover, the inflammatory response induced by these two intratumoral bacteria further enhanced intratumoral immune infiltration, affecting patient survival and response to immunotherapy. In addition, an independent immunotherapy cohort of 348 patients with bladder cancer was used to validate our results. Collectively, our study elucidates the potential mechanism by which the intratumoral microbiota influences the TIME of UCB and provides a new guiding strategy for the targeted therapy of UCB. The intratumoral microbiota may mediate the bladder tumor inflammatory response, thereby promoting the epithelial-mesenchymal transition program and influencing tumor immune infiltration.
doi_str_mv 10.1152/physiolgenomics.00032.2024
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subjects Bladder
Clostridiales - genetics
Epithelial-Mesenchymal Transition - genetics
Female
Gene Expression Regulation, Neoplastic
Humans
Immunotherapy
Immunotherapy - methods
Infiltration
Inflammation
Inflammation - immunology
Inflammation - microbiology
Male
Metastases
Microbiomes
Microbiota
Microenvironments
Middle Aged
Transcriptome - genetics
Transcriptomes
Tumor Microenvironment - immunology
Tumors
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - immunology
Urinary Bladder Neoplasms - microbiology
Urinary Bladder Neoplasms - pathology
Urothelial carcinoma
title Microbiota-induced inflammatory responses in bladder tumors promote epithelial-mesenchymal transition and enhanced immune infiltration
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