Deposition of collagen III and alterations in basement membrane integrity as candidate prognostic markers in prostate cancer
The extracellular matrix surrounding the tumor undergoes changes in its organization during the metastasis process. The present study aims to quantify total collagen, collagen I (Col I) and collagen III (Col III), analyze the alignment of collagen fibers and assess the basement membrane integrity in...
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| Veröffentlicht in: | Experimental cell research 2024-06, Vol.439 (1), p.114077, Article 114077 |
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| creator | Pinheiro, Laís Capelasso Lucas Pupim, Andreia Carla Eugênio Pereira, Érica Romão Ahrens, Tainah Mendes Mendonça, Ana Carla Francelino, Amanda Letícia Araújo, Eduardo José de Almeida Guembarovski, Alda Fiorina Maria Losi Fuganti, Paulo Emílio Vanzela, André Luís Laforga Colus, Ilce Mara de Syllos Favaron, Phelipe Oliveira Miqueloto, Carlos Alberto Guembarovski, Roberta Losi |
| description | The extracellular matrix surrounding the tumor undergoes changes in its organization during the metastasis process. The present study aims to quantify total collagen, collagen I (Col I) and collagen III (Col III), analyze the alignment of collagen fibers and assess the basement membrane integrity in samples from patients with metastatic and non-metastatic prostate cancer. Tissue samples from 60 patients were classified into groups based on prognostic parameters: better prognosis (n = 20), worse prognosis without metastasis (n = 23) and metastatic (n = 17). Picrosirius red with further analysis under polarizing microscope was used to quantify (with validation using immunohistochemistry) and analyze collagen alignment, and Periodic Acid Schiff staining was used to analyze the basement membrane integrity. The Col I/Col III ratio was found to be higher in the metastatic group than in the groups with better prognosis (p = 0.012) and worse prognosis without metastasis (p = 0.018). Basement membrane integrity constitution in malignant tumor tissue differed from that of adjacent non-tumor tissue (p |
| doi_str_mv | 10.1016/j.yexcr.2024.114077 |
| format | Article |
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•Type III collagen (Col III) had lower deposition in metastatic prostate cancer (PCa) group.•Disruptions in basement membrane integrity were associated with PCa invasive parameters.•Col III and basement membrane are good predictive markers for a worse PCa prognosis.</description><identifier>ISSN: 0014-4827</identifier><identifier>ISSN: 1090-2422</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/j.yexcr.2024.114077</identifier><identifier>PMID: 38735620</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aged ; Basement Membrane - metabolism ; Basement Membrane - pathology ; Biomarkers, Tumor - metabolism ; Col I/ col III ratio ; Collagen I ; Collagen Type I - metabolism ; Collagen Type III - metabolism ; Extracellular matrix ; Extracellular Matrix - metabolism ; Extracellular Matrix - pathology ; Humans ; Male ; Middle Aged ; PAS ; Picrosirius ; Prognosis ; Prostatic Neoplasms - metabolism ; Prostatic Neoplasms - pathology</subject><ispartof>Experimental cell research, 2024-06, Vol.439 (1), p.114077, Article 114077</ispartof><rights>2024 Elsevier Inc.</rights><rights>Copyright © 2024 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c309t-401a64c1ebd9c922153a47aafd9112a8339da31c9d7704fcffce42b7427a0d9a3</cites><orcidid>0000-0002-6735-1791 ; 0000-0002-9769-6715</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.yexcr.2024.114077$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38735620$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pinheiro, Laís Capelasso Lucas</creatorcontrib><creatorcontrib>Pupim, Andreia Carla Eugênio</creatorcontrib><creatorcontrib>Pereira, Érica Romão</creatorcontrib><creatorcontrib>Ahrens, Tainah Mendes</creatorcontrib><creatorcontrib>Mendonça, Ana Carla</creatorcontrib><creatorcontrib>Francelino, Amanda Letícia</creatorcontrib><creatorcontrib>Araújo, Eduardo José de Almeida</creatorcontrib><creatorcontrib>Guembarovski, Alda Fiorina Maria Losi</creatorcontrib><creatorcontrib>Fuganti, Paulo Emílio</creatorcontrib><creatorcontrib>Vanzela, André Luís Laforga</creatorcontrib><creatorcontrib>Colus, Ilce Mara de Syllos</creatorcontrib><creatorcontrib>Favaron, Phelipe Oliveira</creatorcontrib><creatorcontrib>Miqueloto, Carlos Alberto</creatorcontrib><creatorcontrib>Guembarovski, Roberta Losi</creatorcontrib><title>Deposition of collagen III and alterations in basement membrane integrity as candidate prognostic markers in prostate cancer</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>The extracellular matrix surrounding the tumor undergoes changes in its organization during the metastasis process. The present study aims to quantify total collagen, collagen I (Col I) and collagen III (Col III), analyze the alignment of collagen fibers and assess the basement membrane integrity in samples from patients with metastatic and non-metastatic prostate cancer. Tissue samples from 60 patients were classified into groups based on prognostic parameters: better prognosis (n = 20), worse prognosis without metastasis (n = 23) and metastatic (n = 17). Picrosirius red with further analysis under polarizing microscope was used to quantify (with validation using immunohistochemistry) and analyze collagen alignment, and Periodic Acid Schiff staining was used to analyze the basement membrane integrity. The Col I/Col III ratio was found to be higher in the metastatic group than in the groups with better prognosis (p = 0.012) and worse prognosis without metastasis (p = 0.018). Basement membrane integrity constitution in malignant tumor tissue differed from that of adjacent non-tumor tissue (p < 0.001). Moreover, the worsening in the tumor tissue integrity was positively correlated with worse prognostic parameters. All in all, absence of Col III and basement membrane integrity might be indicators of poor prognosis in prostate cancer.
