Two Enterococcus faecium Isolates Demonstrated Modulating Effects on the Dysbiosis of Mice Gut Microbiota Induced by Antibiotic Treatment
Broad-spectrum antibiotics are frequently used to treat bacteria-induced infections, but the overuse of antibiotics may induce the gut microbiota dysbiosis and disrupt gastrointestinal tract function. Probiotics can be applied to restore disturbed gut microbiota and repair abnormal intestinal metabo...
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| Veröffentlicht in: | International journal of molecular sciences 2024-05, Vol.25 (10), p.5405 |
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| creator | Yao, Xiaohui Nie, Wansen Chen, Xi Zhang, Junjie Wei, Jianchao Qiu, Yafeng Liu, Ke Shao, Donghua Liu, Haixia Ma, Zhiyong Li, Zongjie Li, Beibei |
| description | Broad-spectrum antibiotics are frequently used to treat bacteria-induced infections, but the overuse of antibiotics may induce the gut microbiota dysbiosis and disrupt gastrointestinal tract function. Probiotics can be applied to restore disturbed gut microbiota and repair abnormal intestinal metabolism. In the present study, two strains of
(named DC-K7 and DC-K9) were isolated and characterized from the fecal samples of infant dogs. The genomic features of
DC-K7 and DC-K9 were analyzed, the carbohydrate-active enzyme (CAZyme)-encoding genes were predicted, and their abilities to produce short-chain fatty acids (SCFAs) were investigated. The bacteriocin-encoding genes in the genome sequences of
DC-K7 and DC-K9 were analyzed, and the gene cluster of Enterolysin-A, which encoded a 401-amino-acid peptide, was predicted. Moreover, the modulating effects of
DC-K7 and DC-K9 on the gut microbiota dysbiosis induced by antibiotics were analyzed. The current results demonstrated that oral administrations of
DC-K7 and DC-K9 could enhance the relative abundances of beneficial microbes and decrease the relative abundances of harmful microbes. Therefore, the isolated
DC-K7 and DC-K9 were proven to be able to alter the gut microbiota dysbiosis induced by antibiotic treatment. |
| doi_str_mv | 10.3390/ijms25105405 |
| format | Article |
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(named DC-K7 and DC-K9) were isolated and characterized from the fecal samples of infant dogs. The genomic features of
DC-K7 and DC-K9 were analyzed, the carbohydrate-active enzyme (CAZyme)-encoding genes were predicted, and their abilities to produce short-chain fatty acids (SCFAs) were investigated. The bacteriocin-encoding genes in the genome sequences of
DC-K7 and DC-K9 were analyzed, and the gene cluster of Enterolysin-A, which encoded a 401-amino-acid peptide, was predicted. Moreover, the modulating effects of
DC-K7 and DC-K9 on the gut microbiota dysbiosis induced by antibiotics were analyzed. The current results demonstrated that oral administrations of
DC-K7 and DC-K9 could enhance the relative abundances of beneficial microbes and decrease the relative abundances of harmful microbes. Therefore, the isolated
DC-K7 and DC-K9 were proven to be able to alter the gut microbiota dysbiosis induced by antibiotic treatment.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms25105405</identifier><identifier>PMID: 38791443</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Bacteria ; Bacteriocins - pharmacology ; Carbohydrates ; Diarrhea ; Dogs ; Dysbiosis - microbiology ; Enterococcus faecium ; Fatty Acids, Volatile - metabolism ; Feces - microbiology ; Gastrointestinal Microbiome - drug effects ; Genomes ; Infections ; Metabolism ; Mice ; Microbiota ; Oral administration ; Peptides ; Probiotics ; Probiotics - pharmacology ; Transfer RNA</subject><ispartof>International journal of molecular sciences, 2024-05, Vol.25 (10), p.5405</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c314t-3da304cb85146bd6b7f1bbead091c84e4ffcba0eec3a3e028725cd810fa78b473</cites><orcidid>0000-0002-3246-6273 ; 0000-0003-2967-8686 ; 0000-0002-4134-4024 ; 0000-0003-4211-0817</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38791443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yao, Xiaohui</creatorcontrib><creatorcontrib>Nie, Wansen</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><creatorcontrib>Zhang, Junjie</creatorcontrib><creatorcontrib>Wei, Jianchao</creatorcontrib><creatorcontrib>Qiu, Yafeng</creatorcontrib><creatorcontrib>Liu, Ke</creatorcontrib><creatorcontrib>Shao, Donghua</creatorcontrib><creatorcontrib>Liu, Haixia</creatorcontrib><creatorcontrib>Ma, Zhiyong</creatorcontrib><creatorcontrib>Li, Zongjie</creatorcontrib><creatorcontrib>Li, Beibei</creatorcontrib><title>Two Enterococcus faecium Isolates Demonstrated Modulating Effects on the Dysbiosis of Mice Gut Microbiota Induced by Antibiotic Treatment</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Broad-spectrum antibiotics are frequently used to treat bacteria-induced infections, but the overuse of antibiotics may induce the gut microbiota dysbiosis and disrupt gastrointestinal tract function. Probiotics can be applied to restore disturbed gut microbiota and repair abnormal intestinal metabolism. In the present study, two strains of
