Microalgae extracts modulates the immune response in Trypanosoma cruzi-infected human cells
[Display omitted] •Chlorella vulgaris and Tetradesmus obliquus presents trypanocidal activity.•T. obliquus displayed the highest selectivity index against T. cruzi.•C. vulgaris and T. obliquus presents immunomodulatory effects.•C. vulgaris decreased inflammation by the reducing of the levels of IFN....
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| Veröffentlicht in: | Cytokine (Philadelphia, Pa.) Pa.), 2024-07, Vol.179, p.156621-156621, Article 156621 |
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| creator | da Silva Júnior, José Noé Oliveira, Kamila Kassia dos Santos Silva, Ana Carla da Lorena, Virginia Maria Barros de Marques, Daniela de Araújo Viana Bezerra, Raquel Pedrosa Porto, Ana Lúcia Figueiredo |
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•Chlorella vulgaris and Tetradesmus obliquus presents trypanocidal activity.•T. obliquus displayed the highest selectivity index against T. cruzi.•C. vulgaris and T. obliquus presents immunomodulatory effects.•C. vulgaris decreased inflammation by the reducing of the levels of IFN.•T. obliquus modulates the immune response, increasing the levels of TNF and IL-10.
Chagas disease (CD) is caused by the hemoflagellate protozoan Trypanosoma cruzi. The control of the infection depends of the innate and acquired immune response of host. Moreover, CD plays a significant role in the immune response, and, in this context, microalgae can be an interesting alternative due to its immunomodulatory and trypanocidal effects. This study aimed to evaluate, in vitro, immunomodulatory potentials of the aqueous extracts of Chlorella vulgaris and Tetradesmus obliquus. Both microalgae extracts (ME) were obtained by sonication, and the selectivity index (SI) was determined by assays of inhibitory concentration (IC50) in T. cruzi trypomastigotes cells; as well as the cytotoxic concentrations (CC50) in human peripheral mononuclear cells (PBMC). The immune response was evaluated in T. cruzi-infected PBMC using the IC50 value. ME led to inhibition of T. cruzi trypomastigotes after 24 h of treatment, in which the IC50 values were 112.1 µg/ml to C. vulgaris and 15.8 µg ml−1 to T. obliquus. On the other hand, C. vulgaris did not affect the viability of PBMCs in concentrations up to 1000 µg ml−1, while T. obliquus was non-toxic to PBMCs in concentrations up to 253.44 µg ml−1. In addition, T. obliquus displayed a higher SI against T. cruzi (SI = 16.8), when compared with C. vulgaris (SI = 8.9). C. vulgaris decreased the levels of IFN, indicating a reduction of the inflammatory process; while T. obliquus displayed an interesting immunomodulatory effect, since discretely increased the levels of TNF and stimulated the production of the anti-inflammatory cytokine IL-10. This study confirms that ME are effective against T. cruzi trypomastigotes, and may able to control the parasitemia and preventing the progress of CD while regulating the inflammatory process. |
| doi_str_mv | 10.1016/j.cyto.2024.156621 |
| format | Article |
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•Chlorella vulgaris and Tetradesmus obliquus presents trypanocidal activity.•T. obliquus displayed the highest selectivity index against T. cruzi.•C. vulgaris and T. obliquus presents immunomodulatory effects.•C. vulgaris decreased inflammation by the reducing of the levels of IFN.•T. obliquus modulates the immune response, increasing the levels of TNF and IL-10.
