A reproducible and affordable method of conducting luciferase assay

Commercially available glow luciferase assay kits are widely popular and convenient to use. However, concerning high-throughput screening, commercial kits are limited by huge running costs. As an alternative to commercial luciferase assay kits, this study presents a cost-effective and efficient methodology of performing a simple and rapid laboratory flash luciferase assay. The proposed luciferase assay method has a versatile use ranging from screening lysates in a microplate reader for quantitative assay as well as screening live cells qualitatively or quantitatively under an imaging system. [Display omitted] •This study demonstrates a reliable, and reproducible alternative to commercial luciferase assay kits.•Can be used for high-throughput luciferase activity screening due to its cost-effectiveness.•Proposes a composition and combination of luciferase assay buffers and lysis buffers for efficient screening.•This assay can be applied for quantitative cell-lysate screening for luciferase activity in microplate readers.•Can be applied to qualitative or quantitative screening of luciferase assay in live –cells using an imaging system.