An in vivo appraisal of Punica granatum peel extract’s ultrastructural effect on cystic echinococcosis in mice
In the past decade, interest has significantly increased regarding the medicinal and nutritional benefits of pomegranate (Punica granatum) peel. This study examined the effects of using pomegranate peel extract (PGE) alone and in combination with albendazole (ABZ) on ultrastructural and immunologica...
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| Veröffentlicht in: | Journal of helminthology 2024-05, Vol.98, p.e40-e40, Article e40 |
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| creator | Nassef, Nashaat E. Shendi, Sawsan S. Saad, Abdel-Gawad E. Harba, Nancy M. Beshay, Engy V.N. Mohamed, Asmaa Shams El-Dein Gouda, Marwa A. |
| description | In the past decade, interest has significantly increased regarding the medicinal and nutritional benefits of pomegranate (Punica granatum) peel. This study examined the effects of using pomegranate peel extract (PGE) alone and in combination with albendazole (ABZ) on ultrastructural and immunological changes in cystic echinococcosis in laboratory-infected mice. Results revealed that the smallest hydatid cyst size and weight (0.48 ± 0.47mm, 0.17 ± 0.18 gm) with the highest drug efficacy (56.2%) was detected in the PGE + ABZ group, which also exhibited marked histopathological improvement. Ultrastructural changes recorded by transmission electron microscopy including fragmentation of the nucleus, glycogen depletion, and multiple lysosomes in vacuolated cytoplasm were more often observed in PGE + ABZ group. IFN-γ levels were significantly increased in the group treated with ABZ, with a notable reduction following PGE treatment, whether administered alone or in combination with ABZ. Thus, PGE enhanced the therapeutic efficiency of ABZ, with improvement in histopathological and ultrastructural changes. |
| doi_str_mv | 10.1017/S0022149X24000300 |
| format | Article |
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This study examined the effects of using pomegranate peel extract (PGE) alone and in combination with albendazole (ABZ) on ultrastructural and immunological changes in cystic echinococcosis in laboratory-infected mice. Results revealed that the smallest hydatid cyst size and weight (0.48 ± 0.47mm, 0.17 ± 0.18 gm) with the highest drug efficacy (56.2%) was detected in the PGE + ABZ group, which also exhibited marked histopathological improvement. Ultrastructural changes recorded by transmission electron microscopy including fragmentation of the nucleus, glycogen depletion, and multiple lysosomes in vacuolated cytoplasm were more often observed in PGE + ABZ group. IFN-γ levels were significantly increased in the group treated with ABZ, with a notable reduction following PGE treatment, whether administered alone or in combination with ABZ. Thus, PGE enhanced the therapeutic efficiency of ABZ, with improvement in histopathological and ultrastructural changes.</description><identifier>ISSN: 0022-149X</identifier><identifier>EISSN: 1475-2697</identifier><identifier>DOI: 10.1017/S0022149X24000300</identifier><identifier>PMID: 38738533</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Albendazole ; Albendazole - administration & dosage ; Albendazole - pharmacology ; Animals ; Anthelmintics - administration & dosage ; Anthelmintics - pharmacology ; Cysts ; Cytoplasm ; Disease Models, Animal ; Drug dosages ; Drug efficacy ; Echinococcosis ; Echinococcosis - drug therapy ; Echinococcosis - parasitology ; Electron microscopy ; Female ; Fruits ; Glycogen ; Glycogens ; Herbal medicine ; Histopathology ; Immunology ; Infections ; Interferon-gamma - blood ; Laboratory animals ; Liver ; Lysosomes ; Male ; Mice ; Microscopy, Electron, Transmission ; Mortality ; Parasitic diseases ; Plant extracts ; Plant Extracts - administration & dosage ; Plant Extracts - pharmacology ; Pomegranate - chemistry ; Punica granatum ; Research Paper ; Rodents ; Serology ; Transmission electron microscopy ; γ-Interferon</subject><ispartof>Journal of helminthology, 2024-05, Vol.98, p.e40-e40, Article e40</ispartof><rights>The Author(s), 2024. Published by Cambridge University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c325t-478741c76c6ef1866f8ca74e55399ee8ae1aa52686cd26fb1e6174efdde1e6ae3</cites><orcidid>0000-0003-1723-6792</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0022149X24000300/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27924,27925,55628</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38738533$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nassef, Nashaat E.</creatorcontrib><creatorcontrib>Shendi, Sawsan S.</creatorcontrib><creatorcontrib>Saad, Abdel-Gawad E.