Wound healing potential of a formula based on Populus nigra L. flower buds extract with anti-inflammatory activity
Wound healing is a complex and dysnamic process supported by a myriad of cellular events that are tightly coordinated to repair efficiently damaged tissue. Populus nigra L. (Salicaceae) flower buds are traditionally used in the treatment of dermatitis, upper respiratory tract infections, rheumatism...
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| Veröffentlicht in: | Journal of ethnopharmacology 2024-09, Vol.331, p.118319-118319, Article 118319 |
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| creator | Atia, Amina Atmani-Kilani, Dina Atmani, Djebbar Ayouni, Karima Belkhir, Sarra Benloukil, Malika Saidene, Naima Moulaoui, Kenza Kasmi, Souad Medjahed, Zineb Boussebaa, Walid Atmani, Djamila |
| description | Wound healing is a complex and dysnamic process supported by a myriad of cellular events that are tightly coordinated to repair efficiently damaged tissue. Populus nigra L. (Salicaceae) flower buds are traditionally used in the treatment of dermatitis, upper respiratory tract infections, rheumatism and wounds.
The aim of this study was to assess the wound healing potential of black poplar ointment containing 10 or 20 % of Populus nigra ethanolic flower buds extract using the excision model in rats.
Two ointments (10 and 20 %) were prepared from Populus nigra flower buds ethanolic extract and topically applied on the area of excised skin of the rats for either 14 or 20 days. Morphological, macroscopic, histological and biochemical parameters were evaluated.
The results showed that the extract contained high amounts of total phenols (89.5 ± 7.7 mg caffeic acid equivalent/g of extract) and hydrolysable tannins (142.05 ± 2.55 mg tannic acid equivalent/g of extract), in correlation with strong DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity and beta-carotene bleaching with values of 96.31 ± 3.42 and 85.27 ± 1.79 %, respectively. Anti-inflammatory potential was illustrated by lipoxygenase and cyclooxygenase inhibition (52.80 ± 0.2 and 53.88 ± 2.55 %, respectively). Treatment with Populus nigra ointment (10 and 20 %) promoted wound contraction of 97.37 ± 1.19 and 97.28 ± 0.91 %, respectively. The antioxidant marker enzymes, catalase (0.10 ± 0.001; 0.08 ± 0.003 U/mg protein) and superoxide dismutase (363.34 ± 24.37; 317.82 ± 53.83 U/mg protein) activities in the granulation tissues were upgraded with respective treatments of 10 or 20 % ointment. Concurrently, the myeloperoxidase activity (2.21 ± 1.01; 2.13 ± 0.75 U/mg protein) was repressed, indicating anti-inflammatory potential, when compared to untreated, standard and excipient groups. Moreover, a significant increase in respective levels of hydroxyproline (p |
| doi_str_mv | 10.1016/j.jep.2024.118319 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3053974249</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378874124006184</els_id><sourcerecordid>3053974249</sourcerecordid><originalsourceid>FETCH-LOGICAL-c305t-1865e3de0346f01583e4b6de5b52ea99e8bd623652c45ea9c823ea025fbf202b3</originalsourceid><addsrcrecordid>eNp9kE1v1DAQhi1ERZfCD-CCfOSS4I84ccQJVeVDWgkOrThajj1uvXLiYDst--_ragtHTiPNPPOO5kHoHSUtJbT_eGgPsLaMsK6lVHI6vkA7KgfWDGLgL9GO8EE2cujoOXqd84EQMtCOvELnvEKj4HKH0q-4LRbfgQ5-ucVrLLAUrwOODmvsYpq3oPGkM1gcF_wzrlvYMl78bdJ432IX4gMkPG02Y_hTkjYFP_hyh3WNafzigp5nXWI64jry974c36Azp0OGt8_1At18ubq-_Nbsf3z9fvl53xhORGmo7AVwC4R3vSNUSA7d1FsQk2CgxxHkZHvGe8FMJ2rDSMZBEybc5KqSiV-gD6fcNcXfG-SiZp8NhKAXiFtW9Qofh451Y0XpCTUp5pzAqTX5WaejokQ9qVYHVVWrJ9XqpLruvH-O36YZ7L-Nv24r8OkEQH3y3kNS2XhYDFifwBRlo_9P_CMLlo_-</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3053974249</pqid></control><display><type>article</type><title>Wound