Enhanced Herbicide Metabolism and Target Site Mutation Enabled the Multiple Resistance to Cyhalofop-butyl, Florpyrauxifen-benzyl, and Penoxsulam in Echinochloa crus-galli
This study investigated the multiple herbicide resistance (MHR) mechanism of one Echinochloa crus-galli population that was resistant to florpyrauxifen-benzyl (FPB), cyhalofop-butyl (CHB), and penoxsulam (PEX). This population carried an Ala-122-Asn mutation in the acetolactate synthase (ALS) gene b...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2024-05, Vol.72 (20), p.11405-11414 |
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| creator | Yu, Xiaoyue Sun, Jinqiu Yang, Yongjie Zhang, Jianping Lu, Yongliang Tang, Wei |
| description | This study investigated the multiple herbicide resistance (MHR) mechanism of one Echinochloa crus-galli population that was resistant to florpyrauxifen-benzyl (FPB), cyhalofop-butyl (CHB), and penoxsulam (PEX). This population carried an Ala-122-Asn mutation in the acetolactate synthase (ALS) gene but no mutation in acetyl-CoA carboxylase (ACCase) and transport inhibitor response1 (TIR1) genes. The metabolism rate of PEX was 2-fold higher, and the production of florpyrauxifen-acid and cyhalofop-acid was lower in the resistant population. Malathion and 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) could reverse the resistance, suggesting that cytochrome P450 (CYP450) and glutathione S-transferase (GST) contribute to the enhanced metabolism. According to RNA-seq and qRT-PCR validation, two CYP450 genes (CYP71C42 and CYP71D55), one GST gene (GSTT2), two glycosyltransferase genes (rhamnosyltransferase 1 and IAAGLU), and two ABC transporter genes (ABCG1 and ABCG25) were induced by CHB, FPB, and PEX in the resistant population. This study revealed that the target mutant and enhanced metabolism were involved in the MHR mechanism in E. crus-galli. |
| doi_str_mv | 10.1021/acs.jafc.4c02450 |
| format | Article |
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This population carried an Ala-122-Asn mutation in the acetolactate synthase (ALS) gene but no mutation in acetyl-CoA carboxylase (ACCase) and transport inhibitor response1 (TIR1) genes. The metabolism rate of PEX was 2-fold higher, and the production of florpyrauxifen-acid and cyhalofop-acid was lower in the resistant population. Malathion and 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) could reverse the resistance, suggesting that cytochrome P450 (CYP450) and glutathione S-transferase (GST) contribute to the enhanced metabolism. According to RNA-seq and qRT-PCR validation, two CYP450 genes (CYP71C42 and CYP71D55), one GST gene (GSTT2), two glycosyltransferase genes (rhamnosyltransferase 1 and IAAGLU), and two ABC transporter genes (ABCG1 and ABCG25) were induced by CHB, FPB, and PEX in the resistant population. This study revealed that the target mutant and enhanced metabolism were involved in the MHR mechanism in E. crus-galli.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.4c02450</identifier><identifier>PMID: 38717990</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Acetolactate Synthase - genetics ; Acetolactate Synthase - metabolism ; Acetyl-CoA Carboxylase - genetics ; Acetyl-CoA Carboxylase - metabolism ; Agricultural and Environmental Chemistry ; Butanes ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - metabolism ; Echinochloa - drug effects ; Echinochloa - genetics ; Echinochloa - growth & development ; Echinochloa - metabolism ; Herbicide Resistance - genetics ; Herbicides - metabolism ; Herbicides - pharmacology ; Mutation ; Nitriles ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Weeds - drug effects ; Plant Weeds - genetics ; Plant Weeds - metabolism ; Sulfonamides ; Uridine - analogs & derivatives</subject><ispartof>Journal of agricultural and food chemistry, 2024-05, Vol.72 (20), p.11405-11414</ispartof><rights>2024 