Genome Analysis of Multiple Polysaccharide-Degrading Bacterium Microbulbifer thermotolerans HB226069: Determination of Alginate Lyase Activity
Polysaccharide-degrading bacteria are key participants in the global carbon cycle and algal biomass recycling. Herein, a polysaccharide lyase-producing strain HB226069 was isolated from Sargassum sp. from Qingge Port, Hainan, China. Results of the phylogenetic of the 16S rRNA gene and genotypic anal...
Gespeichert in:
| Veröffentlicht in: | Marine biotechnology (New York, N.Y.) N.Y.), 2024-06, Vol.26 (3), p.488-499 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 499 |
|---|---|
| container_issue | 3 |
| container_start_page | 488 |
| container_title | Marine biotechnology (New York, N.Y.) |
| container_volume | 26 |
| creator | Li, Xue Yang, Miao Mo, Kunlian Hu, Yonghua Gu, Hanjie Sun, Dongmei Bao, Shixiang Huang, Huiqin |
| description | Polysaccharide-degrading bacteria are key participants in the global carbon cycle and algal biomass recycling. Herein, a polysaccharide lyase-producing strain HB226069 was isolated from
Sargassum
sp. from Qingge Port, Hainan, China. Results of the phylogenetic of the 16S rRNA gene and genotypic analysis indicated that the isolate should be classified as
Microbulbifer thermotolerans
. The whole genome is a 4,021,337 bp circular chromosome with a G+C content of 56.5%. Analysis of the predicted genes indicated that strain HB226069 encoded 161 carbohydrate-active enzymes (CAZymes), and abundant putative enzymes involved in polysaccharide degradation were predicted, including alginate lyase, fucosidase, agarase, xylanase, cellulase, pectate lyase, amylase, and chitinase. Three of the putative polysaccharide lyases from PL7 and PL17 families were involved in alginate degradation. The alginate lyases of strain HB226069 showed the maximum activity of 117.4 U/mL at 50 °C, pH 7.0, and 0.05 M FeCl
3
, while exhibiting the best stability at 30 °C and pH 7.0. The Thin Layer Chromatography (TLC) and Electrospray Ionization Mass Spectrometry (ESI-MS) analyses indicated that the alginate oligosaccharides (AOSs) degraded by the partially purified alginate lyases contained oligosaccharides of DP2–DP5 and monosaccharide while reacting for 36 h. The complete genome of
M. thermotolerans
HB226069 enriches our understanding of the mechanism of polysaccharide lyase production and supports its potential application in polysaccharide degradation. |
| doi_str_mv | 10.1007/s10126-024-10311-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3047953417</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3153645000</sourcerecordid><originalsourceid>FETCH-LOGICAL-c359t-97edc6d9b4614db7d66c564c310ed29e306e6370927b3814397ecc8dd7e75a483</originalsourceid><addsrcrecordid>eNqFkc1u1DAUhS0Eoj_wAiyQJTZsAv6LnbCbtrRFmgoWsLYc-87UlRMPtlNpXoJnxmFKkVjAyvb1d86VzkHoFSXvKCHqfaaEMtkQJhpKOKUNfYKOqeCyYYzLp4931h2hk5zvSBUpTp6jI95J2fVUHaMfVzDFEfBqMmGffcZxg2_mUPwuAP4S68xYe2uSd9BcwDYZ56ctPjO2QPLziG-8TXGYw-A3kHC5hTTGEgMkM2V8fcaYJLL_gC-g8qOfTPFxWnaswnZ5AV7vTa7rbfH3vuxfoGcbEzK8fDhP0bfLj1_Pr5v156tP56t1Y3nbl6ZX4Kx0_SAkFW5QTkrbSmE5JeBYD5xIkFyRnqmBdzWGKrC2c06Bao3o-Cl6e_Ddpfh9hlz06LOFEMwEcc6a05ZL0dbE_o8SofqWC6oq-uYv9C7OqSa7ULJjXAm2GLIDVZPLOcFG75IfTdprSvRSrD4Uq2ux-lexmlbR6wfreRjBPUp-N1kBfgBy_Zq2kP7s_oftT0lwrdI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3068237420</pqid></control><display><type>article</type><title>Genome Analysis of Multiple Polysaccharide-Degrading Bacterium Microbulbifer thermotolerans HB226069: Determination of Alginate Lyase Activity</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Li, Xue ; Yang, Miao ; Mo, Kunlian ; Hu, Yonghua ; Gu, Hanjie ; Sun, Dongmei ; Bao, Shixiang ; Huang, Huiqin</creator><creatorcontrib>Li, Xue ; Yang, Miao ; Mo, Kunlian ; Hu, Yonghua ; Gu, Hanjie ; Sun, Dongmei ; Bao, Shixiang ; Huang, Huiqin</creatorcontrib><description>Polysaccharide-degrading bacteria are key participants in the global carbon cycle and algal biomass recycling. Herein, a polysaccharide lyase-producing strain HB226069 was isolated from
