Preparation of hollow double-layer Pt@CeO2 nanospheres as oxidase mimetics for the colorimetric-fluorescent-SERS triple-mode detection of glutathione in serum

Glutathione (GSH) is an essential antioxidant in the human body, but its detection is difficult due to the interference of complex components in serum. Herein, hollow double-layer Pt@CeO2 nanospheres were developed as oxidase mimetics, and the light-assisted oxidase mimetics effects were found. The oxidase activity was enhanced significantly by utilizing the synergistic effect of Schottky junction and the localized surface plasmon resonance (LSPR) of Pt under UV light. A novel GSH colorimetric-fluorescent-SERS sensing platform was established, with the sensing performance notably boosted by using the light-assisted oxidase mimetics effects. This platform boasts an exceptionally low detection limit (LOD) of 0.084 μM, while the detection time was shortened from 10 min to just 2 min. The anti-interference detection with high recovery rate (96.84%–107.4 %) in real serum made it be promising for practical application. HD-Pt@CeO2 nanospheres were successfully prepared as oxidase mimetics for colorimetric-fluorescent-SERS triple-mode detection of glutathione. The oxidase activity was enhanced significantly by using the synergistic effect of Schottky junction and the LSPR of Pt under UV light. The light-assisted oxidase mimetics effects achieved the enhanced sensing performance with the reduced LOD (0.084 μM) and shorted detection time (2 min). [Display omitted] •HD-Pt@CeO2 were developed as oxidase mimetics.•The oxidase-like activity was enhanced by the light-assisted nano-enzyme effects.•A colorimetric-fluorescent-SERS triple-mode sensing platform was established.•Sensitivity was significantly improved and detection time was obviously shortened.•The high recovery rate made it be promising for application in serum detection.