Sperm melatonin receptors, seminal plasma melatonin and semen freezability in goats
Goat bucks are seasonal breeders that show variation in sperm quality, endogenous melatonin (MLT), and presumably in the expression of MLT receptors on the sperm throughout the year, which may modify sperm freezability. The aim of this study was to determine whether sperm freezability is associated...
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| Veröffentlicht in: | Theriogenology 2024-09, Vol.225, p.98-106 |
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| creator | Cardenas-Padilla, Alberto J. Jimenez-Trejo, Francisco Cerbon, Marco Chavez-Garcia, Araceli Cruz-Cano, Norma B. Martinez-Torres, Martin Alcantar-Rodriguez, Alicia Medrano, Alfredo |
| description | Goat bucks are seasonal breeders that show variation in sperm quality, endogenous melatonin (MLT), and presumably in the expression of MLT receptors on the sperm throughout the year, which may modify sperm freezability. The aim of this study was to determine whether sperm freezability is associated with (i) endogenous melatonin levels in seminal plasma and (ii) the expression of sperm plasma membrane melatonin receptors (MT1, MT2). To evaluate this, spermatozoa from seven Saanen goat bucks were cryopreserved throughout the year in Mexico using a standard freezing protocol. Seminal plasma MLT concentrations were determined by ELISA and the expression and localization of MT1 and MT2 were detected by immunocytochemistry and confirmed by western blotting. The recovery rate of progressive motility after thawing was higher in spring than autumn and winter; in contrast, the F pattern (CTC assay) was higher in winter than in the other seasons. A proportional increase in the AR pattern (CTC assay) was smaller in winter than in the other seasons and the proportion of sperm showing high plasma membrane fluidity was higher in spring than in summer and autumn. The seminal plasma MLT concentrations showed no significant interseasonal differences. The MT1 receptor was immunolocalised at the apical region of the sperm head, while MT2 was mainly localised in the neck. The relative expression of MLT receptors showed significant differences between summer and winter for all bands, except at 75 kDa of MT2. In conclusion, there was an association between the relative expression of MT1 and MT2 receptors throughout the year and sperm freezability in goat bucks in México. Post-thaw sperm quality is enhanced in semen samples collected during breeding season.
•MLT concentration in goats' seminal plasma does not show interseasonal variation.•Expression of MLT receptors in goat spermatozoa shows variation throughout the year.•Goat buck sperm freezability is associated with the expression of MLT receptors. |
| doi_str_mv | 10.1016/j.theriogenology.2024.05.034 |
| format | Article |
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•MLT concentration in goats' seminal plasma does not show interseasonal variation.•Expression of MLT receptors in goat spermatozoa shows variation throughout the year.•Goat buck sperm freezability is associated with the expression of MLT receptors.</description><subject>Caprine semen</subject><subject>ELISA</subject><subject>Immunofluorescence</subject><subject>Seasonality</subject><subject>Western blotting</subject><issn>0093-691X</issn><issn>1879-3231</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqNkE1P4zAQhi20CMrHX0A5cNgDCWO7cWyJywrxJSFxKEjcLNeedF0lcbBTpPLrSdUuYm-c5vA-74zmIeScQkGBistlMfzF6MMCu9CExbpgwKYFlAXw6R6ZUFmpnDNOf5EJgOK5UPT1kByltAQALgQ9IIdcSqCVohMym_UY26zFxgyh810W0WI_hJgusoSt70yT9Y1JrfnGmM5tQuyyOiJ-mLlv_LDOxmQRzJBOyH5tmoSnu3lMXm5vnq_v88enu4frP4-55awaclu5kleKMVsKEFIglUoYxZTEyrma0pqhAOvmTIppJUt0SjKl7MhaqRTnx-T3dm8fw9sK06Bbnyw2jekwrJLmICjlcgp0RK-2qI0hpYi17qNvTVxrCnqjVS_1_1r1RquGUo9ax_rZ7tJq3qL7Kv_zOAK3WwDHf989Rp2sx86i86PQQbvgf3bpE91Okmk</recordid><startdate>20240901</startdate><enddate>20240901</enddate><creator>Cardenas-Padilla, Alberto J.</creator><creator>Jimenez-Trejo, Francisco</creator><creator>Cerbon, Marco</creator><creator>Chavez-Garcia, Araceli</creator><creator>Cruz-Cano, Norma B.