[Display omitted]
•Type III collagen (Col III) had lower deposition in metastatic prostate cancer (PCa) group.•Disruptions in basement membrane integrity were associated with PCa invasive parameters.•Col III and basement membrane are good predictive markers for a worse PCa prognosis.</description><subject>Aged</subject><subject>Basement Membrane - metabolism</subject><subject>Basement Membrane - pathology</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Col I/ col III ratio</subject><subject>Collagen I</subject><subject>Collagen Type I - metabolism</subject><subject>Collagen Type III - metabolism</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - metabolism</subject><subject>Extracellular Matrix - pathology</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>PAS</subject><subject>Picrosirius</subject><subject>Prognosis</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Prostatic Neoplasms - pathology</subject><issn>0014-4827</issn><issn>1090-2422</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1rGzEQxUVpqR23f0Eh6NjLOqMPW7uHHkKaD0Mgl_YsZqVZI8e760pyiSF_fOU46TEnwcx7evN-jH0TMBcglheb-YGeXJxLkHouhAZjPrCpgAYqqaX8yKYAQle6lmbCzlLaAEBdi-VnNlG1UYulhCl7_km7MYUcxoGPHXfjdotrGvhqteI4eI7bTBGP68TDwFtM1NOQeU99G3GgMsy0jiEfOCbuiiV4zMR3cVwPY8rB8R7jI8UXe5mmfFwXoaP4hX3qcJvo6-s7Y79vrn9d3VX3D7erq8v7yilocqVB4FI7Qa1vXCOlWCjUBrHzjRASa6Uaj0q4xhsDunNd50jL1mhpEHyDasa-n_4t-X_2lLLtQ3JUqg407pNVsNBaKRBQpOokdeXUFKmzuxhKg4MVYI_Y7ca-YLdH7PaEvbjOXwP2bU_-v-eNcxH8OAmo1PwbKNrkAhUGPkRy2foxvBvwD3BHluo</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Pinheiro, Laís Capelasso Lucas</creator><creator>Pupim, Andreia Carla Eugênio</creator><creator>Pereira, Érica Romão</creator><creator>Ahrens, Tainah Mendes</creator><creator>Mendonça, Ana Carla</creator><creator>Francelino, Amanda Letícia</creator><creator>Araújo, Eduardo José de Almeida</creator><creator>Guembarovski, Alda Fiorina Maria Losi</creator><creator>Fuganti, Paulo Emílio</creator><creator>Vanzela, André Luís Laforga</creator><creator>Colus, Ilce Mara de Syllos</creator><creator>Favaron, Phelipe Oliveira</creator><creator>Miqueloto, Carlos Alberto</creator><creator>Guembarovski, Roberta Losi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6735-1791</orcidid><orcidid>https://orcid.org/0000-0002-9769-6715</orcidid></search><sort><creationdate>20240601</creationdate><title>Deposition of collagen III and alterations in basement membrane integrity as candidate prognostic markers in prostate cancer</title><author>Pinheiro, Laís Capelasso Lucas ; Pupim, Andreia Carla Eugênio ; Pereira, Érica Romão ; Ahrens, Tainah Mendes ; Mendonça, Ana Carla ; Francelino, Amanda Letícia ; Araújo, Eduardo José de Almeida ; Guembarovski, Alda Fiorina Maria Losi ; Fuganti, Paulo Emílio ; Vanzela, André Luís Laforga ; Colus, Ilce Mara de Syllos ; Favaron, Phelipe Oliveira ; Miqueloto, Carlos Alberto ; Guembarovski, Roberta Losi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c309t-401a64c1ebd9c922153a47aafd9112a8339da31c9d7704fcffce42b7427a0d9a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aged</topic><topic>Basement Membrane - metabolism</topic><topic>Basement Membrane - pathology</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Col I/ col III ratio</topic><topic>Collagen I</topic><topic>Collagen Type I - metabolism</topic><topic>Collagen Type III - metabolism</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - metabolism</topic><topic>Extracellular Matrix - pathology</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>PAS</topic><topic>Picrosirius</topic><topic>Prognosis</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Prostatic Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pinheiro, Laís Capelasso Lucas</creatorcontrib><creatorcontrib>Pupim, Andreia Carla Eugênio</creatorcontrib><creatorcontrib>Pereira, Érica Romão</creatorcontrib><creatorcontrib>Ahrens, Tainah Mendes</creatorcontrib><creatorcontrib>Mendonça, Ana Carla</creatorcontrib><creatorcontrib>Francelino, Amanda