Single-Atom Iron Doped Carbon Dots with Highly Efficient Electrochemiluminescence for Ultrasensitive Detection of MicroRNAs
Herein, single-atom iron doped carbon dots (SA Fe-CDs) were successfully prepared as novel electrochemiluminescence (ECL) emitters with high ECL efficiency, and a biosensor was constructed to ultrasensitively detect microRNA-222 (miRNA-222). Importantly, compared with the conventional without single...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2024-05, Vol.96 (19), p.7516-7523 |
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| creator | Liao, Ye-Yu Guo, Yu-Zhuo Liu, Jia-Li Shen, Zhao-Chen Chai, Ya-Qin Yuan, Ruo |
| description | Herein, single-atom iron doped carbon dots (SA Fe-CDs) were successfully prepared as novel electrochemiluminescence (ECL) emitters with high ECL efficiency, and a biosensor was constructed to ultrasensitively detect microRNA-222 (miRNA-222). Importantly, compared with the conventional without single-atom doped CDs with low ECL efficiency, SA Fe-CDs exhibited strong ECL efficiency, in which single-atom iron as an advanced coreactant accelerator could significantly enhance the generation of reactive oxygen species (ROS) from the coreactant S2O8 2– for improving the ECL efficiency. Moreover, a neoteric amplification strategy combining the improved strand displacement amplification with Nt.BbvCI enzyme-induced target amplification (ISDA-EITA) could produce 4 output DNAs in every cycle, which greatly improved the amplification efficiency. Thus, a useful ECL biosensor was built with a detection limit of 16.60 aM in the range of 100 aM to 1 nM for detecting traces of miRNA-222. In addition, miRNA-222 in cancer cell lysate (MHCC-97L) was successfully detected by using the ECL biosensor. Therefore, this strategy provides highly efficient single-atom doped ECL emitters for the construction of sensitive ECL biosensing platforms in the biological field and clinical diagnosis. |
| doi_str_mv | 10.1021/acs.analchem.4c00225 |
| format | Article |
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Importantly, compared with the conventional without single-atom doped CDs with low ECL efficiency, SA Fe-CDs exhibited strong ECL efficiency, in which single-atom iron as an advanced coreactant accelerator could significantly enhance the generation of reactive oxygen species (ROS) from the coreactant S2O8 2– for improving the ECL efficiency. Moreover, a neoteric amplification strategy combining the improved strand displacement amplification with Nt.BbvCI enzyme-induced target amplification (ISDA-EITA) could produce 4 output DNAs in every cycle, which greatly improved the amplification efficiency. Thus, a useful ECL biosensor was built with a detection limit of 16.60 aM in the range of 100 aM to 1 nM for detecting traces of miRNA-222. In addition, miRNA-222 in cancer cell lysate (MHCC-97L) was successfully detected by using the ECL biosensor. Therefore, this strategy provides highly efficient single-atom doped ECL emitters for the construction of sensitive ECL biosensing platforms in the biological field and clinical diagnosis.</description><identifier>ISSN: 0003-2700</identifier><identifier>ISSN: 1520-6882</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.4c00225</identifier><identifier>PMID: 38691765</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Amplification ; Biosensing Techniques - methods ; Biosensors ; Carbon ; Carbon - chemistry ; Carbon dots ; Efficiency ; Electrochemical Techniques - methods ; Electrochemiluminescence ; Emitters ; Humans ; Iron ; Iron - chemistry ; Limit of Detection ; Luminescent Measurements ; MicroRNAs ; MicroRNAs - analysis ; miRNA ; Quantum Dots - chemistry ; Reactive oxygen species</subject><ispartof>Analytical chemistry (Washington), 2024-05, Vol.96 (19), p.7516-7523</ispartof><rights>2024 American Chemical Society</rights><rights>Copyright American Chemical Society May 14, 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a325t-da44ec88ac3f2507290c26a323b8313f48b950e0c6eb6211d20be816e9bb5d563</cites><orcidid>0000-0003-4392-9592 ; 0000-0003-3664-6236 ; 0000-0003-4749-3079</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.4c00225$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.4c00225$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38691765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liao, Ye-Yu</creatorcontrib><creatorcontrib>Guo, Yu-Zhuo</creatorcontrib><creatorcontrib>Liu, Jia-Li</creatorcontrib><creatorcontrib>Shen, Zhao-Chen</creatorcontrib><creatorcontrib>Chai, Ya-Qin</creatorcontrib><creatorcontrib>Yuan, Ruo</creatorcontrib><title>Single-Atom Iron Doped Carbon Dots with Highly Efficient Electrochemiluminescence for Ultrasensitive Detection of MicroRNAs</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. 