Comparative characteristics of the stem cells’ number in the stromal vascular fraction of infrapatellar fat pad and subcutaneous fat tissue
The use of infrapatellar fat pad adipose stem cells (IPFP-ASCs) shows an age-independent proliferation and differentiation potential. In addition, the pronounced chondrogenic potential of IPFP-ASCs makes them promising candidates for research for use in other methods of regenerative therapy. The pur...
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| Veröffentlicht in: | Journal of ISAKOS 2024-08, Vol.9 (4), p.615-619 |
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| creator | Maslennikov, Serhii Avramenko, Yuliia Tumanskiy, Valeriy Golovakha, Maksym |
| description | The use of infrapatellar fat pad adipose stem cells (IPFP-ASCs) shows an age-independent proliferation and differentiation potential. In addition, the pronounced chondrogenic potential of IPFP-ASCs makes them promising candidates for research for use in other methods of regenerative therapy. The purpose of this study was to ascertain the presence and compare the relative abundance of cells exhibiting an immunohistochemical profile characteristic of adipose-derived mesenchymal stem cells in selected samples of the stromal vascular fraction (SVF) obtained from the IPFP and subcutaneous fat tissue.
A direct immunohistochemical study was carried out in serial paraffin sections of the SVF of the infrapatellar fat pad (IPFP) and subcutaneous tissue, using monoclonal antibodies. The minimum criteria were established by the International Society for Cell Therapy to ensure the identity of mesenchymal stem cells use CD73, CD90, and CD105 as positive markers and CD34, CD31, and CD45 as a negative.
According to the results of histological, immunohistochemical, morphometric, and statistical studies, it was found that in the SVF of IPFP and subcutaneous adipose tissue, the relative number of cells with the profile CD105+, CD73+, CD34+, CD31−, CD45− in the standard field of view (×200), the SVF of IPFP was 1.58%, whereas the SVF of subcutaneous adipose tissue was 6.92 %, which was statistically significantly greater by 4.38 times (p |
| doi_str_mv | 10.1016/j.jisako.2024.05.011 |
| format | Article |
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A direct immunohistochemical study was carried out in serial paraffin sections of the SVF of the infrapatellar fat pad (IPFP) and subcutaneous tissue, using monoclonal antibodies. The minimum criteria were established by the International Society for Cell Therapy to ensure the identity of mesenchymal stem cells use CD73, CD90, and CD105 as positive markers and CD34, CD31, and CD45 as a negative.
According to the results of histological, immunohistochemical, morphometric, and statistical studies, it was found that in the SVF of IPFP and subcutaneous adipose tissue, the relative number of cells with the profile CD105+, CD73+, CD34+, CD31−, CD45− in the standard field of view (×200), the SVF of IPFP was 1.58%, whereas the SVF of subcutaneous adipose tissue was 6.92 %, which was statistically significantly greater by 4.38 times (p < 0.05).
The presence of a sufficient number of mesenchymal stromal cells in IPFP in combination with their topographic relationship with the structures of the joint determines the use of the SVF of the IPFP for the treatment of diseases of the knee joint.
III.</description><identifier>ISSN: 2059-7754</identifier><identifier>ISSN: 2059-7762</identifier><identifier>EISSN: 2059-7762</identifier><identifier>DOI: 10.1016/j.jisako.2024.05.011</identifier><identifier>PMID: 38763383</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>5'-Nucleotidase - metabolism ; Adipose Tissue - cytology ; Adult ; Aged ; Antigens, CD - metabolism ; Cell Differentiation ; Endoglin - metabolism ; Female ; GPI-Linked Proteins ; Humans ; Immunohistochemistry - methods ; Infrapatellar fat pad ; Male ; Mesenchymal stem cells ; Mesenchymal Stem Cells - cytology ; Middle Aged ; Patella - cytology ; Regeneration ; Regenerative ; Stromal Vascular Fraction ; Subcutaneous Fat - cytology ; Thy-1 Antigens - metabolism</subject><ispartof>Journal of ISAKOS, 2024-08, Vol.9 (4), p.615-619</ispartof><rights>2024 The Author(s)</rights><rights>Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c357t-e063347c7f22d26b594dd70831b112fc85643618571ba2eb098a0024eef78bca3</cites><orcidid>0009-0008-6827-3001 ; 0000-0002-7505-8587 ; 0000-0003-2835-9333 ; 0000-0001-8267-2350</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38763383$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maslennikov, Serhii</creatorcontrib><creatorcontrib>Avramenko, Yuliia</creatorcontrib><creatorcontrib>Tumanskiy, Valeriy</creatorcontrib><creatorcontrib>Golovakha, Maksym</creatorcontrib><title>Comparative characteristics of the stem cells’ number in the stromal vascular fraction of infrapatellar fat pad and subcutaneous fat tissue</title><title>Journal of ISAKOS</title><addtitle>J ISAKOS</addtitle><description>The use of infrapatellar fat pad adipose stem cells (IPFP-ASCs) shows an age-independent proliferation and differentiation potential. In addition, the pronounced chondrogenic potential of IPFP-ASCs makes them promising candidates for research for use in other methods of regenerative therapy. The purpose of this study was to ascertain the presence and compare the relative abundance of cells exhibiting an immunohistochemical profile characteristic of adipose-derived mesenchymal stem cells in selected samples of the stromal vascular fraction (SVF) obtained from the IPFP and subcutaneous fat tissue.
