Characterization of Actin-based Genotypes and Mycoplasma Endosymbionts of Trichomonas vaginalis Isolated in Sapporo, Japan
Purpose Trichomonas vaginali s is a causative agent of common non-viral sexually transmitted infections worldwide. However, the biological features, such as genotypes and endosymbionts, of T. vaginalis isolated in Japan remain unclear. The aim of this study was to characterize the actin-based genoty...
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| Veröffentlicht in: | Acta parasitologica 2024-06, Vol.69 (2), p.1324-1328 |
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| creator | Yamazaki, Tomohiro Sawai, Kyohei Takahashi, Yuji Matsuo, Junji |
| description | Purpose
Trichomonas vaginali
s is a causative agent of common non-viral sexually transmitted infections worldwide. However, the biological features, such as genotypes and endosymbionts, of
T. vaginalis
isolated in Japan remain unclear. The aim of this study was to characterize the actin-based genotypes and the endosymbionts of
T. vaginalis
isolated in Sapporo, Japan.
Methods
Three
T. vaginalis
clinical strains were isolated in Sapporo, Japan between 2019 and 2022. Actin-based genotyping was conducted by sequencing and phylogenetic analyses. The endosymbionts, such as
Mycoplasma
sp. and Trichomonasvirus, were detected using PCR and RT-PCR, respectively. Furthermore, the detected
Mycoplasma
spp. were identified using 16S rRNA gene sequencing.
Results
Of the three
T. vaginalis
strains, two belonged to genotype E, whereas one was genotype G as determined by actin-based genotyping. Two of the
T. vaginalis
strains harbored
Mycoplasma
spp. Using nearly full-length 16S rRNA gene sequencing, both were identified as
Candidatus
Mycoplasma girerdii. In contrast, the Trichomonasvirus was not found in the
T. vaginalis
strains.
Conclusion
To our knowledge, this is the first report on the characterization of actin-based genotypes and the presence of endosymbiotic
Ca.
M. girerdii in
T. vaginalis
strains in Japan. Thus, this study will provide an important impetus for future research. |
| doi_str_mv | 10.1007/s11686-024-00853-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3058636350</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3069194603</sourcerecordid><originalsourceid>FETCH-LOGICAL-c326t-2cd21007b057739e1689979edc7ea054286e708895ff2b83ff51e7b668abc6053</originalsourceid><addsrcrecordid>eNp9kUtvFDEQhC0EIg_4AxyQJS4cMPixfh2jVUiCgnIgnK0ejydxNGMP9izS5tfHmw0g5cDJLfmr6i4VQu8Y_cwo1V8qY8ooQvmKUGqkIOYFOmTGKsKMZC_bzAUl3HB2gI5qvaN0pYwxr9GBMFpLy9Qhul_fQgG_hBLvYYk54TzgE7_ERDqoocdnIeVlO4eKIfX4-9bneYQ6AT5Nfa7bqWuape5U1yX62zzlBBX_hpuYYIwVX9Q8wtKMYsI_YJ5zyZ_wN5ghvUGvBhhrePv0HqOfX0-v1-fk8ursYn1ySbzgaiHc93wXt6NSa2FDy2yttqH3OgCVK25U0NQYK4eBd0YMg2RBd0oZ6LyiUhyjj3vfueRfm1AXN8XqwzhCCnlTnaDSKKGEpA398Ay9y5vSguwoZZldKSoaxfeUL7nWEgY3lzhB2TpG3e5Ut2_GtWbcYzPONNH7J-tNN4X-r-RPFQ0Qe6C2r3QTyr_d_7F9AGFhmZw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3069194603</pqid></control><display><type>article</type><title>Characterization of Actin-based Genotypes and Mycoplasma Endosymbionts of Trichomonas vaginalis Isolated in Sapporo, Japan</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Yamazaki, Tomohiro ; Sawai, Kyohei ; Takahashi, Yuji ; Matsuo, Junji</creator><creatorcontrib>Yamazaki, Tomohiro ; Sawai, Kyohei ; Takahashi, Yuji ; Matsuo, Junji</creatorcontrib><description>Purpose
Trichomonas vaginali
s is a causative agent of common non-viral sexually transmitted infections worldwide. However, the biological features, such as genotypes and endosymbionts, of
T. vaginalis
isolated in Japan remain unclear. The aim of this study was to characterize the actin-based genotypes and the endosymbionts of
T. vaginalis
isolated in Sapporo, Japan.