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Thus, a useful ECL biosensor was built with a detection limit of 16.60 aM in the range of 100 aM to 1 nM for detecting traces of miRNA-222. In addition, miRNA-222 in cancer cell lysate (MHCC-97L) was successfully detected by using the ECL biosensor. 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Guo, Yu-Zhuo ; Liu, Jia-Li ; Shen, Zhao-Chen ; Chai, Ya-Qin ; Yuan, Ruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a325t-da44ec88ac3f2507290c26a323b8313f48b950e0c6eb6211d20be816e9bb5d563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Amplification</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Carbon</topic><topic>Carbon - chemistry</topic><topic>Carbon dots</topic><topic>Efficiency</topic><topic>Electrochemical Techniques - methods</topic><topic>Electrochemiluminescence</topic><topic>Emitters</topic><topic>Humans</topic><topic>Iron</topic><topic>Iron - chemistry</topic><topic>Limit of Detection</topic><topic>Luminescent Measurements</topic><topic>MicroRNAs</topic><topic>MicroRNAs - analysis</topic><topic>miRNA</topic><topic>Quantum Dots - chemistry</topic><topic>Reactive oxygen species</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liao, Ye-Yu</creatorcontrib><creatorcontrib>Guo, Yu-Zhuo</creatorcontrib><creatorcontrib>Liu, Jia-Li</creatorcontrib><creatorcontrib>Shen, Zhao-Chen</creatorcontrib><creatorcontrib>Chai, Ya-Qin</creatorcontrib><creatorcontrib>Yuan, Ruo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liao, Ye-Yu</au><au>Guo, Yu-Zhuo</au><au>Liu, Jia-Li</au><au>Shen, Zhao-Chen</au><au>Chai, Ya-Qin</au><au>Yuan, Ruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-Atom Iron Doped Carbon Dots with Highly Efficient Electrochemiluminescence for Ultrasensitive Detection of MicroRNAs</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2024-05-14</date><risdate>2024</risdate><volume>96</volume><issue>19</issue><spage>7516</spage><epage>7523</epage><pages>7516-7523</pages><issn>0003-2700</issn><issn>1520-6882</issn><eissn>1520-6882</eissn><abstract>Herein, single-atom iron doped carbon dots (SA Fe-CDs) were successfully prepared as novel electrochemiluminescence (ECL) emitters with high ECL efficiency, and a biosensor was constructed to ultrasensitively detect microRNA-222 (miRNA-222). Importantly, compared with the conventional without single-atom doped CDs with low ECL efficiency, SA Fe-CDs exhibited strong ECL efficiency, in which single-atom iron as an advanced coreactant accelerator could significantly enhance the generation of reactive oxygen species (ROS) from the coreactant S2O8 2– for improving the ECL efficiency. Moreover, a neoteric amplification strategy combining the improved strand displacement amplification with Nt.BbvCI enzyme-induced target amplification (ISDA-EITA) could produce 4 output DNAs in every cycle, which greatly improved the amplification efficiency. Thus, a useful ECL biosensor was built with a detection limit of 16.60 aM in the range of 100 aM to 1 nM for detecting traces of miRNA-222. In addition, miRNA-222 in cancer cell lysate (MHCC-97L) was successfully detected by using the ECL biosensor. Therefore, this strategy provides highly efficient single-atom doped ECL emitters for the construction of sensitive ECL biosensing platforms in the biological field and clinical diagnosis.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>38691765</pmid><doi>10.1021/acs.analchem.4c00225</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-4392-9592</orcidid><orcidid>https://orcid.org/0000-0003-3664-6236</orcidid><orcidid>https://orcid.org/0000-0003-4749-3079</orcidid></addata></record> |
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| subjects | Amplification Biosensing Techniques - methods Biosensors Carbon Carbon - chemistry Carbon dots Efficiency Electrochemical Techniques - methods Electrochemiluminescence Emitters Humans Iron Iron - chemistry Limit of Detection Luminescent Measurements MicroRNAs MicroRNAs - analysis miRNA Quantum Dots - chemistry Reactive oxygen species |
| title | Single-Atom Iron Doped Carbon Dots with Highly Efficient Electrochemiluminescence for Ultrasensitive Detection of MicroRNAs |
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