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a289t-1bc44c4d9bed9d87deb79973a66289b60c2716de144ecfd2960b6423df9919cf3</cites><orcidid>0000-0002-3241-1125</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.4c02450$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.4c02450$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38717990$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Xiaoyue</creatorcontrib><creatorcontrib>Sun, Jinqiu</creatorcontrib><creatorcontrib>Yang, Yongjie</creatorcontrib><creatorcontrib>Zhang, Jianping</creatorcontrib><creatorcontrib>Lu, Yongliang</creatorcontrib><creatorcontrib>Tang, Wei</creatorcontrib><title>Enhanced Herbicide Metabolism and Target Site Mutation Enabled the Multiple Resistance to Cyhalofop-butyl, Florpyrauxifen-benzyl, and Penoxsulam in Echinochloa crus-galli</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>This study investigated the multiple herbicide resistance (MHR) mechanism of one Echinochloa crus-galli population that was resistant to florpyrauxifen-benzyl (FPB), cyhalofop-butyl (CHB), and penoxsulam (PEX). This population carried an Ala-122-Asn mutation in the acetolactate synthase (ALS) gene but no mutation in acetyl-CoA carboxylase (ACCase) and transport inhibitor response1 (TIR1) genes. The metabolism rate of PEX was 2-fold higher, and the production of florpyrauxifen-acid and cyhalofop-acid was lower in the resistant population. Malathion and 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) could reverse the resistance, suggesting that cytochrome P450 (CYP450) and glutathione S-transferase (GST) contribute to the enhanced metabolism. According to RNA-seq and qRT-PCR validation, two CYP450 genes (CYP71C42 and CYP71D55), one GST gene (GSTT2), two glycosyltransferase genes (rhamnosyltransferase 1 and IAAGLU), and two ABC transporter genes (ABCG1 and ABCG25) were induced by CHB, FPB, and PEX in the resistant population. This study revealed that the target mutant and enhanced metabolism were involved in the MHR mechanism in E. crus-galli.</description><subject>Acetolactate Synthase - genetics</subject><subject>Acetolactate Synthase - metabolism</subject><subject>Acetyl-CoA Carboxylase - genetics</subject><subject>Acetyl-CoA Carboxylase - metabolism</subject><subject>Agricultural and Environmental Chemistry</subject><subject>Butanes</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Echinochloa - drug effects</subject><subject>Echinochloa - genetics</subject><subject>Echinochloa - growth & development</subject><subject>Echinochloa - metabolism</subject><subject>Herbicide Resistance - genetics</subject><subject>Herbicides - metabolism</subject><subject>Herbicides - pharmacology</subject><subject>Mutation</subject><subject>Nitriles</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Weeds - drug effects</subject><subject>Plant Weeds - genetics</subject><subject>Plant Weeds - metabolism</subject><subject>Sulfonamides</subject><subject>Uridine - analogs & derivatives</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctuFDEQRS0EIkNgzwp5ySI92P32Eo0mBCkoCMK65Ud12pHbbvyQMvmkfCVuZmCXlaWqc49Vugi9p2RLSUk_cRm293yU21qSsm7IC7ShTUmKhtL-JdqQzBR909Iz9CaEe0JI33TkNTqr-o52jJENetrbiVsJCl-BF1pqBfgbRC6c0WHG3Cp8y_0dRPxTx7xKkUftLN5bLkxOxWkdmqgXA_gHBB3iqsPR4d1h4saNbilEigdzgS-N88vB8_SgR7CFAPu4jtc_voN1DyEZPmOd5XLS1snJOI6lT6G448bot-jVyE2Ad6f3HP263N_urorrmy9fd5-vC172LBZUyLqWtWICFFN9p0DkU7uKt23ei5bIsqOtAlrXIEdVspaIti4rNTJGmRyrc_Tx6F28-50gxGHWQYIx3IJLYahIU9Gqr5omo-SISu9C8DAOi9cz94eBkmFtaMgNDWtDw6mhHPlwsicxg_of-FdJBi6OwN-oS97mY5_3_QG5JKBe</recordid><startdate>20240522</startdate><enddate>20240522</enddate><creator>Yu, Xiaoyue</creator><creator>Sun, Jinqiu</creator><creator>Yang, Yongjie</creator><creator>Zhang, Jianping</creator><creator>Lu, Yongliang</creator><creator>Tang, Wei</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3241-1125</orcidid></search><sort><creationdate>20240522</creationdate><title>Enhanced Herbicide Metabolism and Target Site Mutation Enabled the Multiple Resistance to Cyhalofop-butyl, Florpyrauxifen-benzyl, and Penoxsulam in Echinochloa crus-galli</title><author>Yu, Xiaoyue ; Sun, Jinqiu ; Yang, Yongjie ; Zhang, Jianping ; Lu, Yongliang ; Tang, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a289t-1bc44c4d9bed9d87deb79973a66289b60c2716de144ecfd2960b6423df9919cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acetolactate