(named DC-K7 and DC-K9) were isolated and characterized from the fecal samples of infant dogs. The genomic features of
DC-K7 and DC-K9 were analyzed, the carbohydrate-active enzyme (CAZyme)-encoding genes were predicted, and their abilities to produce short-chain fatty acids (SCFAs) were investigated. The bacteriocin-encoding genes in the genome sequences of
DC-K7 and DC-K9 were analyzed, and the gene cluster of Enterolysin-A, which encoded a 401-amino-acid peptide, was predicted. Moreover, the modulating effects of
DC-K7 and DC-K9 on the gut microbiota dysbiosis induced by antibiotics were analyzed. The current results demonstrated that oral administrations of
DC-K7 and DC-K9 could enhance the relative abundances of beneficial microbes and decrease the relative abundances of harmful microbes. Therefore, the isolated
DC-K7 and DC-K9 were proven to be able to alter the gut microbiota dysbiosis induced by antibiotic treatment.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacteriocins - pharmacology</subject><subject>Carbohydrates</subject><subject>Diarrhea</subject><subject>Dogs</subject><subject>Dysbiosis - microbiology</subject><subject>Enterococcus faecium</subject><subject>Fatty Acids, Volatile - metabolism</subject><subject>Feces - microbiology</subject><subject>Gastrointestinal Microbiome - drug effects</subject><subject>Genomes</subject><subject>Infections</subject><subject>Metabolism</subject><subject>Mice</subject><subject>Microbiota</subject><subject>Oral administration</subject><subject>Peptides</subject><subject>Probiotics</subject><subject>Probiotics - pharmacology</subject><subject>Transfer RNA</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkUtrHDEQhEVIiB_JLecgyCUHb9ya1uzMHI29dhZsfNmcB0nTSrTsSI4emP0J-dfWYieYnLq6-Ci6KcY-CfiGOMC5286paQW0Eto37FjIplkALLu3r_QRO0lpC9Bg0w7v2RH23SCkxGP2Z_MY-MpnisEEY0riVpFxZebrFHYqU-JXNAefcqzLxO_CVKrt_E--spZMTjx4nn8Rv9on7UJy1bD8zhniNyUfRAzVz4qv_VRMjdB7fuGzO5jO8E0klWfy-QN7Z9Uu0ceXecp-XK82l98Xt_c368uL24VBIfMCJ4Ugje5bIZd6WurOCq1JTTAI00uS1hqtgMigQoKm75rWTL0Aq7peyw5P2dfn3IcYfhdKeZxdMrTbKU-hpBFhCdhjA31Fv_yHbkOJvl5XqXaQKBAP1NkzVT9NKZIdH6KbVdyPAsZDRePriir--SW06Jmmf_DfTvAJejCOvw</recordid><startdate>20240515</startdate><enddate>20240515</enddate><creator>Yao, Xiaohui</creator><creator>Nie, Wansen</creator><creator>Chen, Xi</creator><creator>Zhang, Junjie</creator><creator>Wei, Jianchao</creator><creator>Qiu, Yafeng</creator><creator>Liu, Ke</creator><creator>Shao, Donghua</creator><creator>Liu, Haixia</creator><creator>Ma, Zhiyong</creator><creator>Li, Zongjie</creator><creator>Li, Beibei</creator><general>MDPI AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3246-6273</orcidid><orcidid>https://orcid.org/0000-0003-2967-8686</orcidid><orcidid>https://orcid.org/0000-0002-4134-4024</orcidid><orcidid>https://orcid.org/0000-0003-4211-0817</orcidid></search><sort><creationdate>20240515</creationdate><title>Two Enterococcus faecium Isolates Demonstrated Modulating Effects on the Dysbiosis of Mice Gut Microbiota Induced by Antibiotic Treatment</title><author>Yao, Xiaohui ; 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Probiotics can be applied to restore disturbed gut microbiota and repair abnormal intestinal metabolism. In the present study, two strains of
(named DC-K7 and DC-K9) were isolated and characterized from the fecal samples of infant dogs. The genomic features of
DC-K7 and DC-K9 were analyzed, the carbohydrate-active enzyme (CAZyme)-encoding genes were predicted, and their abilities to produce short-chain fatty acids (SCFAs) were investigated. The bacteriocin-encoding genes in the genome sequences of
DC-K7 and DC-K9 were analyzed, and the gene cluster of Enterolysin-A, which encoded a 401-amino-acid peptide, was predicted. Moreover, the modulating effects of
DC-K7 and DC-K9 on the gut microbiota dysbiosis induced by antibiotics were analyzed. The current results demonstrated that oral administrations of
DC-K7 and DC-K9 could enhance the relative abundances of beneficial microbes and decrease the relative abundances of harmful microbes. Therefore, the isolated
DC-K7 and DC-K9 were proven to be able to alter the gut microbiota dysbiosis induced by antibiotic treatment.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38791443</pmid><doi>10.3390/ijms25105405</doi><orcidid>https://orcid.org/0000-0002-3246-6273</orcidid><orcidid>https://orcid.org/0000-0003-2967-8686</orcidid><orcidid>https://orcid.org/0000-0002-4134-4024</orcidid><orcidid>https://orcid.org/0000-0003-4211-0817</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Anti-Bacterial Agents - pharmacology Antibiotics Bacteria Bacteriocins - pharmacology Carbohydrates Diarrhea Dogs Dysbiosis - microbiology Enterococcus faecium Fatty Acids, Volatile - metabolism Feces - microbiology Gastrointestinal Microbiome - drug effects Genomes Infections Metabolism Mice Microbiota Oral administration Peptides Probiotics Probiotics - pharmacology Transfer RNA |
| title | Two Enterococcus faecium Isolates Demonstrated Modulating Effects on the Dysbiosis of Mice Gut Microbiota Induced by Antibiotic Treatment |
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