Effects of blood-processing protocols on cell-free DNA fragmentomics in plasma: Comparisons of one- and two-step centrifugations

•A single low-speed centrifugation is a simple and quick option for cfDNA research.•One-step protocol produces reliable results on the nuclear cfDNA fragmentomics.•The fragmentomics of cell-free mtDNA are impacted by centrifugation protocols.•One-step protocol is feasible to detect cfDNA size shorte...

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