Chagas disease (CD) is caused by the hemoflagellate protozoan Trypanosoma cruzi. The control of the infection depends of the innate and acquired immune response of host. Moreover, CD plays a significant role in the immune response, and, in this context, microalgae can be an interesting alternative due to its immunomodulatory and trypanocidal effects. This study aimed to evaluate, in vitro, immunomodulatory potentials of the aqueous extracts of Chlorella vulgaris and Tetradesmus obliquus. Both microalgae extracts (ME) were obtained by sonication, and the selectivity index (SI) was determined by assays of inhibitory concentration (IC50) in T. cruzi trypomastigotes cells; as well as the cytotoxic concentrations (CC50) in human peripheral mononuclear cells (PBMC). The immune response was evaluated in T. cruzi-infected PBMC using the IC50 value. ME led to inhibition of T. cruzi trypomastigotes after 24 h of treatment, in which the IC50 values were 112.1 µg/ml to C. vulgaris and 15.8 µg ml−1 to T. obliquus. On the other hand, C. vulgaris did not affect the viability of PBMCs in concentrations up to 1000 µg ml−1, while T. obliquus was non-toxic to PBMCs in concentrations up to 253.44 µg ml−1. In addition, T. obliquus displayed a higher SI against T. cruzi (SI = 16.8), when compared with C. vulgaris (SI = 8.9). C. vulgaris decreased the levels of IFN, indicating a reduction of the inflammatory process; while T. obliquus displayed an interesting immunomodulatory effect, since discretely increased the levels of TNF and stimulated the production of the anti-inflammatory cytokine IL-10. This study confirms that ME are effective against T. cruzi trypomastigotes, and may able to control the parasitemia and preventing the progress of CD while regulating the inflammatory process.</description><identifier>ISSN: 1043-4666</identifier><identifier>EISSN: 1096-0023</identifier><identifier>DOI: 10.1016/j.cyto.2024.156621</identifier><identifier>PMID: 38648682</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Chagas disease ; Chagas Disease - drug therapy ; Chagas Disease - immunology ; Chagas Disease - parasitology ; Cytokines ; Cytokines - metabolism ; Humans ; Inflammation ; Leukocytes, Mononuclear - drug effects ; Leukocytes, Mononuclear - immunology ; Leukocytes, Mononuclear - metabolism ; Microalgae - chemistry ; Microalgae extracts ; Plant Extracts - pharmacology ; Trypanosoma cruzi - drug effects ; Trypanosoma cruzi - immunology</subject><ispartof>Cytokine (Philadelphia, Pa.), 2024-07, Vol.179, p.156621-156621, Article 156621</ispartof><rights>2024</rights><rights>Copyright © 2024. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c307t-620a0496d551d1f1b572a2cc0be67565d2102ff8e75cf4c00db75a805b4e194c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1043466624001248$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38648682$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Silva Júnior, José Noé</creatorcontrib><creatorcontrib>Oliveira, Kamila Kassia dos Santos</creatorcontrib><creatorcontrib>Silva, Ana Carla da</creatorcontrib><creatorcontrib>Lorena, Virginia Maria Barros de</creatorcontrib><creatorcontrib>Marques, Daniela de Araújo Viana</creatorcontrib><creatorcontrib>Bezerra, Raquel Pedrosa</creatorcontrib><creatorcontrib>Porto, Ana Lúcia Figueiredo</creatorcontrib><title>Microalgae extracts modulates the immune response in Trypanosoma cruzi-infected human cells</title><title>Cytokine (Philadelphia, Pa.)</title><addtitle>Cytokine</addtitle><description>[Display omitted]
•Chlorella vulgaris and Tetradesmus obliquus presents trypanocidal activity.•T. obliquus displayed the highest selectivity index against T. cruzi.•C. vulgaris and T. obliquus presents immunomodulatory effects.•C. vulgaris decreased inflammation by the reducing of the levels of IFN.•T. obliquus modulates the immune response, increasing the levels of TNF and IL-10.