Methods
Three
T. vaginalis
clinical strains were isolated in Sapporo, Japan between 2019 and 2022. Actin-based genotyping was conducted by sequencing and phylogenetic analyses. The endosymbionts, such as
Mycoplasma
sp. and Trichomonasvirus, were detected using PCR and RT-PCR, respectively. Furthermore, the detected
Mycoplasma
spp. were identified using 16S rRNA gene sequencing.
Results
Of the three
T. vaginalis
strains, two belonged to genotype E, whereas one was genotype G as determined by actin-based genotyping. Two of the
T. vaginalis
strains harbored
Mycoplasma
spp. Using nearly full-length 16S rRNA gene sequencing, both were identified as
Candidatus
Mycoplasma girerdii. In contrast, the Trichomonasvirus was not found in the
T. vaginalis
strains.
Conclusion
To our knowledge, this is the first report on the characterization of actin-based genotypes and the presence of endosymbiotic
Ca.
M. girerdii in
T. vaginalis
strains in Japan. Thus, this study will provide an important impetus for future research.</description><identifier>ISSN: 1230-2821</identifier><identifier>EISSN: 1896-1851</identifier><identifier>DOI: 10.1007/s11686-024-00853-8</identifier><identifier>PMID: 38775916</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Actin ; Actins - genetics ; Animal Systematics/Taxonomy/Biogeography ; Biomedical and Life Sciences ; Biomedicine ; DNA sequencing ; Ecology ; Endosymbionts ; Female ; Gene sequencing ; Genotype ; Genotypes ; Genotyping ; Humans ; Japan ; Medical Microbiology ; Microbiology ; Mycoplasma ; Mycoplasma - classification ; Mycoplasma - genetics ; Mycoplasma - isolation & purification ; Parasitology ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sexually transmitted diseases ; Short Communication ; STD ; Strains (organisms) ; Symbiosis ; Trichomonas vaginalis ; Trichomonas vaginalis - genetics ; Trichomonas vaginalis - isolation & purification ; Trichomonas Vaginitis - parasitology</subject><ispartof>Acta parasitologica, 2024-06, Vol.69 (2), p.1324-1328</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2024</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.</rights><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2024.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-2cd21007b057739e1689979edc7ea054286e708895ff2b83ff51e7b668abc6053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11686-024-00853-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11686-024-00853-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38775916$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamazaki, Tomohiro</creatorcontrib><creatorcontrib>Sawai, Kyohei</creatorcontrib><creatorcontrib>Takahashi, Yuji</creatorcontrib><creatorcontrib>Matsuo, Junji</creatorcontrib><title>Characterization of Actin-based Genotypes and Mycoplasma Endosymbionts of Trichomonas vaginalis Isolated in Sapporo, Japan</title><title>Acta parasitologica</title><addtitle>Acta Parasit</addtitle><addtitle>Acta Parasitol</addtitle><description>Purpose
Trichomonas vaginali
s is a causative agent of common non-viral sexually transmitted infections worldwide. However, the biological features, such as genotypes and endosymbionts, of
T. vaginalis
isolated in Japan remain unclear. The aim of this study was to characterize the actin-based genotypes and the endosymbionts of
T. vaginalis
isolated in Sapporo, Japan.