Sargassum
sp. from Qingge Port, Hainan, China. Results of the phylogenetic of the 16S rRNA gene and genotypic analysis indicated that the isolate should be classified as
Microbulbifer thermotolerans
. The whole genome is a 4,021,337 bp circular chromosome with a G+C content of 56.5%. Analysis of the predicted genes indicated that strain HB226069 encoded 161 carbohydrate-active enzymes (CAZymes), and abundant putative enzymes involved in polysaccharide degradation were predicted, including alginate lyase, fucosidase, agarase, xylanase, cellulase, pectate lyase, amylase, and chitinase. Three of the putative polysaccharide lyases from PL7 and PL17 families were involved in alginate degradation. The alginate lyases of strain HB226069 showed the maximum activity of 117.4 U/mL at 50 °C, pH 7.0, and 0.05 M FeCl
3
, while exhibiting the best stability at 30 °C and pH 7.0. The Thin Layer Chromatography (TLC) and Electrospray Ionization Mass Spectrometry (ESI-MS) analyses indicated that the alginate oligosaccharides (AOSs) degraded by the partially purified alginate lyases contained oligosaccharides of DP2–DP5 and monosaccharide while reacting for 36 h. The complete genome of
M. thermotolerans
HB226069 enriches our understanding of the mechanism of polysaccharide lyase production and supports its potential application in polysaccharide degradation.</description><identifier>ISSN: 1436-2228</identifier><identifier>ISSN: 1436-2236</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s10126-024-10311-1</identifier><identifier>PMID: 38668917</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Agarase ; Algae ; Alginate lyase ; Alginates ; Alginates - metabolism ; Alginic acid ; amylases ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteroidetes - enzymology ; Bacteroidetes - genetics ; Bacteroidetes - metabolism ; Base Composition ; Biodegradation ; biomass ; Biomedical and Life Sciences ; Carbohydrates ; Carbon cycle ; Cellulase ; China ; Chitinase ; Chromatography ; Chromosomes ; Degradation ; electrospray ionization mass spectrometry ; endo-1,4-beta-glucanase ; Engineering ; Enzymes ; Ferric chloride ; Freshwater & Marine Ecology ; genes ; Genome, Bacterial ; Genomes ; Genomic analysis ; global carbon budget ; Ionization ; Life Sciences ; Lyases ; Mass spectrometry ; Mass spectroscopy ; Microbiology ; Microbulbifer ; Monosaccharides ; Oligosaccharides ; Pectate lyase ; Phylogenetics ; Phylogeny ; Polysaccharide-Lyases - genetics ; Polysaccharide-Lyases - metabolism ; Polysaccharides ; Polysaccharides - metabolism ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sargassum ; Sargassum - metabolism ; Sargassum - microbiology ; Seaweed meal ; sequence analysis ; Thin layer chromatography ; Xylanase ; xylanases ; Zoology</subject><ispartof>Marine biotechnology (New York, N.Y.), 2024-06, Vol.26 (3), p.488-499</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-97edc6d9b4614db7d66c564c310ed29e306e6370927b3814397ecc8dd7e75a483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10126-024-10311-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10126-024-10311-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38668917$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xue</creatorcontrib><creatorcontrib>Yang, Miao</creatorcontrib><creatorcontrib>Mo, Kunlian</creatorcontrib><creatorcontrib>Hu, Yonghua</creatorcontrib><creatorcontrib>Gu, Hanjie</creatorcontrib><creatorcontrib>Sun, Dongmei</creatorcontrib><creatorcontrib>Bao, Shixiang</creatorcontrib><creatorcontrib>Huang, Huiqin</creatorcontrib><title>Genome Analysis of Multiple Polysaccharide-Degrading Bacterium Microbulbifer thermotolerans HB226069: Determination of Alginate Lyase Activity</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol</addtitle><addtitle>Mar Biotechnol (NY)</addtitle><description>Polysaccharide-degrading bacteria are key participants in the global carbon cycle and algal biomass recycling. Herein, a polysaccharide lyase-producing strain HB226069 was isolated from
