An ingenious technique based on the usage of fluorone‐based dye; pyrosin B in prucalopride assay in different matrices through an “on–off” dye native fluorescence probe

Prucalopride (PCD), is a modern medication approved by the United States in 2018 to alleviate constipation caused by motility issues. PCD demonstrates a strong affinity and selectivity toward the 5‐HT4 receptor. The study here introduces a feasible, direct, non‐extractive, and affordable pathway for...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Luminescence (Chichester, England) England), 2024-05, Vol.39 (5), p.e4752-n/a
Hauptverfasser: Abu‐hassan, Ahmed A., Mahdi, Wael A., Alshehri, Sultan, El Hamd, Mohamed A.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page n/a
container_issue 5
container_start_page e4752
container_title Luminescence (Chichester, England)
container_volume 39
creator Abu‐hassan, Ahmed A.
Mahdi, Wael A.
Alshehri, Sultan
El Hamd, Mohamed A.
description Prucalopride (PCD), is a modern medication approved by the United States in 2018 to alleviate constipation caused by motility issues. PCD demonstrates a strong affinity and selectivity toward the 5‐HT4 receptor. The study here introduces a feasible, direct, non‐extractive, and affordable pathway for PCD analytical tracking. The fluorimetric study is based on the on–off effect on the emission amplitude of fluorone‐based dye (pyrosin B). In a one‐pot experiment, the complex between PCD and pyrosin B is formed instantly in an acidic medium. Correlation between decreased pyrosin B emission and PCD concentrations provides a linear calibration plot from 50 to 900 ng/mL. PCD–dye complex system affecting variables were meticulously tuned. The values of the estimated limit of quantitation and limit of detection for the current methodology were 47.5 and 15.7 ng/mL, respectively. Conformity of the strategy validity was achieved by a comprehensive study of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use criteria. The method was convincingly applied for PCD assay in tablets and content uniformity investigation. Furthermore, PCD tracking in the spiked biological fluid was applied. Finally, the method uses distilled water as dispersing medium which rise accommodation with the green chemistry principle. Simple, accurate, and non‐extractive spectrofluorimetric method for the determination of prucalopride (PCD). The strategy utilizes fluorescence quenching phenomena resulting from the dual complex association between pyrosin B and PCD. The method was applied successfully for the dosage form and human plasma analysis. The method was validated and applied according to the instructions of ICH and FDA guidelines.
doi_str_mv 10.1002/bio.4752
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3050934851</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3061476737</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2252-176a88a5187ee0eda4c76d8df46c79d2b0bfbe2fff5208d8f51a545b65b4be973</originalsourceid><addsrcrecordid>eNp1kctq3DAUhkVpaNK00Ccogm66cSLL1sV0lYReAoFs2rXR5WhGwSNNJTvFu3mEQrftO_SZ5kmq6SShBLrSQXx85_D_CL2qyUlNCD3VPp60gtEn6KhmlFaCts3Th7lhh-h5zjeEEM559wwdNpJ3Qgh5hH6fBezDAoKPU8YjmGXwXyfAWmWwOAY8LgFPWS0AR4fdMMUUA2w33_eAneEdXs8pZh_weTHhdZqMGuI6eQtY5azm3a_1zkGCMOKVGpM3UHYtU5wWS6wC3m5-xrDd_IjObTe_dlIc1OhvYb8QsoFgoKijhhfowKkhw8u79xh9-fD-88Wn6ur64-XF2VVlKGW0qgVXUipWSwFAwKrWCG6ldS03orNUE-00UOcco0Ra6VitWMs0Z7rV0InmGL3de8vWEkge-5UvdwyDClCi6hvCSNe0ktUFffMIvYlTCuW6QvG6FVw0_whNSSsncH3JaKXS3Nek37XYlxb7XYsFfX0nnPQK7AN4X1sBqj3wzQ8w_1fUn19e_xX-AaG9rSk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3061476737</pqid></control><display><type>article</type><title>An ingenious technique based on the usage of fluorone‐based dye; pyrosin B in prucalopride assay in different matrices through an “on–off” dye native fluorescence probe</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Abu‐hassan, Ahmed A. ; Mahdi, Wael A. ; Alshehri, Sultan ; El Hamd, Mohamed A.</creator><creatorcontrib>Abu‐hassan, Ahmed A. ; Mahdi, Wael A. ; Alshehri, Sultan ; El Hamd, Mohamed A.