Different Detection Strategies of Pediocin-Like Produced by Pediococcus pentosaceus

Different Detection Strategies of Pediocin-Like Produced by Pediococcus pentosaceus

In this study, Pediococcus pentosaceus C-2-1 and C23221 contained genes encoding penocin and pediocin PA-1, mined by antiSMASH. The penocin structural gene pedA from Pediococcus pentosaceus C-2-1 was successfully expressed in Escherichia coli BL21. The presence of a 6.5 kDa recombinant penocin was c...

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Veröffentlicht in:Probiotics and antimicrobial proteins 2024-05
Hauptverfasser: Li, Hongbiao, Zheng, Huojian, Li, Lanxin, Lin, Yi, Cheng, Qiuxiang, Wang, Liping, Zhao, Yueliang, Liu, Zhenmin
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container_title Probiotics and antimicrobial proteins
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creator Li, Hongbiao
Zheng, Huojian
Li, Lanxin
Lin, Yi
Cheng, Qiuxiang
Wang, Liping
Zhao, Yueliang
Liu, Zhenmin
description In this study, Pediococcus pentosaceus C-2-1 and C23221 contained genes encoding penocin and pediocin PA-1, mined by antiSMASH. The penocin structural gene pedA from Pediococcus pentosaceus C-2-1 was successfully expressed in Escherichia coli BL21. The presence of a 6.5 kDa recombinant penocin was confirmed by Tricine-SDS-PAGE, and the specific activity increased by 1.54-fold. The bacteriocins produced by Pediococcus pentosaceus C23221 were purified using acetic ether extraction, Sepharose Fast Flow, Sephadex G-25 gel chromatography, and reversed-phase high-performance liquid chromatography (RP-HPLC); the amino acid sequence of this bacteriocin was identical to pediocin PA-1 by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), which confirmed the expression of pediocin PA-1 gene; and the specific activity increased by 24.39-fold. The heterologous expression and purification of bacteriocins have proved the expression of pediocin-like produced by Pediococcus pentosaceus. This provides a theoretical basis for the subsequent development and application of pediocin-like.
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