Multiplex CRISPR-Cas Genome Editing: Next-Generation Microbial Strain Engineering

Multiplex CRISPR-Cas Genome Editing: Next-Generation Microbial Strain Engineering

Genome editing is a crucial technology for obtaining desired phenotypes in a variety of species, ranging from microbes to plants, animals, and humans. With the advent of CRISPR-Cas technology, it has become possible to edit the intended sequence by modifying the target recognition sequence in guide...

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Veröffentlicht in:Journal of agricultural and food chemistry 2024-05, Vol.72 (21), p.11871-11884
Hauptverfasser: Lim, Se Ra, Lee, Sang Jun
Format: Artikel
Sprache:eng
Schlagworte:
Bacteria - classification
Bacteria - genetics
Bacteria - metabolism
CRISPR-Cas Systems
Gene Editing - methods
Yeasts - genetics
Yeasts - metabolism
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container_title Journal of agricultural and food chemistry
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creator Lim, Se Ra
Lee, Sang Jun
description Genome editing is a crucial technology for obtaining desired phenotypes in a variety of species, ranging from microbes to plants, animals, and humans. With the advent of CRISPR-Cas technology, it has become possible to edit the intended sequence by modifying the target recognition sequence in guide RNA (gRNA). By expressing multiple gRNAs simultaneously, it is possible to edit multiple targets at the same time, allowing for the simultaneous introduction of various functions into the cell. This can significantly reduce the time and cost of obtaining engineered microbial strains for specific traits. In this review, we investigate the resolution of multiplex genome editing and its application in engineering microorganisms, including bacteria and yeast. Furthermore, we examine how recent advancements in artificial intelligence technology could assist in microbial genome editing and engineering. Based on these insights, we present our perspectives on the future evolution and potential impact of multiplex genome editing technologies in the agriculture and food industry.
doi_str_mv 10.1021/acs.jafc.4c01650
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subjects Bacteria - classification
Bacteria - genetics
Bacteria - metabolism
CRISPR-Cas Systems
Gene Editing - methods
Yeasts - genetics
Yeasts - metabolism
title Multiplex CRISPR-Cas Genome Editing: Next-Generation Microbial Strain Engineering
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