Methods
Three
T. vaginalis
clinical strains were isolated in Sapporo, Japan between 2019 and 2022. Actin-based genotyping was conducted by sequencing and phylogenetic analyses. The endosymbionts, such as
Mycoplasma
sp. and Trichomonasvirus, were detected using PCR and RT-PCR, respectively. Furthermore, the detected
Mycoplasma
spp. were identified using 16S rRNA gene sequencing.
Results
Of the three
T. vaginalis
strains, two belonged to genotype E, whereas one was genotype G as determined by actin-based genotyping. Two of the
T. vaginalis
strains harbored
Mycoplasma
spp. Using nearly full-length 16S rRNA gene sequencing, both were identified as
Candidatus
Mycoplasma girerdii. In contrast, the Trichomonasvirus was not found in the
T. vaginalis
strains.
Conclusion
To our knowledge, this is the first report on the characterization of actin-based genotypes and the presence of endosymbiotic
Ca.
M. girerdii in
T. vaginalis
strains in Japan. Thus, this study will provide an important impetus for future research.</description><subject>Actin</subject><subject>Actins - genetics</subject><subject>Animal Systematics/Taxonomy/Biogeography</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>DNA sequencing</subject><subject>Ecology</subject><subject>Endosymbionts</subject><subject>Female</subject><subject>Gene sequencing</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Genotyping</subject><subject>Humans</subject><subject>Japan</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Mycoplasma</subject><subject>Mycoplasma - classification</subject><subject>Mycoplasma - genetics</subject><subject>Mycoplasma - isolation & purification</subject><subject>Parasitology</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Sexually transmitted diseases</subject><subject>Short Communication</subject><subject>STD</subject><subject>Strains (organisms)</subject><subject>Symbiosis</subject><subject>Trichomonas vaginalis</subject><subject>Trichomonas vaginalis - genetics</subject><subject>Trichomonas vaginalis - isolation & purification</subject><subject>Trichomonas Vaginitis - parasitology</subject><issn>1230-2821</issn><issn>1896-1851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtvFDEQhC0EIg_4AxyQJS4cMPixfh2jVUiCgnIgnK0ejydxNGMP9izS5tfHmw0g5cDJLfmr6i4VQu8Y_cwo1V8qY8ooQvmKUGqkIOYFOmTGKsKMZC_bzAUl3HB2gI5qvaN0pYwxr9GBMFpLy9Qhul_fQgG_hBLvYYk54TzgE7_ERDqoocdnIeVlO4eKIfX4-9bneYQ6AT5Nfa7bqWuape5U1yX62zzlBBX_hpuYYIwVX9Q8wtKMYsI_YJ5zyZ_wN5ghvUGvBhhrePv0HqOfX0-v1-fk8ursYn1ySbzgaiHc93wXt6NSa2FDy2yttqH3OgCVK25U0NQYK4eBd0YMg2RBd0oZ6LyiUhyjj3vfueRfm1AXN8XqwzhCCnlTnaDSKKGEpA398Ay9y5vSguwoZZldKSoaxfeUL7nWEgY3lzhB2TpG3e5Ut2_GtWbcYzPONNH7J-tNN4X-r-RPFQ0Qe6C2r3QTyr_d_7F9AGFhmZw</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Yamazaki, Tomohiro</creator><creator>Sawai, Kyohei</creator><creator>Takahashi, Yuji</creator><creator>Matsuo, Junji</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20240601</creationdate><title>Characterization of Actin-based Genotypes and Mycoplasma Endosymbionts of Trichomonas vaginalis Isolated in Sapporo, Japan</title><author>Yamazaki, Tomohiro ; Sawai, Kyohei ; Takahashi, Yuji ; Matsuo, Junji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-2cd21007b057739e1689979edc7ea054286e708895ff2b83ff51e7b668abc6053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Actin</topic><topic>Actins - genetics</topic><topic>Animal Systematics/Taxonomy/Biogeography</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>DNA sequencing</topic><topic>Ecology</topic><topic>Endosymbionts</topic><topic>Female</topic><topic>Gene sequencing</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Genotyping</topic><topic>Humans</topic><topic>Japan</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Mycoplasma</topic><topic>Mycoplasma - classification</topic><topic>Mycoplasma - genetics</topic><topic>Mycoplasma - isolation & purification</topic><topic>Parasitology</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Sexually transmitted diseases</topic><topic>Short Communication</topic><topic>STD</topic><topic>Strains (organisms)</topic><topic>Symbiosis</topic><topic>Trichomonas vaginalis</topic><topic>Trichomonas vaginalis - genetics</topic><topic>Trichomonas vaginalis - isolation & purification</topic><topic>Trichomonas Vaginitis - parasitology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamazaki, Tomohiro</creatorcontrib><creatorcontrib>Sawai, Kyohei</creatorcontrib><creatorcontrib>Takahashi, Yuji</creatorcontrib><creatorcontrib>Matsuo, Junji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta parasitologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamazaki, Tomohiro</au><au>Sawai, Kyohei</au><au>Takahashi, Yuji</au><au>Matsuo, Junji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Actin-based Genotypes and Mycoplasma Endosymbionts of Trichomonas vaginalis Isolated in Sapporo, Japan</atitle><jtitle>Acta parasitologica</jtitle><stitle>Acta Parasit</stitle><addtitle>Acta Parasitol</addtitle><date>2024-06-01</date><risdate>2024</risdate><volume>69</volume><issue>2</issue><spage>1324</spage><epage>1328</epage><pages>1324-1328</pages><issn>1230-2821</issn><eissn>1896-1851</eissn><abstract>Purpose
Trichomonas vaginali
s is a causative agent of common non-viral sexually transmitted infections worldwide. However, the biological features, such as genotypes and endosymbionts, of
T. vaginalis
isolated in Japan remain unclear. The aim of this study was to characterize the actin-based genotypes and the endosymbionts of
T. vaginalis
isolated in Sapporo, Japan.
Methods
Three
T. vaginalis
clinical strains were isolated in Sapporo, Japan between 2019 and 2022. Actin-based genotyping was conducted by sequencing and phylogenetic analyses. The endosymbionts, such as
Mycoplasma
sp. and Trichomonasvirus, were detected using PCR and RT-PCR, respectively. Furthermore, the detected
Mycoplasma
spp. were identified using 16S rRNA gene sequencing.
Results
Of the three
T. vaginalis
strains, two belonged to genotype E, whereas one was genotype G as determined by actin-based genotyping. Two of the
T. vaginalis
strains harbored
Mycoplasma
spp. Using nearly full-length 16S rRNA gene sequencing, both were identified as
Candidatus
Mycoplasma girerdii. In contrast, the Trichomonasvirus was not found in the
T. vaginalis
strains.
Conclusion
To our knowledge, this is the first report on the characterization of actin-based genotypes and the presence of endosymbiotic
Ca.
M. girerdii in
T. vaginalis
strains in Japan. Thus, this study will provide an important impetus for future research.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>38775916</pmid><doi>10.1007/s11686-024-00853-8</doi><tpages>5</tpages></addata></record> |
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| issn | 1230-2821 1896-1851 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Actin Actins - genetics Animal Systematics/Taxonomy/Biogeography Biomedical and Life Sciences Biomedicine DNA sequencing Ecology Endosymbionts Female Gene sequencing Genotype Genotypes Genotyping Humans Japan Medical Microbiology Microbiology Mycoplasma Mycoplasma - classification Mycoplasma - genetics Mycoplasma - isolation & purification Parasitology Phylogeny RNA, Ribosomal, 16S - genetics rRNA 16S Sexually transmitted diseases Short Communication STD Strains (organisms) Symbiosis Trichomonas vaginalis Trichomonas vaginalis - genetics Trichomonas vaginalis - isolation & purification Trichomonas Vaginitis - parasitology |
| title | Characterization of Actin-based Genotypes and Mycoplasma Endosymbionts of Trichomonas vaginalis Isolated in Sapporo, Japan |
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