The tick Ixodes scapularis has five different GPCRs specifically activated by ACP (adipokinetic hormone/corazonin-related peptide)

Insects have about 50 neuropeptide genes and about 70 genes, coding for neuropeptide G protein-coupled receptors (GPCRs). An important, but small family of evolutionarily related insect neuropeptides consists of adipokinetic hormone (AKH), corazonin, and AKH/corazonin-related peptide (ACP). Normally...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biochemical and biophysical research communications 2024-07, Vol.717, p.149992, Article 149992
Hauptverfasser: Hauser, Frank, Stebegg, Marisa, Al-Ribaty, Tara, Petersen, Lea B., Møller, Mads, Drag, Markus H., Sigurdsson, Haraldur H., Vilhelm, Martin J., Thygesen, Gedske, Grimmelikhuijzen, Cornelis J.P.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page 149992
container_title Biochemical and biophysical research communications
container_volume 717
creator Hauser, Frank
Stebegg, Marisa
Al-Ribaty, Tara
Petersen, Lea B.
Møller, Mads
Drag, Markus H.
Sigurdsson, Haraldur H.
Vilhelm, Martin J.
Thygesen, Gedske
Grimmelikhuijzen, Cornelis J.P.
description Insects have about 50 neuropeptide genes and about 70 genes, coding for neuropeptide G protein-coupled receptors (GPCRs). An important, but small family of evolutionarily related insect neuropeptides consists of adipokinetic hormone (AKH), corazonin, and AKH/corazonin-related peptide (ACP). Normally, insects have one specific GPCR for each of these neuropeptides. The tick Ixodes scapularis is not an insect, but belongs to the subphylum Chelicerata, which comprises ticks, scorpions, mites, spiders, and horseshoe crabs. Many of the neuropeptides and neuropeptide GPCRs occurring in insects, also occur in chelicerates, illustrating that insects and chelicerates are evolutionarily closely related. The tick I. scapularis is an ectoparasite and health risk for humans, because it infects its human host with dangerous pathogens during a blood meal. Understanding the biology of ticks will help researchers to prevent tick-borne diseases. By annotating the I. scapularis genome sequence, we previously found that ticks contain as many as five genes, coding for presumed ACP receptors. In the current paper, we cloned these receptors and expressed each of them in Chinese Hamster Ovary (CHO) cells. Each expressed receptor was activated by nanomolar concentrations of ACP, demonstrating that all five receptors were functional ACP receptors. Phylogenetic tree analyses showed that the cloned tick ACP receptors were mostly related to insect ACP receptors and, next, to insect AKH receptors, suggesting that ACP receptor genes and AKH receptor genes originated by gene duplications from a common ancestor. Similar duplications have probably occurred for the ligand genes, during a process of ligand/receptor co-evolution. Interestingly, chelicerates, in contrast to all other arthropods, do not have AKH or AKH receptor genes. Therefore, the ancestor of chelicerates might have lost AKH and AKH receptor genes and functionally replaced them by ACP and ACP receptor genes. For the small family of AKH, ACP, and corazonin receptors and their ligands, gene losses and gene gains occur frequently between the various ecdysozoan clades. Tardigrades, for example, which are well known for their survival in extreme environments, have as many as ten corazonin receptor genes and six corazonin peptide genes, while insects only have one of each, or none. [Display omitted] •The tick Ixodes scapularis is a vector for serious human diseases, such as Lyme.•G protein-coupled receptors (GPCRs) play a central ro
doi_str_mv 10.1016/j.bbrc.2024.149992