Sargassum
sp. from Qingge Port, Hainan, China. Results of the phylogenetic of the 16S rRNA gene and genotypic analysis indicated that the isolate should be classified as
Microbulbifer thermotolerans
. The whole genome is a 4,021,337 bp circular chromosome with a G+C content of 56.5%. Analysis of the predicted genes indicated that strain HB226069 encoded 161 carbohydrate-active enzymes (CAZymes), and abundant putative enzymes involved in polysaccharide degradation were predicted, including alginate lyase, fucosidase, agarase, xylanase, cellulase, pectate lyase, amylase, and chitinase. Three of the putative polysaccharide lyases from PL7 and PL17 families were involved in alginate degradation. The alginate lyases of strain HB226069 showed the maximum activity of 117.4 U/mL at 50 °C, pH 7.0, and 0.05 M FeCl
3
, while exhibiting the best stability at 30 °C and pH 7.0. The Thin Layer Chromatography (TLC) and Electrospray Ionization Mass Spectrometry (ESI-MS) analyses indicated that the alginate oligosaccharides (AOSs) degraded by the partially purified alginate lyases contained oligosaccharides of DP2–DP5 and monosaccharide while reacting for 36 h. The complete genome of
M. thermotolerans
HB226069 enriches our understanding of the mechanism of polysaccharide lyase production and supports its potential application in polysaccharide degradation.</description><subject>Agarase</subject><subject>Algae</subject><subject>Alginate lyase</subject><subject>Alginates</subject><subject>Alginates - metabolism</subject><subject>Alginic acid</subject><subject>amylases</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteroidetes - enzymology</subject><subject>Bacteroidetes - genetics</subject><subject>Bacteroidetes - metabolism</subject><subject>Base Composition</subject><subject>Biodegradation</subject><subject>biomass</subject><subject>Biomedical and Life Sciences</subject><subject>Carbohydrates</subject><subject>Carbon cycle</subject><subject>Cellulase</subject><subject>China</subject><subject>Chitinase</subject><subject>Chromatography</subject><subject>Chromosomes</subject><subject>Degradation</subject><subject>electrospray ionization mass spectrometry</subject><subject>endo-1,4-beta-glucanase</subject><subject>Engineering</subject><subject>Enzymes</subject><subject>Ferric chloride</subject><subject>Freshwater & Marine Ecology</subject><subject>genes</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Genomic analysis</subject><subject>global carbon budget</subject><subject>Ionization</subject><subject>Life Sciences</subject><subject>Lyases</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Microbiology</subject><subject>Microbulbifer</subject><subject>Monosaccharides</subject><subject>Oligosaccharides</subject><subject>Pectate lyase</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polysaccharide-Lyases - genetics</subject><subject>Polysaccharide-Lyases - metabolism</subject><subject>Polysaccharides</subject><subject>Polysaccharides - metabolism</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Sargassum</subject><subject>Sargassum - metabolism</subject><subject>Sargassum - microbiology</subject><subject>Seaweed meal</subject><subject>sequence analysis</subject><subject>Thin layer