A reproducible and affordable method of conducting luciferase assay
Commercially available glow luciferase assay kits are widely popular and convenient to use. However, concerning high-throughput screening, commercial kits are limited by huge running costs. As an alternative to commercial luciferase assay kits, this study presents a cost-effective and efficient meth...
Gespeichert in:
| Veröffentlicht in: | Analytical biochemistry 2024-09, Vol.692, p.115558, Article 115558 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | 115558 |
| container_title | Analytical biochemistry |
| container_volume | 692 |
| creator | Reddy, Kirtana Ramachandran, Balaji |
| description | Commercially available glow luciferase assay kits are widely popular and convenient to use. However, concerning high-throughput screening, commercial kits are limited by huge running costs. As an alternative to commercial luciferase assay kits, this study presents a cost-effective and efficient methodology of performing a simple and rapid laboratory flash luciferase assay. The proposed luciferase assay method has a versatile use ranging from screening lysates in a microplate reader for quantitative assay as well as screening live cells qualitatively or quantitatively under an imaging system.
[Display omitted]
•This study demonstrates a reliable, and reproducible alternative to commercial luciferase assay kits.•Can be used for high-throughput luciferase activity screening due to its cost-effectiveness.•Proposes a composition and combination of luciferase assay buffers and lysis buffers for efficient screening.•This assay can be applied for quantitative cell-lysate screening for luciferase activity in microplate readers.•Can be applied to qualitative or quantitative screening of luciferase assay in live –cells using an imaging system. |
| doi_str_mv | 10.1016/j.ab.2024.115558 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3054433686</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0003269724001027</els_id><sourcerecordid>3054433686</sourcerecordid><originalsourceid>FETCH-LOGICAL-c303t-370124fbe391b08d6fe9636c2f146455d2213def07d1ad3c3f28d33f3aa1660c3</originalsourceid><addsrcrecordid>eNp1kD1PwzAQhi0EoqWwM6GMLAlnX-IkbFXFl1SJBWbLsc-QKk2KnSD135MqhY3pdNLzvrp7GLvmkHDg8m6T6CoRINKE8yzLihM251DKGBDKUzYHAIyFLPMZuwhhA8B5mslzNsMixywVcs5Wy8jTznd2MHXVUKRbG2nnOm_1Yd1S_9nZqHOR6dqR6ev2I2pG1pHXYcRD0PtLduZ0E-jqOBfs_fHhbfUcr1-fXlbLdWwQsI8xBy5SVxGWvILCSkelRGmE46lMs8wKwdGSg9xybdGgE4VFdKg1lxIMLtjt1Dve-zVQ6NW2DoaaRrfUDUEhZGmKKAs5ojChxncheHJq5-ut9nvFQR3UqY3SlTqoU5O6MXJzbB-qLdm_wK-rEbifABp__K7Jq2Bqag3Z2pPple3q_9t_ANtOfK0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3054433686</pqid></control><display><type>article</type><title>A reproducible and affordable method of conducting luciferase assay</title><source>Access via ScienceDirect (Elsevier)</source><creator>Reddy, Kirtana ; Ramachandran, Balaji</creator><creatorcontrib>Reddy, Kirtana ; Ramachandran, Balaji</creatorcontrib><description>Commercially available glow luciferase assay kits are widely popular and convenient to use. However, concerning high-throughput screening, commercial kits are limited by huge running costs. As an alternative to commercial luciferase assay kits, this study presents a cost-effective and efficient methodology of performing a simple and rapid laboratory flash luciferase assay. The proposed luciferase assay method has a versatile use ranging from screening lysates in a microplate reader for quantitative assay as well as screening live cells qualitatively or quantitatively under an imaging system.
[Display omitted]
•This study demonstrates a reliable, and reproducible alternative to commercial luciferase assay kits.•Can be used for high-throughput luciferase activity screening due to its cost-effectiveness.•Proposes a composition and combination of luciferase assay buffers and lysis buffers for efficient screening.•This assay can be applied for quantitative cell-lysate screening for luciferase activity in microplate readers.•Can be applied to qualitative or quantitative screening of luciferase assay in live –cells using an imaging system.</description><identifier>ISSN: 0003-2697</identifier><identifier>ISSN: 1096-0309</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2024.115558</identifier><identifier>PMID: 38735426</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Dual-Luciferase assay ; Firefly luciferase assay buffer ; Luciferin and coelenterazine ; Passive lysis buffer ; Renilla luciferase assay buffer</subject><ispartof>Analytical biochemistry, 2024-09, Vol.692, p.115558, Article 115558</ispartof><rights>2024 Elsevier Inc.</rights><rights>Copyright © 2024. Published by Elsevier Inc.</rights><rights>Copyright © 2024 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c303t-370124fbe391b08d6fe9636c2f146455d2213def07d1ad3c3f28d33f3aa1660c3</cites><orcidid>0000-0003-1882-9717</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ab.2024.115558$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38735426$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reddy, Kirtana</creatorcontrib><creatorcontrib>Ramachandran, Balaji</creatorcontrib><title>A reproducible and affordable method of conducting luciferase assay</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Commercially available glow luciferase assay kits are widely popular and convenient to use. However, concerning high-throughput screening, commercial kits are limited by huge running costs. As an alternative to commercial luciferase assay kits, this study presents a cost-effective and efficient methodology of performing a simple and rapid laboratory flash luciferase assay. The proposed luciferase assay method has a versatile use ranging from screening lysates in a microplate reader for quantitative assay as well as screening live cells qualitatively or quantitatively under an imaging system.
