Establishment of RNAi-Mediated Pest Control Method for Red Imported Fire Ant, Solenopsis invicta
RNAi plays a crucial role in insect gene function research and pest control field. Nonetheless, the variable efficiency of RNAi across diverse insects and off-target effects also limited its further application. In this study, we cloned six essential housekeeping genes from Solenopsis invicta and co...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2024-05, Vol.72 (19), p.10936-10943 |
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| container_issue | 19 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Wang, Jin-da Chen, Yao-hui Zhang, Ya-xin Lin, Jin-wen Gao, San-ji Tang, Bao-zhen Hou, You-ming |
| description | RNAi plays a crucial role in insect gene function research and pest control field. Nonetheless, the variable efficiency of RNAi across diverse insects and off-target effects also limited its further application. In this study, we cloned six essential housekeeping genes from Solenopsis invicta and conducted RNAi experiments by orally administering dsRNA. Then, we found that mixing with liposomes significantly enhanced the RNAi efficiency by targeting for SiV-ATPaseE. Additionally, we observed a certain lethal effect of this dsRNA on queens by our established RNAi system. Furthermore, no strict sequence-related off-target effects were detected. Finally, the RNAi effect of large-scale bacteria expressing dsRNA was successfully confirmed for controlling S. invicta. In summary, this study established an RNAi system for S. invicta and provided a research template for the future development of nucleic acid drugs based on RNAi. |
| doi_str_mv | 10.1021/acs.jafc.4c00654 |
| format | Article |
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Nonetheless, the variable efficiency of RNAi across diverse insects and off-target effects also limited its further application. In this study, we cloned six essential housekeeping genes from Solenopsis invicta and conducted RNAi experiments by orally administering dsRNA. Then, we found that mixing with liposomes significantly enhanced the RNAi efficiency by targeting for SiV-ATPaseE. Additionally, we observed a certain lethal effect of this dsRNA on queens by our established RNAi system. Furthermore, no strict sequence-related off-target effects were detected. Finally, the RNAi effect of large-scale bacteria expressing dsRNA was successfully confirmed for controlling S. invicta. In summary, this study established an RNAi system for S. invicta and provided a research template for the future development of nucleic acid drugs based on RNAi.</description><identifier>ISSN: 0021-8561</identifier><identifier>ISSN: 1520-5118</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.4c00654</identifier><identifier>PMID: 38691835</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Bioactive Constituents, Metabolites, and Functions ; control methods ; food chemistry ; genes ; insects ; nucleic acids ; pest control ; Solenopsis invicta</subject><ispartof>Journal of agricultural and food chemistry, 2024-05, Vol.72 (19), p.10936-10943</ispartof><rights>2024 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a322t-6e3a94e336ff51c804e9bcfec564f645ce290ff3e967402c1d16bba0d4e662f73</cites><orcidid>0000-0003-0471-2964</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.4c00654$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.4c00654$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38691835$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Jin-da</creatorcontrib><creatorcontrib>Chen, Yao-hui</creatorcontrib><creatorcontrib>Zhang, Ya-xin</creatorcontrib><creatorcontrib>Lin, Jin-wen</creatorcontrib><creatorcontrib>Gao, San-ji</creatorcontrib><creatorcontrib>Tang, Bao-zhen</creatorcontrib><creatorcontrib>Hou, You-ming</creatorcontrib><title>Establishment of RNAi-Mediated Pest Control Method for Red Imported Fire Ant, Solenopsis invicta</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>RNAi plays a crucial role in insect gene function research and pest control field. Nonetheless, the variable efficiency of RNAi across diverse insects and off-target effects also limited its further application. In this study, we cloned six essential housekeeping genes from Solenopsis invicta and conducted RNAi experiments by orally administering dsRNA. Then, we found that mixing with liposomes significantly enhanced the RNAi efficiency by targeting for SiV-ATPaseE. Additionally, we observed a certain lethal effect of this dsRNA on queens by our established RNAi system. Furthermore, no strict sequence-related off-target effects were detected. Finally, the RNAi effect of large-scale bacteria expressing dsRNA was successfully confirmed for controlling S. invicta. In summary, this study established an RNAi system for S. invicta and provided a research template for the future development of nucleic acid drugs based on RNAi.