Letícia</creatorcontrib><creatorcontrib>Araújo, Eduardo José de Almeida</creatorcontrib><creatorcontrib>Guembarovski, Alda Fiorina Maria Losi</creatorcontrib><creatorcontrib>Fuganti, Paulo Emílio</creatorcontrib><creatorcontrib>Vanzela, André Luís Laforga</creatorcontrib><creatorcontrib>Colus, Ilce Mara de Syllos</creatorcontrib><creatorcontrib>Favaron, Phelipe Oliveira</creatorcontrib><creatorcontrib>Miqueloto, Carlos Alberto</creatorcontrib><creatorcontrib>Guembarovski, Roberta Losi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pinheiro, Laís Capelasso Lucas</au><au>Pupim, Andreia Carla Eugênio</au><au>Pereira, Érica Romão</au><au>Ahrens, Tainah Mendes</au><au>Mendonça, Ana Carla</au><au>Francelino, Amanda Letícia</au><au>Araújo, Eduardo José de Almeida</au><au>Guembarovski, Alda Fiorina Maria Losi</au><au>Fuganti, Paulo Emílio</au><au>Vanzela, André Luís Laforga</au><au>Colus, Ilce Mara de Syllos</au><au>Favaron, Phelipe Oliveira</au><au>Miqueloto, Carlos Alberto</au><au>Guembarovski, Roberta Losi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deposition of collagen III and alterations in basement membrane integrity as candidate prognostic markers in prostate cancer</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>2024-06-01</date><risdate>2024</risdate><volume>439</volume><issue>1</issue><spage>114077</spage><pages>114077-</pages><artnum>114077</artnum><issn>0014-4827</issn><issn>1090-2422</issn><eissn>1090-2422</eissn><abstract>The extracellular matrix surrounding the tumor undergoes changes in its organization during the metastasis process. The present study aims to quantify total collagen, collagen I (Col I) and collagen III (Col III), analyze the alignment of collagen fibers and assess the basement membrane integrity in samples from patients with metastatic and non-metastatic prostate cancer. Tissue samples from 60 patients were classified into groups based on prognostic parameters: better prognosis (n = 20), worse prognosis without metastasis (n = 23) and metastatic (n = 17). Picrosirius red with further analysis under polarizing microscope was used to quantify (with validation using immunohistochemistry) and analyze collagen alignment, and Periodic Acid Schiff staining was used to analyze the basement membrane integrity. The Col I/Col III ratio was found to be higher in the metastatic group than in the groups with better prognosis (p = 0.012) and worse prognosis without metastasis (p = 0.018). Basement membrane integrity constitution in malignant tumor tissue differed from that of adjacent non-tumor tissue (p < 0.001). Moreover, the worsening in the tumor tissue integrity was positively correlated with worse prognostic parameters. All in all, absence of Col III and basement membrane integrity might be indicators of poor prognosis in prostate cancer.
[Display omitted]
•Type III collagen (Col III) had lower deposition in metastatic prostate cancer (PCa) group.•Disruptions in basement membrane integrity were associated with PCa invasive parameters.•Col III and basement membrane are good predictive markers for a worse PCa prognosis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>38735620</pmid><doi>10.1016/j.yexcr.2024.114077</doi><orcidid>https://orcid.org/0000-0002-6735-1791</orcidid><orcidid>https://orcid.org/0000-0002-9769-6715</orcidid></addata></record> |
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| subjects | Aged Basement Membrane - metabolism Basement Membrane - pathology Biomarkers, Tumor - metabolism Col I/ col III ratio Collagen I Collagen Type I - metabolism Collagen Type III - metabolism Extracellular matrix Extracellular Matrix - metabolism Extracellular Matrix - pathology Humans Male Middle Aged PAS Picrosirius Prognosis Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology |
| title | Deposition of collagen III and alterations in basement membrane integrity as candidate prognostic markers in prostate cancer |
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