healing potential of a formula based on Populus nigra L. flower buds extract with anti-inflammatory activity</title><source>Elsevier ScienceDirect Journals</source><creator>Atia, Amina ; Atmani-Kilani, Dina ; Atmani, Djebbar ; Ayouni, Karima ; Belkhir, Sarra ; Benloukil, Malika ; Saidene, Naima ; Moulaoui, Kenza ; Kasmi, Souad ; Medjahed, Zineb ; Boussebaa, Walid ; Atmani, Djamila</creator><creatorcontrib>Atia, Amina ; Atmani-Kilani, Dina ; Atmani, Djebbar ; Ayouni, Karima ; Belkhir, Sarra ; Benloukil, Malika ; Saidene, Naima ; Moulaoui, Kenza ; Kasmi, Souad ; Medjahed, Zineb ; Boussebaa, Walid ; Atmani, Djamila</creatorcontrib><description>Wound healing is a complex and dysnamic process supported by a myriad of cellular events that are tightly coordinated to repair efficiently damaged tissue. Populus nigra L. (Salicaceae) flower buds are traditionally used in the treatment of dermatitis, upper respiratory tract infections, rheumatism and wounds.
The aim of this study was to assess the wound healing potential of black poplar ointment containing 10 or 20 % of Populus nigra ethanolic flower buds extract using the excision model in rats.
Two ointments (10 and 20 %) were prepared from Populus nigra flower buds ethanolic extract and topically applied on the area of excised skin of the rats for either 14 or 20 days. Morphological, macroscopic, histological and biochemical parameters were evaluated.
The results showed that the extract contained high amounts of total phenols (89.5 ± 7.7 mg caffeic acid equivalent/g of extract) and hydrolysable tannins (142.05 ± 2.55 mg tannic acid equivalent/g of extract), in correlation with strong DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity and beta-carotene bleaching with values of 96.31 ± 3.42 and 85.27 ± 1.79 %, respectively. Anti-inflammatory potential was illustrated by lipoxygenase and cyclooxygenase inhibition (52.80 ± 0.2 and 53.88 ± 2.55 %, respectively). Treatment with Populus nigra ointment (10 and 20 %) promoted wound contraction of 97.37 ± 1.19 and 97.28 ± 0.91 %, respectively. The antioxidant marker enzymes, catalase (0.10 ± 0.001; 0.08 ± 0.003 U/mg protein) and superoxide dismutase (363.34 ± 24.37; 317.82 ± 53.83 U/mg protein) activities in the granulation tissues were upgraded with respective treatments of 10 or 20 % ointment. Concurrently, the myeloperoxidase activity (2.21 ± 1.01; 2.13 ± 0.75 U/mg protein) was repressed, indicating anti-inflammatory potential, when compared to untreated, standard and excipient groups. Moreover, a significant increase in respective levels of hydroxyproline (p < 0.001) (28.05 ± 1.20; 25.29 ± 1.17 μg/mg tissue) and hexosamine (p < 0.05) (20.18 ± 1.21; 18.95 ± 1.98 μg/mg tissue) was triggered, reflecting a high regeneration of collagen in the scarred tissue. Histological examination of treated skin tissue revealed higher rates of re-epithelialization, lower neutrophils infiltration and re-vascularization in comparison to the control group.
Given that the 10 % ointment was the optimal concentration, our findings offer an efficient drug formula for wound healing.