chromatography</subject><subject>Xylanase</subject><subject>xylanases</subject><subject>Zoology</subject><issn>1436-2228</issn><issn>1436-2236</issn><issn>1436-2236</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0Eoj_wAiyQJTZsAv6LnbCbtrRFmgoWsLYc-87UlRMPtlNpXoJnxmFKkVjAyvb1d86VzkHoFSXvKCHqfaaEMtkQJhpKOKUNfYKOqeCyYYzLp4931h2hk5zvSBUpTp6jI95J2fVUHaMfVzDFEfBqMmGffcZxg2_mUPwuAP4S68xYe2uSd9BcwDYZ56ctPjO2QPLziG-8TXGYw-A3kHC5hTTGEgMkM2V8fcaYJLL_gC-g8qOfTPFxWnaswnZ5AV7vTa7rbfH3vuxfoGcbEzK8fDhP0bfLj1_Pr5v156tP56t1Y3nbl6ZX4Kx0_SAkFW5QTkrbSmE5JeBYD5xIkFyRnqmBdzWGKrC2c06Bao3o-Cl6e_Ddpfh9hlz06LOFEMwEcc6a05ZL0dbE_o8SofqWC6oq-uYv9C7OqSa7ULJjXAm2GLIDVZPLOcFG75IfTdprSvRSrD4Uq2ux-lexmlbR6wfreRjBPUp-N1kBfgBy_Zq2kP7s_oftT0lwrdI</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Li, Xue</creator><creator>Yang, Miao</creator><creator>Mo, Kunlian</creator><creator>Hu, Yonghua</creator><creator>Gu, Hanjie</creator><creator>Sun, Dongmei</creator><creator>Bao, Shixiang</creator><creator>Huang, Huiqin</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TN</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>K9.</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240601</creationdate><title>Genome Analysis of Multiple Polysaccharide-Degrading Bacterium Microbulbifer thermotolerans HB226069: Determination of Alginate Lyase Activity</title><author>Li, Xue ; Yang, Miao ; Mo, Kunlian ; Hu, Yonghua ; Gu, Hanjie ; Sun, Dongmei ; Bao, Shixiang ; Huang, Huiqin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-97edc6d9b4614db7d66c564c310ed29e306e6370927b3814397ecc8dd7e75a483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Agarase</topic><topic>Algae</topic><topic>Alginate lyase</topic><topic>Alginates</topic><topic>Alginates - metabolism</topic><topic>Alginic acid</topic><topic>amylases</topic><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteroidetes - enzymology</topic><topic>Bacteroidetes - genetics</topic><topic>Bacteroidetes - metabolism</topic><topic>Base Composition</topic><topic>Biodegradation</topic><topic>biomass</topic><topic>Biomedical and Life Sciences</topic><topic>Carbohydrates</topic><topic>Carbon cycle</topic><topic>Cellulase</topic><topic>China</topic><topic>Chitinase</topic><topic>Chromatography</topic><topic>Chromosomes</topic><topic>Degradation</topic><topic>electrospray ionization mass spectrometry</topic><topic>endo-1,4-beta-glucanase</topic><topic>Engineering</topic><topic>Enzymes</topic><topic>Ferric chloride</topic><topic>Freshwater & Marine Ecology</topic><topic>genes</topic><topic>Genome, Bacterial</topic><topic>Genomes</topic><topic>Genomic analysis</topic><topic>global carbon budget</topic><topic>Ionization</topic><topic>Life Sciences</topic><topic>Lyases</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Microbiology</topic><topic>Microbulbifer</topic><topic>Monosaccharides</topic><topic>Oligosaccharides</topic><topic>Pectate lyase</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Polysaccharide-Lyases - genetics</topic><topic>Polysaccharide-Lyases - metabolism</topic><topic>Polysaccharides</topic><topic>Polysaccharides - metabolism</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Sargassum</topic><topic>Sargassum - metabolism</topic><topic>Sargassum - microbiology</topic><topic>Seaweed meal</topic><topic>sequence analysis</topic><topic>Thin layer chromatography</topic><topic>Xylanase</topic><topic>xylanases</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xue</creatorcontrib><creatorcontrib>Yang, Miao</creatorcontrib><creatorcontrib>Mo, Kunlian</creatorcontrib><creatorcontrib>Hu, Yonghua</creatorcontrib><creatorcontrib>Gu, Hanjie</creatorcontrib><creatorcontrib>Sun, Dongmei</creatorcontrib><creatorcontrib>Bao, Shixiang</creatorcontrib><creatorcontrib>Huang, Huiqin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Marine biotechnology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xue</au><au>Yang, Miao</au><au>Mo, Kunlian</au><au>Hu, Yonghua</au><au>Gu, Hanjie</au><au>Sun, Dongmei</au><au>Bao, Shixiang</au><au>Huang, Huiqin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome Analysis of Multiple Polysaccharide-Degrading Bacterium Microbulbifer thermotolerans HB226069: Determination of Alginate Lyase Activity</atitle><jtitle>Marine biotechnology (New York, N.Y.)</jtitle><stitle>Mar Biotechnol</stitle><addtitle>Mar Biotechnol (NY)</addtitle><date>2024-06-01</date><risdate>2024</risdate><volume>26</volume><issue>3</issue><spage>488</spage><epage>499</epage><pages>488-499</pages><issn>1436-2228</issn><issn>1436-2236</issn><eissn>1436-2236</eissn><abstract>Polysaccharide-degrading bacteria are key participants in the global carbon cycle and algal biomass recycling. Herein, a polysaccharide lyase-producing strain HB226069 was isolated from
