Stable Isotope Probing‐nanoFTIR for Quantitation of Cellular Metabolism and Observation of Growth‐Dependent Spectral Features

This study utilizes nanoscale Fourier transform infrared spectroscopy (nanoFTIR) to perform stable isotope probing (SIP) on individual bacteria cells cultured in the presence of 13C‐labelled glucose. SIP‐nanoFTIR simultaneously quantifies single‐cell metabolism through infrared spectroscopy and acqu...

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Veröffentlicht in:	Small (Weinheim an der Bergstrasse, Germany) Germany), 2024-09, Vol.20 (36), p.e2400289-n/a
Hauptverfasser:	Burr, David J., Drauschke, Janina, Kanevche, Katerina, Kümmel, Steffen, Stryhanyuk, Hryhoriy, Heberle, Joachim, Perfumo, Amedea, Elsaesser, Andreas
Format:	Artikel
Sprache:	eng
Schlagworte:	Biophysics Carbon Isotopes - chemistry Cellular structure Coupled modes Escherichia coli - growth & development Escherichia coli - metabolism Fourier transforms Glucose - metabolism Heterogeneity hyperspectral imaging Infrared spectroscopy Isotope Labeling - methods Isotopic enrichment metabolic heterogeneity Metabolism Microorganisms Microscopy, Atomic Force Morphology nanoscopy Nanotechnology - methods Proteins Red shift Spectroscopy, Fourier Transform Infrared - methods Spectrum analysis stable‐isotope probing‐nanoFTIR subcellular resolution
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