A case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorder
Background Alcohol use disorder (AUD) is a relapsing disease described as excessive use of alcohol. Evidence of the role of DNA methylation in addiction is accumulating. Ghrelin is an important peptide known as appetite hormone and its role in addictive behavior has been identified. Here we aimed to...
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| Veröffentlicht in: | Molecular biology reports 2024-12, Vol.51 (1), p.663-663, Article 663 |
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| creator | Özkan-Kotiloğlu, Selin Kaya-Akyüzlü, Dilek Güven, Emine Doğan, Özlem Ağtaş-Ertan, Ece Özgür-İlhan, İnci |
| description | Background
Alcohol use disorder (AUD) is a relapsing disease described as excessive use of alcohol. Evidence of the role of DNA methylation in addiction is accumulating. Ghrelin is an important peptide known as appetite hormone and its role in addictive behavior has been identified. Here we aimed to determine the methylation levels of two crucial genes (
GHRL
and
GHSR
) in ghrelin signaling and further investigate the association between methylation ratios and plasma ghrelin levels.
Methods
Individuals diagnosed with (
n
= 71) and without (
n
= 82) AUD were recruited in this study. DNA methylation levels were measured through methylation-sensitive high-resolution melting (MS-HRM). Acylated ghrelin levels were detected by ELISA. The
GHRL
rs696217 polymorphism was analyzed by the standard PCR-RFLP method.
Results
GHRL
was significantly hypermethylated (
P
|
| doi_str_mv | 10.1007/s11033-024-09585-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3057695432</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3057695432</sourcerecordid><originalsourceid>FETCH-LOGICAL-c326t-b28d70ea761aaa66a4600f722e8460a7db3edd01120a1a63aa1dcf7235276abd3</originalsourceid><addsrcrecordid>eNp9kU1rGzEQhkVpaNy0f6CHIuill21GX6v10ZgkDhgC-TiL8Wpsb1hLqbQb8L-PErst5NCTRszzvjPSy9g3Ab8EgD3PQoBSFUhdwdQ0ptIf2EQYqyo9tc1HNgEFotKNEafsc86PAKCFNZ_YqWqsFXqqJ2w74y1m4m0MQ4o9z8Po97wLz5SHboNDFzZ82BLf0bDd9-UeA-_pmfrM45pfLW6XHIMvxd0t31CgXLQc-zZui9lYjH2XY_KUvrCTNfaZvh7PM_ZweXE_X1TLm6vr-WxZtUrWQ7WSjbdAaGuBiHWNugZYWympKRVav1LkPQghAQXWClH4tvSVkbbGlVdn7OfB9ynF32N5hdt1uaW-x0BxzE6BsfXUaCUL-uMd-hjHFMp2b5TRjZavlDxQbYo5J1q7p9TtMO2dAPeagzvk4EoO7i0Hp4vo-9F6XO3I_5X8-fgCqAOQSytsKP2b_R_bF992knA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3057548422</pqid></control><display><type>article</type><title>A case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorder</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Özkan-Kotiloğlu, Selin ; Kaya-Akyüzlü, Dilek ; Güven, Emine ; Doğan, Özlem ; Ağtaş-Ertan, Ece ; Özgür-İlhan, İnci</creator><creatorcontrib>Özkan-Kotiloğlu, Selin ; Kaya-Akyüzlü, Dilek ; Güven, Emine ; Doğan, Özlem ; Ağtaş-Ertan, Ece ; Özgür-İlhan, İnci</creatorcontrib><description>Background
Alcohol use disorder (AUD) is a relapsing disease described as excessive use of alcohol. Evidence of the role of DNA methylation in addiction is accumulating. Ghrelin is an important peptide known as appetite hormone and its role in addictive behavior has been identified. Here we aimed to determine the methylation levels of two crucial genes (
GHRL
and
GHSR
) in ghrelin signaling and further investigate the association between methylation ratios and plasma ghrelin levels.
Methods
Individuals diagnosed with (
n
= 71) and without (
n
= 82) AUD were recruited in this study. DNA methylation levels were measured through methylation-sensitive high-resolution melting (MS-HRM). Acylated ghrelin levels were detected by ELISA. The
GHRL
rs696217 polymorphism was analyzed by the standard PCR-RFLP method.
Results
GHRL
was significantly hypermethylated (
P
< 0.0022) in AUD between 25 and 50% methylation than in control subjects but no significant changes of
GHSR
methylation were observed. Moreover,
GHRL
showed significant positive correlation of methylation ratio between 25 and 50% with age. A significant positive correlation between
GHSR
methylation and ghrelin levels in the AUD group was determined (
P
= 0.037). The level of
GHRL
methylation and the ghrelin levels showed a significant association in the control subjects (
P
= 0.042).
