Development and Validation of the HPLC Method for Simultaneous Quantification of Glucose and Xylose in Sugar Cane Bagasse Extract
Abstract A validated rapid high-performance liquid chromatography (HPLC)-refractive index (RI) method was developed for the identification and quantification of glucose and xylose in hydrolyzed bagasse extract. The separation of compounds was achieved on Eurokat® H column (300 × 8 mm, 10 μm) at 75°C...
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| Veröffentlicht in: | Journal of chromatographic science 2024-10, Vol.62 (8), p.776-782 |
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| container_title | Journal of chromatographic science |
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| creator | Jafari, Saeedeh Hematian Sourki, Abdollah |
| description | Abstract
A validated rapid high-performance liquid chromatography (HPLC)-refractive index (RI) method was developed for the identification and quantification of glucose and xylose in hydrolyzed bagasse extract. The separation of compounds was achieved on Eurokat® H column (300 × 8 mm, 10 μm) at 75°C, using 0.01 N sulfuric acid solution as mobile phase and 0.6 mL/min as flow rate. The method was validated based on accuracy, precision, linearity, robustness, uncertainty, limit of detection (LOD) and limit of quantification (LOQ). Total chromatographic analysis time per sample was ~11 min. Calibration plots were linear over the concentration ranges 11–100 μg/100 μL for glucose and xylose. The LOD was 0.8 ppm and LOQ was 2.5 ppm. The high recovery and low relative standard deviation confirm the suitability of the method for determination of glucose and xylose in bagasse extract. The proposed HPLC-RI method was accurate, fast and robust and required less run time due to less analytes retention times and allowed optimal energy consumption owing to lower column oven temperature. |
| doi_str_mv | 10.1093/chromsci/bmae018 |
| format | Article |
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A validated rapid high-performance liquid chromatography (HPLC)-refractive index (RI) method was developed for the identification and quantification of glucose and xylose in hydrolyzed bagasse extract. The separation of compounds was achieved on Eurokat® H column (300 × 8 mm, 10 μm) at 75°C, using 0.01 N sulfuric acid solution as mobile phase and 0.6 mL/min as flow rate. The method was validated based on accuracy, precision, linearity, robustness, uncertainty, limit of detection (LOD) and limit of quantification (LOQ). Total chromatographic analysis time per sample was ~11 min. Calibration plots were linear over the concentration ranges 11–100 μg/100 μL for glucose and xylose. The LOD was 0.8 ppm and LOQ was 2.5 ppm. The high recovery and low relative standard deviation confirm the suitability of the method for determination of glucose and xylose in bagasse extract. The proposed HPLC-RI method was accurate, fast and robust and required less run time due to less analytes retention times and allowed optimal energy consumption owing to lower column oven temperature.</description><identifier>ISSN: 0021-9665</identifier><identifier>ISSN: 1945-239X</identifier><identifier>EISSN: 1945-239X</identifier><identifier>DOI: 10.1093/chromsci/bmae018</identifier><identifier>PMID: 38704240</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Cellulose - chemistry ; Chromatography, High Pressure Liquid - methods ; Glucose - analysis ; Limit of Detection ; Linear Models ; Plant Extracts - analysis ; Plant Extracts - chemistry ; Reproducibility of Results ; Saccharum - chemistry ; Xylose - analysis</subject><ispartof>Journal of chromatographic science, 2024-10, Vol.62 (8), p.776-782</ispartof><rights>The Author(s) 2024. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com 2024</rights><rights>The Author(s) 2024. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c260t-2c156bb77235c3314f2dcfcfdd32bf53e39d92d055f53a64228ed7e76cdb8c5d3</cites><orcidid>0000-0002-1355-4403</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38704240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jafari, Saeedeh</creatorcontrib><creatorcontrib>Hematian Sourki, Abdollah</creatorcontrib><title>Development and Validation of the HPLC Method for Simultaneous Quantification of Glucose and Xylose in Sugar Cane Bagasse Extract</title><title>Journal of chromatographic science</title><addtitle>J Chromatogr Sci</addtitle><description>Abstract