</creatorcontrib><creatorcontrib>Harba, Nancy M.</creatorcontrib><creatorcontrib>Beshay, Engy V.N.</creatorcontrib><creatorcontrib>Mohamed, Asmaa Shams El-Dein</creatorcontrib><creatorcontrib>Gouda, Marwa A.</creatorcontrib><title>An in vivo appraisal of Punica granatum peel extract’s ultrastructural effect on cystic echinococcosis in mice</title><title>Journal of helminthology</title><addtitle>J. Helminthol</addtitle><description>In the past decade, interest has significantly increased regarding the medicinal and nutritional benefits of pomegranate (Punica granatum) peel. This study examined the effects of using pomegranate peel extract (PGE) alone and in combination with albendazole (ABZ) on ultrastructural and immunological changes in cystic echinococcosis in laboratory-infected mice. Results revealed that the smallest hydatid cyst size and weight (0.48 ± 0.47mm, 0.17 ± 0.18 gm) with the highest drug efficacy (56.2%) was detected in the PGE + ABZ group, which also exhibited marked histopathological improvement. Ultrastructural changes recorded by transmission electron microscopy including fragmentation of the nucleus, glycogen depletion, and multiple lysosomes in vacuolated cytoplasm were more often observed in PGE + ABZ group. IFN-γ levels were significantly increased in the group treated with ABZ, with a notable reduction following PGE treatment, whether administered alone or in combination with ABZ. Thus, PGE enhanced the therapeutic efficiency of ABZ, with improvement in histopathological and ultrastructural changes.</description><subject>Albendazole</subject><subject>Albendazole - administration & dosage</subject><subject>Albendazole - pharmacology</subject><subject>Animals</subject><subject>Anthelmintics - administration & dosage</subject><subject>Anthelmintics - pharmacology</subject><subject>Cysts</subject><subject>Cytoplasm</subject><subject>Disease Models, Animal</subject><subject>Drug dosages</subject><subject>Drug efficacy</subject><subject>Echinococcosis</subject><subject>Echinococcosis - drug therapy</subject><subject>Echinococcosis - parasitology</subject><subject>Electron microscopy</subject><subject>Female</subject><subject>Fruits</subject><subject>Glycogen</subject><subject>Glycogens</subject><subject>Herbal medicine</subject><subject>Histopathology</subject><subject>Immunology</subject><subject>Infections</subject><subject>Interferon-gamma - blood</subject><subject>Laboratory animals</subject><subject>Liver</subject><subject>Lysosomes</subject><subject>Male</subject><subject>Mice</subject><subject>Microscopy, Electron, Transmission</subject><subject>Mortality</subject><subject>Parasitic diseases</subject><subject>Plant extracts</subject><subject>Plant Extracts - administration & dosage</subject><subject>Plant Extracts - pharmacology</subject><subject>Pomegranate - chemistry</subject><subject>Punica granatum</subject><subject>Research Paper</subject><subject>Rodents</subject><subject>Serology</subject><subject>Transmission electron microscopy</subject><subject>γ-Interferon</subject><issn>0022-149X</issn><issn>1475-2697</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1qFUEQhRtRzDX6AG6kwY2b0f6fmWUI0QgBBRXcDX1rqmOHmemxf4LZ-Rq-nk-SHnJVUFxVwfnOqaKKkKecveSMt68-MCYEV_1noRhjkrF7ZMdVqxth-vY-2W1ys-lH5FFKVxvDhX5IjmTXyk5LuSPryUL9Qq_9daB2XaP1yU40OPq-LB4svYx2sbnMdEWcKH7L0UL--f1HomWqfcqxQC6xetA5hEzDQuEmZQ8U4YtfAgSAkHzapswe8DF54OyU8MmhHpNPr88-np43F-_evD09uWhACp0b1Xat4tAaMOh4Z4zrwLYKtZZ9j9hZ5NZqYToDozBuz9HwKrtxxNpalMfkxV3uGsPXgikPs0-A02QXDCUNkmmlpFBCVfT5X-hVKHGp222U7IXu-65S_I6CGFKK6IY1-tnGm4GzYXvH8M87qufZIbnsZxx_O37dvwLyEGrnffTjJf6Z_f_YW_sslsM</recordid><startdate>20240513</startdate><enddate>20240513</enddate><creator>Nassef, Nashaat E.</creator><creator>Shendi, Sawsan S.</creator><creator>Saad, Abdel-Gawad E.</creator><creator>Harba, Nancy M.</creator><creator>Beshay, Engy V.N.</creator><creator>Mohamed, Asmaa Shams El-Dein</creator><creator>Gouda, Marwa A.</creator><general>Cambridge University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7SN</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>K9.