Elevated CO2 induce alterations in the hormonal regulation of stomata in drought stressed tomato seedlings

The atmospheric CO2 level is rising, and the consequent climate change is causing an increase in drought events. Furthermore, the CO2 level is known to induce changes in the physiological responses to stress in plants. Exogenous melatonin is suggested to play roles in the response of plants to abiotic stresses, including drought. We investigated physiological drought stress responses at ambient and elevated CO2 levels (aCO2 and eCO2) of melatonin-treated and untreated tomato plants, aiming to link effects of water use efficiency of photosynthesis at (WUELeaf) and stomatal conductance (gs) with the hormonal regulation of stomata. Tomatoes grown at eCO2 had reduced water use of both irrigated and drought stressed plants during the progression of drought at the whole plant level. This was also reflected in a CO2-affected increase in WUELeaf at eCO2 across irrigated and drought-stressed plants. These CO2-induced effects were mediated through stomatal closing and reductions in stomatal pore area rather than stomatal density or size. Abscisic acid (ABA) and its conjugated form, ABA glucose ester (ABA-GE), increased at drought stress in aCO2, while only ABA-GE increased at eCO2. Contrary, salicylic acid (SA) increased to a greater magnitude at drought stress in eCO2 than aCO2. Melatonin treatment showed no effects on the stomatal regulation. Our findings imply that eCO2 changes in the balance of hormonal effectors in stomatal regulation during drought, shifting from it ABA to SA regulation, suggesting to consider stomatal reactions at eCO2 in a perspective of a hormonal interplay rather than only ABA. •ECO2 reduced water use of both irrigated and drought stressed plants.•ABA increased in tomato in response to drought at aCO2, but not at eCO2.•Salicylic acid increased in drought stress to a larger extend at eCO2 than at aCO2.•Exogenous melatonin had no effect on stomatal regulation or associated hormones.