[Display omitted]
•This study demonstrates a reliable, and reproducible alternative to commercial luciferase assay kits.•Can be used for high-throughput luciferase activity screening due to its cost-effectiveness.•Proposes a composition and combination of luciferase assay buffers and lysis buffers for efficient screening.•This assay can be applied for quantitative cell-lysate screening for luciferase activity in microplate readers.•Can be applied to qualitative or quantitative screening of luciferase assay in live –cells using an imaging system.</description><subject>Dual-Luciferase assay</subject><subject>Firefly luciferase assay buffer</subject><subject>Luciferin and coelenterazine</subject><subject>Passive lysis buffer</subject><subject>Renilla luciferase assay buffer</subject><issn>0003-2697</issn><issn>1096-0309</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp1kD1PwzAQhi0EoqWwM6GMLAlnX-IkbFXFl1SJBWbLsc-QKk2KnSD135MqhY3pdNLzvrp7GLvmkHDg8m6T6CoRINKE8yzLihM251DKGBDKUzYHAIyFLPMZuwhhA8B5mslzNsMixywVcs5Wy8jTznd2MHXVUKRbG2nnOm_1Yd1S_9nZqHOR6dqR6ev2I2pG1pHXYcRD0PtLduZ0E-jqOBfs_fHhbfUcr1-fXlbLdWwQsI8xBy5SVxGWvILCSkelRGmE46lMs8wKwdGSg9xybdGgE4VFdKg1lxIMLtjt1Dve-zVQ6NW2DoaaRrfUDUEhZGmKKAs5ojChxncheHJq5-ut9nvFQR3UqY3SlTqoU5O6MXJzbB-qLdm_wK-rEbifABp__K7Jq2Bqag3Z2pPple3q_9t_ANtOfK0</recordid><startdate>20240901</startdate><enddate>20240901</enddate><creator>Reddy, Kirtana</creator><creator>Ramachandran, Balaji</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1882-9717</orcidid></search><sort><creationdate>20240901</creationdate><title>A reproducible and affordable method of conducting luciferase assay</title><author>Reddy, Kirtana ; Ramachandran, Balaji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-370124fbe391b08d6fe9636c2f146455d2213def07d1ad3c3f28d33f3aa1660c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Dual-Luciferase assay</topic><topic>Firefly luciferase assay buffer</topic><topic>Luciferin and coelenterazine</topic><topic>Passive lysis buffer</topic><topic>Renilla luciferase assay buffer</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reddy, Kirtana</creatorcontrib><creatorcontrib>Ramachandran, Balaji</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reddy, Kirtana</au><au>Ramachandran, Balaji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A reproducible and affordable method of conducting luciferase assay</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2024-09-01</date><risdate>2024</risdate><volume>692</volume><spage>115558</spage><pages>115558-</pages><artnum>115558</artnum><issn>0003-2697</issn><issn>1096-0309</issn><eissn>1096-0309</eissn><abstract>Commercially available glow luciferase assay kits are widely popular and convenient to use. However, concerning high-throughput screening, commercial kits are limited by huge running costs. As an alternative to commercial luciferase assay kits, this study presents a cost-effective and efficient methodology of performing a simple and rapid laboratory flash luciferase assay. The proposed luciferase assay method has a versatile use ranging from screening lysates in a microplate reader for quantitative assay as well as screening live cells qualitatively or quantitatively under an imaging system.
[Display omitted]
•This study demonstrates a reliable, and reproducible alternative to commercial luciferase assay kits.•Can be used for high-throughput luciferase activity screening due to its cost-effectiveness.•Proposes a composition and combination of luciferase assay buffers and lysis buffers for efficient screening.•This assay can be applied for quantitative cell-lysate screening for luciferase activity in microplate readers.•Can be applied to qualitative or quantitative screening of luciferase assay in live –cells using an imaging system.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>38735426</pmid><doi>10.1016/j.ab.2024.115558</doi><orcidid>https://orcid.org/0000-0003-1882-9717</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 2024-09, Vol.692, p.115558, Article 115558 |
| issn | 0003-2697 1096-0309 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3054433686 |
| source | Access via ScienceDirect (Elsevier) |
| subjects | Dual-Luciferase assay Firefly luciferase assay buffer Luciferin and coelenterazine Passive lysis buffer Renilla luciferase assay buffer |
| title | A reproducible and affordable method of conducting luciferase assay |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T06%3A28%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20reproducible%20and%20affordable%20method%20of%20conducting%20luciferase%20assay&rft.jtitle=Analytical%20biochemistry&rft.au=Reddy,%20Kirtana&rft.date=2024-09-01&rft.volume=692&rft.spage=115558&rft.pages=115558-&rft.artnum=115558&rft.issn=0003-2697&rft.eissn=1096-0309&rft_id=info:doi/10.1016/j.ab.2024.115558&rft_dat=%3Cproquest_cross%3E3054433686%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3054433686&rft_id=info:pmid/38735426&rft_els_id=S0003269724001027&rfr_iscdi=true |