</creatorcontrib><description>Prucalopride (PCD), is a modern medication approved by the United States in 2018 to alleviate constipation caused by motility issues. PCD demonstrates a strong affinity and selectivity toward the 5‐HT4 receptor. The study here introduces a feasible, direct, non‐extractive, and affordable pathway for PCD analytical tracking. The fluorimetric study is based on the on–off effect on the emission amplitude of fluorone‐based dye (pyrosin B). In a one‐pot experiment, the complex between PCD and pyrosin B is formed instantly in an acidic medium. Correlation between decreased pyrosin B emission and PCD concentrations provides a linear calibration plot from 50 to 900 ng/mL. PCD–dye complex system affecting variables were meticulously tuned. The values of the estimated limit of quantitation and limit of detection for the current methodology were 47.5 and 15.7 ng/mL, respectively. Conformity of the strategy validity was achieved by a comprehensive study of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use criteria. The method was convincingly applied for PCD assay in tablets and content uniformity investigation. Furthermore, PCD tracking in the spiked biological fluid was applied. Finally, the method uses distilled water as dispersing medium which rise accommodation with the green chemistry principle. Simple, accurate, and non‐extractive spectrofluorimetric method for the determination of prucalopride (PCD). The strategy utilizes fluorescence quenching phenomena resulting from the dual complex association between pyrosin B and PCD. The method was applied successfully for the dosage form and human plasma analysis. The method was validated and applied according to the instructions of ICH and FDA guidelines.</description><identifier>ISSN: 1522-7235</identifier><identifier>EISSN: 1522-7243</identifier><identifier>DOI: 10.1002/bio.4752</identifier><identifier>PMID: 38697778</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>5‐HT4 ; Benzofurans - analysis ; Benzofurans - chemistry ; biological fluid ; Complex systems ; Complex variables ; Constipation ; Distilled water ; Dyes ; Emission ; Emissions ; Fluorescence ; Fluorescent Dyes - chemistry ; Fluorescent indicators ; Green chemistry ; green chemistry principle ; Humans ; Limit of Detection ; Molecular Structure ; prucalopride ; Spectrometry, Fluorescence ; Tracking</subject><ispartof>Luminescence (Chichester, England), 2024-05, Vol.39 (5), p.e4752-n/a</ispartof><rights>2024 John Wiley &amp; Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2252-176a88a5187ee0eda4c76d8df46c79d2b0bfbe2fff5208d8f51a545b65b4be973</cites><orcidid>0000-0002-9774-0587 ; 0000-0001-8524-6867</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbio.4752$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbio.4752$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38697778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abu‐hassan, Ahmed A.</creatorcontrib><creatorcontrib>Mahdi, Wael A.</creatorcontrib><creatorcontrib>Alshehri, Sultan</creatorcontrib><creatorcontrib>El Hamd, Mohamed A.</creatorcontrib><title>An ingenious technique based on the usage of fluorone‐based dye; pyrosin B in prucalopride assay in different matrices through an “on–off” dye native fluorescence probe</title><title>Luminescence (Chichester, England)</title><addtitle>Luminescence</addtitle><description>Prucalopride (PCD), is a modern medication approved by the United States in 2018 to alleviate constipation caused by motility issues. PCD demonstrates a strong affinity and selectivity toward the 5‐HT4 receptor. The study here introduces a feasible, direct, non‐extractive, and affordable pathway for PCD analytical tracking. The fluorimetric study is based on the on–off effect on the emission amplitude of fluorone‐based dye (pyrosin B). In a one‐pot experiment, the complex between PCD and pyrosin B is formed instantly in an acidic medium. Correlation between decreased pyrosin B emission and PCD concentrations provides a linear calibration plot from 50 to 900 ng/mL. PCD–dye complex system affecting variables were meticulously tuned. The values of the estimated limit of quantitation and limit of detection for the current methodology were 47.5 and 15.7 ng/mL, respectively. Conformity of the strategy validity was achieved by a