Effects of garlic‐derived fructan and oligofructose mixtures on intestinal health and constipation relief in mice
BACKGROUND Garlic polysaccharides (GPs) constitute over 75% of the dry weight of garlic. They are characterized by fructan with a 2,1‐β‐d‐Fruf backbone and 2,6‐β‐d‐Fruf branches. Studies have suggested a role for GPs in regulating gut microbiota but whether they possess a comprehensive function in m...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2024-09, Vol.104 (12), p.7476-7487 |
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| creator | Xie, Chanyuan Gao, Wei Liang, Xingdi Chye, Fook Yee |
| description | BACKGROUND
Garlic polysaccharides (GPs) constitute over 75% of the dry weight of garlic. They are characterized by fructan with a 2,1‐β‐d‐Fruf backbone and 2,6‐β‐d‐Fruf branches. Studies have suggested a role for GPs in regulating gut microbiota but whether they possess a comprehensive function in maintaining intestinal well‐being and can serve as effective prebiotics remains unknown. To explore this, varied doses of GPs (1.25–5.0 g kg−1 body weight) and inulin (as a positive control) were administered to Kunming mice via gavage, and their effects on the intestinal epithelial, chemical, and biological barriers were assessed. A constipation model was also established using loperamide to investigate the potential effects of GPs on the relief of constipation.
RESULTS
Administration of GPs significantly upregulated expression of tight‐junction proteins and mucins in Kunming mouse small‐intestine tissue. Garlic polysaccharides elevated cecal butyric acid content, reduced the abundance of Desulfobacterota, and decreased the ratio of Firmicutes to Bacteroidetes (the F/B ratio). Garlic polysaccharides also promoted the growth of Bacteroides acidifaciens and Clostridium saccharogumia. Tax4Fun functional predictions suggested the potential of GPs to prevent human diseases, reducing the risk of insulin resistance, infectious diseases, and drug resistance. Garlic polysaccharides also exhibited a beneficial effect in alleviating loperamide‐induced constipation symptoms by enhancing small intestinal transit, softening stool consistency, accelerating bowel movements, and promoting the release of excitatory neurotransmitters.
CONCLUSIONS
These findings highlight the important role of GPs in maintaining gut fitness by enhancing intestinal barrier function and peristalsis. Garlic polysaccharides are promising prebiotics, potentially contributing to overall intestinal well‐being and health. © 2024 Society of Chemical Industry. |
| doi_str_mv | 10.1002/jsfa.13567 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3055449906</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3089064402</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3167-bf972e0a1143aac0896a9e78a46a73e8f7890e49e7c034d65f47430a62a6a3ce3</originalsourceid><addsrcrecordid>eNp90c1u1DAQB3CrArXbj0sfAEXigiqljGPHjo9V1fKhShyg52jqjFuvvPFiJ4XeeASekSfBu1s4cOBkafybvz-GsVMO5xygebvMDs-5aJXeYwsORtcAHF6wRdls6pbL5oAd5rwEAGOU2mcHotOyaaVasHzlHNkpV9FV95iCt79-_Bwo-UcaKpdmO-FY4ThUMfj7uC3ETNXKf5_mRKVtrPw4UZ78iKF6IAzTw9bbOJbiGidfSKLgyRVZGi0ds5cOQ6aT5_WI3V5ffbl8X998evfh8uKmtoIrXd85oxsC5FwKRAudUWhIdygVakGd050BkqVkQchBtU5qKQBVgwqFJXHE3uxy1yl-ncsd-5XPlkLAkeKcewFtK6UxoAp9_Q9dxjmVJ21UOUZJCU1RZztlU8w5kevXya8wPfUc-s0o-s0o-u0oCn71HDnfrWj4S__8fQF8B775QE__ieo_fr6-2IX-Bg_mlZk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3089064402</pqid></control><display><type>article</type><title>Effects of garlic‐derived fructan and oligofructose mixtures on intestinal health and constipation relief in mice</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Xie, Chanyuan ; Gao, Wei ; Liang, Xingdi ; Chye, Fook Yee</creator><creatorcontrib>Xie, Chanyuan ; Gao, Wei ; Liang, Xingdi ; Chye, Fook Yee</creatorcontrib><description>BACKGROUND
Garlic polysaccharides (GPs) constitute over 75% of the dry weight of garlic. They are characterized by fructan with a 2,1‐β‐d‐Fruf backbone and 2,6‐β‐d‐Fruf branches. Studies have suggested a role for GPs in regulating gut microbiota but whether they possess a comprehensive function in maintaining intestinal well‐being and can serve as effective prebiotics remains unknown. To explore this, varied doses of GPs (1.25–5.0 g kg−1 body weight) and inulin (as a positive control) were administered to Kunming mice via gavage, and their effects on the intestinal epithelial, chemical, and biological barriers were assessed. A constipation model was also established using loperamide to investigate the potential effects of GPs on the relief of constipation.