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3052594359</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X2400528X</els_id><sourcerecordid>3052594359</sourcerecordid><originalsourceid>FETCH-LOGICAL-c351t-fcb1fdc967c012d3af73baa284059f73d961c0180b5a2b2527f661b4305cf9853</originalsourceid><addsrcrecordid>eNp9kE1vEzEQhi0EoqHwBzggH8thU4_3I7HEpYqgVKpEhYrEzfLHWJl0s17sTdRw5JfjkMKR04w0z_tK8zD2FsQcBHSXm7m1yc2lkM0cGqWUfMZmIJSoJIjmOZsJIbpKKvh-xl7lvBECoOnUS3ZWLxfQCKhn7Nf9GvlE7oHfPEaPmWdnxl1vEmW-NpkH2iP3FAImHCZ-fbf6WpgRHQVypu8P3LiJ9mZCz-2BX63u-IXxNMYHGrD08nVM2zjgpYvJ_IwDDVXC_g8-4jiRx_ev2Ytg-oxvnuY5-_bp4_3qc3X75fpmdXVbubqFqQrOQvBOdQsnQPrahEVtjZHLRrSq7F51UC5LYVsjrWzlInQd2KYWrQtq2dbn7OLUO6b4Y4d50lvKDvveDBh3WRdQtqqpW1VQeUJdijknDHpMtDXpoEHoo3u90Uf3-uhen9yX0Lun_p3dov8X-Su7AB9OAJYv94RJZ0c4OPSU0E3aR_pf_2_obZZd</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3052594359</pqid></control><display><type>article</type><title>The tick Ixodes scapularis has five different GPCRs specifically activated by ACP (adipokinetic hormone/corazonin-related peptide)</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Hauser, Frank ; Stebegg, Marisa ; Al-Ribaty, Tara ; Petersen, Lea B. ; Møller, Mads ; Drag, Markus H. ; Sigurdsson, Haraldur H. ; Vilhelm, Martin J. ; Thygesen, Gedske ; Grimmelikhuijzen, Cornelis J.P.</creator><creatorcontrib>Hauser, Frank ; Stebegg, Marisa ; Al-Ribaty, Tara ; Petersen, Lea B. ; Møller, Mads ; Drag, Markus H. ; Sigurdsson, Haraldur H. ; Vilhelm, Martin J. ; Thygesen, Gedske ; Grimmelikhuijzen, Cornelis J.P.</creatorcontrib><description>Insects have about 50 neuropeptide genes and about 70 genes, coding for neuropeptide G protein-coupled receptors (GPCRs). An important, but small family of evolutionarily related insect neuropeptides consists of adipokinetic hormone (AKH), corazonin, and AKH/corazonin-related peptide (ACP). Normally, insects have one specific GPCR for each of these neuropeptides. The tick Ixodes scapularis is not an insect, but belongs to the subphylum Chelicerata, which comprises ticks, scorpions, mites, spiders, and horseshoe crabs. Many of the neuropeptides and neuropeptide GPCRs occurring in insects, also occur in chelicerates, illustrating that insects and chelicerates are evolutionarily closely related. The tick I. scapularis is an ectoparasite and health risk for humans, because it infects its human host with dangerous pathogens during a blood meal. Understanding the biology of ticks will help researchers to prevent tick-borne diseases. By annotating the I. scapularis genome sequence, we previously found that ticks contain as many as five genes, coding for presumed ACP receptors. In the current paper, we cloned these receptors and expressed each of them in Chinese Hamster Ovary (CHO) cells. Each expressed receptor was activated by nanomolar concentrations of ACP, demonstrating that all five receptors were functional ACP receptors. Phylogenetic tree analyses showed that the cloned tick ACP receptors were mostly related to insect ACP receptors and, next, to insect AKH receptors, suggesting that ACP receptor genes and AKH receptor genes originated by gene duplications from a common ancestor. Similar duplications have probably occurred for the ligand genes, during a process of ligand/receptor co-evolution. Interestingly, chelicerates, in contrast to all other arthropods, do not have AKH or AKH receptor genes. Therefore, the ancestor of chelicerates might have lost AKH and AKH receptor genes and functionally replaced them by ACP and ACP receptor genes. For the small family of AKH, ACP, and corazonin receptors and their ligands, gene losses and gene gains occur frequently between the various ecdysozoan clades. Tardigrades, for example, which are well known for their survival in extreme environments, have as many as ten corazonin receptor genes and six corazonin peptide genes, while insects only have one of each, or none. [Display omitted] •The tick Ixodes scapularis is a vector for serious human diseases, such as Lyme.