Synthase - genetics</topic><topic>Acetolactate Synthase - metabolism</topic><topic>Acetyl-CoA Carboxylase - genetics</topic><topic>Acetyl-CoA Carboxylase - metabolism</topic><topic>Agricultural and Environmental Chemistry</topic><topic>Butanes</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Echinochloa - drug effects</topic><topic>Echinochloa - genetics</topic><topic>Echinochloa - growth & development</topic><topic>Echinochloa - metabolism</topic><topic>Herbicide Resistance - genetics</topic><topic>Herbicides - metabolism</topic><topic>Herbicides - pharmacology</topic><topic>Mutation</topic><topic>Nitriles</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Weeds - drug effects</topic><topic>Plant Weeds - genetics</topic><topic>Plant Weeds - metabolism</topic><topic>Sulfonamides</topic><topic>Uridine - analogs & derivatives</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Xiaoyue</creatorcontrib><creatorcontrib>Sun, Jinqiu</creatorcontrib><creatorcontrib>Yang, Yongjie</creatorcontrib><creatorcontrib>Zhang, Jianping</creatorcontrib><creatorcontrib>Lu, Yongliang</creatorcontrib><creatorcontrib>Tang, Wei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Xiaoyue</au><au>Sun, Jinqiu</au><au>Yang, Yongjie</au><au>Zhang, Jianping</au><au>Lu, Yongliang</au><au>Tang, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced Herbicide Metabolism and Target Site Mutation Enabled the Multiple Resistance to Cyhalofop-butyl, Florpyrauxifen-benzyl, and Penoxsulam in Echinochloa crus-galli</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2024-05-22</date><risdate>2024</risdate><volume>72</volume><issue>20</issue><spage>11405</spage><epage>11414</epage><pages>11405-11414</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>This study investigated the multiple herbicide resistance (MHR) mechanism of one Echinochloa crus-galli population that was resistant to florpyrauxifen-benzyl (FPB), cyhalofop-butyl (CHB), and penoxsulam (PEX). This population carried an Ala-122-Asn mutation in the acetolactate synthase (ALS) gene but no mutation in acetyl-CoA carboxylase (ACCase) and transport inhibitor response1 (TIR1) genes. The metabolism rate of PEX was 2-fold higher, and the production of florpyrauxifen-acid and cyhalofop-acid was lower in the resistant population. Malathion and 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) could reverse the resistance, suggesting that cytochrome P450 (CYP450) and glutathione S-transferase (GST) contribute to the enhanced metabolism. According to RNA-seq and qRT-PCR validation, two CYP450 genes (CYP71C42 and CYP71D55), one GST gene (GSTT2), two glycosyltransferase genes (rhamnosyltransferase 1 and IAAGLU), and two ABC transporter genes (ABCG1 and ABCG25) were induced by CHB, FPB, and PEX in the resistant population. This study revealed that the target mutant and enhanced metabolism were involved in the MHR mechanism in E. crus-galli.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>38717990</pmid><doi>10.1021/acs.jafc.4c02450</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-3241-1125</orcidid></addata></record> |
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| language | eng |
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| source | ACS Publications; MEDLINE |
| subjects | Acetolactate Synthase - genetics Acetolactate Synthase - metabolism Acetyl-CoA Carboxylase - genetics Acetyl-CoA Carboxylase - metabolism Agricultural and Environmental Chemistry Butanes Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Echinochloa - drug effects Echinochloa - genetics Echinochloa - growth & development Echinochloa - metabolism Herbicide Resistance - genetics Herbicides - metabolism Herbicides - pharmacology Mutation Nitriles Plant Proteins - genetics Plant Proteins - metabolism Plant Weeds - drug effects Plant Weeds - genetics Plant Weeds - metabolism Sulfonamides Uridine - analogs & derivatives |
| title | Enhanced Herbicide Metabolism and Target Site Mutation Enabled the Multiple Resistance to Cyhalofop-butyl, Florpyrauxifen-benzyl, and Penoxsulam in Echinochloa crus-galli |
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