RESULTS
Administration of GPs significantly upregulated expression of tight‐junction proteins and mucins in Kunming mouse small‐intestine tissue. Garlic polysaccharides elevated cecal butyric acid content, reduced the abundance of Desulfobacterota, and decreased the ratio of Firmicutes to Bacteroidetes (the F/B ratio). Garlic polysaccharides also promoted the growth of Bacteroides acidifaciens and Clostridium saccharogumia. Tax4Fun functional predictions suggested the potential of GPs to prevent human diseases, reducing the risk of insulin resistance, infectious diseases, and drug resistance. Garlic polysaccharides also exhibited a beneficial effect in alleviating loperamide‐induced constipation symptoms by enhancing small intestinal transit, softening stool consistency, accelerating bowel movements, and promoting the release of excitatory neurotransmitters.
CONCLUSIONS
These findings highlight the important role of GPs in maintaining gut fitness by enhancing intestinal barrier function and peristalsis. Garlic polysaccharides are promising prebiotics, potentially contributing to overall intestinal well‐being and health. © 2024 Society of Chemical Industry.</description><identifier>ISSN: 0022-5142</identifier><identifier>ISSN: 1097-0010</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.13567</identifier><identifier>PMID: 38742546</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Acidification ; Biological effects ; Body weight ; Butyric acid ; Cecum ; Constipation ; Digestive system ; Drug resistance ; Fructooligosaccharides ; Garlic ; garlic polysaccharides ; Gastrointestinal tract ; Infectious diseases ; Insulin resistance ; intestinal barrier ; Intestinal microflora ; Intestine ; Mucin ; Mucins ; neurotransmitters ; Peristalsis ; Polysaccharides ; Prebiotics ; Saccharides ; short‐chain fatty acids ; Small intestine</subject><ispartof>Journal of the science of food and agriculture, 2024-09, Vol.104 (12), p.7476-7487</ispartof><rights>2024 Society of Chemical Industry.</rights><rights>2024 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3167-bf972e0a1143aac0896a9e78a46a73e8f7890e49e7c034d65f47430a62a6a3ce3</cites><orcidid>0000-0002-6886-3721 ; 0009-0008-9887-7738 ; 0000-0001-8834-8973</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.13567$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.13567$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38742546$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Chanyuan</creatorcontrib><creatorcontrib>Gao, Wei</creatorcontrib><creatorcontrib>Liang, Xingdi</creatorcontrib><creatorcontrib>Chye, Fook Yee</creatorcontrib><title>Effects of garlic‐derived fructan and oligofructose mixtures on intestinal health and constipation relief in mice</title><title>Journal of the science of food and agriculture</title><addtitle>J Sci Food Agric</addtitle><description>BACKGROUND
Garlic polysaccharides (GPs) constitute over 75% of the dry weight of garlic. They are characterized by fructan with a 2,1‐β‐d‐Fruf backbone and 2,6‐β‐d‐Fruf branches. Studies have suggested a role for GPs in regulating gut microbiota but whether they possess a comprehensive function in maintaining intestinal well‐being and can serve as effective prebiotics remains unknown. To explore this, varied doses of GPs (1.25–5.0 g kg−1 body weight) and inulin (as a positive control) were administered to Kunming mice via gavage, and their effects on the intestinal epithelial, chemical, and biological barriers were assessed. A constipation model was also established using loperamide to investigate the potential effects of GPs on the relief of constipation.