•G protein-coupled receptors (GPCRs) play a central role in the physiology of ticks.•GPCRs are also excellent drug targets to fight ticks.•Ticks have as many as five possible GPCR genes for the tick neuropeptide ACP.•We cloned these GPCRs and deorphanized them as being ACP receptors.</description><identifier>ISSN: 0006-291X</identifier><identifier>ISSN: 1090-2104</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2024.149992</identifier><identifier>PMID: 38714013</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adipokinetic hormone ; Amino Acid Sequence ; Animals ; CHO Cells ; Corazonin ; Cricetulus ; GnRH ; GPCR ; Insect Hormones - genetics ; Insect Hormones - metabolism ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Ixodes - genetics ; Ixodes - metabolism ; Neuropeptides - genetics ; Neuropeptides - metabolism ; Oligopeptides - chemistry ; Oligopeptides - genetics ; Oligopeptides - metabolism ; Phylogeny ; Pyrrolidonecarboxylic Acid - analogs &amp; derivatives ; Pyrrolidonecarboxylic Acid - metabolism ; Receptors, G-Protein-Coupled - genetics ; Receptors, G-Protein-Coupled - metabolism ; Receptors, Neuropeptide - genetics ; Receptors, Neuropeptide - metabolism ; Stress ; Tardigrada</subject><ispartof>Biochemical and biophysical research communications, 2024-07, Vol.717, p.149992, Article 149992</ispartof><rights>2024 The Authors</rights><rights>Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c351t-fcb1fdc967c012d3af73baa284059f73d961c0180b5a2b2527f661b4305cf9853</cites><orcidid>0009-0003-8119-1629 ; 0000-0002-7412-6402 ; 0000-0001-6486-2046 ; 0000-0001-8434-8271 ; 0000-0001-5563-2345</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2024.149992$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38714013$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hauser, Frank</creatorcontrib><creatorcontrib>Stebegg, Marisa</creatorcontrib><creatorcontrib>Al-Ribaty, Tara</creatorcontrib><creatorcontrib>Petersen, Lea B.</creatorcontrib><creatorcontrib>Møller, Mads</creatorcontrib><creatorcontrib>Drag, Markus H.</creatorcontrib><creatorcontrib>Sigurdsson, Haraldur H.</creatorcontrib><creatorcontrib>Vilhelm, Martin J.</creatorcontrib><creatorcontrib>Thygesen, Gedske</creatorcontrib><creatorcontrib>Grimmelikhuijzen, Cornelis J.P.</creatorcontrib><title>The tick Ixodes scapularis has five different GPCRs specifically activated by ACP (adipokinetic hormone/corazonin-related peptide)</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Insects have about 50 neuropeptide genes and about 70 genes, coding for neuropeptide G protein-coupled receptors (GPCRs). An important, but small family of evolutionarily related insect neuropeptides consists of adipokinetic hormone (AKH), corazonin, and AKH/corazonin-related peptide (ACP). Normally, insects have one specific GPCR for each of these neuropeptides. The tick Ixodes scapularis is not an insect, but belongs to the subphylum Chelicerata, which comprises ticks, scorpions, mites, spiders, and horseshoe crabs. Many of the neuropeptides and neuropeptide GPCRs occurring in insects, also occur in chelicerates, illustrating that insects and chelicerates are evolutionarily closely related. The tick I. scapularis is an ectoparasite and health risk for humans, because it infects its human host with dangerous pathogens during a blood meal. Understanding the biology of ticks will help researchers to prevent tick-borne diseases. By annotating the I. scapularis genome sequence, we previously found that ticks contain as many as five genes, coding for presumed ACP receptors. In the current paper, we cloned these receptors and expressed each of them in Chinese Hamster Ovary (CHO) cells. Each expressed receptor was activated by nanomolar concentrations of ACP, demonstrating