[Display omitted]
•Populus nigra flower buds are traditionally used for wound healing.•P. nigra ethanolic extract showed potent inhibition of COX-1 and LOX in vitro.•Phenolic compounds participate in different aspects of wound healing.•P. nigra ethanolic extract ointment at 10 % attenuates inflammation in vivo.•Topical application of ointment at 10 % promotes dermal wound healing in rats.</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2024.118319</identifier><identifier>PMID: 38729538</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Collagen ; Cyclooxygenase ; Inflammation ; Populus nigra ; Wound healing</subject><ispartof>Journal of ethnopharmacology, 2024-09, Vol.331, p.118319-118319, Article 118319</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c305t-1865e3de0346f01583e4b6de5b52ea99e8bd623652c45ea9c823ea025fbf202b3</cites><orcidid>0009-0001-2348-6192</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378874124006184$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38729538$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Atia, Amina</creatorcontrib><creatorcontrib>Atmani-Kilani, Dina</creatorcontrib><creatorcontrib>Atmani, Djebbar</creatorcontrib><creatorcontrib>Ayouni, Karima</creatorcontrib><creatorcontrib>Belkhir, Sarra</creatorcontrib><creatorcontrib>Benloukil, Malika</creatorcontrib><creatorcontrib>Saidene, Naima</creatorcontrib><creatorcontrib>Moulaoui, Kenza</creatorcontrib><creatorcontrib>Kasmi, Souad</creatorcontrib><creatorcontrib>Medjahed, Zineb</creatorcontrib><creatorcontrib>Boussebaa, Walid</creatorcontrib><creatorcontrib>Atmani, Djamila</creatorcontrib><title>Wound healing potential of a formula based on Populus nigra L. flower buds extract with anti-inflammatory activity</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Wound healing is a complex and dysnamic process supported by a myriad of cellular events that are tightly coordinated to repair efficiently damaged tissue. Populus nigra L. (Salicaceae) flower buds are traditionally used in the treatment of dermatitis, upper respiratory tract infections, rheumatism and wounds.
The aim of this study was to assess the wound healing potential of black poplar ointment containing 10 or 20 % of Populus nigra ethanolic flower buds extract using the excision model in rats.
Two ointments (10 and 20 %) were prepared from Populus nigra flower buds ethanolic extract and topically applied on the area of excised skin of the rats for either 14 or 20 days. Morphological, macroscopic, histological and biochemical parameters were evaluated.
The results showed that the extract contained high amounts of total phenols (89.5 ± 7.7 mg caffeic acid equivalent/g of extract) and hydrolysable tannins (142.05 ± 2.55 mg tannic acid equivalent/g of extract), in correlation with strong DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity and beta-carotene bleaching with values of 96.31 ± 3.42 and 85.27 ± 1.79 %, respectively. Anti-inflammatory potential was illustrated by lipoxygenase and cyclooxygenase inhibition (52.80 ± 0.2 and 53.88 ± 2.55 %, respectively). Treatment with Populus nigra ointment (10 and 20 %) promoted wound contraction of 97.37 ± 1.19 and 97.28 ± 0.91 %, respectively. The antioxidant marker enzymes, catalase (0.10 ± 0.001; 0.08 ± 0.003 U/mg protein) and superoxide dismutase (363.34 ± 24.37; 317.82 ± 53.83 U/mg protein) activities in the granulation tissues were upgraded with respective treatments of 10 or 20 % ointment. Concurrently, the myeloperoxidase activity (2.21 ± 1.01; 2.13 ± 0.75 U/mg protein) was repressed, indicating anti-inflammatory potential, when compared to untreated, standard and excipient groups. Moreover, a significant increase in respective levels of hydroxyproline (p < 0.001) (28.05 ± 1.20; 25.29 ± 1.17 μg/mg tissue) and hexosamine (p < 0.05) (20.18 ± 1.21; 18.95 ± 1.98 μg/mg tissue) was triggered, reflecting a high regeneration of collagen in the scarred tissue. Histological examination of treated skin tissue revealed higher rates of re-epithelialization, lower neutrophils infiltration and re-vascularization in comparison to the control group.