comprehensive study of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use criteria. The method was convincingly applied for PCD assay in tablets and content uniformity investigation. Furthermore, PCD tracking in the spiked biological fluid was applied. Finally, the method uses distilled water as dispersing medium which rise accommodation with the green chemistry principle. Simple, accurate, and non‐extractive spectrofluorimetric method for the determination of prucalopride (PCD). The strategy utilizes fluorescence quenching phenomena resulting from the dual complex association between pyrosin B and PCD. The method was applied successfully for the dosage form and human plasma analysis. The method was validated and applied according to the instructions of ICH and FDA guidelines.</description><subject>5‐HT4</subject><subject>Benzofurans - analysis</subject><subject>Benzofurans - chemistry</subject><subject>biological fluid</subject><subject>Complex systems</subject><subject>Complex variables</subject><subject>Constipation</subject><subject>Distilled water</subject><subject>Dyes</subject><subject>Emission</subject><subject>Emissions</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent indicators</subject><subject>Green chemistry</subject><subject>green chemistry principle</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Molecular Structure</subject><subject>prucalopride</subject><subject>Spectrometry, Fluorescence</subject><subject>Tracking</subject><issn>1522-7235</issn><issn>1522-7243</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctq3DAUhkVpaNK00Ccogm66cSLL1sV0lYReAoFs2rXR5WhGwSNNJTvFu3mEQrftO_SZ5kmq6SShBLrSQXx85_D_CL2qyUlNCD3VPp60gtEn6KhmlFaCts3Th7lhh-h5zjeEEM559wwdNpJ3Qgh5hH6fBezDAoKPU8YjmGXwXyfAWmWwOAY8LgFPWS0AR4fdMMUUA2w33_eAneEdXs8pZh_weTHhdZqMGuI6eQtY5azm3a_1zkGCMOKVGpM3UHYtU5wWS6wC3m5-xrDd_IjObTe_dlIc1OhvYb8QsoFgoKijhhfowKkhw8u79xh9-fD-88Wn6ur64-XF2VVlKGW0qgVXUipWSwFAwKrWCG6ldS03orNUE-00UOcco0Ra6VitWMs0Z7rV0InmGL3de8vWEkge-5UvdwyDClCi6hvCSNe0ktUFffMIvYlTCuW6QvG6FVw0_whNSSsncH3JaKXS3Nek37XYlxb7XYsFfX0nnPQK7AN4X1sBqj3wzQ8w_1fUn19e_xX-AaG9rSk</recordid><startdate>202405</startdate><enddate>202405</enddate><creator>Abu‐hassan, Ahmed A.</creator><creator>Mahdi, Wael A.</creator><creator>Alshehri, Sultan</creator><creator>El Hamd, Mohamed A.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H95</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L.G</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9774-0587</orcidid><orcidid>https://orcid.org/0000-0001-8524-6867</orcidid></search><sort><creationdate>202405</creationdate><title>An ingenious technique based on the usage of fluorone‐based dye; pyrosin B in prucalopride assay in different matrices through an “on–off” dye native fluorescence probe</title><author>Abu‐hassan, Ahmed A. ; Mahdi, Wael A. ; Alshehri, Sultan ; El Hamd, Mohamed A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2252-176a88a5187ee0eda4c76d8df46c79d2b0bfbe2fff5208d8f51a545b65b4be973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>5‐HT4</topic><topic>Benzofurans - analysis</topic><topic>Benzofurans - chemistry</topic><topic>biological fluid</topic><topic>Complex systems</topic><topic>Complex variables</topic><topic>Constipation</topic><topic>Distilled water</topic><topic>Dyes</topic><topic>Emission</topic><topic>Emissions</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent indicators</topic><topic>Green chemistry</topic><topic>green chemistry principle</topic><topic>Humans</topic><topic>Limit of Detection</topic><topic>Molecular Structure</topic><topic>prucalopride</topic><topic>Spectrometry, Fluorescence</topic><topic>Tracking</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abu‐hassan, Ahmed A.</creatorcontrib><creatorcontrib>Mahdi, Wael A.</creatorcontrib><creatorcontrib>Alshehri, Sultan</creatorcontrib><creatorcontrib>El Hamd, Mohamed A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics &amp; Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical &amp; Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>ANTE: Abstracts in New Technology &amp; Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts – Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Luminescence (Chichester, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abu‐hassan, Ahmed A.