</creator><creator>Martinez-Torres, Martin</creator><creator>Alcantar-Rodriguez, Alicia</creator><creator>Medrano, Alfredo</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0004-8750-8399</orcidid></search><sort><creationdate>20240901</creationdate><title>Sperm melatonin receptors, seminal plasma melatonin and semen freezability in goats</title><author>Cardenas-Padilla, Alberto J. ; Jimenez-Trejo, Francisco ; Cerbon, Marco ; Chavez-Garcia, Araceli ; Cruz-Cano, Norma B. ; Martinez-Torres, Martin ; Alcantar-Rodriguez, Alicia ; Medrano, Alfredo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c327t-c7d537922c560686e1896a9298e7ddf11f2e60cdb2864785ed98299c068c89933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Caprine semen</topic><topic>ELISA</topic><topic>Immunofluorescence</topic><topic>Seasonality</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cardenas-Padilla, Alberto J.</creatorcontrib><creatorcontrib>Jimenez-Trejo, Francisco</creatorcontrib><creatorcontrib>Cerbon, Marco</creatorcontrib><creatorcontrib>Chavez-Garcia, Araceli</creatorcontrib><creatorcontrib>Cruz-Cano, Norma B.</creatorcontrib><creatorcontrib>Martinez-Torres, Martin</creatorcontrib><creatorcontrib>Alcantar-Rodriguez, Alicia</creatorcontrib><creatorcontrib>Medrano, Alfredo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cardenas-Padilla, Alberto J.</au><au>Jimenez-Trejo, Francisco</au><au>Cerbon, Marco</au><au>Chavez-Garcia, Araceli</au><au>Cruz-Cano, Norma B.</au><au>Martinez-Torres, Martin</au><au>Alcantar-Rodriguez, Alicia</au><au>Medrano, Alfredo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sperm melatonin receptors, seminal plasma melatonin and semen freezability in goats</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2024-09-01</date><risdate>2024</risdate><volume>225</volume><spage>98</spage><epage>106</epage><pages>98-106</pages><issn>0093-691X</issn><issn>1879-3231</issn><eissn>1879-3231</eissn><abstract>Goat bucks are seasonal breeders that show variation in sperm quality, endogenous melatonin (MLT), and presumably in the expression of MLT receptors on the sperm throughout the year, which may modify sperm freezability. The aim of this study was to determine whether sperm freezability is associated with (i) endogenous melatonin levels in seminal plasma and (ii) the expression of sperm plasma membrane melatonin receptors (MT1, MT2). To evaluate this, spermatozoa from seven Saanen goat bucks were cryopreserved throughout the year in Mexico using a standard freezing protocol. Seminal plasma MLT concentrations were determined by ELISA and the expression and localization of MT1 and MT2 were detected by immunocytochemistry and confirmed by western blotting. The recovery rate of progressive motility after thawing was higher in spring than autumn and winter; in contrast, the F pattern (CTC assay) was higher in winter than in the other seasons. A proportional increase in the AR pattern (CTC assay) was smaller in winter than in the other seasons and the proportion of sperm showing high plasma membrane fluidity was higher in spring than in summer and autumn. The seminal plasma MLT concentrations showed no significant interseasonal differences. The MT1 receptor was immunolocalised at the apical region of the sperm head, while MT2 was mainly localised in the neck. The relative expression of MLT receptors showed significant differences between summer and winter for all bands, except at 75 kDa of MT2. In conclusion, there was an association between the relative expression of MT1 and MT2 receptors throughout the year and sperm freezability in goat bucks in México. Post-thaw sperm quality is enhanced in semen samples collected during breeding season.
•MLT concentration in goats' seminal plasma does not show interseasonal variation.•Expression of MLT receptors in goat spermatozoa shows variation throughout the year.•Goat buck sperm freezability is associated with the expression of MLT receptors.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>38801791</pmid><doi>10.1016/j.theriogenology.2024.05.034</doi><tpages>9</tpages><orcidid>https://orcid.org/0009-0004-8750-8399</orcidid></addata></record> |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Caprine semen ELISA Immunofluorescence Seasonality Western blotting |
| title | Sperm melatonin receptors, seminal plasma melatonin and semen freezability in goats |
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