</description><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>control methods</subject><subject>food chemistry</subject><subject>genes</subject><subject>insects</subject><subject>nucleic acids</subject><subject>pest control</subject><subject>Solenopsis invicta</subject><issn>0021-8561</issn><issn>1520-5118</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkT1v2zAQhomiQe0k3TsVHDtEzlH8MDUaRr6AOC2SdlYp6gjTkESXpAP030eOnW5BpxvueV8c7iHkC4MZg5JdGptmG-PsTFgAJcUHMmWyhEIypj-SKYxMoaViE3Ka0gYAtJzDJzLhWlVMczklv69SNk3n07rHIdPg6OPDwhcrbL3J2NIfmDJdhiHH0NEV5nVoqQuRPo67u34b4h669hHpYsgX9Cl0OIRt8on64dnbbM7JiTNdws_HeUZ-XV_9XN4W999v7paL-8LwssyFQm4qgZwr5ySzGgRWjXVopRJOCWmxrMA5jpWaCygta5lqGgOtQKVKN-dn5NuhdxvDn914dd37ZLHrzIBhl2rOJNei1Fz_HwUJbM5ZJUYUDqiNIaWIrt5G35v4t2ZQ7xXUo4J6r6A-KhgjX4_tu6bH9l_g7ecjcHEAXqNhF4fxL-_3vQBjK5GO</recordid><startdate>20240515</startdate><enddate>20240515</enddate><creator>Wang, Jin-da</creator><creator>Chen, Yao-hui</creator><creator>Zhang, Ya-xin</creator><creator>Lin, Jin-wen</creator><creator>Gao, San-ji</creator><creator>Tang, Bao-zhen</creator><creator>Hou, You-ming</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-0471-2964</orcidid></search><sort><creationdate>20240515</creationdate><title>Establishment of RNAi-Mediated Pest Control Method for Red Imported Fire Ant, Solenopsis invicta</title><author>Wang, Jin-da ; Chen, Yao-hui ; Zhang, Ya-xin ; Lin, Jin-wen ; Gao, San-ji ; Tang, Bao-zhen ; Hou, You-ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a322t-6e3a94e336ff51c804e9bcfec564f645ce290ff3e967402c1d16bba0d4e662f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>control methods</topic><topic>food chemistry</topic><topic>genes</topic><topic>insects</topic><topic>nucleic acids</topic><topic>pest control</topic><topic>Solenopsis invicta</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jin-da</creatorcontrib><creatorcontrib>Chen, Yao-hui</creatorcontrib><creatorcontrib>Zhang, Ya-xin</creatorcontrib><creatorcontrib>Lin, Jin-wen</creatorcontrib><creatorcontrib>Gao, San-ji</creatorcontrib><creatorcontrib>Tang, Bao-zhen</creatorcontrib><creatorcontrib>Hou, You-ming</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jin-da</au><au>Chen, Yao-hui</au><au>Zhang, Ya-xin</au><au>Lin, Jin-wen</au><au>Gao, San-ji</au><au>Tang, Bao-zhen</au><au>Hou, You-ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of RNAi-Mediated Pest Control Method for Red Imported Fire Ant, Solenopsis invicta</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2024-05-15</date><risdate>2024</risdate><volume>72</volume><issue>19</issue><spage>10936</spage><epage>10943</epage><pages>10936-10943</pages><issn>0021-8561</issn><issn>1520-5118</issn><eissn>1520-5118</eissn><abstract>RNAi plays a crucial role in insect gene function research and pest control field. Nonetheless, the variable efficiency of RNAi across diverse insects and off-target effects also limited its further application. In this study, we cloned six essential housekeeping genes from Solenopsis invicta and conducted RNAi experiments by orally administering dsRNA. Then, we found that mixing with liposomes significantly enhanced the RNAi efficiency by targeting for SiV-ATPaseE. Additionally, we observed a certain lethal effect of this dsRNA on queens by our established RNAi system. Furthermore, no strict sequence-related off-target effects were detected. Finally, the RNAi effect of large-scale bacteria expressing dsRNA was successfully confirmed for controlling S. invicta. In summary, this study established an RNAi system for S. invicta and provided a research template for the future development of nucleic acid drugs based on RNAi.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>38691835</pmid><doi>10.1021/acs.jafc.4c00654</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-0471-2964</orcidid></addata></record> |
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| source | American Chemical Society Journals |
| subjects | Bioactive Constituents, Metabolites, and Functions control methods food chemistry genes insects nucleic acids pest control Solenopsis invicta |
| title | Establishment of RNAi-Mediated Pest Control Method for Red Imported Fire Ant, Solenopsis invicta |
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