Given that the 10 % ointment was the optimal concentration, our findings offer an efficient drug formula for wound healing.
[Display omitted]
•Populus nigra flower buds are traditionally used for wound healing.•P. nigra ethanolic extract showed potent inhibition of COX-1 and LOX in vitro.•Phenolic compounds participate in different aspects of wound healing.•P. nigra ethanolic extract ointment at 10 % attenuates inflammation in vivo.•Topical application of ointment at 10 % promotes dermal wound healing in rats.</description><subject>Collagen</subject><subject>Cyclooxygenase</subject><subject>Inflammation</subject><subject>Populus nigra</subject><subject>Wound healing</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kE1v1DAQhi1ERZfCD-CCfOSS4I84ccQJVeVDWgkOrThajj1uvXLiYDst--_ragtHTiPNPPOO5kHoHSUtJbT_eGgPsLaMsK6lVHI6vkA7KgfWDGLgL9GO8EE2cujoOXqd84EQMtCOvELnvEKj4HKH0q-4LRbfgQ5-ucVrLLAUrwOODmvsYpq3oPGkM1gcF_wzrlvYMl78bdJ432IX4gMkPG02Y_hTkjYFP_hyh3WNafzigp5nXWI64jry974c36Azp0OGt8_1At18ubq-_Nbsf3z9fvl53xhORGmo7AVwC4R3vSNUSA7d1FsQk2CgxxHkZHvGe8FMJ2rDSMZBEybc5KqSiV-gD6fcNcXfG-SiZp8NhKAXiFtW9Qofh451Y0XpCTUp5pzAqTX5WaejokQ9qVYHVVWrJ9XqpLruvH-O36YZ7L-Nv24r8OkEQH3y3kNS2XhYDFifwBRlo_9P_CMLlo_-</recordid><startdate>20240915</startdate><enddate>20240915</enddate><creator>Atia, Amina</creator><creator>Atmani-Kilani, Dina</creator><creator>Atmani, Djebbar</creator><creator>Ayouni, Karima</creator><creator>Belkhir, Sarra</creator><creator>Benloukil, Malika</creator><creator>Saidene, Naima</creator><creator>Moulaoui, Kenza</creator><creator>Kasmi, Souad</creator><creator>Medjahed, Zineb</creator><creator>Boussebaa, Walid</creator><creator>Atmani, Djamila</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0001-2348-6192</orcidid></search><sort><creationdate>20240915</creationdate><title>Wound healing potential of a formula based on Populus nigra L. flower buds extract with anti-inflammatory activity</title><author>Atia, Amina ; Atmani-Kilani, Dina ; Atmani, Djebbar ; Ayouni, Karima ; Belkhir, Sarra ; Benloukil, Malika ; Saidene, Naima ; Moulaoui, Kenza ; Kasmi, Souad ; Medjahed, Zineb ; Boussebaa, Walid ; Atmani, Djamila</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-1865e3de0346f01583e4b6de5b52ea99e8bd623652c45ea9c823ea025fbf202b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Collagen</topic><topic>Cyclooxygenase</topic><topic>Inflammation</topic><topic>Populus nigra</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Atia, Amina</creatorcontrib><creatorcontrib>Atmani-Kilani, Dina</creatorcontrib><creatorcontrib>Atmani, Djebbar</creatorcontrib><creatorcontrib>Ayouni, Karima</creatorcontrib><creatorcontrib>Belkhir, Sarra</creatorcontrib><creatorcontrib>Benloukil, Malika</creatorcontrib><creatorcontrib>Saidene, Naima</creatorcontrib><creatorcontrib>Moulaoui, Kenza</creatorcontrib><creatorcontrib>Kasmi, Souad</creatorcontrib><creatorcontrib>Medjahed, Zineb</creatorcontrib><creatorcontrib>Boussebaa, Walid</creatorcontrib><creatorcontrib>Atmani, Djamila</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Atia, Amina</au><au>Atmani-Kilani, Dina</au><au>Atmani, Djebbar</au><au>Ayouni, Karima</au><au>Belkhir, Sarra</au><au>Benloukil, Malika</au><au>Saidene, Naima</au><au>Moulaoui, Kenza</au><au>Kasmi, Souad</au><au>Medjahed, Zineb</au><au>Boussebaa, Walid</au><au>Atmani, Djamila</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wound healing potential of a formula based on Populus nigra L. flower buds extract with anti-inflammatory activity</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2024-09-15</date><risdate>2024</risdate><volume>331</volume><spage>118319</spage><epage>118319</epage><pages>118319-118319</pages><artnum>118319</artnum><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Wound healing is a complex and dysnamic process supported by a myriad of cellular events that are tightly coordinated to repair efficiently damaged tissue. Populus nigra L. (Salicaceae) flower buds are traditionally used in the treatment of dermatitis, upper respiratory tract infections, rheumatism and wounds.