A direct immunohistochemical study was carried out in serial paraffin sections of the SVF of the infrapatellar fat pad (IPFP) and subcutaneous tissue, using monoclonal antibodies. The minimum criteria were established by the International Society for Cell Therapy to ensure the identity of mesenchymal stem cells use CD73, CD90, and CD105 as positive markers and CD34, CD31, and CD45 as a negative.
According to the results of histological, immunohistochemical, morphometric, and statistical studies, it was found that in the SVF of IPFP and subcutaneous adipose tissue, the relative number of cells with the profile CD105+, CD73+, CD34+, CD31−, CD45− in the standard field of view (×200), the SVF of IPFP was 1.58%, whereas the SVF of subcutaneous adipose tissue was 6.92 %, which was statistically significantly greater by 4.38 times (p < 0.05).
The presence of a sufficient number of mesenchymal stromal cells in IPFP in combination with their topographic relationship with the structures of the joint determines the use of the SVF of the IPFP for the treatment of diseases of the knee joint.
III.</description><subject>5'-Nucleotidase - metabolism</subject><subject>Adipose Tissue - cytology</subject><subject>Adult</subject><subject>Aged</subject><subject>Antigens, CD - metabolism</subject><subject>Cell Differentiation</subject><subject>Endoglin - metabolism</subject><subject>Female</subject><subject>GPI-Linked Proteins</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Infrapatellar fat pad</subject><subject>Male</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Middle Aged</subject><subject>Patella - cytology</subject><subject>Regeneration</subject><subject>Regenerative</subject><subject>Stromal Vascular Fraction</subject><subject>Subcutaneous Fat - cytology</subject><subject>Thy-1 Antigens - metabolism</subject><issn>2059-7754</issn><issn>2059-7762</issn><issn>2059-7762</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uctu1DAUtRAVrUr_oEJespngRxxnNkhoxEuqxAbWlu3cqB6SOPg6I7HjB_iA_h5fgkOmXbLytc4593EOIbecVZzx5s2xOga032MlmKgrpirG-TNyJZja77RuxPOnWtWX5AYxOKaYllIr_oJcylY3Urbyivw-xHG2yeZwAurvS-UzpIA5eKSxp_keKGYYqYdhwD-_Hui0jA4SDdMZS3G0Az1Z9MtgE-3XDiFOqzhM5TfbXKQrYjOdbUft1FFcnF-ynSAu-A_IAXGBl-SitwPCzfm9Jt8-vP96-LS7-_Lx8-Hd3c5LpfMOWNm-1l73QnSicWpfd51mreSOc9H7VjW1bHirNHdWgGP71rJiFECvW-etvCavt75zij8WwGzGgOuF20ZGMqWLW_tGFWq9UX2KiAl6M6cw2vTTcGbWLMzRbFmYNQvDlClZFNmr84TFjdA9iR6dL4S3GwHKnacAyaAPMHnoQgKfTRfD_yf8BTGsn-g</recordid><startdate>20240801</startdate><enddate>20240801</enddate><creator>Maslennikov, Serhii</creator><creator>Avramenko, Yuliia</creator><creator>Tumanskiy, Valeriy</creator><creator>Golovakha, Maksym</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0008-6827-3001</orcidid><orcidid>https://orcid.org/0000-0002-7505-8587</orcidid><orcidid>https://orcid.org/0000-0003-2835-9333</orcidid><orcidid>https://orcid.org/0000-0001-8267-2350</orcidid></search><sort><creationdate>20240801</creationdate><title>Comparative characteristics of the stem cells’ number in the stromal vascular fraction of infrapatellar fat pad and subcutaneous fat tissue</title><author>Maslennikov, Serhii ; Avramenko, Yuliia ; Tumanskiy, Valeriy ; Golovakha, Maksym</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-e063347c7f22d26b594dd70831b112fc85643618571ba2eb098a0024eef78bca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>5'-Nucleotidase - metabolism</topic><topic>Adipose Tissue - cytology</topic><topic>Adult</topic><topic>Aged</topic><topic>Antigens, CD - metabolism</topic><topic>Cell Differentiation</topic><topic>Endoglin - metabolism</topic><topic>Female</topic><topic>GPI-Linked Proteins</topic><topic>Humans</topic><topic>Immunohistochemistry - methods</topic><topic>Infrapatellar fat pad</topic><topic>Male</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Middle Aged</topic><topic>Patella - cytology</topic><topic>Regeneration</topic><topic>Regenerative</topic><topic>Stromal Vascular Fraction</topic><topic>Subcutaneous Fat - cytology</topic><topic>Thy-1 Antigens - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maslennikov, Serhii</creatorcontrib><creatorcontrib>Avramenko, Yuliia</creatorcontrib><creatorcontrib>Tumanskiy, Valeriy</creatorcontrib><creatorcontrib>Golovakha, Maksym</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ISAKOS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maslennikov, Serhii</au><au>Avramenko, Yuliia</au><au>Tumanskiy, Valeriy</au><au>Golovakha, Maksym</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative characteristics of the stem cells’ number in the stromal vascular fraction of infrapatellar fat pad and subcutaneous fat tissue</atitle><jtitle>Journal of ISAKOS</jtitle><addtitle>J ISAKOS</addtitle><date>2024-08-01</date><risdate>2024</risdate><volume>9</volume><issue>4</issue><spage>615</spage><epage>619</epage><pages>615-619</pages><issn>2059-7754</issn><issn>2059-7762</issn><eissn>2059-7762</eissn><abstract>The use of infrapatellar fat pad adipose stem cells (IPFP-ASCs) shows an age-independent proliferation and differentiation potential. In addition, the pronounced chondrogenic potential of IPFP-ASCs makes them promising candidates for research for use in other methods of regenerative therapy. The purpose of this study was to ascertain the presence and compare the relative abundance of cells exhibiting an immunohistochemical profile characteristic of adipose-derived mesenchymal stem cells in selected samples of the stromal vascular fraction (SVF) obtained from the IPFP and subcutaneous fat tissue.
A direct immunohistochemical study was carried out in serial paraffin sections of the SVF of the infrapatellar fat pad (IPFP) and subcutaneous tissue, using monoclonal antibodies. The minimum criteria were established by the International Society for Cell Therapy to ensure the identity of mesenchymal stem cells use CD73, CD90, and CD105 as positive markers and CD34, CD31, and CD45 as a negative.
According to the results of histological, immunohistochemical, morphometric, and statistical studies, it was found that in the SVF of IPFP and subcutaneous adipose tissue, the relative number of cells with the profile CD105+, CD73+, CD34+, CD31−, CD45− in the standard field of view (×200), the SVF of IPFP was 1.58%, whereas the SVF of subcutaneous adipose tissue was 6.92 %, which was statistically significantly greater by 4.38 times (p < 0.05).
The presence of a sufficient number of mesenchymal stromal cells in IPFP in combination with their topographic relationship with the structures of the joint determines the use of the SVF of the IPFP for the treatment of diseases of the knee joint.
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| subjects | 5'-Nucleotidase - metabolism Adipose Tissue - cytology Adult Aged Antigens, CD - metabolism Cell Differentiation Endoglin - metabolism Female GPI-Linked Proteins Humans Immunohistochemistry - methods Infrapatellar fat pad Male Mesenchymal stem cells Mesenchymal Stem Cells - cytology Middle Aged Patella - cytology Regeneration Regenerative Stromal Vascular Fraction Subcutaneous Fat - cytology Thy-1 Antigens - metabolism |
| title | Comparative characteristics of the stem cells’ number in the stromal vascular fraction of infrapatellar fat pad and subcutaneous fat tissue |
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