Chagas disease (CD) is caused by the hemoflagellate protozoan Trypanosoma cruzi. The control of the infection depends of the innate and acquired immune response of host. Moreover, CD plays a significant role in the immune response, and, in this context, microalgae can be an interesting alternative due to its immunomodulatory and trypanocidal effects. This study aimed to evaluate, in vitro, immunomodulatory potentials of the aqueous extracts of Chlorella vulgaris and Tetradesmus obliquus. Both microalgae extracts (ME) were obtained by sonication, and the selectivity index (SI) was determined by assays of inhibitory concentration (IC50) in T. cruzi trypomastigotes cells; as well as the cytotoxic concentrations (CC50) in human peripheral mononuclear cells (PBMC). The immune response was evaluated in T. cruzi-infected PBMC using the IC50 value. ME led to inhibition of T. cruzi trypomastigotes after 24 h of treatment, in which the IC50 values were 112.1 µg/ml to C. vulgaris and 15.8 µg ml−1 to T. obliquus. On the other hand, C. vulgaris did not affect the viability of PBMCs in concentrations up to 1000 µg ml−1, while T. obliquus was non-toxic to PBMCs in concentrations up to 253.44 µg ml−1. In addition, T. obliquus displayed a higher SI against T. cruzi (SI = 16.8), when compared with C. vulgaris (SI = 8.9). C. vulgaris decreased the levels of IFN, indicating a reduction of the inflammatory process; while T. obliquus displayed an interesting immunomodulatory effect, since discretely increased the levels of TNF and stimulated the production of the anti-inflammatory cytokine IL-10. This study confirms that ME are effective against T. cruzi trypomastigotes, and may able to control the parasitemia and preventing the progress of CD while regulating the inflammatory process.</description><subject>Chagas disease</subject><subject>Chagas Disease - drug therapy</subject><subject>Chagas Disease - immunology</subject><subject>Chagas Disease - parasitology</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Microalgae - chemistry</subject><subject>Microalgae extracts</subject><subject>Plant Extracts - pharmacology</subject><subject>Trypanosoma cruzi - drug effects</subject><subject>Trypanosoma cruzi - immunology</subject><issn>1043-4666</issn><issn>1096-0023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtPxDAQhC0E4nHwByiQS5oca8d2chINQrwkEA1UFJZjb8CnJD7sBHH8ehIdUFLtrjQz2vkIOWYwZ8DU2XJu132Yc-BizqRSnG2RfQYLlQHwfHvaRZ4JpdQeOUhpCQCLvCh2yV5eKlGqku-TlwdvYzDNq0GKn300tk-0DW5oTI-J9m9IfdsOHdKIaRW6NN4dfYrrlelCCq2hNg5fPvNdjbZHR9-G1nTUYtOkQ7JTmybh0c-ckefrq6fL2-z-8ebu8uI-szkUfaY4GBAL5aRkjtWskgU33FqoUBVSSccZ8LousZC2FhbAVYU0JchKIFsIm8_I6SZ3FcP7gKnXrU_TB6bDMCSdg5CMKSHVKOUb6Vg6pYi1XkXfmrjWDPQEVS_1BFVPUPUG6mg6-ckfqhbdn-WX4ig43whwbPnhMepkPXYWnY8jFe2C_y__G2bLiWY</recordid><startdate>202407</startdate><enddate>202407</enddate><creator>da Silva Júnior, José Noé</creator><creator>Oliveira, Kamila Kassia dos Santos</creator><creator>Silva, Ana Carla da</creator><creator>Lorena, Virginia Maria Barros de</creator><creator>Marques, Daniela de Araújo Viana</creator><creator>Bezerra, Raquel Pedrosa</creator><creator>Porto, Ana Lúcia Figueiredo</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202407</creationdate><title>Microalgae extracts modulates the immune response in Trypanosoma cruzi-infected human cells</title><author>da Silva Júnior, José Noé ; Oliveira, Kamila Kassia dos Santos ; Silva, Ana Carla da ; Lorena, Virginia Maria Barros de ; Marques, Daniela de Araújo Viana ; Bezerra, Raquel Pedrosa ; Porto, Ana Lúcia Figueiredo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c307t-620a0496d551d1f1b572a2cc0be67565d2102ff8e75cf4c00db75a805b4e194c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Chagas disease</topic><topic>Chagas Disease - drug therapy</topic><topic>Chagas Disease - immunology</topic><topic>Chagas Disease - parasitology</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Microalgae - chemistry</topic><topic>Microalgae extracts</topic><topic>Plant Extracts - pharmacology</topic><topic>Trypanosoma cruzi - drug effects</topic><topic>Trypanosoma cruzi - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>da Silva Júnior, José Noé</creatorcontrib><creatorcontrib>Oliveira, Kamila Kassia dos Santos</creatorcontrib><creatorcontrib>Silva, Ana Carla da</creatorcontrib><creatorcontrib>Lorena, Virginia Maria Barros de</creatorcontrib><creatorcontrib>Marques, Daniela de Araújo Viana</creatorcontrib><creatorcontrib>Bezerra, Raquel Pedrosa</creatorcontrib><creatorcontrib>Porto, Ana Lúcia Figueiredo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cytokine (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Silva Júnior, José Noé</au><au>Oliveira, Kamila Kassia dos Santos</au><au>Silva, Ana Carla da</au><au>Lorena, Virginia Maria Barros de</au><au>Marques, Daniela de Araújo Viana</au><au>Bezerra, Raquel Pedrosa</au><au>Porto, Ana Lúcia Figueiredo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microalgae extracts modulates the immune response in Trypanosoma cruzi-infected human cells</atitle><jtitle>Cytokine (Philadelphia, Pa.)</jtitle><addtitle>Cytokine</addtitle><date>2024-07</date><risdate>2024</risdate><volume>179</volume><spage>156621</spage><epage>156621</epage><pages>156621-156621</pages><artnum>156621</artnum><issn>1043-4666</issn><eissn>1096-0023</eissn><abstract>[Display omitted]
•Chlorella vulgaris and Tetradesmus obliquus presents trypanocidal activity.•T. obliquus displayed the highest selectivity index against T. cruzi.•C. vulgaris and T. obliquus presents immunomodulatory effects.•C. vulgaris decreased inflammation by the reducing of the levels of IFN.•T. obliquus modulates the immune response, increasing the levels of TNF and IL-10.
Chagas disease (CD) is caused by the hemoflagellate protozoan Trypanosoma cruzi. The control of the infection depends of the innate and acquired immune response of host. Moreover, CD plays a significant role in the immune response, and, in this context, microalgae can be an interesting alternative due to its immunomodulatory and trypanocidal effects. This study aimed to evaluate, in vitro, immunomodulatory potentials of the aqueous extracts of Chlorella vulgaris and Tetradesmus obliquus. Both microalgae extracts (ME) were obtained by sonication, and the selectivity index (SI) was determined by assays of inhibitory concentration (IC50) in T. cruzi trypomastigotes cells; as well as the cytotoxic concentrations (CC50) in human peripheral mononuclear cells (PBMC). The immune response was evaluated in T. cruzi-infected PBMC using the IC50 value. ME led to inhibition of T. cruzi trypomastigotes after 24 h of treatment, in which the IC50 values were 112.1 µg/ml to C. vulgaris and 15.8 µg ml−1 to T. obliquus. On the other hand, C. vulgaris did not affect the viability of PBMCs in concentrations up to 1000 µg ml−1, while T. obliquus was non-toxic to PBMCs in concentrations up to 253.44 µg ml−1. In addition, T. obliquus displayed a higher SI against T. cruzi (SI = 16.8), when compared with C. vulgaris (SI = 8.9). C. vulgaris decreased the levels of IFN, indicating a reduction of the inflammatory process; while T. obliquus displayed an interesting immunomodulatory effect, since discretely increased the levels of TNF and stimulated the production of the anti-inflammatory cytokine IL-10. This study confirms that ME are effective against T. cruzi trypomastigotes, and may able to control the parasitemia and preventing the progress of CD while regulating the inflammatory process.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>38648682</pmid><doi>10.1016/j.cyto.2024.156621</doi><tpages>1</tpages></addata></record> |
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| subjects | Chagas disease Chagas Disease - drug therapy Chagas Disease - immunology Chagas Disease - parasitology Cytokines Cytokines - metabolism Humans Inflammation Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - metabolism Microalgae - chemistry Microalgae extracts Plant Extracts - pharmacology Trypanosoma cruzi - drug effects Trypanosoma cruzi - immunology |
| title | Microalgae extracts modulates the immune response in Trypanosoma cruzi-infected human cells |
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