that all five receptors were functional ACP receptors. Phylogenetic tree analyses showed that the cloned tick ACP receptors were mostly related to insect ACP receptors and, next, to insect AKH receptors, suggesting that ACP receptor genes and AKH receptor genes originated by gene duplications from a common ancestor. Similar duplications have probably occurred for the ligand genes, during a process of ligand/receptor co-evolution. Interestingly, chelicerates, in contrast to all other arthropods, do not have AKH or AKH receptor genes. Therefore, the ancestor of chelicerates might have lost AKH and AKH receptor genes and functionally replaced them by ACP and ACP receptor genes. For the small family of AKH, ACP, and corazonin receptors and their ligands, gene losses and gene gains occur frequently between the various ecdysozoan clades. Tardigrades, for example, which are well known for their survival in extreme environments, have as many as ten corazonin receptor genes and six corazonin peptide genes, while insects only have one of each, or none. [Display omitted] •The tick Ixodes scapularis is a vector for serious human diseases, such as Lyme.•G protein-coupled receptors (GPCRs) play a central role in the physiology of ticks.•GPCRs are also excellent drug targets to fight ticks.•Ticks have as many as five possible GPCR genes for the tick neuropeptide ACP.•We cloned these GPCRs and deorphanized them as being ACP receptors.</description><subject>Adipokinetic hormone</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>CHO Cells</subject><subject>Corazonin</subject><subject>Cricetulus</subject><subject>GnRH</subject><subject>GPCR</subject><subject>Insect Hormones - genetics</subject><subject>Insect Hormones - metabolism</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Ixodes - genetics</subject><subject>Ixodes - metabolism</subject><subject>Neuropeptides - genetics</subject><subject>Neuropeptides - metabolism</subject><subject>Oligopeptides - chemistry</subject><subject>Oligopeptides - genetics</subject><subject>Oligopeptides - metabolism</subject><subject>Phylogeny</subject><subject>Pyrrolidonecarboxylic Acid - analogs &amp; derivatives</subject><subject>Pyrrolidonecarboxylic Acid - metabolism</subject><subject>Receptors, G-Protein-Coupled - genetics</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Receptors, Neuropeptide - genetics</subject><subject>Receptors, Neuropeptide - metabolism</subject><subject>Stress</subject><subject>Tardigrada</subject><issn>0006-291X</issn><issn>1090-2104</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1vEzEQhi0EoqHwBzggH8thU4_3I7HEpYqgVKpEhYrEzfLHWJl0s17sTdRw5JfjkMKR04w0z_tK8zD2FsQcBHSXm7m1yc2lkM0cGqWUfMZmIJSoJIjmOZsJIbpKKvh-xl7lvBECoOnUS3ZWLxfQCKhn7Nf9GvlE7oHfPEaPmWdnxl1vEmW-NpkH2iP3FAImHCZ-fbf6WpgRHQVypu8P3LiJ9mZCz-2BX63u-IXxNMYHGrD08nVM2zjgpYvJ_IwDDVXC_g8-4jiRx_ev2Ytg-oxvnuY5-_bp4_3qc3X75fpmdXVbubqFqQrOQvBOdQsnQPrahEVtjZHLRrSq7F51UC5LYVsjrWzlInQd2KYWrQtq2dbn7OLUO6b4Y4d50lvKDvveDBh3WRdQtqqpW1VQeUJdijknDHpMtDXpoEHoo3u90Uf3-uhen9yX0Lun_p3dov8X-Su7AB9OAJYv94RJZ0c4OPSU0E3aR_pf_2_obZZd</recordid><startdate>20240712</startdate><enddate>20240712</enddate><creator>Hauser, Frank</creator><creator>Stebegg, Marisa</creator><creator>Al-Ribaty, Tara</creator><creator>Petersen, Lea B.</creator><creator>Møller, Mads</creator><creator>Drag, Markus H.</creator><creator>Sigurdsson, Haraldur H.</creator><creator>Vilhelm, Martin J.</creator><creator>Thygesen, Gedske</creator><creator>Grimmelikhuijzen, Cornelis J.P.