Sargassum
sp. from Qingge Port, Hainan, China. Results of the phylogenetic of the 16S rRNA gene and genotypic analysis indicated that the isolate should be classified as
Microbulbifer thermotolerans
. The whole genome is a 4,021,337 bp circular chromosome with a G+C content of 56.5%. Analysis of the predicted genes indicated that strain HB226069 encoded 161 carbohydrate-active enzymes (CAZymes), and abundant putative enzymes involved in polysaccharide degradation were predicted, including alginate lyase, fucosidase, agarase, xylanase, cellulase, pectate lyase, amylase, and chitinase. Three of the putative polysaccharide lyases from PL7 and PL17 families were involved in alginate degradation. The alginate lyases of strain HB226069 showed the maximum activity of 117.4 U/mL at 50 °C, pH 7.0, and 0.05 M FeCl
3
, while exhibiting the best stability at 30 °C and pH 7.0. The Thin Layer Chromatography (TLC) and Electrospray Ionization Mass Spectrometry (ESI-MS) analyses indicated that the alginate oligosaccharides (AOSs) degraded by the partially purified alginate lyases contained oligosaccharides of DP2–DP5 and monosaccharide while reacting for 36 h. The complete genome of
M. thermotolerans
HB226069 enriches our understanding of the mechanism of polysaccharide lyase production and supports its potential application in polysaccharide degradation.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>38668917</pmid><doi>10.1007/s10126-024-10311-1</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1436-2228 |
| ispartof | Marine biotechnology (New York, N.Y.), 2024-06, Vol.26 (3), p.488-499 |
| issn | 1436-2228 1436-2236 1436-2236 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3047953417 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Agarase Algae Alginate lyase Alginates Alginates - metabolism Alginic acid amylases Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteroidetes - enzymology Bacteroidetes - genetics Bacteroidetes - metabolism Base Composition Biodegradation biomass Biomedical and Life Sciences Carbohydrates Carbon cycle Cellulase China Chitinase Chromatography Chromosomes Degradation electrospray ionization mass spectrometry endo-1,4-beta-glucanase Engineering Enzymes Ferric chloride Freshwater & Marine Ecology genes Genome, Bacterial Genomes Genomic analysis global carbon budget Ionization Life Sciences Lyases Mass spectrometry Mass spectroscopy Microbiology Microbulbifer Monosaccharides Oligosaccharides Pectate lyase Phylogenetics Phylogeny Polysaccharide-Lyases - genetics Polysaccharide-Lyases - metabolism Polysaccharides Polysaccharides - metabolism RNA, Ribosomal, 16S - genetics rRNA 16S Sargassum Sargassum - metabolism Sargassum - microbiology Seaweed meal sequence analysis Thin layer chromatography Xylanase xylanases Zoology |
| title | Genome Analysis of Multiple Polysaccharide-Degrading Bacterium Microbulbifer thermotolerans HB226069: Determination of Alginate Lyase Activity |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T01%3A38%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genome%20Analysis%20of%20Multiple%20Polysaccharide-Degrading%20Bacterium%20Microbulbifer%20thermotolerans%20HB226069:%20Determination%20of%20Alginate%20Lyase%20Activity&rft.jtitle=Marine%20biotechnology%20(New%20York,%20N.Y.)&rft.au=Li,%20Xue&rft.date=2024-06-01&rft.volume=26&rft.issue=3&rft.spage=488&rft.epage=499&rft.pages=488-499&rft.issn=1436-2228&rft.eissn=1436-2236&rft_id=info:doi/10.1007/s10126-024-10311-1&rft_dat=%3Cproquest_cross%3E3153645000%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3068237420&rft_id=info:pmid/38668917&rfr_iscdi=true |