A validated rapid high-performance liquid chromatography (HPLC)-refractive index (RI) method was developed for the identification and quantification of glucose and xylose in hydrolyzed bagasse extract. The separation of compounds was achieved on Eurokat® H column (300 × 8 mm, 10 μm) at 75°C, using 0.01 N sulfuric acid solution as mobile phase and 0.6 mL/min as flow rate. The method was validated based on accuracy, precision, linearity, robustness, uncertainty, limit of detection (LOD) and limit of quantification (LOQ). Total chromatographic analysis time per sample was ~11 min. Calibration plots were linear over the concentration ranges 11–100 μg/100 μL for glucose and xylose. The LOD was 0.8 ppm and LOQ was 2.5 ppm. The high recovery and low relative standard deviation confirm the suitability of the method for determination of glucose and xylose in bagasse extract. The proposed HPLC-RI method was accurate, fast and robust and required less run time due to less analytes retention times and allowed optimal energy consumption owing to lower column oven temperature.</description><subject>Cellulose - chemistry</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Glucose - analysis</subject><subject>Limit of Detection</subject><subject>Linear Models</subject><subject>Plant Extracts - analysis</subject><subject>Plant Extracts - chemistry</subject><subject>Reproducibility of Results</subject><subject>Saccharum - chemistry</subject><subject>Xylose - analysis</subject><issn>0021-9665</issn><issn>1945-239X</issn><issn>1945-239X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkElPwzAQhS0EomW5c0I-IqGAlzjLEUJZpCJALOIWOV6oURIH20H0yD8npS1XTjNv9N6T5gPgAKMTjHJ6KmbONl6Y06rhCuFsA4xxHrOI0Px1E4wRIjjKk4SNwI737wuJM7YNRjRLUUxiNAbfF-pT1bZrVBsgbyV84bWRPBjbQqthmCl4fT8t4K0KMyuhtg4-mqavA2-V7T186HkbjDbiL3JV98J69Vv2Oq8Xq2nhY__GHSyGFDznb9wP18lXcFyEPbClee3V_mrugufLyVNxHU3vrm6Ks2kkSIJCRARmSVWlKaFMUIpjTaTQQktJSaUZVTSXOZGIsUHwJCYkUzJVaSJklQkm6S44WvZ2zn70yoeyMV6oul5-UlLEUB5jxOhgRUurcNZ7p3TZOdNwNy8xKhfgyzX4cgV-iByu2vuqUfIvsCY9GI6XBtt3_9f9AIDYkrk</recordid><startdate>20241001</startdate><enddate>20241001</enddate><creator>Jafari, Saeedeh</creator><creator>Hematian Sourki, Abdollah</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1355-4403</orcidid></search><sort><creationdate>20241001</creationdate><title>Development and Validation of the HPLC Method for Simultaneous Quantification of Glucose and Xylose in Sugar Cane Bagasse Extract</title><author>Jafari, Saeedeh ; Hematian Sourki, Abdollah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c260t-2c156bb77235c3314f2dcfcfdd32bf53e39d92d055f53a64228ed7e76cdb8c5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Cellulose - chemistry</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Glucose - analysis</topic><topic>Limit of Detection</topic><topic>Linear Models</topic><topic>Plant Extracts - analysis</topic><topic>Plant Extracts - chemistry</topic><topic>Reproducibility of Results</topic><topic>Saccharum - chemistry</topic><topic>Xylose - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jafari, Saeedeh</creatorcontrib><creatorcontrib>Hematian Sourki, Abdollah</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatographic science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jafari, Saeedeh</au><au>Hematian Sourki, Abdollah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and Validation of the HPLC Method for Simultaneous Quantification of Glucose and Xylose in Sugar Cane Bagasse Extract</atitle><jtitle>Journal of chromatographic science</jtitle><addtitle>J Chromatogr Sci</addtitle><date>2024-10-01</date><risdate>2024</risdate><volume>62</volume><issue>8</issue><spage>776</spage><epage>782</epage><pages>776-782</pages><issn>0021-9665</issn><issn>1945-239X</issn><eissn>1945-239X</eissn><abstract>Abstract
A validated rapid high-performance liquid chromatography (HPLC)-refractive index (RI) method was developed for the identification and quantification of glucose and xylose in hydrolyzed bagasse extract. The separation of compounds was achieved on Eurokat® H column (300 × 8 mm, 10 μm) at 75°C, using 0.01 N sulfuric acid solution as mobile phase and 0.6 mL/min as flow rate. The method was validated based on accuracy, precision, linearity, robustness, uncertainty, limit of detection (LOD) and limit of quantification (LOQ). Total chromatographic analysis time per sample was ~11 min. Calibration plots were linear over the concentration ranges 11–100 μg/100 μL for glucose and xylose. The LOD was 0.8 ppm and LOQ was 2.5 ppm. The high recovery and low relative standard deviation confirm the suitability of the method for determination of glucose and xylose in bagasse extract. The proposed HPLC-RI method was accurate, fast and robust and required less run time due to less analytes retention times and allowed optimal energy consumption owing to lower column oven temperature.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>38704240</pmid><doi>10.1093/chromsci/bmae018</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-1355-4403</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0021-9665 |
| ispartof | Journal of chromatographic science, 2024-10, Vol.62 (8), p.776-782 |
| issn | 0021-9665 1945-239X 1945-239X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3050941053 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Cellulose - chemistry Chromatography, High Pressure Liquid - methods Glucose - analysis Limit of Detection Linear Models Plant Extracts - analysis Plant Extracts - chemistry Reproducibility of Results Saccharum - chemistry Xylose - analysis |
| title | Development and Validation of the HPLC Method for Simultaneous Quantification of Glucose and Xylose in Sugar Cane Bagasse Extract |
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