RESULTS
Administration of GPs significantly upregulated expression of tight‐junction proteins and mucins in Kunming mouse small‐intestine tissue. Garlic polysaccharides elevated cecal butyric acid content, reduced the abundance of Desulfobacterota, and decreased the ratio of Firmicutes to Bacteroidetes (the F/B ratio). Garlic polysaccharides also promoted the growth of Bacteroides acidifaciens and Clostridium saccharogumia. Tax4Fun functional predictions suggested the potential of GPs to prevent human diseases, reducing the risk of insulin resistance, infectious diseases, and drug resistance. Garlic polysaccharides also exhibited a beneficial effect in alleviating loperamide‐induced constipation symptoms by enhancing small intestinal transit, softening stool consistency, accelerating bowel movements, and promoting the release of excitatory neurotransmitters.
CONCLUSIONS
These findings highlight the important role of GPs in maintaining gut fitness by enhancing intestinal barrier function and peristalsis. Garlic polysaccharides are promising prebiotics, potentially contributing to overall intestinal well‐being and health. © 2024 Society of Chemical Industry.</description><subject>Acidification</subject><subject>Biological effects</subject><subject>Body weight</subject><subject>Butyric acid</subject><subject>Cecum</subject><subject>Constipation</subject><subject>Digestive system</subject><subject>Drug resistance</subject><subject>Fructooligosaccharides</subject><subject>Garlic</subject><subject>garlic polysaccharides</subject><subject>Gastrointestinal tract</subject><subject>Infectious diseases</subject><subject>Insulin resistance</subject><subject>intestinal barrier</subject><subject>Intestinal microflora</subject><subject>Intestine</subject><subject>Mucin</subject><subject>Mucins</subject><subject>neurotransmitters</subject><subject>Peristalsis</subject><subject>Polysaccharides</subject><subject>Prebiotics</subject><subject>Saccharides</subject><subject>short‐chain fatty acids</subject><subject>Small intestine</subject><issn>0022-5142</issn><issn>1097-0010</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp90c1u1DAQB3CrArXbj0sfAEXigiqljGPHjo9V1fKhShyg52jqjFuvvPFiJ4XeeASekSfBu1s4cOBkafybvz-GsVMO5xygebvMDs-5aJXeYwsORtcAHF6wRdls6pbL5oAd5rwEAGOU2mcHotOyaaVasHzlHNkpV9FV95iCt79-_Bwo-UcaKpdmO-FY4ThUMfj7uC3ETNXKf5_mRKVtrPw4UZ78iKF6IAzTw9bbOJbiGidfSKLgyRVZGi0ds5cOQ6aT5_WI3V5ffbl8X998evfh8uKmtoIrXd85oxsC5FwKRAudUWhIdygVakGd050BkqVkQchBtU5qKQBVgwqFJXHE3uxy1yl-ncsd-5XPlkLAkeKcewFtK6UxoAp9_Q9dxjmVJ21UOUZJCU1RZztlU8w5kevXya8wPfUc-s0o-s0o-u0oCn71HDnfrWj4S__8fQF8B775QE__ieo_fr6-2IX-Bg_mlZk</recordid><startdate>202409</startdate><enddate>202409</enddate><creator>Xie, Chanyuan</creator><creator>Gao, Wei</creator><creator>Liang, Xingdi</creator><creator>Chye, Fook Yee</creator><general>John Wiley & Sons, Ltd</general><general>John Wiley and Sons, Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6886-3721</orcidid><orcidid>https://orcid.org/0009-0008-9887-7738</orcidid><orcidid>https://orcid.org/0000-0001-8834-8973</orcidid></search><sort><creationdate>202409</creationdate><title>Effects of garlic‐derived fructan and oligofructose mixtures on intestinal health and constipation relief in mice</title><author>Xie, Chanyuan ; Gao, Wei ; Liang, Xingdi ; Chye, Fook Yee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3167-bf972e0a1143aac0896a9e78a46a73e8f7890e49e7c034d65f47430a62a6a3ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acidification</topic><topic>Biological effects</topic><topic>Body weight</topic><topic>Butyric acid</topic><topic>Cecum</topic><topic>Constipation</topic><topic>Digestive system</topic><topic>Drug resistance</topic><topic>Fructooligosaccharides</topic><topic>Garlic</topic><topic>garlic polysaccharides</topic><topic>Gastrointestinal tract</topic><topic>Infectious diseases</topic><topic>Insulin resistance</topic><topic>intestinal barrier</topic><topic>Intestinal