</scope><scope>L.G</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1723-6792</orcidid></search><sort><creationdate>20240513</creationdate><title>An in vivo appraisal of Punica granatum peel extract’s ultrastructural effect on cystic echinococcosis in mice</title><author>Nassef, Nashaat E. ; Shendi, Sawsan S. ; Saad, Abdel-Gawad E. ; Harba, Nancy M. ; Beshay, Engy V.N. ; Mohamed, Asmaa Shams El-Dein ; Gouda, Marwa A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c325t-478741c76c6ef1866f8ca74e55399ee8ae1aa52686cd26fb1e6174efdde1e6ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Albendazole</topic><topic>Albendazole - administration & dosage</topic><topic>Albendazole - pharmacology</topic><topic>Animals</topic><topic>Anthelmintics - administration & dosage</topic><topic>Anthelmintics - pharmacology</topic><topic>Cysts</topic><topic>Cytoplasm</topic><topic>Disease Models, Animal</topic><topic>Drug dosages</topic><topic>Drug efficacy</topic><topic>Echinococcosis</topic><topic>Echinococcosis - drug therapy</topic><topic>Echinococcosis - parasitology</topic><topic>Electron microscopy</topic><topic>Female</topic><topic>Fruits</topic><topic>Glycogen</topic><topic>Glycogens</topic><topic>Herbal medicine</topic><topic>Histopathology</topic><topic>Immunology</topic><topic>Infections</topic><topic>Interferon-gamma - blood</topic><topic>Laboratory animals</topic><topic>Liver</topic><topic>Lysosomes</topic><topic>Male</topic><topic>Mice</topic><topic>Microscopy, Electron, Transmission</topic><topic>Mortality</topic><topic>Parasitic diseases</topic><topic>Plant extracts</topic><topic>Plant Extracts - administration & dosage</topic><topic>Plant Extracts - pharmacology</topic><topic>Pomegranate - chemistry</topic><topic>Punica granatum</topic><topic>Research Paper</topic><topic>Rodents</topic><topic>Serology</topic><topic>Transmission electron microscopy</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nassef, Nashaat E.</creatorcontrib><creatorcontrib>Shendi, Sawsan S.</creatorcontrib><creatorcontrib>Saad, Abdel-Gawad E.</creatorcontrib><creatorcontrib>Harba, Nancy M.</creatorcontrib><creatorcontrib>Beshay, Engy V.N.</creatorcontrib><creatorcontrib>Mohamed, Asmaa Shams El-Dein</creatorcontrib><creatorcontrib>Gouda, Marwa A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of helminthology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nassef, Nashaat E.</au><au>Shendi, Sawsan S.</au><au>Saad, Abdel-Gawad E.</au><au>Harba, Nancy M.</au><au>Beshay, Engy V.N.</au><au>Mohamed, Asmaa Shams El-Dein</au><au>Gouda, Marwa A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An in vivo appraisal of Punica granatum peel extract’s ultrastructural effect on cystic echinococcosis in mice</atitle><jtitle>Journal of helminthology</jtitle><addtitle>J. Helminthol</addtitle><date>2024-05-13</date><risdate>2024</risdate><volume>98</volume><spage>e40</spage><epage>e40</epage><pages>e40-e40</pages><artnum>e40</artnum><issn>0022-149X</issn><eissn>1475-2697</eissn><abstract>In the past decade, interest has significantly increased regarding the medicinal and nutritional benefits of pomegranate (Punica granatum) peel. This study examined the effects of using pomegranate peel extract (PGE) alone and in combination with albendazole (ABZ) on ultrastructural and immunological changes in cystic echinococcosis in laboratory-infected mice. Results revealed that the smallest hydatid cyst size and weight (0.48 ± 0.47mm, 0.17 ± 0.18 gm) with the highest drug efficacy (56.2%) was detected in the PGE + ABZ group, which also exhibited marked histopathological improvement. Ultrastructural changes recorded by transmission electron microscopy including fragmentation of the nucleus, glycogen depletion, and multiple lysosomes in vacuolated cytoplasm were more often observed in PGE + ABZ group. IFN-γ levels were significantly increased in the group treated with ABZ, with a notable reduction following PGE treatment, whether administered alone or in combination with ABZ. Thus, PGE enhanced the therapeutic efficiency of ABZ, with improvement in histopathological and ultrastructural changes.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>38738533</pmid><doi>10.1017/S0022149X24000300</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-1723-6792</orcidid></addata></record> |
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| subjects | Albendazole Albendazole - administration & dosage Albendazole - pharmacology Animals Anthelmintics - administration & dosage Anthelmintics - pharmacology Cysts Cytoplasm Disease Models, Animal Drug dosages Drug efficacy Echinococcosis Echinococcosis - drug therapy Echinococcosis - parasitology Electron microscopy Female Fruits Glycogen Glycogens Herbal medicine Histopathology Immunology Infections Interferon-gamma - blood Laboratory animals Liver Lysosomes Male Mice Microscopy, Electron, Transmission Mortality Parasitic diseases Plant extracts Plant Extracts - administration & dosage Plant Extracts - pharmacology Pomegranate - chemistry Punica granatum Research Paper Rodents Serology Transmission electron microscopy γ-Interferon |
| title | An in vivo appraisal of Punica granatum peel extract’s ultrastructural effect on cystic echinococcosis in mice |
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