</au><au>Mahdi, Wael A.</au><au>Alshehri, Sultan</au><au>El Hamd, Mohamed A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An ingenious technique based on the usage of fluorone‐based dye; pyrosin B in prucalopride assay in different matrices through an “on–off” dye native fluorescence probe</atitle><jtitle>Luminescence (Chichester, England)</jtitle><addtitle>Luminescence</addtitle><date>2024-05</date><risdate>2024</risdate><volume>39</volume><issue>5</issue><spage>e4752</spage><epage>n/a</epage><pages>e4752-n/a</pages><issn>1522-7235</issn><eissn>1522-7243</eissn><abstract>Prucalopride (PCD), is a modern medication approved by the United States in 2018 to alleviate constipation caused by motility issues. PCD demonstrates a strong affinity and selectivity toward the 5‐HT4 receptor. The study here introduces a feasible, direct, non‐extractive, and affordable pathway for PCD analytical tracking. The fluorimetric study is based on the on–off effect on the emission amplitude of fluorone‐based dye (pyrosin B). In a one‐pot experiment, the complex between PCD and pyrosin B is formed instantly in an acidic medium. Correlation between decreased pyrosin B emission and PCD concentrations provides a linear calibration plot from 50 to 900 ng/mL. PCD–dye complex system affecting variables were meticulously tuned. The values of the estimated limit of quantitation and limit of detection for the current methodology were 47.5 and 15.7 ng/mL, respectively. Conformity of the strategy validity was achieved by a comprehensive study of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use criteria. The method was convincingly applied for PCD assay in tablets and content uniformity investigation. Furthermore, PCD tracking in the spiked biological fluid was applied. Finally, the method uses distilled water as dispersing medium which rise accommodation with the green chemistry principle. Simple, accurate, and non‐extractive spectrofluorimetric method for the determination of prucalopride (PCD). The strategy utilizes fluorescence quenching phenomena resulting from the dual complex association between pyrosin B and PCD. The method was applied successfully for the dosage form and human plasma analysis. The method was validated and applied according to the instructions of ICH and FDA guidelines.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>38697778</pmid><doi>10.1002/bio.4752</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9774-0587</orcidid><orcidid>https://orcid.org/0000-0001-8524-6867</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 1522-7235
ispartof Luminescence (Chichester, England), 2024-05, Vol.39 (5), p.e4752-n/a
issn 1522-7235
1522-7243
language eng
recordid cdi_proquest_miscellaneous_3050934851
source MEDLINE; Access via Wiley Online Library
subjects 5‐HT4
Benzofurans - analysis
Benzofurans - chemistry
biological fluid
Complex systems
Complex variables
Constipation
Distilled water
Dyes
Emission
Emissions
Fluorescence
Fluorescent Dyes - chemistry
Fluorescent indicators
Green chemistry
green chemistry principle
Humans
Limit of Detection
Molecular Structure
prucalopride
Spectrometry, Fluorescence
Tracking
title An ingenious technique based on the usage of fluorone‐based dye; pyrosin B in prucalopride assay in different matrices through an “on–off” dye native fluorescence probe
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T02%3A26%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20ingenious%20technique%20based%20on%20the%20usage%20of%20fluorone%E2%80%90based%20dye;%20pyrosin%20B%20in%20prucalopride%20assay%20in%20different%20matrices%20through%20an%20%E2%80%9Con%E2%80%93off%E2%80%9D%20dye%20native%20fluorescence%20probe&rft.jtitle=Luminescence%20(Chichester,%20England)&rft.au=Abu%E2%80%90hassan,%20Ahmed%20A.&rft.date=2024-05&rft.volume=39&rft.issue=5&rft.spage=e4752&rft.epage=n/a&rft.pages=e4752-n/a&rft.issn=1522-7235&rft.eissn=1522-7243&rft_id=info:doi/10.1002/bio.4752&rft_dat=%3Cproquest_cross%3E3061476737%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3061476737&rft_id=info:pmid/38697778&rfr_iscdi=true