Brucine Sulfate, a Novel Bacteriostatic Agent in 3D Printed Bone Scaffold Systems
Bacterial infection is a common complication in bone defect surgery, in which infection by clinically resistant bacteria has been a challenge for the medical community. Given this emerging problem, the discovery of novel natural-type inhibitors of drug-resistant bacteria has become imperative. Bruci...
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| Veröffentlicht in: | Polymers 2024-05, Vol.16 (10), p.1428 |
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| creator | Li, Jinying Hu, Shi Feng, Pei Xia, Yang Pei, Zihan Tian, Jiaxuan Jiang, Kun Liu, Liang Cai, Xiong Wu, Ping |
| description | Bacterial infection is a common complication in bone defect surgery, in which infection by clinically resistant bacteria has been a challenge for the medical community. Given this emerging problem, the discovery of novel natural-type inhibitors of drug-resistant bacteria has become imperative. Brucine, present in the traditional Chinese herb
, is reported to exert analgesic and anti-inflammatory effects. Brucine's clinical application was limited because of its water solubility. We extracted high-purity BS by employing reflux extraction and crystallization, greatly improved its solubility, and evaluated its antimicrobial activity against
and
. Importantly, we found that BS inhibited the drug-resistant strains significantly better than standard strains and achieved sterilization by disrupting the bacterial cell wall. Considering the safety concerns associated with the narrow therapeutic window of BS, a 3D BS-PLLA/PGA bone scaffold system was constructed with SLS technology and tested for its performance, bacteriostatic behaviors, and biocompatibility. The results have shown that the drug-loaded bone scaffolds had not only long-term, slow-controlled release with good cytocompatibility but also demonstrated significant antimicrobial activity in antimicrobial testing. The above results indicated that BS may be a potential drug candidate for the treatment of antibiotic-resistant bacterial infections and that scaffolds with enhanced antibacterial activity and mechanical properties may have potential applications in bone tissue engineering. |
| doi_str_mv | 10.3390/polym16101428 |
| format | Article |
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, is reported to exert analgesic and anti-inflammatory effects. Brucine's clinical application was limited because of its water solubility. We extracted high-purity BS by employing reflux extraction and crystallization, greatly improved its solubility, and evaluated its antimicrobial activity against
and
. Importantly, we found that BS inhibited the drug-resistant strains significantly better than standard strains and achieved sterilization by disrupting the bacterial cell wall. Considering the safety concerns associated with the narrow therapeutic window of BS, a 3D BS-PLLA/PGA bone scaffold system was constructed with SLS technology and tested for its performance, bacteriostatic behaviors, and biocompatibility. The results have shown that the drug-loaded bone scaffolds had not only long-term, slow-controlled release with good cytocompatibility but also demonstrated significant antimicrobial activity in antimicrobial testing. The above results indicated that BS may be a potential drug candidate for the treatment of antibiotic-resistant bacterial infections and that scaffolds with enhanced antibacterial activity and mechanical properties may have potential applications in bone tissue engineering.</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym16101428</identifier><identifier>PMID: 38794621</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Alkaloids ; Analysis ; Antibiotics ; Antiinfectives and antibacterials ; Antimicrobial agents ; Bacteria ; Bacterial infections ; Biocompatibility ; Controlled release ; Crystal defects ; Crystallization ; Drug resistance ; E coli ; Extraction (Chemistry) ; Hydrocarbons ; Identification and classification ; Lasers ; Mechanical properties ; Methods ; Molecular weight ; Pharmaceuticals ; Properties ; Scaffolds ; Seeds ; Solubility ; Staphylococcus infections ; Sterilization ; Strychnine ; Sulfates ; Tissue engineering</subject><ispartof>Polymers, 2024-05, Vol.16 (10), p.1428</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c344t-ef612f96ae0de7f1ced81d36339d0728b6ac7bcab63dad1d6a92a3707865e3df3</cites><orcidid>0000-0003-0400-2110</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38794621$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Jinying</creatorcontrib><creatorcontrib>Hu, Shi</creatorcontrib><creatorcontrib>Feng, Pei</creatorcontrib><creatorcontrib>Xia, Yang</creatorcontrib><creatorcontrib>Pei, Zihan</creatorcontrib><creatorcontrib>Tian, Jiaxuan</creatorcontrib><creatorcontrib>Jiang, Kun</creatorcontrib><creatorcontrib>Liu, Liang</creatorcontrib><creatorcontrib>Cai, Xiong</creatorcontrib><creatorcontrib>Wu, Ping</creatorcontrib><title>Brucine Sulfate, a Novel Bacteriostatic Agent in 3D Printed Bone Scaffold Systems</title><title>Polymers</title><addtitle>Polymers (Basel)</addtitle><description>Bacterial infection is a common complication in bone defect surgery, in which infection by clinically resistant bacteria has been a challenge for the medical community. Given this emerging problem, the discovery of novel natural-type inhibitors of drug-resistant bacteria has become imperative. Brucine, present in the traditional Chinese herb
, is reported to exert analgesic and anti-inflammatory effects. Brucine's clinical application was limited because of its water solubility. We extracted high-purity BS by employing reflux extraction and crystallization, greatly improved its solubility, and evaluated its antimicrobial activity against
and
. Importantly, we found that BS inhibited the drug-resistant strains significantly better than standard strains and achieved sterilization by disrupting the bacterial cell wall. Considering the safety concerns associated with the narrow therapeutic window of BS, a 3D BS-PLLA/PGA bone scaffold system was constructed with SLS technology and tested for its performance, bacteriostatic behaviors, and biocompatibility. The results have shown that the drug-loaded bone scaffolds had not only long-term, slow-controlled release with good cytocompatibility but also demonstrated significant antimicrobial activity in antimicrobial testing. The above results indicated that BS may be a potential drug candidate for the treatment of antibiotic-resistant bacterial infections and that scaffolds with enhanced antibacterial activity and mechanical properties may have potential applications in bone tissue engineering.</description><subject>Alkaloids</subject><subject>Analysis</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Biocompatibility</subject><subject>Controlled release</subject><subject>Crystal defects</subject><subject>Crystallization</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Extraction (Chemistry)</subject><subject>Hydrocarbons</subject><subject>Identification and classification</subject><subject>Lasers</subject><subject>Mechanical properties</subject><subject>Methods</subject><subject>Molecular weight</subject><subject>Pharmaceuticals</subject><subject>Properties</subject><subject>Scaffolds</subject><subject>Seeds</subject><subject>Solubility</subject><subject>Staphylococcus infections</subject><subject>Sterilization</subject><subject>Strychnine</subject><subject>Sulfates</subject><subject>Tissue engineering</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpd0TtPwzAQB3ALgSgqHVmRJRYGAnac2MnYlqdU8VBhjhz7XKVK4mI7SP32pGp5ejkPP9-d9UfohJJLxnJytbL1uqGcEprE2R46iolgUcI42f91H6CR90vSnyTlnIpDNGCZyBMe0yP0MnGdqlrA8642MsAFlvjRfkCNJ1IFcJX1QYZK4fEC2oCrFrNr_OyqNoDGE7t5qKQxttZ4vvYBGn-MDoysPYx2dYjebm9ep_fR7OnuYTqeRYolSYjAcBqbnEsgGoShCnRGNeP9vzQRcVZyqUSpZMmZlppqLvNYMkFExlNg2rAhOt_2XTn73oEPRVN5BXUtW7CdLxjhhAnBWdzTs390aTvX9tv1Ks15KvKY_KiFrKGoWmODk2rTtBiLPE1SmpK0V9FWKWe9d2CKlasa6dYFJcUmlOJPKL0_3c3uygb0t_6KgH0CpnOFpA</recordid><startdate>20240517</startdate><enddate>20240517</enddate><creator>Li, Jinying</creator><creator>Hu, Shi</creator><creator>Feng, Pei</creator><creator>Xia, Yang</creator><creator>Pei, Zihan</creator><creator>Tian, Jiaxuan</creator><creator>Jiang, Kun</creator><creator>Liu, Liang</creator><creator>Cai, Xiong</creator><creator>Wu, Ping</creator><general>MDPI AG</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0400-2110</orcidid></search><sort><creationdate>20240517</creationdate><title>Brucine Sulfate, a Novel Bacteriostatic Agent in 3D Printed Bone Scaffold Systems</title><author>Li, Jinying ; Hu, Shi ; Feng, Pei ; Xia, Yang ; Pei, Zihan ; Tian, Jiaxuan ; Jiang, Kun ; Liu, Liang ; Cai, Xiong ; Wu, Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-ef612f96ae0de7f1ced81d36339d0728b6ac7bcab63dad1d6a92a3707865e3df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Alkaloids</topic><topic>Analysis</topic><topic>Antibiotics</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Bacterial infections</topic><topic>Biocompatibility</topic><topic>Controlled release</topic><topic>Crystal defects</topic><topic>Crystallization</topic><topic>Drug resistance</topic><topic>E coli</topic><topic>Extraction (Chemistry)</topic><topic>Hydrocarbons</topic><topic>Identification and classification</topic><topic>Lasers</topic><topic>Mechanical properties</topic><topic>Methods</topic><topic>Molecular weight</topic><topic>Pharmaceuticals</topic><topic>Properties</topic><topic>Scaffolds</topic><topic>Seeds</topic><topic>Solubility</topic><topic>Staphylococcus infections</topic><topic>Sterilization</topic><topic>Strychnine</topic><topic>Sulfates</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jinying</creatorcontrib><creatorcontrib>Hu, Shi</creatorcontrib><creatorcontrib>Feng, Pei</creatorcontrib><creatorcontrib>Xia, Yang</creatorcontrib><creatorcontrib>Pei, Zihan</creatorcontrib><creatorcontrib>Tian, Jiaxuan</creatorcontrib><creatorcontrib>Jiang, Kun</creatorcontrib><creatorcontrib>Liu, Liang</creatorcontrib><creatorcontrib>Cai, Xiong</creatorcontrib><creatorcontrib>Wu, Ping</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jinying</au><au>Hu, Shi</au><au>Feng, Pei</au><au>Xia, Yang</au><au>Pei, Zihan</au><au>Tian, Jiaxuan</au><au>Jiang, Kun</au><au>Liu, Liang</au><au>Cai, Xiong</au><au>Wu, Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Brucine Sulfate, a Novel Bacteriostatic Agent in 3D Printed Bone Scaffold Systems</atitle><jtitle>Polymers</jtitle><addtitle>Polymers (Basel)</addtitle><date>2024-05-17</date><risdate>2024</risdate><volume>16</volume><issue>10</issue><spage>1428</spage><pages>1428-</pages><issn>2073-4360</issn><eissn>2073-4360</eissn><abstract>Bacterial infection is a common complication in bone defect surgery, in which infection by clinically resistant bacteria has been a challenge for the medical community. Given this emerging problem, the discovery of novel natural-type inhibitors of drug-resistant bacteria has become imperative. Brucine, present in the traditional Chinese herb
, is reported to exert analgesic and anti-inflammatory effects. Brucine's clinical application was limited because of its water solubility. We extracted high-purity BS by employing reflux extraction and crystallization, greatly improved its solubility, and evaluated its antimicrobial activity against
and
. Importantly, we found that BS inhibited the drug-resistant strains significantly better than standard strains and achieved sterilization by disrupting the bacterial cell wall. Considering the safety concerns associated with the narrow therapeutic window of BS, a 3D BS-PLLA/PGA bone scaffold system was constructed with SLS technology and tested for its performance, bacteriostatic behaviors, and biocompatibility. The results have shown that the drug-loaded bone scaffolds had not only long-term, slow-controlled release with good cytocompatibility but also demonstrated significant antimicrobial activity in antimicrobial testing. The above results indicated that BS may be a potential drug candidate for the treatment of antibiotic-resistant bacterial infections and that scaffolds with enhanced antibacterial activity and mechanical properties may have potential applications in bone tissue engineering.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38794621</pmid><doi>10.3390/polym16101428</doi><orcidid>https://orcid.org/0000-0003-0400-2110</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Alkaloids Analysis Antibiotics Antiinfectives and antibacterials Antimicrobial agents Bacteria Bacterial infections Biocompatibility Controlled release Crystal defects Crystallization Drug resistance E coli Extraction (Chemistry) Hydrocarbons Identification and classification Lasers Mechanical properties Methods Molecular weight Pharmaceuticals Properties Scaffolds Seeds Solubility Staphylococcus infections Sterilization Strychnine Sulfates Tissue engineering |
| title | Brucine Sulfate, a Novel Bacteriostatic Agent in 3D Printed Bone Scaffold Systems |
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