microflora</topic><topic>Intestine</topic><topic>Mucin</topic><topic>Mucins</topic><topic>neurotransmitters</topic><topic>Peristalsis</topic><topic>Polysaccharides</topic><topic>Prebiotics</topic><topic>Saccharides</topic><topic>short‐chain fatty acids</topic><topic>Small intestine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Chanyuan</creatorcontrib><creatorcontrib>Gao, Wei</creatorcontrib><creatorcontrib>Liang, Xingdi</creatorcontrib><creatorcontrib>Chye, Fook Yee</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Chanyuan</au><au>Gao, Wei</au><au>Liang, Xingdi</au><au>Chye, Fook Yee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of garlic‐derived fructan and oligofructose mixtures on intestinal health and constipation relief in mice</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J Sci Food Agric</addtitle><date>2024-09</date><risdate>2024</risdate><volume>104</volume><issue>12</issue><spage>7476</spage><epage>7487</epage><pages>7476-7487</pages><issn>0022-5142</issn><issn>1097-0010</issn><eissn>1097-0010</eissn><abstract>BACKGROUND
Garlic polysaccharides (GPs) constitute over 75% of the dry weight of garlic. They are characterized by fructan with a 2,1‐β‐d‐Fruf backbone and 2,6‐β‐d‐Fruf branches. Studies have suggested a role for GPs in regulating gut microbiota but whether they possess a comprehensive function in maintaining intestinal well‐being and can serve as effective prebiotics remains unknown. To explore this, varied doses of GPs (1.25–5.0 g kg−1 body weight) and inulin (as a positive control) were administered to Kunming mice via gavage, and their effects on the intestinal epithelial, chemical, and biological barriers were assessed. A constipation model was also established using loperamide to investigate the potential effects of GPs on the relief of constipation.
RESULTS
Administration of GPs significantly upregulated expression of tight‐junction proteins and mucins in Kunming mouse small‐intestine tissue. Garlic polysaccharides elevated cecal butyric acid content, reduced the abundance of Desulfobacterota, and decreased the ratio of Firmicutes to Bacteroidetes (the F/B ratio). Garlic polysaccharides also promoted the growth of Bacteroides acidifaciens and Clostridium saccharogumia. Tax4Fun functional predictions suggested the potential of GPs to prevent human diseases, reducing the risk of insulin resistance, infectious diseases, and drug resistance. Garlic polysaccharides also exhibited a beneficial effect in alleviating loperamide‐induced constipation symptoms by enhancing small intestinal transit, softening stool consistency, accelerating bowel movements, and promoting the release of excitatory neurotransmitters.
CONCLUSIONS
These findings highlight the important role of GPs in maintaining gut fitness by enhancing intestinal barrier function and peristalsis. Garlic polysaccharides are promising prebiotics, potentially contributing to overall intestinal well‐being and health. © 2024 Society of Chemical Industry.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>38742546</pmid><doi>10.1002/jsfa.13567</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-6886-3721</orcidid><orcidid>https://orcid.org/0009-0008-9887-7738</orcidid><orcidid>https://orcid.org/0000-0001-8834-8973</orcidid></addata></record> |
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| subjects | Acidification Biological effects Body weight Butyric acid Cecum Constipation Digestive system Drug resistance Fructooligosaccharides Garlic garlic polysaccharides Gastrointestinal tract Infectious diseases Insulin resistance intestinal barrier Intestinal microflora Intestine Mucin Mucins neurotransmitters Peristalsis Polysaccharides Prebiotics Saccharides short‐chain fatty acids Small intestine |
| title | Effects of garlic‐derived fructan and oligofructose mixtures on intestinal health and constipation relief in mice |
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