Combined amplification-based single-molecule fluorescence in situ hybridization with immunofluorescence for simultaneous in situ detection of RNAs and proteins
We present a combined amplification-based single-molecule fluorescence in situ hybridization and immunofluorescence (asmFISH-IF) method for the detection of multiple RNAs and proteins simultaneously in cells and formaldehyde-fixed and paraffin-embedded tissue sections. We showed that performing asmF...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2024-02, Vol.696, p.149508-149508, Article 149508 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Qiu, Yinghui Wei, Kaipeng Lin, Hui Liu, Yanxiu Lin, Chen Ke, Rongqin |
| description | We present a combined amplification-based single-molecule fluorescence in situ hybridization and immunofluorescence (asmFISH-IF) method for the detection of multiple RNAs and proteins simultaneously in cells and formaldehyde-fixed and paraffin-embedded tissue sections. We showed that performing asmFISH before immunofluorescence gives a better IF signal than the opposite. Our asmFISH-IF method could help study the interplay of RNA and protein, helping to understand their functions.
•asmFISH-IF detects RNA and protein in situ simultaneously.•asmFISH-IF can be applied to both fixed cell and tissue sections.•asmFISH-IF offers an alternative option for protein-RNA co-detection.•Performing RNA detection before protein detection gives a better signal. |
| doi_str_mv | 10.1016/j.bbrc.2024.149508 |
| format | Article |
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| ispartof | Biochemical and biophysical research communications, 2024-02, Vol.696, p.149508-149508, Article 149508 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | fluorescence fluorescent antibody technique hybridization Immunofluorescence In situ hybridization Protein RNA Rolling circle amplification |
| title | Combined amplification-based single-molecule fluorescence in situ hybridization with immunofluorescence for simultaneous in situ detection of RNAs and proteins |
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