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0003-8119-1629</orcidid><orcidid>https://orcid.org/0000-0002-7412-6402</orcidid><orcidid>https://orcid.org/0000-0001-6486-2046</orcidid><orcidid>https://orcid.org/0000-0001-8434-8271</orcidid><orcidid>https://orcid.org/0000-0001-5563-2345</orcidid></search><sort><creationdate>20240712</creationdate><title>The tick Ixodes scapularis has five different GPCRs specifically activated by ACP (adipokinetic hormone/corazonin-related peptide)</title><author>Hauser, Frank ; Stebegg, Marisa ; Al-Ribaty, Tara ; Petersen, Lea B. ; Møller, Mads ; Drag, Markus H. ; Sigurdsson, Haraldur H. ; Vilhelm, Martin J. ; Thygesen, Gedske ; Grimmelikhuijzen, Cornelis J.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-fcb1fdc967c012d3af73baa284059f73d961c0180b5a2b2527f661b4305cf9853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adipokinetic hormone</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>CHO Cells</topic><topic>Corazonin</topic><topic>Cricetulus</topic><topic>GnRH</topic><topic>GPCR</topic><topic>Insect Hormones - genetics</topic><topic>Insect Hormones - metabolism</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>Ixodes - genetics</topic><topic>Ixodes - metabolism</topic><topic>Neuropeptides - genetics</topic><topic>Neuropeptides - metabolism</topic><topic>Oligopeptides - chemistry</topic><topic>Oligopeptides - genetics</topic><topic>Oligopeptides - metabolism</topic><topic>Phylogeny</topic><topic>Pyrrolidonecarboxylic Acid - analogs &amp; derivatives</topic><topic>Pyrrolidonecarboxylic Acid - metabolism</topic><topic>Receptors, G-Protein-Coupled - genetics</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>Receptors, Neuropeptide - genetics</topic><topic>Receptors, Neuropeptide - metabolism</topic><topic>Stress</topic><topic>Tardigrada</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hauser, Frank</creatorcontrib><creatorcontrib>Stebegg, Marisa</creatorcontrib><creatorcontrib>Al-Ribaty, Tara</creatorcontrib><creatorcontrib>Petersen, Lea B.</creatorcontrib><creatorcontrib>Møller, Mads</creatorcontrib><creatorcontrib>Drag, Markus H.</creatorcontrib><creatorcontrib>Sigurdsson, Haraldur H.</creatorcontrib><creatorcontrib>Vilhelm, Martin J.</creatorcontrib><creatorcontrib>Thygesen, Gedske</creatorcontrib><creatorcontrib>Grimmelikhuijzen, Cornelis J.P.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hauser, Frank</au><au>Stebegg, Marisa</au><au>Al-Ribaty, Tara</au><au>Petersen, Lea B.</au><au>Møller, Mads</au><au>Drag, Markus H.</au><au>Sigurdsson, Haraldur H.</au><au>Vilhelm, Martin J.</au><au>Thygesen, Gedske</au><au>Grimmelikhuijzen, Cornelis J.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The tick Ixodes scapularis has five different GPCRs specifically activated by ACP (adipokinetic hormone/corazonin-related peptide)</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2024-07-12</date><risdate>2024</risdate><volume>717</volume><spage>149992</spage><pages>149992-</pages><artnum>149992</artnum><issn>0006-291X</issn><issn>1090-2104</issn><eissn>1090-2104</eissn><abstract>Insects have about 50 neuropeptide genes and about 70 genes, coding for neuropeptide G protein-coupled receptors (GPCRs). An important, but small family of evolutionarily related insect neuropeptides consists of adipokinetic hormone (AKH), corazonin, and AKH/corazonin-related peptide (ACP). Normally, insects have one specific GPCR for each of these neuropeptides. The tick Ixodes scapularis is not an insect, but belongs to the subphylum Chelicerata, which comprises ticks, scorpions, mites, spiders, and horseshoe crabs. Many of the neuropeptides and neuropeptide GPCRs occurring in insects, also occur in chelicerates, illustrating that insects and chelicerates are evolutionarily closely related. The tick I. scapularis is an ectoparasite and health risk for humans, because it infects its human host with dangerous pathogens during a blood meal. Understanding the biology of ticks will help researchers to prevent tick-borne diseases. By annotating the I. scapularis genome sequence, we previously found that ticks contain as many as five