Conclusion
GHSR
and
GHRL
methylation levels did not change significantly between control and AUD groups. However,
GHRL
and
GHSR
methylations seemed to have associations with plasma ghrelin levels in two groups. This is the first study investigating the DNA methylation of
GHRL
and
GHSR
genes in AUD.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-024-09585-4</identifier><identifier>PMID: 38771494</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Addictions ; Addictive behaviors ; Adult ; Alcohol use ; Alcoholism - genetics ; Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Case-Control Studies ; DNA methylation ; DNA Methylation - genetics ; Female ; Ghrelin ; Ghrelin - blood ; Ghrelin - genetics ; Histology ; Humans ; Life Sciences ; Male ; Middle Aged ; Morphology ; Original Article ; Polymorphism, Single Nucleotide - genetics ; Receptors, Ghrelin - genetics ; Restriction fragment length polymorphism</subject><ispartof>Molecular biology reports, 2024-12, Vol.51 (1), p.663-663, Article 663</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-b28d70ea761aaa66a4600f722e8460a7db3edd01120a1a63aa1dcf7235276abd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-024-09585-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-024-09585-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38771494$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Özkan-Kotiloğlu, Selin</creatorcontrib><creatorcontrib>Kaya-Akyüzlü, Dilek</creatorcontrib><creatorcontrib>Güven, Emine</creatorcontrib><creatorcontrib>Doğan, Özlem</creatorcontrib><creatorcontrib>Ağtaş-Ertan, Ece</creatorcontrib><creatorcontrib>Özgür-İlhan, İnci</creatorcontrib><title>A case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorder</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
Alcohol use disorder (AUD) is a relapsing disease described as excessive use of alcohol. Evidence of the role of DNA methylation in addiction is accumulating. Ghrelin is an important peptide known as appetite hormone and its role in addictive behavior has been identified. Here we aimed to determine the methylation levels of two crucial genes (
GHRL
and
GHSR
) in ghrelin signaling and further investigate the association between methylation ratios and plasma ghrelin levels.
Methods
Individuals diagnosed with (
n
= 71) and without (
n
= 82) AUD were recruited in this study. DNA methylation levels were measured through methylation-sensitive high-resolution melting (MS-HRM). Acylated ghrelin levels were detected by ELISA. The
GHRL
rs696217 polymorphism was analyzed by the standard PCR-RFLP method.
Results
GHRL
was significantly hypermethylated (
P
< 0.0022) in AUD between 25 and 50% methylation than in control subjects but no significant changes of
GHSR
methylation were observed. Moreover,
GHRL
showed significant positive correlation of methylation ratio between 25 and 50% with age. A significant positive correlation between
GHSR
methylation and ghrelin levels in the AUD group was determined (
P
= 0.037). The level of
GHRL
methylation and the ghrelin levels showed a significant association in the control subjects (
P
= 0.042).
Conclusion
GHSR
and
GHRL
methylation levels did not change significantly between control and AUD groups. However,
GHRL
and
GHSR
methylations seemed to have associations with plasma ghrelin levels in two groups. This is the first study investigating the DNA methylation of
GHRL
and
GHSR
genes in AUD.</description><subject>Addictions</subject><subject>Addictive behaviors</subject><subject>Adult</subject><subject>Alcohol use</subject><subject>Alcoholism - genetics</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Case-Control Studies</subject><subject>DNA methylation</subject><subject>DNA Methylation - genetics</subject><subject>Female</subject><subject>Ghrelin</subject><subject>Ghrelin - blood</subject><subject>Ghrelin - genetics</subject><subject>Histology</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Polymorphism, Single Nucleotide - genetics</subject><subject>Receptors, Ghrelin - genetics</subject><subject>Restriction fragment length polymorphism</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1rGzEQhkVpaNy0f6CHIuill21GX6v10ZgkDhgC-TiL8Wpsb1hLqbQb8L-PErst5NCTRszzvjPSy9g3Ab8EgD3PQoBSFUhdwdQ0ptIf2EQYqyo9tc1HNgEFotKNEafsc86PAKCFNZ_YqWqsFXqqJ2w74y1m4m0MQ4o9z8Po97wLz5SHboNDFzZ82BLf0bDd9-UeA-_pmfrM45pfLW6XHIMvxd0t31CgXLQc-zZui9lYjH2XY_KUvrCTNfaZvh7PM_ZweXE_X1TLm6vr-WxZtUrWQ7WSjbdAaGuBiHWNugZYWympKRVav1LkPQghAQXWClH4tvSVkbbGlVdn7OfB9ynF32N5hdt1uaW-x0BxzE6BsfXUaCUL-uMd-hjHFMp2b5TRjZavlDxQbYo5J1q7p9TtMO2dAPeagzvk4EoO7i0Hp4vo-9F6XO3I_5X8-fgCqAOQSytsKP2b_R_bF992knA</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Özkan-Kotiloğlu, Selin</creator><creator>Kaya-Akyüzlü, Dilek</creator><creator>Güven, Emine</creator><creator>Doğan, Özlem</creator><creator>Ağtaş-Ertan, Ece</creator><creator>Özgür-İlhan, İnci</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20241201</creationdate><title>A