The aim of this study was to assess the wound healing potential of black poplar ointment containing 10 or 20 % of Populus nigra ethanolic flower buds extract using the excision model in rats.
Two ointments (10 and 20 %) were prepared from Populus nigra flower buds ethanolic extract and topically applied on the area of excised skin of the rats for either 14 or 20 days. Morphological, macroscopic, histological and biochemical parameters were evaluated.
The results showed that the extract contained high amounts of total phenols (89.5 ± 7.7 mg caffeic acid equivalent/g of extract) and hydrolysable tannins (142.05 ± 2.55 mg tannic acid equivalent/g of extract), in correlation with strong DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity and beta-carotene bleaching with values of 96.31 ± 3.42 and 85.27 ± 1.79 %, respectively. Anti-inflammatory potential was illustrated by lipoxygenase and cyclooxygenase inhibition (52.80 ± 0.2 and 53.88 ± 2.55 %, respectively). Treatment with Populus nigra ointment (10 and 20 %) promoted wound contraction of 97.37 ± 1.19 and 97.28 ± 0.91 %, respectively. The antioxidant marker enzymes, catalase (0.10 ± 0.001; 0.08 ± 0.003 U/mg protein) and superoxide dismutase (363.34 ± 24.37; 317.82 ± 53.83 U/mg protein) activities in the granulation tissues were upgraded with respective treatments of 10 or 20 % ointment. Concurrently, the myeloperoxidase activity (2.21 ± 1.01; 2.13 ± 0.75 U/mg protein) was repressed, indicating anti-inflammatory potential, when compared to untreated, standard and excipient groups. Moreover, a significant increase in respective levels of hydroxyproline (p < 0.001) (28.05 ± 1.20; 25.29 ± 1.17 μg/mg tissue) and hexosamine (p < 0.05) (20.18 ± 1.21; 18.95 ± 1.98 μg/mg tissue) was triggered, reflecting a high regeneration of collagen in the scarred tissue. Histological examination of treated skin tissue revealed higher rates of re-epithelialization, lower neutrophils infiltration and re-vascularization in comparison to the control group.
Given that the 10 % ointment was the optimal concentration, our findings offer an efficient drug formula for wound healing.
[Display omitted]
•Populus nigra flower buds are traditionally used for wound healing.•P. nigra ethanolic extract showed potent inhibition of COX-1 and LOX in vitro.•Phenolic compounds participate in different aspects of wound healing.•P. nigra ethanolic extract ointment at 10 % attenuates inflammation in vivo.•Topical application of ointment at 10 % promotes dermal wound healing in rats.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>38729538</pmid><doi>10.1016/j.jep.2024.118319</doi><tpages>1</tpages><orcidid>https://orcid.org/0009-0001-2348-6192</orcidid></addata></record> |
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| subjects | Collagen Cyclooxygenase Inflammation Populus nigra Wound healing |
| title | Wound healing potential of a formula based on Populus nigra L. flower buds extract with anti-inflammatory activity |
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