MicroRNA profiling of royal jelly extracellular vesicles and their potential role in cell viability and reversing cell apoptosis
MiRNAs are small non-coding RNA molecules that play important regulatory roles in diverse biological processes. Royal jelly, a milky-white substance produced by nurse honeybees ( Apis mellifera ), is the primary food of queen bees and plays a crucial role in their development. However, little is kno...
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| Veröffentlicht in: | Functional & integrative genomics 2023-09, Vol.23 (3), p.200-200, Article 200 |
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| creator | Saadeldin, Islam M. Tanga, Bereket Molla Bang, Seonggyu Maigoro, Abdulkadir Y. Kang, Heejae Cha, Dabin Lee, Soojin Lee, Sanghoon Cho, Jongki |
| description | MiRNAs are small non-coding RNA molecules that play important regulatory roles in diverse biological processes. Royal jelly, a milky-white substance produced by nurse honeybees (
Apis mellifera
), is the primary food of queen bees and plays a crucial role in their development. However, little is known about the microRNA (miRNAs) content of royal jelly and their potential functions. In this study, we isolated extracellular vesicles from the royal jelly of 36 samples through sequential centrifugation and targeted nanofiltration and performed high-throughput sequencing to identify and quantify the miRNA content of honeybee royal jelly extracellular vesicles (RJEVs). We found a total of 29 known mature miRNAs and 17 novel miRNAs. Through bioinformatic analysis, we identified several potential target genes of the miRNAs present in royal jelly, including those involved in developmental processes and cell differentiation. To investigate the potential roles of RJEVs in cell viability, RJEVs were supplemented to apoptotic porcine kidney fibroblasts induced by ethanol 6% exposure for 30 min. TUNEL assay showed a significant reduction in the apoptosis percentage after RJEV supplementation when compared with the non-supplemented control group. Moreover, the wound healing assay performed on the apoptotic cells showed a rapid healing capacity of RJEV-supplemented cells compared to the control group. We observed a significant reduction in the expression of the miRNA target genes such as FAM131B, ZEB1, COL5A1, TRIB2, YBX3, MAP2, CTNNA1, and ADAMTS9 suggesting that RJEVs may regulate the target gene expression associated with cellular motility and cell viability. Moreover, RJEVs reduced the expression of apoptotic genes (CASP3, TP53, BAX, and BAK), while significantly increasing the expression of anti-apoptotic genes (BCL2 and BCL-XL). Our findings provide the first comprehensive analysis of the miRNA content of RJEVs and suggest a potential role for these vesicles in the regulation of gene expression and cell survival as well as augmenting cell resurrection or anastasis. |
| doi_str_mv | 10.1007/s10142-023-01126-9 |
| format | Article |
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Apis mellifera
), is the primary food of queen bees and plays a crucial role in their development. However, little is known about the microRNA (miRNAs) content of royal jelly and their potential functions. In this study, we isolated extracellular vesicles from the royal jelly of 36 samples through sequential centrifugation and targeted nanofiltration and performed high-throughput sequencing to identify and quantify the miRNA content of honeybee royal jelly extracellular vesicles (RJEVs). We found a total of 29 known mature miRNAs and 17 novel miRNAs. Through bioinformatic analysis, we identified several potential target genes of the miRNAs present in royal jelly, including those involved in developmental processes and cell differentiation. To investigate the potential roles of RJEVs in cell viability, RJEVs were supplemented to apoptotic porcine kidney fibroblasts induced by ethanol 6% exposure for 30 min. TUNEL assay showed a significant reduction in the apoptosis percentage after RJEV supplementation when compared with the non-supplemented control group. Moreover, the wound healing assay performed on the apoptotic cells showed a rapid healing capacity of RJEV-supplemented cells compared to the control group. We observed a significant reduction in the expression of the miRNA target genes such as FAM131B, ZEB1, COL5A1, TRIB2, YBX3, MAP2, CTNNA1, and ADAMTS9 suggesting that RJEVs may regulate the target gene expression associated with cellular motility and cell viability. Moreover, RJEVs reduced the expression of apoptotic genes (CASP3, TP53, BAX, and BAK), while significantly increasing the expression of anti-apoptotic genes (BCL2 and BCL-XL). Our findings provide the first comprehensive analysis of the miRNA content of RJEVs and suggest a potential role for these vesicles in the regulation of gene expression and cell survival as well as augmenting cell resurrection or anastasis.