case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorder</title><author>Özkan-Kotiloğlu, Selin ; Kaya-Akyüzlü, Dilek ; Güven, Emine ; Doğan, Özlem ; Ağtaş-Ertan, Ece ; Özgür-İlhan, İnci</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-b28d70ea761aaa66a4600f722e8460a7db3edd01120a1a63aa1dcf7235276abd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Addictions</topic><topic>Addictive behaviors</topic><topic>Adult</topic><topic>Alcohol use</topic><topic>Alcoholism - genetics</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Case-Control Studies</topic><topic>DNA methylation</topic><topic>DNA Methylation - genetics</topic><topic>Female</topic><topic>Ghrelin</topic><topic>Ghrelin - blood</topic><topic>Ghrelin - genetics</topic><topic>Histology</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Polymorphism, Single Nucleotide - genetics</topic><topic>Receptors, Ghrelin - genetics</topic><topic>Restriction fragment length polymorphism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Özkan-Kotiloğlu, Selin</creatorcontrib><creatorcontrib>Kaya-Akyüzlü, Dilek</creatorcontrib><creatorcontrib>Güven, Emine</creatorcontrib><creatorcontrib>Doğan, Özlem</creatorcontrib><creatorcontrib>Ağtaş-Ertan, Ece</creatorcontrib><creatorcontrib>Özgür-İlhan, İnci</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Özkan-Kotiloğlu, Selin</au><au>Kaya-Akyüzlü, Dilek</au><au>Güven, Emine</au><au>Doğan, Özlem</au><au>Ağtaş-Ertan, Ece</au><au>Özgür-İlhan, İnci</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorder</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>51</volume><issue>1</issue><spage>663</spage><epage>663</epage><pages>663-663</pages><artnum>663</artnum><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
Alcohol use disorder (AUD) is a relapsing disease described as excessive use of alcohol. Evidence of the role of DNA methylation in addiction is accumulating. Ghrelin is an important peptide known as appetite hormone and its role in addictive behavior has been identified. Here we aimed to determine the methylation levels of two crucial genes (
GHRL
and
GHSR
) in ghrelin signaling and further investigate the association between methylation ratios and plasma ghrelin levels.
Methods
Individuals diagnosed with (
n
= 71) and without (
n
= 82) AUD were recruited in this study. DNA methylation levels were measured through methylation-sensitive high-resolution melting (MS-HRM). Acylated ghrelin levels were detected by ELISA. The
GHRL
rs696217 polymorphism was analyzed by the standard PCR-RFLP method.
Results
GHRL
was significantly hypermethylated (
P
< 0.0022) in AUD between 25 and 50% methylation than in control subjects but no significant changes of
GHSR
methylation were observed. Moreover,
GHRL
showed significant positive correlation of methylation ratio between 25 and 50% with age. A significant positive correlation between
GHSR
methylation and ghrelin levels in the AUD group was determined (
P
= 0.037). The level of
GHRL
methylation and the ghrelin levels showed a significant association in the control subjects (
P
= 0.042).
Conclusion
GHSR
and
GHRL
methylation levels did not change significantly between control and AUD groups. However,
GHRL
and
GHSR
methylations seemed to have associations with plasma ghrelin levels in two groups. This is the first study investigating the DNA methylation of
GHRL
and
GHSR
genes in AUD.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>38771494</pmid><doi>10.1007/s11033-024-09585-4</doi><tpages>1</tpages></addata></record> |
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| ispartof | Molecular biology reports, 2024-12, Vol.51 (1), p.663-663, Article 663 |
| issn | 0301-4851 1573-4978 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Addictions Addictive behaviors Adult Alcohol use Alcoholism - genetics Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Case-Control Studies DNA methylation DNA Methylation - genetics Female Ghrelin Ghrelin - blood Ghrelin - genetics Histology Humans Life Sciences Male Middle Aged Morphology Original Article Polymorphism, Single Nucleotide - genetics Receptors, Ghrelin - genetics Restriction fragment length polymorphism |
| title | A case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorder |
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