genes, coding for presumed ACP receptors. In the current paper, we cloned these receptors and expressed each of them in Chinese Hamster Ovary (CHO) cells. Each expressed receptor was activated by nanomolar concentrations of ACP, demonstrating that all five receptors were functional ACP receptors. Phylogenetic tree analyses showed that the cloned tick ACP receptors were mostly related to insect ACP receptors and, next, to insect AKH receptors, suggesting that ACP receptor genes and AKH receptor genes originated by gene duplications from a common ancestor. Similar duplications have probably occurred for the ligand genes, during a process of ligand/receptor co-evolution. Interestingly, chelicerates, in contrast to all other arthropods, do not have AKH or AKH receptor genes. Therefore, the ancestor of chelicerates might have lost AKH and AKH receptor genes and functionally replaced them by ACP and ACP receptor genes. For the small family of AKH, ACP, and corazonin receptors and their ligands, gene losses and gene gains occur frequently between the various ecdysozoan clades. Tardigrades, for example, which are well known for their survival in extreme environments, have as many as ten corazonin receptor genes and six corazonin peptide genes, while insects only have one of each, or none. [Display omitted] •The tick Ixodes scapularis is a vector for serious human diseases, such as Lyme.•G protein-coupled receptors (GPCRs) play a central role in the physiology of ticks.•GPCRs are also excellent drug targets to fight ticks.•Ticks have as many as five possible GPCR genes for the tick neuropeptide ACP.•We cloned these GPCRs and deorphanized them as being ACP receptors.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>38714013</pmid><doi>10.1016/j.bbrc.2024.149992</doi><orcidid>https://orcid.org/0009-0003-8119-1629</orcidid><orcidid>https://orcid.org/0000-0002-7412-6402</orcidid><orcidid>https://orcid.org/0000-0001-6486-2046</orcidid><orcidid>https://orcid.org/0000-0001-8434-8271</orcidid><orcidid>https://orcid.org/0000-0001-5563-2345</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0006-291X
ispartof Biochemical and biophysical research communications, 2024-07, Vol.717, p.149992, Article 149992
issn 0006-291X
1090-2104
1090-2104
language eng
recordid cdi_proquest_miscellaneous_3052594359
source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Adipokinetic hormone
Amino Acid Sequence
Animals
CHO Cells
Corazonin
Cricetulus
GnRH
GPCR
Insect Hormones - genetics
Insect Hormones - metabolism
Insect Proteins - genetics
Insect Proteins - metabolism
Ixodes - genetics
Ixodes - metabolism
Neuropeptides - genetics
Neuropeptides - metabolism
Oligopeptides - chemistry
Oligopeptides - genetics
Oligopeptides - metabolism
Phylogeny
Pyrrolidonecarboxylic Acid - analogs & derivatives
Pyrrolidonecarboxylic Acid - metabolism
Receptors, G-Protein-Coupled - genetics
Receptors, G-Protein-Coupled - metabolism
Receptors, Neuropeptide - genetics
Receptors, Neuropeptide - metabolism
Stress
Tardigrada
title The tick Ixodes scapularis has five different GPCRs specifically activated by ACP (adipokinetic hormone/corazonin-related peptide)
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T21%3A01%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20tick%20Ixodes%20scapularis%20has%20five%20different%20GPCRs%20specifically%20activated%20by%20ACP%20(adipokinetic%20hormone/corazonin-related%20peptide)&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Hauser,%20Frank&rft.date=2024-07-12&rft.volume=717&rft.spage=149992&rft.pages=149992-&rft.artnum=149992&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2024.149992&rft_dat=%3Cproquest_cross%3E3052594359%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3052594359&rft_id=info:pmid/38714013&rft_els_id=S0006291X2400528X&rfr_iscdi=true