</description><identifier>ISSN: 1438-793X</identifier><identifier>EISSN: 1438-7948</identifier><identifier>DOI: 10.1007/s10142-023-01126-9</identifier><identifier>PMID: 37284890</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animal Genetics and Genomics ; Apis mellifera ; Apoptosis ; Bcl-x protein ; Bees ; Biochemistry ; Bioinformatics ; Biomedical and Life Sciences ; Cell Biology ; Cell differentiation ; cell movement ; Cell survival ; Cell viability ; Centrifugation ; Epigenetics ; ethanol ; Extracellular vesicles ; Fibroblasts ; Gene expression ; gene expression regulation ; genomics ; honey bees ; Kidneys ; Life Sciences ; Microbial Genetics and Genomics ; microRNA ; MicroRNAs ; miRNA ; Nanofiltration ; Next-generation sequencing ; Non-coding RNA ; Original Article ; p53 Protein ; Plant Genetics and Genomics ; Royal jelly ; swine ; Wound healing</subject><ispartof>Functional & integrative genomics, 2023-09, Vol.23 (3), p.200-200, Article 200</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-b1efcc71485584645cdc397c0e7bef62288db7c4deeb329d3b8a38fca3442b4c3</citedby><cites>FETCH-LOGICAL-c408t-b1efcc71485584645cdc397c0e7bef62288db7c4deeb329d3b8a38fca3442b4c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10142-023-01126-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10142-023-01126-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37284890$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saadeldin, Islam M.</creatorcontrib><creatorcontrib>Tanga, Bereket Molla</creatorcontrib><creatorcontrib>Bang, Seonggyu</creatorcontrib><creatorcontrib>Maigoro, Abdulkadir Y.</creatorcontrib><creatorcontrib>Kang, Heejae</creatorcontrib><creatorcontrib>Cha, Dabin</creatorcontrib><creatorcontrib>Lee, Soojin</creatorcontrib><creatorcontrib>Lee, Sanghoon</creatorcontrib><creatorcontrib>Cho, Jongki</creatorcontrib><title>MicroRNA profiling of royal jelly extracellular vesicles and their potential role in cell viability and reversing cell apoptosis</title><title>Functional & integrative genomics</title><addtitle>Funct Integr Genomics</addtitle><addtitle>Funct Integr Genomics</addtitle><description>MiRNAs are small non-coding RNA molecules that play important regulatory roles in diverse biological processes. Royal jelly, a milky-white substance produced by nurse honeybees (
Apis mellifera
), is the primary food of queen bees and plays a crucial role in their development. However, little is known about the microRNA (miRNAs) content of royal jelly and their potential functions. In this study, we isolated extracellular vesicles from the royal jelly of 36 samples through sequential centrifugation and targeted nanofiltration and performed high-throughput sequencing to identify and quantify the miRNA content of honeybee royal jelly extracellular vesicles (RJEVs). We found a total of 29 known mature miRNAs and 17 novel miRNAs. Through bioinformatic analysis, we identified several potential target genes of the miRNAs present in royal jelly, including those involved in developmental processes and cell differentiation. To investigate the potential roles of RJEVs in cell viability, RJEVs were supplemented to apoptotic porcine kidney fibroblasts induced by ethanol 6% exposure for 30 min. TUNEL assay showed a significant reduction in the apoptosis percentage after RJEV supplementation when compared with the non-supplemented control group. Moreover, the wound healing assay performed on the apoptotic cells showed a rapid healing capacity of RJEV-supplemented cells compared to the control group. We observed a significant reduction in the expression of the miRNA target genes such as FAM131B, ZEB1, COL5A1, TRIB2, YBX3, MAP2, CTNNA1, and ADAMTS9 suggesting that RJEVs may regulate the target gene expression associated with cellular motility and cell viability. Moreover, RJEVs reduced the expression of apoptotic genes (CASP3, TP53, BAX, and BAK), while significantly increasing the expression of anti-apoptotic genes (BCL2 and BCL-XL). Our findings provide the first comprehensive analysis of the miRNA content of RJEVs and suggest a potential role for these vesicles in the regulation of gene expression and cell survival as well as augmenting cell resurrection or anastasis.</description><subject>Animal Genetics and Genomics</subject><subject>Apis mellifera</subject><subject>Apoptosis</subject><subject>Bcl-x protein</subject><subject>Bees</subject><subject>Biochemistry</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Cell differentiation</subject><subject>cell movement</subject><subject>Cell survival</subject><subject>Cell viability</subject><subject>Centrifugation</subject><subject>Epigenetics</subject><subject>ethanol</subject><subject>Extracellular vesicles</subject><subject>Fibroblasts</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>genomics</subject><subject>honey bees</subject><subject>Kidneys</subject><subject>Life Sciences</subject><subject>Microbial Genetics and Genomics</subject><subject>microRNA</subject><subject>MicroRNAs</subject><subject>miRNA</subject><subject>Nanofiltration</subject><subject>Next-generation sequencing</subject><subject>Non-coding RNA</subject><subject>Original Article</subject><subject>p53 Protein</subject><subject>Plant Genetics and Genomics</subject><subject>Royal jelly</subject><subject>swine</subject><subject>Wound 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profiling of royal jelly extracellular vesicles and their potential role in cell viability and reversing cell apoptosis</title><author>Saadeldin, Islam M. ; Tanga, Bereket Molla ; Bang, Seonggyu ; Maigoro, Abdulkadir Y. ; Kang, Heejae ; Cha, Dabin ; Lee, Soojin ; Lee, Sanghoon ; Cho, Jongki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-b1efcc71485584645cdc397c0e7bef62288db7c4deeb329d3b8a38fca3442b4c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animal Genetics and Genomics</topic><topic>Apis mellifera</topic><topic>Apoptosis</topic><topic>Bcl-x protein</topic><topic>Bees</topic><topic>Biochemistry</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Cell differentiation</topic><topic>cell movement</topic><topic>Cell survival</topic><topic>Cell viability</topic><topic>Centrifugation</topic><topic>Epigenetics</topic><topic>ethanol</topic><topic>Extracellular vesicles</topic><topic>Fibroblasts</topic><topic>Gene expression</topic><topic>gene expression regulation</topic><topic>genomics</topic><topic>honey bees</topic><topic>Kidneys</topic><topic>Life Sciences</topic><topic>Microbial Genetics and Genomics</topic><topic>microRNA</topic><topic>MicroRNAs</topic><topic>miRNA</topic><topic>Nanofiltration</topic><topic>Next-generation sequencing</topic><topic>Non-coding RNA</topic><topic>Original Article</topic><topic>p53 Protein</topic><topic>Plant Genetics and Genomics</topic><topic>Royal jelly</topic><topic>swine</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saadeldin, Islam M.</creatorcontrib><creatorcontrib>Tanga, Bereket Molla</creatorcontrib><creatorcontrib>Bang, Seonggyu</creatorcontrib><creatorcontrib>Maigoro, Abdulkadir 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saadeldin, Islam M.</au><au>Tanga, Bereket Molla</au><au>Bang, Seonggyu</au><au>Maigoro, Abdulkadir Y.</au><au>Kang, Heejae</au><au>Cha, Dabin</au><au>Lee, Soojin</au><au>Lee, Sanghoon</au><au>Cho, Jongki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA profiling of royal jelly extracellular vesicles and their potential role in cell viability and reversing cell apoptosis</atitle><jtitle>Functional & integrative genomics</jtitle><stitle>Funct Integr Genomics</stitle><addtitle>Funct Integr Genomics</addtitle><date>2023-09-01</date><risdate>2023</risdate><volume>23</volume><issue>3</issue><spage>200</spage><epage>200</epage><pages>200-200</pages><artnum>200</artnum><issn>1438-793X</issn><eissn>1438-7948</eissn><abstract>MiRNAs are small non-coding RNA molecules that play important regulatory roles in diverse biological processes. Royal jelly, a milky-white substance produced by nurse honeybees (
Apis mellifera
), is the primary food of queen bees and plays a crucial role in their development. However, little is known about the microRNA (miRNAs) content of royal jelly and their potential functions. In this study, we isolated extracellular vesicles from the royal jelly of 36 samples through sequential centrifugation and targeted nanofiltration and performed high-throughput sequencing to identify and quantify the miRNA content of honeybee royal jelly extracellular vesicles (RJEVs). We found a total of 29 known mature miRNAs and 17 novel miRNAs. Through bioinformatic analysis, we identified several potential target genes of the miRNAs present in royal jelly, including those involved in developmental processes and cell differentiation. To investigate the potential roles of RJEVs in cell viability, RJEVs were supplemented to apoptotic porcine kidney fibroblasts induced by ethanol 6% exposure for 30 min. TUNEL assay showed a significant reduction in the apoptosis percentage after RJEV supplementation when compared with the non-supplemented control group. Moreover, the wound healing assay performed on the apoptotic cells showed a rapid healing capacity of RJEV-supplemented cells compared to the control group. We observed a significant reduction in the expression of the miRNA target genes such as FAM131B, ZEB1, COL5A1, TRIB2, YBX3, MAP2, CTNNA1, and ADAMTS9 suggesting that RJEVs may regulate the target gene expression associated with cellular motility and cell viability. Moreover, RJEVs reduced the expression of apoptotic genes (CASP3, TP53, BAX, and BAK), while significantly increasing the expression of anti-apoptotic genes (BCL2 and BCL-XL). Our findings provide the first comprehensive analysis of the miRNA content of RJEVs and suggest a potential role for these vesicles in the regulation of gene expression and cell survival as well as augmenting cell resurrection or anastasis.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>37284890</pmid><doi>10.1007/s10142-023-01126-9</doi><tpages>1</tpages></addata></record> |
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| ispartof | Functional & integrative genomics, 2023-09, Vol.23 (3), p.200-200, Article 200 |
| issn | 1438-793X 1438-7948 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Animal Genetics and Genomics Apis mellifera Apoptosis Bcl-x protein Bees Biochemistry Bioinformatics Biomedical and Life Sciences Cell Biology Cell differentiation cell movement Cell survival Cell viability Centrifugation Epigenetics ethanol Extracellular vesicles Fibroblasts Gene expression gene expression regulation genomics honey bees Kidneys Life Sciences Microbial Genetics and Genomics microRNA MicroRNAs miRNA Nanofiltration Next-generation sequencing Non-coding RNA Original Article p53 Protein Plant Genetics and Genomics Royal jelly swine Wound healing |
| title | MicroRNA profiling of royal jelly extracellular vesicles and their potential role in cell viability and reversing cell apoptosis |
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