Dual CRISPR/Cas13a Cascade Strand Displacement-Triggered Transcription for Point-of-Care Detection of Plasmodium in Asymptomatic Malaria
Asymptomatic infections of Plasmodium parasites are major obstacles to malaria control and elimination. A sensitive, specific, and user-friendly method is urgently needed for point-of-care (POC) Plasmodium diagnostics in asymptomatic malaria, especially in resource-limited settings. In this work, we...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2024-05, Vol.96 (19), p.7524-7531 |
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| creator | Pian, Hongru Wang, Hui Wang, Honghong Li, Zhengping |
| description | Asymptomatic infections of Plasmodium parasites are major obstacles to malaria control and elimination. A sensitive, specific, and user-friendly method is urgently needed for point-of-care (POC) Plasmodium diagnostics in asymptomatic malaria, especially in resource-limited settings. In this work, we present a POC method (termed Cas13a-SDT) based on the cascade sequence recognition and signal amplification of dual Cas13a trans-cleavage and strand displacement-triggered transcription (SDT). Cas13a-SDT not only achieves exceptional specificity in discriminating the target RNA from nontarget RNAs with any cross-interaction but also meets the sensitivity criterion set by the World Health Organization (WHO) for effective malaria detection. Remarkably, this novel method was successfully applied to screen malaria in asymptomatic infections from clinical samples. The proposed method provides a user-friendly and visually interpretable output mode while maintaining high accuracy and reliability comparable to RT-PCR. These excellent features demonstrate the significant potential of Cas13a-SDT for POC diagnosis of Plasmodium infections, laying a vital foundation for advancing malaria control and elimination efforts. |
| doi_str_mv | 10.1021/acs.analchem.4c00230 |
| format | Article |
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A sensitive, specific, and user-friendly method is urgently needed for point-of-care (POC) Plasmodium diagnostics in asymptomatic malaria, especially in resource-limited settings. In this work, we present a POC method (termed Cas13a-SDT) based on the cascade sequence recognition and signal amplification of dual Cas13a trans-cleavage and strand displacement-triggered transcription (SDT). Cas13a-SDT not only achieves exceptional specificity in discriminating the target RNA from nontarget RNAs with any cross-interaction but also meets the sensitivity criterion set by the World Health Organization (WHO) for effective malaria detection. Remarkably, this novel method was successfully applied to screen malaria in asymptomatic infections from clinical samples. The proposed method provides a user-friendly and visually interpretable output mode while maintaining high accuracy and reliability comparable to RT-PCR. 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Chem</addtitle><description>Asymptomatic infections of Plasmodium parasites are major obstacles to malaria control and elimination. A sensitive, specific, and user-friendly method is urgently needed for point-of-care (POC) Plasmodium diagnostics in asymptomatic malaria, especially in resource-limited settings. In this work, we present a POC method (termed Cas13a-SDT) based on the cascade sequence recognition and signal amplification of dual Cas13a trans-cleavage and strand displacement-triggered transcription (SDT). Cas13a-SDT not only achieves exceptional specificity in discriminating the target RNA from nontarget RNAs with any cross-interaction but also meets the sensitivity criterion set by the World Health Organization (WHO) for effective malaria detection. Remarkably, this novel method was successfully applied to screen malaria in asymptomatic infections from clinical samples. The proposed method provides a user-friendly and visually interpretable output mode while maintaining high accuracy and reliability comparable to RT-PCR. These excellent features demonstrate the significant potential of Cas13a-SDT for POC diagnosis of Plasmodium infections, laying a vital foundation for advancing malaria control and elimination efforts.</description><subject>analytical chemistry</subject><subject>Asymptomatic</subject><subject>CRISPR</subject><subject>CRISPR-Cas Systems - genetics</subject><subject>diagnostic techniques</subject><subject>Humans</subject><subject>Infections</subject><subject>Malaria</subject><subject>Malaria - diagnosis</subject><subject>Malaria - parasitology</subject><subject>Parasites</subject><subject>Plasmodium</subject><subject>Plasmodium - genetics</subject><subject>Plasmodium - isolation & purification</subject><subject>Point-of-Care Systems</subject><subject>RNA</subject><subject>Transcription, Genetic</subject><subject>Vector-borne diseases</subject><subject>World Health Organization</subject><issn>0003-2700</issn><issn>1520-6882</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EotvCP0DIEhcu2Y49-dpjtVtKpSJW7XKOJs6kuEriYCeH_gN-Nt7utgcOcBrJ87yvNXqE-KBgqUCrczJhSQN15if3y9QAaIRXYqEyDUlelvq1WAAAJroAOBGnITwAKAUqfytOsMxXWZFlC_F7M1Mn17fXd9vb8zUFhSTjMNSwvJs8DY3c2DB2ZLjnYUp23t7fs-dG7uIyGG_HybpBts7LrbORcG2yJs9ywxObp51r5baj0LvGzr20g7wIj_04uZ4ma-Q36shbeifetNQFfn-cZ-LHl8vd-mty8_3qen1xkxBm5ZQgtgpzLhlS1lynpmFVruLxNRdsalUjYnxgoxQhaVOgwVLVRVkUKTJpPBOfD72jd79mDlPV22C462hgN4cKVYalzlNc_R-FDFSRYbZHP_2FPrjZRztPVK4xKzREKj1QxrsQPLfV6G1P_rFSUO2lVlFq9Sy1OkqNsY_H8rnuuXkJPVuMAByAffzl4392_gHYWLBK</recordid><startdate>20240514</startdate><enddate>20240514</enddate><creator>Pian, Hongru</creator><creator>Wang, Hui</creator><creator>Wang, Honghong</creator><creator>Li, Zhengping</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-5116-1977</orcidid><orcidid>https://orcid.org/0000-0002-8535-5358</orcidid><orcidid>https://orcid.org/0000-0002-7573-8822</orcidid></search><sort><creationdate>20240514</creationdate><title>Dual CRISPR/Cas13a Cascade Strand Displacement-Triggered Transcription for Point-of-Care Detection of Plasmodium in Asymptomatic Malaria</title><author>Pian, Hongru ; 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| subjects | analytical chemistry Asymptomatic CRISPR CRISPR-Cas Systems - genetics diagnostic techniques Humans Infections Malaria Malaria - diagnosis Malaria - parasitology Parasites Plasmodium Plasmodium - genetics Plasmodium - isolation & purification Point-of-Care Systems RNA Transcription, Genetic Vector-borne diseases World Health Organization |
| title | Dual CRISPR/Cas13a Cascade Strand Displacement-Triggered Transcription for Point-of-Care Detection of Plasmodium in Asymptomatic Malaria |
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