Simple, sensitive, and visual detection of 12 respiratory pathogens with one‐pot‐RPA‐CRISPR/Cas12a assay

Respiratory infections pose a serious threat to global public health, underscoring the urgent need for rapid, accurate, and large‐scale diagnostic tools. In recent years, the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR‐associated) system, combined with isothermal amp...

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Veröffentlicht in:	Journal of medical virology 2024-04, Vol.96 (4), p.e29624-n/a
Hauptverfasser:	Tan, Qi, Shi, Yaoqiang, Duan, Chenlu, Li, Qingyuan, Gong, Tao, Li, Shilin, Duan, Xiaoqiong, Xie, He, Li, Yujia, Chen, Limin
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Bacteria Bacteria - genetics Bacteria - isolation & purification Bacterial Infections - diagnosis Bacterial Infections - microbiology Clinical isolates CRISPR CRISPR-Cas Systems CRISPR/Cas12a Diagnostic software Humans Molecular Diagnostic Techniques - methods Nucleic Acid Amplification Techniques - methods Nucleic acids Pathogens Plasmids point‐of‐care testing Precision medicine Public health Reagents Recombinase recombinase polymerase amplification (RPA) Recombinases - genetics Recombinases - metabolism Respiratory diseases respiratory pathogens Respiratory tract infection Respiratory Tract Infections - diagnosis Respiratory Tract Infections - microbiology Respiratory Tract Infections - virology Sensitivity and Specificity Viral infections Virus Diseases - diagnosis Viruses Viruses - genetics Viruses - isolation & purification
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